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General Information
Symbol
Dmel\CyfipEP3267
Species
D. melanogaster
Name
FlyBase ID
FBal0130591
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-CYFIP, EP(3)3267
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{EP}CyfipEP3267
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous Sra-1EP3267 mutant adults display grossly normal eye morphology, but show subtle anomalies in ommatidia organisation with low penetrance.

Sra-1EP3267/Sra-185.1 mutant pharate adults have rough eyes and display dark lenses in a speckled, random pattern indicating cone cell death. The ommatidia are occasionally misaligned or fused to their neighbours and some lack the typical smooth surface, instead showing crater-like intrusions indicative of lens material loss. Interommatidial bristles are often bent and appear soft and flat. Rhabdomeres appear bulky and never span the entire retina, either accumulating near the distal surface or underneath the retinal base (FM) in the lamina. The FM is mispositioned, leading to a markedly reduced retina depth. The rhabdomeres appear bulky and never span the entire retina, accumulating near the distal surface or underneath the FM in the lamina. The actin organisation of the FM is compromised and the polarised accumulation of dense cortical actin latices near the inner and outer lateral membrane of pigment cells is abolished.

Over 30% of Sra-1EP3267/Sra-185.1 pupal photoreceptors possess ectopic membrane protrusions projecting from the stalks, a phenomenon never observed in wild type. Gaps also occur between the rhabdomeres and the cytoplasm and the rhabdomeres themselves comprise a massive amount of membrane that occupies an area that is twice as large as in controls, however the space occupied by the actin rich rhabdomere terminal web (RTW) is not proportionally increased. Actin fingers that normally extend from the sub-rhabdomeric space into the RTW are absent in Sra-1EP3267/Sra-185.1 mutant cells but microvillar structure and stacking appear normal. Adherens junctions are correctly specified but structurally compromised. Defects in cone cell shape and configuration are also seen.

50% of animals die before eclosion. When Sra-1EP3267 is driven in the eye by Scer\GAL4elav-C155, no effects are seen on eye morphology.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Phenotype Manifest In
NOT Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The reduced synaptic length seen at the neuromuscular junction in larvae expressing Fmr1EP3517 under the control of Scer\GAL4elav-C155 is partially suppressed by co-expression of Sra-1EP3267.

The combination of Sra-1EP3267 and Rac1V12.Scer\UAS driven by Scer\GAL4GMR.PF has a weaker eye phenotype than Rac1V12.Scer\UAS (driven by Scer\GAL4GMR.PF) alone. The combination of Sra-1EP3267 and Rac1V12.Scer\UAS driven by Scer\GAL4elav-C155 has a ISNb stalling phenotype (only 17% exhibit the phenotype compared to 29%) than Rac1V12.Scer\UAS (driven by Scer\GAL4elav-C155) alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of Sra-1EP3267 under the control of Scer\GAL4GMR.PF fully rescues the eye morphology defects seen in Sra-1EP3267/Sra-185.1 mutant pharate adults. The lethality associated with Sra-1EP3267/Sra-185.1 is not rescued.

Expression of Sra-1EP3267 under the control of Scer\GAL4lz-gal4 fully rescues the retinal fenestrated membrane integrity defects seen in Sra-1EP3267/Sra-185.1 mutant pharate adults. As result photoreceptors are kept in place and no longer fall into the lamina. The dark necrotic lenses seen in mutant flies are also absent. Some bulky rhabdomeres are still seen, but fewer than are seen in the mutant alone.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
CyfipEP3267
Sra-1EP3267
Name Synonyms
Secondary FlyBase IDs
    References (6)