FlyBase Allele Report: Scer\GAL4[Ubi.PU]

General Information

Symbol

Scer\GAL4^Ubi.PU

Species

S. cerevisiae

Name

Ubiquitin promoter construct of Unknown

FlyBase ID

FBal0130678

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Ubi-GAL4.U}

Also Known As

Ubi-Gal4, ubiquitin-GAL4, UbiGal4, ub-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Schulz et al., 2001)

Progenitor genotype

Carried in construct

P{Ubi-GAL4.U}

Cytology

Description

A ubiquitin promoter regulates expression of a GAL4 driver.

(Schulz et al., 2001)

Allele components

Component

Use(s)

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage -- adult stage

organism | ubiquitous

(Bloomington Drosophila Stock Center, 2017)

Additional Information

Statement

Reference

ScerGAL4^Ubi.PU drives expression all cells.

(Bloomington Drosophila Stock Center, 2017)

Drives expression in all cells.

(FlyBase Curators, 2017)

Marker for

Reflects expression of

Ubi-p5E

(Bloomington Drosophila Stock Center, 2017)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with vito^GD10890

(Marinho et al., 2011)

abnormal feeding behavior, with AMPKα^K57A.UAS

(Johnson et al., 2010)

abnormal feeding behavior, with SNF4Aγ^RNAi.UAS

(Johnson et al., 2010)

abnormal immune response | adult stage, with acs^KK111566

(Socha et al., 2023)

abnormal immune response | adult stage, with BaraA2^HM05004

(Huang et al., 2023)

abnormal immune response | adult stage, with CheA75a^KK104343

(Shang et al., 2023)

abnormal immune response | adult stage, with Df(3R)Exel6209, Sid^NIG.9989R

(Seong et al., 2014)

abnormal immune response | adult stage, with Sid^NIG.9989R

(Seong et al., 2014)

abnormal locomotor behavior | adult stage, with AIF^GD822

(Murari et al., 2020)

abnormal locomotor behavior | third instar larval stage, with Arp2^GD14716

(Koch et al., 2014)

abnormal locomotor behavior | third instar larval stage, with Arp3^GD12273

(Koch et al., 2014)

abnormal locomotor rhythm | nutrition conditional, with AMPKα^K57A.UAS

(Johnson et al., 2010)

abnormal locomotor rhythm | nutrition conditional, with SNF4Aγ^RNAi.UAS

(Johnson et al., 2010)

abnormal neuroanatomy | larval stage, with Arp2^GD14716

(Koch et al., 2014)

abnormal neuroanatomy | larval stage, with Arp3^GD12273

(Koch et al., 2014)

abnormal oxidative stress response | adult stage, with Df(3R)Exel6209, Sid^NIG.9989R

(Seong et al., 2014)

abnormal oxidative stress response | adult stage, with Sid^NIG.9989R

(Seong et al., 2014)

abnormal starvation stress response, with AMPKα^K57A.UAS

(Johnson et al., 2010)

abnormal starvation stress response, with SNF4Aγ^RNAi.UAS

(Johnson et al., 2010)

chemical sensitive, with BaraA2^HM05004

(Huang et al., 2023)

increased mortality | chemical conditional, with BaraA2^HM05004

(Huang et al., 2023)

increased mortality | conditional, with BaraA2^HM05004

(Huang et al., 2023)

increased mortality | conditional, with CheA75a^KK104343

(Shang et al., 2023)

increased rate of movement | nutrition conditional, with AMPKα^K57A.UAS

(Johnson et al., 2010)

increased rate of movement | nutrition conditional, with SNF4Aγ^RNAi.UAS

(Johnson et al., 2010)

lethal, with bor^R534W.UAS

(Harel et al., 2016)

lethal, with CG42340^KK113126

(Dembeck et al., 2015)

lethal, with Dronc^KK104278

(Dembeck et al., 2015)

lethal, with eIF4G1^HMS00762

(Ghosh and Lasko, 2015)

lethal, with kay^GD1469

(Dembeck et al., 2015)

lethal, with kkv^KK105928

(Dembeck et al., 2015)

lethal, with Klp61F^KK100504

(Dembeck et al., 2015)

lethal, with Mes2^UAS.cZa

(Zimmermann et al., 2006)

lethal, with pns^KK101479

(Dembeck et al., 2015)

lethal, with Psa^UAS.cSa

(Schulz et al., 2001)

lethal, with ru^KK105434

(Dembeck et al., 2015)

lethal, with sinu^GD2315

(Dembeck et al., 2015)

lethal, with Vmat^KK112993

(Dembeck et al., 2015)

lethal - all die during embryonic stage, with Hsap\EWSR1::Hsap\FLI1^1FS.UAS

(Molnar et al., 2022)

lethal - all die during P-stage, with SCCRO4^VDRC.cUa

(Fu et al., 2016)

lethal - all die during pupal stage, with Sprn^UAS.GFP

(Chen and Megraw, 2014)

majority die during larval stage | nutrition conditional, with Drep2^GD9693

(Havula et al., 2022)

majority die during larval stage | nutrition conditional, with Eip63E^GD16928

(Havula et al., 2022)

majority die during larval stage | nutrition conditional, with Smr^KK102611

(Havula et al., 2022)

majority die during P-stage | nutrition conditional, with alien^GD4682

(Havula et al., 2022)

majority die during P-stage | nutrition conditional, with grn^KK112480

(Havula et al., 2022)

majority die during P-stage | nutrition conditional, with Sply^KK105752

(Havula et al., 2022)

majority die during P-stage | nutrition conditional, with tll^GD1548

(Havula et al., 2022)

male semi-fertile, with lncRNA:CR44456^RNAi.UAS.1

(Wen et al., 2016)

partially lethal | heat sensitive, with AMPKα^K57A.UAS

(Onyenwoke et al., 2015)

partially lethal - majority die | nutrition conditional, with alien^GD4682

(Havula et al., 2022)

partially lethal - majority die | nutrition conditional, with Drep2^GD9693

(Havula et al., 2022)

partially lethal - majority die | nutrition conditional, with Eip63E^GD16928

(Havula et al., 2022)

partially lethal - majority die | nutrition conditional, with grn^KK112480

(Havula et al., 2022)

partially lethal - majority die | nutrition conditional, with Smr^KK102611

(Havula et al., 2022)

partially lethal - majority die | nutrition conditional, with Sply^KK105752

(Havula et al., 2022)

partially lethal - majority die | nutrition conditional, with tll^GD1548

(Havula et al., 2022)

semi-fertile, with Kdm5¹⁴⁰, Kdm5^UASp.cSa

(Drelon et al., 2019)

short lived, with AIF^GD822

(Murari et al., 2020)

short lived, with AMPKα^K57A.UAS

(Johnson et al., 2010)

short lived, with SNF4Aγ^RNAi.UAS

(Johnson et al., 2010)

viable, with bor^WT.UAS

(Harel et al., 2016)

viable, with Caf1-55^UAS.cAa

(Anderson et al., 2011)

viable, with SCCRO3^VDRC.cUa

(Fu et al., 2016)

viable, with SCCRO^GD12234

(Fu et al., 2016)

viable, with SCCRO^KK102723

(Fu et al., 2016)

visible | cold sensitive, with Caf1-55^UAS.cAa, Caf1-55^med/Caf1-55^short

(Anderson et al., 2011)

wild-type, with Caf1-55^UAS.cAa

(Anderson et al., 2011)

Phenotype Manifest In

abdominal tergite 5 | female, with dsb^KK107147

(Dembeck et al., 2015)

abdominal tergite 5 | female, with klar^GD9271

(Dembeck et al., 2015)

abdominal tergite 6 | female, with klar^GD9271

(Dembeck et al., 2015)

arista | cold sensitive, with Caf1-55^UAS.cAa, Caf1-55^med/Caf1-55^short

(Anderson et al., 2011)

embryonic/larval neuromuscular junction | larval stage, with Arp2^GD14716

(Koch et al., 2014)

embryonic/larval neuromuscular junction | larval stage, with Arp3^GD12273

(Koch et al., 2014)

embryonic dorsal epidermis | dorsal closure stage, with Mes2^UAS.cZa

(Zimmermann et al., 2006)

eye | cold sensitive, with Caf1-55^UAS.cAa, Caf1-55^med/Caf1-55^short

(Anderson et al., 2011)

gut epithelium | adult stage, with acs^KK111566

(Socha et al., 2023)

leg | ectopic | cold sensitive, with Caf1-55^UAS.cAa, Caf1-55^med/Caf1-55^short

(Anderson et al., 2011)

oenocyte, with AMPKα^K57A.UAS

(Johnson et al., 2010)

oenocyte, with SNF4Aγ^RNAi.UAS

(Johnson et al., 2010)

rhabdomere | cold sensitive, with Caf1-55^UAS.cAa, Caf1-55^med/Caf1-55^short

(Anderson et al., 2011)

spermatid, with lncRNA:CR43416^RNAi.UAS

(Wen et al., 2016)

spermatid, with lncRNA:CR44585^RNAi.UAS

(Wen et al., 2016)

spermatozoon, with lncRNA:CR43416^RNAi.UAS

(Wen et al., 2016)

spermatozoon, with lncRNA:CR44585^RNAi.UAS

(Wen et al., 2016)

type Ib terminal bouton | larval stage, with Arp2^GD14716

(Koch et al., 2014)

type Ib terminal bouton | larval stage, with Arp3^GD12273

(Koch et al., 2014)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Ack^UAS.Tag:V5/Scer\GAL4^Ubi.PU is an enhancer of visible | adult stage | somatic clone phenotype of hpo^BF33

(Hu et al., 2016)

NOT Enhancer of

Statement

Reference

Scer\GAL4^Ubi.PU/Ack^{KD.UAS.Tag:V5} is a non-enhancer of visible | adult stage | somatic clone phenotype of hpo^BF33

(Hu et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^Ubi.PU/BaraA2^UAS.cHb is a suppressor | partially of increased mortality | conditional phenotype of Myd88^unspecified

(Huang et al., 2023)

Scer\GAL4^Ubi.PU/BaraA2^UAS.cHb is a suppressor | partially of abnormal immune response | adult stage phenotype of Myd88^unspecified

(Huang et al., 2023)

Scer\GAL4^Ubi.PU/Hsap\WAC^UAS.cDa is a suppressor of lethal - all die during second instar larval stage phenotype of wcy^A

(David-Morrison et al., 2016)

NOT Suppressor of

Statement

Reference

Ack^UAS.Tag:V5/Scer\GAL4^Ubi.PU is a non-suppressor of visible | somatic clone | adult stage phenotype of ex^e1

(Hu et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

Ack^UAS.Tag:V5/Scer\GAL4^Ubi.PU is an enhancer of eye | adult stage | somatic clone phenotype of hpo^BF33

(Hu et al., 2016)

NOT Enhancer of

Statement

Reference

Ack^UAS.Tag:V5/Scer\GAL4^Ubi.PU is a non-enhancer of eye | adult stage | somatic clone phenotype of ex^e1

(Hu et al., 2016)

Ack^UAS.Tag:V5/Scer\GAL4^Ubi.PU is a non-enhancer of antenna | adult stage | somatic clone phenotype of ex^e1

(Hu et al., 2016)

Scer\GAL4^Ubi.PU/Ack^{KD.UAS.Tag:V5} is a non-enhancer of eye | adult stage | somatic clone phenotype of hpo^BF33

(Hu et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^Ubi.PU/Pi3K92E^JF02770 is a suppressor | partially of ommatidium | somatic clone phenotype of Pten³

(Malin et al., 2024)

Scer\GAL4^Ubi.PU/Pi3K92E^JF02770 is a suppressor | partially of interommatidial cell | somatic clone phenotype of Pten³

(Malin et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Ubi.PU/Pten^{UAS.2.cMa.GFP} rescues Pten³

(Malin et al., 2024)

Scer\GAL4^Ubi.PU/BaraA2^UAS.cHb rescues BaraA2^KO1

(Huang et al., 2023)

Wdr24^UASp.EGFP/Scer\GAL4^Ubi.PU rescues Wdr24¹

(Cai et al., 2016)

Scer\GAL4^Ubi.PU/wcy^UAS.cDa rescues wcy^A

(David-Morrison et al., 2016)

Scer\GAL4^Ubi.PU/wcy^UAS.Tag:FLAG rescues wcy^A

(David-Morrison et al., 2016)

Scer\GAL4^Ubi.PU/wcy^UAS.cDa rescues wcy^B

(David-Morrison et al., 2016)

Scer\GAL4^Ubi.PU/wcy^UAS.Tag:FLAG rescues wcy^B

(David-Morrison et al., 2016)

Scer\GAL4^Ubi.PU/wcy^UAS.cDa rescues wcy^C

(David-Morrison et al., 2016)

Scer\GAL4^Ubi.PU/wcy^UAS.Tag:FLAG rescues wcy^C

(David-Morrison et al., 2016)

Scer\GAL4^Ubi.PU/wcy^UAS.cDa rescues wcy^D

(David-Morrison et al., 2016)

Scer\GAL4^Ubi.PU/wcy^UAS.Tag:FLAG rescues wcy^D

(David-Morrison et al., 2016)

Scer\GAL4^Ubi.PU/wcy^UAS.cDa rescues wcy^F

(David-Morrison et al., 2016)

Scer\GAL4^Ubi.PU/wcy^UAS.Tag:FLAG rescues wcy^F

(David-Morrison et al., 2016)

Scer\GAL4^Ubi.PU/Als2^UAS.Tag:HA rescues Als2^Ex44

(Takayama et al., 2014)

Spase12^UAS.cHGa/Scer\GAL4^Ubi.PU rescues Df(3R)Spase12^d4/Spase12^C24

(Haase Gilbert et al., 2013)

Scer\GAL4^Ubi.PU/AMPKα^UAS.mCherry rescues AMPKα¹

(Swick et al., 2013)

AMPKα^UAS.cMa/Scer\GAL4^Ubi.PU rescues AMPKα¹

(Swick et al., 2013)

Scer\GAL4^Ubi.PU/AMPKα^UAS.mCherry rescues AMPKα³

(Swick et al., 2013)

AMPKα^UAS.cMa/Scer\GAL4^Ubi.PU rescues AMPKα³

(Swick et al., 2013)

Scer\GAL4^Ubi.PU/Caf1-55^UAS.cAa rescues Caf1-55^short/Caf1-55^long

(Anderson et al., 2011)

park^UAS.Tag:HA/Scer\GAL4^Ubi.PU rescues park^Δ21

(Pesah et al., 2004)

Partially rescues

Scer\GAL4^Ubi.PU/Pten^{C132S.UAS.2.GFP} partially rescues Pten³

(Malin et al., 2024)

Pten^{G137E.UAS.2.GFP}/Scer\GAL4^Ubi.PU partially rescues Pten³

(Malin et al., 2024)

Scer\GAL4^Ubi.PU/Kdm5^UASp.cSa partially rescues Kdm5¹⁴⁰

(Drelon et al., 2019)

E2f1^{UAS.a.Tag:FLAG,Tag:MYC}/Scer\GAL4^Ubi.PU partially rescues Df(3R)Exel6186/E2f1^rM729

(Kim et al., 2018)

E2f1^{UAS.a.Tag:FLAG,Tag:MYC}, Scer\GAL4^Ubi.PU, E2f1^{UAS.b.Tag:FLAG,Tag:MYC} partially rescues Df(3R)Exel6186/E2f1^rM729

(Kim et al., 2018)

Scer\GAL4^Ubi.PU/E2f1^{UAS.b.Tag:FLAG,Tag:MYC} partially rescues Df(3R)Exel6186/E2f1^rM729

(Kim et al., 2018)

Scer\GAL4^Ubi.PU/Caf1-55^UAS.cAa partially rescues Caf1-55^med/Caf1-55^short

(Anderson et al., 2011)

Scer\GAL4^Ubi.PU/Caf1-55^UAS.cAa partially rescues Caf1-55^short

(Anderson et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

32551

w^*; P{Ubi-GAL4.U}2/CyO

Notes on Origin

Discoverer

Comments

Carried in plasmid "ub-Gal4", transfected into S2 cells and used as a driver construct in studies of mirtron biogenesis.

(Okamura et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scer\GAL4^Ubi.PU

Scer\GAL4^Ubi

Name Synonyms

Ubiquitin promoter construct of Unknown

Secondary FlyBase IDs

References (83)

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