Allele Scer\GAL4^Ubi

General Information
Symbol	Scer\GAL4^Ubi	Species	S. cerevisiae
Name		FlyBase ID	FBal0130678
Feature type	allele	Associated gene	Scer\GAL4

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Schulz et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A ubiquitin promoter drives expression of Scer\GAL4. (Schulz et al., 2001)
Carried in construct	P{Ubi-GAL4} (Schulz et al., 2001, Kwon et al., 2005, Pesah et al., 2004, Anderson et al., 2011, Mirouse et al., 2007, Zimmermann et al., 2006, Kazgan et al., 2010, Marinho et al., 2011, Silver et al., 2007, Johnson et al., 2010, Pircs et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	developmental rate defective, with vito^GD10890 (Marinho et al., 2011) feeding behavior defective, with SNF1A^{K57A.Scer\UAS} (Johnson et al., 2010) feeding behavior defective, with SNF4Aγ^{dsRNA.Scer\UAS} (Johnson et al., 2010) hyperactive \| nutrition conditional, with SNF1A^{K57A.Scer\UAS} (Johnson et al., 2010) hyperactive \| nutrition conditional, with SNF4Aγ^{dsRNA.Scer\UAS} (Johnson et al., 2010) lethal, with Mes2^Scer\UAS.cZa (Zimmermann et al., 2006) lethal, with Psa^Scer\UAS.cSa (Schulz et al., 2001) locomotor rhythm defective \| nutrition conditional, with SNF1A^{K57A.Scer\UAS} (Johnson et al., 2010) locomotor rhythm defective \| nutrition conditional, with SNF4Aγ^{dsRNA.Scer\UAS} (Johnson et al., 2010) short lived, with SNF1A^{K57A.Scer\UAS} (Johnson et al., 2010) short lived, with SNF4Aγ^{dsRNA.Scer\UAS} (Johnson et al., 2010) starvation stress response defective, with SNF1A^{K57A.Scer\UAS} (Johnson et al., 2010) starvation stress response defective, with SNF4Aγ^{dsRNA.Scer\UAS} (Johnson et al., 2010) viable, with Caf1^Scer\UAS.cAa (Anderson et al., 2011) visible \| cold sensitive, with Caf1^Scer\UAS.cAa, Caf1^med/Caf1^short (Anderson et al., 2011) wild-type, with Caf1^Scer\UAS.cAa (Anderson et al., 2011)
Phenotype Manifest In
	arista \| cold sensitive, with Caf1^Scer\UAS.cAa, Caf1^med/Caf1^short (Anderson et al., 2011) embryonic dorsal epidermis \| dorsal closure stage, with Mes2^Scer\UAS.cZa (Zimmermann et al., 2006) eye \| cold sensitive, with Caf1^Scer\UAS.cAa, Caf1^med/Caf1^short (Anderson et al., 2011) leg \| ectopic \| cold sensitive, with Caf1^Scer\UAS.cAa, Caf1^med/Caf1^short (Anderson et al., 2011) oenocyte, with SNF1A^{K57A.Scer\UAS} (Johnson et al., 2010) oenocyte, with SNF4Aγ^{dsRNA.Scer\UAS} (Johnson et al., 2010) rhabdomere \| cold sensitive, with Caf1^Scer\UAS.cAa, Caf1^med/Caf1^short (Anderson et al., 2011)
Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	park^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^Ubi rescues park^Δ21 (Pesah et al., 2004) Scer\GAL4^Ubi/Caf1^Scer\UAS.cAa rescues Caf1^short/Caf1^long (Anderson et al., 2011) Scer\GAL4^Ubi/SNF1A^{Scer\UAS.T:Disc\RFP-mCherry} rescues SNF1A¹ (Mirouse et al., 2007) Scer\GAL4^Ubi/SNF1A^{Scer\UAS.T:Disc\RFP-mCherry} rescues SNF1A³ (Mirouse et al., 2007)
Partially rescues	Scer\GAL4^Ubi/Caf1^Scer\UAS.cAa partially rescues Caf1^med/Caf1^short (Anderson et al., 2011) Scer\GAL4^Ubi/Caf1^Scer\UAS.cAa partially rescues Caf1^short (Anderson et al., 2011)
Comments
Stocks ( 1 )
Bloomington	32551 w^*; P{Ubi-GAL4}2/CyO
Notes on Origin
Discoverer

Comments
	Carried in plasmid "ub-Gal4", transfected into S2 cells and used as a driver construct in studies of mirtron biogenesis. (Okamura et al., 2007)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Scer\GAL4^Ubi
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Pircs et al., 2012, PLoS ONE 7(8): e44214 Advantages and Limitations of Different p62-Based Assays for Estimating Autophagic Activity in Drosophila. [FBrf0219364] Anderson et al., 2011, Development 138(10): 1957--1966 The enhancer of trithorax and polycomb gene Caf1/p55 is essential for cell survival and patterning in Drosophila development. [FBrf0213580] Marinho et al., 2011, Development 138(2): 349--357 The Drosophila Nol12 homologue viriato is a dMyc target that regulates nucleolar architecture and is required for dMyc-stimulated cell growth. [FBrf0212565] Johnson et al., 2010, PLoS ONE 5(9): e12799 Altered metabolism and persistent starvation behaviors caused by reduced AMPK function in Drosophila. [FBrf0211905] Kazgan et al., 2010, Mol. Biol. Cell 21(19): 3433--3442 Identification of a nuclear export signal in the catalytic subunit of AMP-activated protein kinase. [FBrf0211940] Mirouse et al., 2007, J. Cell Biol. 177(3): 387--392 LKB1 and AMPK maintain epithelial cell polarity under energetic stress. [FBrf0201625] Okamura et al., 2007, Cell 130(1): 89--100 The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila. [FBrf0200988] Silver et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(46): 18151--18156 Functional screening identifies miR-315 as a potent activator of Wingless signaling. [FBrf0202770] Zimmermann et al., 2006, Dev. Dyn. 235(12): 3387--3395 Mes2, a MADF-containing transcription factor essential for Drosophila development. [FBrf0194335] Kwon et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(52): 18986--18991 MicroRNA1 influences cardiac differentiation in Drosophila and regulates Notch signaling. [FBrf0191448] Pesah et al., 2004, Development 131(9): 2183--2194 Drosophila parkin mutants have decreased mass and cell size and increased sensitivity to oxygen radical stress. [FBrf0174577] Schulz et al., 2001, Dev. Genes Evol. 211(12): 581--588 Genetic analysis of dPsa, the Drosophila orthologue of puromycin-sensitive aminopeptidase, suggests redundancy of aminopeptidases. [FBrf0144810]

Allele Scer\GAL4Ubi

Allele Scer\GAL4^Ubi