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General Information
Symbol
Dmel\RalaPG69
Species
D. melanogaster
Name
FlyBase ID
FBal0130802
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
PG69
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion in the 5' untranslated region.

Insertion in the first exon.

Insertion components
P{GawB}RalaPG69
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mosaic stage 10 RalaPG69 oocytes show a complete lack of border cell migration.

Mosaic RalaPG69 clones where the outer border cells are mutant but the two polar cells are wildtype show normal formation of the border cell cluster, and normal border cell migration.

When one of two polar cells is mutant for RalaPG69, a reduced number of border cells are seen in the cluster, and it does not migrate.

When both of the polar cells are mutant for RalaPG69, almost no outer border cells are recruited.

Some polar cells, but not other mutant follicle cells, show increased cell death when homozygous mutant for RalaPG69.

Adult hemizygous males die during embryogenesis from stage 13 through the first-instar larval stage.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Suppressor of
Statement
Reference

Rala[+]/RalaPG69 is a suppressor of visible phenotype of lqfFDD9

Phenotype Manifest In
Suppressor of
Statement
Reference

Rala[+]/RalaPG69 is a suppressor of ommatidium phenotype of lqfFDD9

Rala[+]/RalaPG69 is a suppressor of eye photoreceptor cell phenotype of lqfFDD9

Additional Comments
Genetic Interactions
Statement
Reference

RalaPG69 dominantly suppresses the rough eye phenotype of lqfFDD9 homozygotes, with a greater proportion of ommatidia containing the normal number of photoreceptor cells in the double mutant flies.

Expression of JraDN.Scer\UAS under the control of Scer\GAL4Rala-PG69 rescues RalaPG69 hemizygous males to the pharate adult stage.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of RalaScer\UAS.cSa under the control of Scer\GAL4Rala-PG69 in a RalaPG69 mutant background rescues lethality, and border cell migration.

Expression of RalaG20V.Scer\UAS under the control of Scer\GAL4Rala-PG69 in a RalaPG69 mutant background rescues lethality, and border cell migration.

Expression of RalaS25N.Scer\UAS under the control of Scer\GAL4Rala-PG69 in a RalaPG69 mutant background does not rescue lethality and border cell migration.

Expression of RalaN.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Rala-PG69 in a RalaPG69 mutant background rescues border cell migration.

Expression of RalaΔCTLL.N.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Rala-PG69 in a RalaPG69 mutant background does not rescue border cell migration.

Expression of RalaC.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Rala-PG69 in a RalaPG69 mutant background does not rescue border cell migration.

Expression of RalaScer\UAS.cMa under the control of Scer\GAL4da.G32 restores viability in RalaPG69 hemizygous males.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Precise excision of the insertion reverts the lethal phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (5)