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General Information
Symbol
Dmel\PGRP-SAseml
Species
D. melanogaster
Name
FlyBase ID
FBal0130837
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
seml
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:

    G11562211A

    Reported nucleotide change:

    G174A

    Amino acid change:

    C80Y | PGRP-SA-PA; C80Y | PGRP-SA-PB

    Reported amino acid change:

    C80Y

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Amino acid replacement: C80Y. Nucleotide substitution: G174A. The mutation is within the PGRP domain.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    PGRP-SAseml mutant flies show reduced survival rate upon infection with either Staphylococcus aureus or Streptococcus pyogenes compared to wild-type.

    PGRP-SAseml mutant flies show significantly decreased survival rate upon infection with the Staphylococcus aureus strain COL and unlike wild-type flies is strongly susceptible even to infection with the strain COL MIN, which shows attenuated virulence and is unable to grow in healthy wild-type flies but grows normally in the immunocompromised PGRP-SAseml mutants.

    PGRP-SAseml mutant flies show an increased susceptibility to challenge by Staphylococcus aureus or Enterococcus faecalis compared to wild type flies.

    Mutant flies are susceptible to S. aureus infection compared to wild-type adults.

    Hemolymph clots from PGRP-SAseml third instar larvae show normal melanization.

    PGRP-SAseml mutant adults phagocytose E.coli normally, but phagocytosis of S.aureus is reduced.

    Mutant flies show similar survival rates as control flies following natural infection (feeding with contaminated food) with either "Ecc-15" (P.carotovorum.carotovorum), S.cerevisiae or M.luteus.

    PGRP-SAseml flies show a normal survival rate after natural infection with "Ecc15" (P.carotovorum.carotovorum).

    Mutant flies show reduced survival compared to wild-type controls after infection with either S. pyogenes or S. aureus.

    PGRP-SAseml flies are susceptible to infection by Gram-positive bacteria (E.faecalis, S.aureus or M.luteus). PGRP-SAseml flies are resistant to fungal infection (A.fumigatus or B.bassiana) and to infection by Gram-negative bacteria (P.carotovorum.carotovorum).

    Mutants are highly susceptible to Gram-positive infection, but are resistant as wild-type to fungal and Gram-negative bacterial infection.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    NOT Enhanced by
    NOT suppressed by
    Statement
    Reference
    NOT Enhancer of
    NOT Suppressor of
    Statement
    Reference

    PGRP-SAseml is a non-suppressor of melanotic necrosis phenotype of nec1/nec2

    Phenotype Manifest In
    NOT Enhancer of
    Statement
    Reference

    PGRP-SAseml is a non-enhancer of adult epidermis phenotype of nec1/nec2

    NOT Suppressor of
    Statement
    Reference
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The reduced survival rate of PGRP-SAseml mutant adults upon infection with either Staphylococcus aureus or Streptococcus pyogenes is not worsened further by combination with PGRP-SDsk1.

    The melanisation of the adult tracheal system caused by expression of Spn77BadsRNA.WIZ.Scer\UAS under the control of Scer\GAL4btl.PS is not suppressed by PGRP-SAseml.

    PGRP-SDΔ3 PGRP-SAseml and GNBP1e03371 PGRP-SAseml double mutant flies are extremely susceptible to S. aureus infection compared to wild-type adults.

    Flies expressing GNBP1dsRNA.Scer\UAS under the control of Scer\GAL4da.G32 in a PGRP-SAseml background show the same degree of susceptibility to M.luteus as either PGRP-SAseml single mutant flies or flies expressing GNBP1dsRNA.Scer\UAS under the control of Scer\GAL4da.G32 in a wild-type background.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    Comments
    Comments

    Etymology: The mutation semmelweis is named after Ignaz Philipp Semmelweis, a Hungarian physician who was a pioneer in the field of antiseptic treatments and discovered the cause of puerperal fever.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    References (27)