Open Close
General Information
Symbol
Dmel\MoePL106
Species
D. melanogaster
Name
FlyBase ID
FBal0130904
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{lacW}MoePL106
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Adult escapers of MoePL106 appear at low frequency. Wings of these escapers often appear crumpled (displaying cuticle vesicles trapped between the two wing surfaces) and reduced in size and their eyes display a rough-eye phenotype, because ommatidia are reduced in number and appear disorganized. While expression of MoeTA.Scer\UAS.T:Avic\GFP-EGFP using Scer\GAL4αTub84B.PL fully rescues the wing phenotypes of MoePL106, it only partially rescues the viability, and fails to rescue the eye phenotypes. Expression of MoeKN.Scer\UAS.P\T.T:Avic\GFP using Scer\GAL4αTub84B.PL slightly enhances the viability of MoePL106, however, the escapers display rough eyes and crumpled wings. While expression of MoeKNTD.Scer\UAS.P\T.T:Avic\GFP using Scer\GAL4αTub84B.PL rescues the external wing phenotypes and enhances the viability of MoePL106, it fails to rescue the eye phenotypes. MoePL106 mutant clones induce ectopic cell death in the wing disc epithelium. Expression of MoeKNTD.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4αTub84B.PL fails to rescue the ectopic cell death induced by somatic clones of MoePL106.
Wing discs from moePL106 homozygotes become progressively disorganised during growth. Early third instar discs show a more normal appearance but alterations in the basal lamina are already present at this stage. By late third instar, the disc is misfolded and has breaks in the basal lamina. Defects in the hinge region including mislocalisation of apical markers, misfolding folding and disorganisation of the basal lamina are more severe than those seen in Moec858 mutants. There is also an increase in cell death in the hinge region.
A proportion of the eggs laid by heterozygous MoePL106 heterozygous females display segmentation defects (12%, n=223). These abnormal embryos lack posterior structures. No sign of premature cytoplasmic streaming is seen in MoePL106 germline clone oocytes. However, defects are apparent in the actin cytoskeleton: microfilaments are more loosely bound to the posterior and lateral cortex of the oocyte than in wild-type, and are also sometimes found clumped together outside of the cortex. These defects are not seen in wild-type oocytes overlain by MoePL106 mutant follicle cells. In MoePL106 germline clone nurse cells, actin filaments are often absent from the cortex and concentrated at the cell perimeter. They also accumulate in abnormal structures at the point of contact between nurse cells.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Expression of MoeWT.Scer\UAS.T:Avic\GFP-EGFP using Scer\GAL4αTub84B.PL enhances the viability and completely rescues the wing and eye phenotypes of MoePL106 mutants. While expression of MoeTA.Scer\UAS.T:Avic\GFP-EGFP using Scer\GAL4αTub84B.PL fully rescues the wing phenotypes of MoePL106, it only partially rescues the viability, and fails to rescue the eye phenotypes. Expression of MoeKN.Scer\UAS.P\T.T:Avic\GFP using Scer\GAL4αTub84B.PL slightly enhances the viability of MoePL106, however, the escapers display rough eyes and crumpled wings. While expression of MoeKNTD.Scer\UAS.P\T.T:Avic\GFP using Scer\GAL4αTub84B.PL rescues the external wing phenotypes and enhances the viability of MoePL106, it fails to rescue the eye phenotypes. Expression of MoeKNTD.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4αTub84B.PL fails to rescue the ectopic cell death induced by somatic clones of MoePL106.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
Re-mobilisation of the transposon causes the lethality phenotype to be lost.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (4)