Wing discs from moePL106
homozygotes become progressively disorganised during growth. Early third instar discs show a more normal appearance but alterations in the basal lamina are already present at this stage. By late third instar, the disc is misfolded and has breaks in the basal lamina. Defects in the hinge region including mislocalisation of apical markers, misfolding folding and disorganisation of the basal lamina are more severe than those seen in Moec858
mutants. There is also an increase in cell death in the hinge region.
A proportion of the eggs laid by heterozygous MoePL106
heterozygous females display segmentation defects (12%, n=223). These abnormal embryos lack posterior structures. No sign of premature cytoplasmic streaming is seen in MoePL106
germline clone oocytes. However, defects are apparent in the actin cytoskeleton: microfilaments are more loosely bound to the posterior and lateral cortex of the oocyte than in wild-type, and are also sometimes found clumped together outside of the cortex. These defects are not seen in wild-type oocytes overlain by MoePL106
mutant follicle cells. In MoePL106
germline clone nurse cells, actin filaments are often absent from the cortex and concentrated at the cell perimeter. They also accumulate in abnormal structures at the point of contact between nurse cells.