Open Close
General Information
Symbol
Dmel\LIMK1UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0130965
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS sequences drive expression of LIMK1 tagged with Tag:HA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mushroom bodies expressing LIMK1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey-OK107 display short α/β axonal lobes compared with those of controls, with increased levels of F-actin. Clones expressing LIMK1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey-OK107 also show increased levels of F-actin and both dorsal and medial axon growth defects.

Expression of LIMK1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in olfactory receptor neuron (ORN) clones results in 88% of Or59c-expressing ORNs mistargeting.

Expression of LIMK1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4OK107 results in axon growth defects in the mushroom body, with the severity of the phenotype depending on the particular P{UAS-LIMK1.T:Ivir\HA1} insertion line used. In "severe" cases, all mushroom body axon lobes are truncated and there are axon guidance defects at the peduncle, with axons forming ball-like structures in this region. In "strong" cases, no guidance defects are seen, but both the dorsal and medial branches are truncated. In "weak" cases, axon growth defects are seen only in the dorsal lobes, and the medial lobes appear normal.

Expression of LIMK1Scer\UAS.T:Ivir\HA1 does not cause a detectable wing hair phenotype. Expression of LIMK1Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4ap-md544 at 25oC causes lethality.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, non-suppressible by Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]

Enhancer of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Expression of sshScer\UAS.T:Ivir\HA1 suppresses the axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4ey-OK107. This suppression does not occur in a sickΔ/sickA384 mutant background.

Expression of Rac1Y40C.Scer\UAS.T:Hsap\MYC suppresses the axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4ey-OK107. This suppression does not occur in a sickΔ/sickA384 mutant background.

Expression of sickScer\UAS.T:Disc\RFP-mCherry suppresses the medial axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4ey-OK107. Dorsal axon defects are still observed.

Expression of sickK1881A.Scer\UAS.T:Disc\RFP-mCherry does not suppress the axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4ey-OK107.

The axon growth and increased F-actin phenotypes seen in α/β lobe mushroom body clones expressing LIMK1Scer\UAS.T:Ivir\HA1 are suppressed by sickScer\UAS.T:Disc\RFP-mCherry. ssh1-63 reverses this suppression, with LIMK1Scer\UAS.T:Ivir\HA1 sickScer\UAS.T:Disc\RFP-mCherry ssh1-63 triple mutant clones showing the more severe highly elevated F-actin and 'posterior arrest' (failure of axons to extend to form peduncles and lobe structures) phenotypes seen in LIMK1Scer\UAS.T:Ivir\HA1, ssh1-63 mutants. The phenotype seen in LIMK1Scer\UAS.T:Ivir\HA1, ssh1-63 double mutant clones is more severe than in either single mutant.

Or59c-expressing olfactory receptor neuron clones which are mutant for psidin1 and which are also expressing LIMK1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU show mistargeting in 87% of cases.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
LIMK1Scer\UAS.T:Ivir\HA1
LIMK1UAS.Tag:HA
Name Synonyms
Secondary FlyBase IDs
    References (7)