FB2025_01 , released February 20, 2025
Allele: Dmel\LIMK1UAS.Tag:HA
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General Information
Symbol
Dmel\LIMK1UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0130965
Feature type
allele
Associated gene
Dmel\LIMK1
Associated Insertion(s)
Carried in Construct
P{UAS-LIMK1.HA}
Also Known As
UAS-LIMK1
Key Links
Transgenic product class
wild_type
(Niwa et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Niwa et al., 2002)
Mutagen
in vitro construct
(Niwa et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-LIMK1.HA}
Cytology
Description

UAS sequences drive expression of LIMK1 tagged with Tag:HA.

(Niwa et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
LIMK1
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mushroom bodies expressing LIMK1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey-OK107 display short α/β axonal lobes compared with those of controls, with increased levels of F-actin. Clones expressing LIMK1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey-OK107 also show increased levels of F-actin and both dorsal and medial axon growth defects.

      (Abe et al., 2014)

      Expression of LIMK1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in olfactory receptor neuron (ORN) clones results in 88% of Or59c-expressing ORNs mistargeting.

      (Stephan et al., 2012)

      Expression of LIMK1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4OK107 results in axon growth defects in the mushroom body, with the severity of the phenotype depending on the particular P{UAS-LIMK1.T:Ivir\HA1} insertion line used. In "severe" cases, all mushroom body axon lobes are truncated and there are axon guidance defects at the peduncle, with axons forming ball-like structures in this region. In "strong" cases, no guidance defects are seen, but both the dorsal and medial branches are truncated. In "weak" cases, axon growth defects are seen only in the dorsal lobes, and the medial lobes appear normal.

      (Ng and Luo, 2004)

      Expression of LIMK1Scer\UAS.T:Ivir\HA1 does not cause a detectable wing hair phenotype. Expression of LIMK1Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4ap-md544 at 25oC causes lethality.

      (Niwa et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Statement
      Reference

      BacA\p35UAS.cUa, DroncUAS.Tag:FLAG, LIMK1UAS.Tag:HA, Scer\GAL4ey.PU has increased size | larval stage phenotype, suppressible by Myo31DFHMS00928, Scer\GAL4ey.PU

      (Farrell et al., 2022)

      BacA\p35UAS.cUa, DroncUAS.Tag:FLAG, LIMK1UAS.Tag:HA, Scer\GAL4ey.PU has abnormal size | adult stage phenotype, suppressible by Myo31DFHMS00928, Scer\GAL4ey.PU

      (Farrell et al., 2022)

      BacA\p35UAS.cUa, LIMK1UAS.Tag:HA, Scer\GAL4ey.PU, hidUAS.cUa has abnormal size | adult stage phenotype, suppressible by Myo31DFHMS00928, Scer\GAL4ey.PU

      (Farrell et al., 2022)

      BacA\p35UAS.cUa, LIMK1UAS.Tag:HA, Scer\GAL4ey.PU, hidUAS.cUa has increased size | larval stage phenotype, suppressible by Myo31DFHMS00928, Scer\GAL4ey.PU

      (Farrell et al., 2022)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by NavUAS.mCherry, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy | somatic clone phenotype, suppressible by NavUAS.mCherry, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by Rho172O/Rho1[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by Cdc42[+]/Cdc423

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by Cdc42[+]/Cdc424

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by Pak11/Pak[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by Pak[+]/Pak14

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by Pak[+]/Pak16

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by Rac2Δ/Rac2[+]/Rac1J10/Rac1[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by Rac1J10/Rac1[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by Rho172R/Rho1[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by RhoGEF21.1/RhoGEF2[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by RhoGEF24.1/RhoGEF2[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by dia5

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by pbl[+]/pbl2

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by pbl[+]/pbl3

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by rok[+]/Rok2

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by trio1/trio[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by trio3/trio[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by sifUAS.cSa, Scer\GAL4ey-OK107

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by RhoGAPp190UAS.Tag:MYC, Scer\GAL4ey-OK107

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by tsrS3A.UAS, Scer\GAL4ey-OK107

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has abnormal neuroanatomy phenotype, suppressible by tsrUAS.cNa, Scer\GAL4ey-OK107

      (Ng and Luo, 2004)
      NOT suppressed by
      Enhancer of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      BacA\p35UAS.cUa, DroncUAS.Tag:FLAG, LIMK1UAS.Tag:HA, Scer\GAL4ey.PU has eye disc | larval stage phenotype, suppressible by Myo31DFHMS00928, Scer\GAL4ey.PU

      (Farrell et al., 2022)

      BacA\p35UAS.cUa, DroncUAS.Tag:FLAG, LIMK1UAS.Tag:HA, Scer\GAL4ey.PU has filamentous actin | larval stage phenotype, suppressible by Myo31DFHMS00928, Scer\GAL4ey.PU

      (Farrell et al., 2022)

      BacA\p35UAS.cUa, DroncUAS.Tag:FLAG, LIMK1UAS.Tag:HA, Scer\GAL4ey.PU has adult head phenotype, suppressible by Myo31DFHMS00928, Scer\GAL4ey.PU

      (Farrell et al., 2022)

      BacA\p35UAS.cUa, LIMK1UAS.Tag:HA, Scer\GAL4ey.PU, hidUAS.cUa has adult head phenotype, suppressible by Myo31DFHMS00928, Scer\GAL4ey.PU

      (Farrell et al., 2022)

      BacA\p35UAS.cUa, LIMK1UAS.Tag:HA, Scer\GAL4ey.PU, hidUAS.cUa has eye disc | larval stage phenotype, suppressible by Myo31DFHMS00928, Scer\GAL4ey.PU

      (Farrell et al., 2022)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has mushroom body phenotype, suppressible by NavUAS.mCherry, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body alpha-lobe phenotype, suppressible by NavUAS.mCherry, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body beta-lobe phenotype, suppressible by NavUAS.mCherry, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body alpha-lobe | somatic clone phenotype, suppressible by NavUAS.mCherry, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body beta-lobe | somatic clone phenotype, suppressible by NavUAS.mCherry, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by Rho172O/Rho1[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by Cdc42[+]/Cdc423

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by Cdc42[+]/Cdc424

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by Pak11/Pak[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by Pak[+]/Pak14

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by Pak[+]/Pak16

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by Rac2Δ/Rac2[+]/Rac1J10/Rac1[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by Rac1J10/Rac1[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by Rho172R/Rho1[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by RhoGEF21.1/RhoGEF2[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by RhoGEF24.1/RhoGEF2[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by dia5

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by pbl[+]/pbl2

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by pbl[+]/pbl3

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by rok[+]/Rok2

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by trio1/trio[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by trio3/trio[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by sifUAS.cSa, Scer\GAL4ey-OK107

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by RhoGAPp190UAS.Tag:MYC, Scer\GAL4ey-OK107

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by tsrS3A.UAS, Scer\GAL4ey-OK107

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, suppressible by tsrUAS.cNa, Scer\GAL4ey-OK107

      (Ng and Luo, 2004)
      NOT suppressed by
      Statement
      Reference

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has mushroom body phenotype, non-suppressible by Rac1Y40C.UAS.Tag:MYC/NavA384/NavΔ, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body alpha-lobe phenotype, non-suppressible by Rac1Y40C.UAS.Tag:MYC/NavA384/NavΔ, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body beta-lobe phenotype, non-suppressible by Rac1Y40C.UAS.Tag:MYC/NavA384/NavΔ, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has mushroom body phenotype, non-suppressible by NavK1881A.UAS.mCherry, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body alpha-lobe phenotype, non-suppressible by NavK1881A.UAS.mCherry, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body beta-lobe phenotype, non-suppressible by NavK1881A.UAS.mCherry, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body alpha-lobe | somatic clone phenotype, non-suppressible by NavUAS.mCherry, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body beta-lobe | somatic clone phenotype, non-suppressible by NavUAS.mCherry, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body alpha-lobe phenotype, non-suppressible by sshUAS.Tag:HA/NavA384/NavΔ, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body beta-lobe phenotype, non-suppressible by sshUAS.Tag:HA/NavA384/NavΔ, Scer\GAL4ey-OK107

      (Abe et al., 2014)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, non-suppressible by Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, non-suppressible by SCARΔ37/SCAR[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, non-suppressible by Arpc1Q25sd/Sop2[+]

      (Ng and Luo, 2004)

      LIMK1UAS.Tag:HA, Scer\GAL4ey-OK107 has adult mushroom body phenotype, non-suppressible by tsrS3E.UAS, Scer\GAL4ey-OK107

      (Ng and Luo, 2004)
      Enhancer of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of sshScer\UAS.T:Ivir\HA1 suppresses the axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4ey-OK107. This suppression does not occur in a sickΔ/sickA384 mutant background.

      Expression of Rac1Y40C.Scer\UAS.T:Hsap\MYC suppresses the axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4ey-OK107. This suppression does not occur in a sickΔ/sickA384 mutant background.

      Expression of sickScer\UAS.T:Disc\RFP-mCherry suppresses the medial axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4ey-OK107. Dorsal axon defects are still observed.

      Expression of sickK1881A.Scer\UAS.T:Disc\RFP-mCherry does not suppress the axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4ey-OK107.

      The axon growth and increased F-actin phenotypes seen in α/β lobe mushroom body clones expressing LIMK1Scer\UAS.T:Ivir\HA1 are suppressed by sickScer\UAS.T:Disc\RFP-mCherry. ssh1-63 reverses this suppression, with LIMK1Scer\UAS.T:Ivir\HA1 sickScer\UAS.T:Disc\RFP-mCherry ssh1-63 triple mutant clones showing the more severe highly elevated F-actin and 'posterior arrest' (failure of axons to extend to form peduncles and lobe structures) phenotypes seen in LIMK1Scer\UAS.T:Ivir\HA1, ssh1-63 mutants. The phenotype seen in LIMK1Scer\UAS.T:Ivir\HA1, ssh1-63 double mutant clones is more severe than in either single mutant.

      (Abe et al., 2014)

      Or59c-expressing olfactory receptor neuron clones which are mutant for psidin1 and which are also expressing LIMK1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act.PU show mistargeting in 87% of cases.

      (Stephan et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      LIMK1Scer\UAS.T:Ivir\HA1
      LIMK1UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (10)