Allele Dmel\CanB2^EP774

General Information
Symbol	Dmel\CanB2^EP774	Species	D. melanogaster
Name		FlyBase ID	FBal0131301
Feature type	allele	Associated gene	Dmel\CanB2
Also Known As	canB2^EP(2)0774

Map ( GBrowse )	Untitled Document
Allele class	loss of function allele
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Takeo et al., 2010)
Mutagen	P-element activity (Gene Disruption Project members, 2001-, Gajewski et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2R:3,673,811..3,673,811 (Gene Disruption Project members, 2001-, Gajewski et al., 2003, Gajewski et al., 2006, Takeo et al., 2010, Chang and Min, 2005)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	P{EP}CanB2^EP774 (Gene Disruption Project members, 2001-, Gajewski et al., 2003, Gajewski et al., 2006, Takeo et al., 2010, Chang and Min, 2005)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile \| germline clone (Takeo et al., 2010) flightless, with Scer\GAL4[-]: (Gajewski et al., 2003) meiotic cell cycle defective \| germline clone (Takeo et al., 2010) partially lethal - majority die, with Scer\GAL4[-]: (Gajewski et al., 2003)
Phenotype Manifest In
	dorsal medial muscle, with Scer\GAL4[-]: (Gajewski et al., 2003, Gajewski et al., 2006) dorsal medial muscle (with CanB2¹⁸⁰), with Scer\GAL4[-]: (Gajewski et al., 2003) dorsal medial muscle (with Df(2R)ST1), with Scer\GAL4[-]: (Gajewski et al., 2003) dorsal medial muscle \| P-stage, with Scer\GAL4[-]: (Gajewski et al., 2003) indirect flight muscle, with Scer\GAL4[-]: (Gajewski et al., 2003, Gajewski et al., 2006) indirect flight muscle (with CanB2¹⁸⁰), with Scer\GAL4[-]: (Gajewski et al., 2003) indirect flight muscle (with Df(2R)ST1), with Scer\GAL4[-]: (Gajewski et al., 2003) indirect flight muscle \| P-stage, with Scer\GAL4[-]: (Gajewski et al., 2003) myofibril, with Scer\GAL4[-]: (Gajewski et al., 2006) sarcomere, with Scer\GAL4[-]: (Gajewski et al., 2006) wing, with Scer\GAL4[-]: (Gajewski et al., 2003)
Detailed Description
	Statement Reference Mature oocytes ofCanB2[EP774] germ-line clones appear to have normal metaphase I chromosome configuration. However, the laid eggs fail to hatch. The chromosomes in these eggs exhibit an arrested meiotic anaphase I-like configuration. (Takeo et al., 2010) Over 70% of mid-stage CanB2^EP774 pupae show collapse of all indirect flight muscles. In the remaining 30%, no pupa has more than 7 uncollapsed DLMs (dorsal longitudinal muscles, also known as dorsal medial muscles). CanB2^EP774 indirect flight muscle tissues show a range of phenotypes. In CanB2^EP774 adults, 20% of myofibrils have a wild-type appearance, ~40% exhibit no discernable filament structure in central regions but have some scattered organized filament patches at the periphery. The remaining mutant adults lack myofibrils with any recognizable structures. CanB2^EP774 adult IFMs sometimes have short Z-bands and missing or partial M-lines, indicative of a reduction or absence of thick filaments. CanB2^EP774 pupae show more severe phenotypes than adults, as their IFMs show no organized filament structure and their most severely-affected muscles have no Z-bands or any other sarcomeric structure markers. (Gajewski et al., 2006) Small numbers of CanB2^EP774 animals survive to adulthood. These animals are flightless, with wings positioned at abnormal angles. In CanB2^EP774 homozygous, CanB2^EP774/CanB2¹⁸⁰ and CanB2^EP774/Df(2R)ST1 animals, large gaps or deletions occur in the centrally located dorsal medial muscle pairs. In most cases, one or more dorsal muscles are present, and on rare occasions, one or more ventral muscles. However, the dorsal ventral and trochanter muscle pairs are intact and normally arranged in these flies. In contrast to the precisely arrayed indirect flight muscles present in posterior thoraces of wild-type animals, the posterior thoraces of CanB2^EP774 homozygous, CanB2^EP774/CanB2¹⁸⁰ and CanB2^EP774/Df(2R)ST1 animals contain large, poorly defined muscle masses. Indirect flight muscle formation in CanB2^EP774 homozygotes is normal in day 2 pupae. During pupal day 3 there is a slow, progressive retraction of the indirect flight muscles towards the posterior of the thorax after they initiate observable muscle contractions. By day 4 all dorsal medial muscle and dorsal ventral muscles are mis-located in a posterior thoracic position. (Gajewski et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference CanB2^EP774/CanB2[+], Scer\GAL4^how-24B/Scer\GAL4^how-24B is a suppressor of lethal \| pupal stage phenotype of Mmus\Ppp3ca^Act.Scer\UAS, Scer\GAL4^how-24B/Scer\GAL4^how-24B (Gajewski et al., 2003) CanB2^EP774/CanB2[+] is a suppressor \| partially of female sterile phenotype of Pp2B-14D^act.Scer\UAS, Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Takeo et al., 2010)
Phenotype Manifest In
Suppressed by
Statement Reference CanB2^EP774, Scer\GAL4[-] has dorsal medial muscle phenotype, suppressible \| partially by fliI³/fliI³ (Gajewski et al., 2006)
Enhancer of
Statement Reference CanB2^EP774/CanB2[+] is an enhancer of indirect flight muscle phenotype of Mhc⁵ (Gajewski et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference CanB2[EP774]/+ partially suppresses the female sterility associated with the overexpression of Pp2B-14D[act.Scer\UAS] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16]. (Takeo et al., 2010) fliI³; CanB2^EP774 flies show a strong suppression of the indirect flight muscle collapse phenotype of CanB2^EP774 single mutants. The majority of the DLM fail to collapse in the double mutants; all double mutants have at least 7 DLMs present, with some mutants exhibiting as many as 12 DLMs. However, these DLMs have smaller fibers than in wild type and often exhibit holes in the cytoplasm. Mhc⁵/+, CanB2^EP774/+ double mutants show an increase in the level of severely-defective indirect flight muscles compared to Mhc⁵/+ single mutants (from 55 to 80%). (Gajewski et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	CanB2^EP774 is partially rescued by Scer\GAL4^{nos.UTR.T:Hsim\VP16}/CanB2^{Scer\UAS.P\T.cTa} (Takeo et al., 2010)
Comments	The meiotic phenotype in eggs resulting from CanB2[EP774] mutant germ-line clones is partially rescued by the expression of CanB2[Scer\UAS.P\T.cTa] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16]. (Takeo et al., 2010)
Stocks ( 1 )
Bloomington	17296 w¹¹¹⁸; P{EP}CanB2^EP774
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CanB2^EP774 canB2^EP(2)0774 (Gajewski et al., 2006) CanB2^EP(2)0774 (Takeo et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Takeo et al., 2010, Dev. Biol. 344(2): 957--967 Calcineurin and its regulation by Sra/RCAN is required for completion of meiosis in Drosophila. [FBrf0211392] Gajewski et al., 2006, Dev. Biol. 289(1): 17--29 Calcineurin function is required for myofilament formation and troponin I isoform transition in Drosophila indirect flight muscle. [FBrf0190120] Chang and Min, 2005, Nat. Neurosci. 8(11): 1577--1585 Drosophila melanogaster homolog of Down syndrome critical region 1 is critical for mitochondrial function. [FBrf0191278] Gajewski et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 100(3): 1040--1045 Requirement of the calcineurin subunit gene canB2 for indirect flight muscle formation in Drosophila. [FBrf0156033]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]

Allele Dmel\CanB2EP774

Allele Dmel\CanB2^EP774