FlyBase Allele Report: Dmel\CSN5[quo-1]

General Information

Symbol

Dmel\CSN5^quo-1

Species

D. melanogaster

Name

FlyBase ID

FBal0131314

Feature type

allele

Associated gene

Dmel\CSN5

Associated Insertion(s)

Carried in Construct

Also Known As

CSN5¹

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Suh et al., 2002)

Mutagen

ethyl methanesulfonate

(Suh et al., 2002)

Progenitor genotype

Cytology

Description

Amino acid replacement: E160V.

(Oron et al., 2002, Suh et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:16,470,710..16,470,710 [+]

Nucleotide change:

A16470710T

Amino acid change:

E160V | CSN5-PA; E160V | CSN5-PB

Reported amino acid change:

E160V

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Oron et al., 2002, Suh et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Gemmill et al., 2005)

lethal - all die before end of pupal stage (with CSN5^null)

(Gemmill et al., 2005)

lethal - all die before end of pupal stage | recessive

(Suh et al., 2002)

some die during pupal stage (with CSN5^null)

(Gemmill et al., 2005)

some die during pupal stage | recessive

(Suh et al., 2002)

Phenotype Manifest In

lamina anlage glial cell

(Suh et al., 2002)

lamina anlage glial cell | somatic clone

(Suh et al., 2002)

ovary | germline clone

(Oron et al., 2002)

photoreceptor cell R1 & axon | somatic clone

(Suh et al., 2002)

photoreceptor cell R2 & axon

(Suh et al., 2002)

photoreceptor cell R2 & axon (with Df(3R)RK6-3)

(Suh et al., 2002)

photoreceptor cell R2 & axon | somatic clone

(Suh et al., 2002)

photoreceptor cell R3 & axon

(Suh et al., 2002)

photoreceptor cell R3 & axon (with Df(3R)RK6-3)

(Suh et al., 2002)

photoreceptor cell R3 & axon | somatic clone

(Suh et al., 2002)

photoreceptor cell R4 & axon

(Suh et al., 2002)

photoreceptor cell R4 & axon (with Df(3R)RK6-3)

(Suh et al., 2002)

photoreceptor cell R4 & axon | somatic clone

(Suh et al., 2002)

photoreceptor cell R5 & axon

(Suh et al., 2002)

photoreceptor cell R5 & axon (with Df(3R)RK6-3)

(Suh et al., 2002)

photoreceptor cell R5 & axon | somatic clone

(Suh et al., 2002)

photoreceptor cell R6 & axon | somatic clone

(Suh et al., 2002)

Detailed Description

Statement

Reference

Oogenesis is arrested at stage 5-6 in females containing homozygous germline clones.

(Oron et al., 2002)

Homozygotes die as larvae or pupae. In mutant larvae (including CSN5^quo-1/Df(3R)RK6-3 animals) many R2-R5 axons fail to terminate in the lamina (as seen in wild-type) and instead, project into the medulla. This phenotype is not due to a transformation of R2-R5 into R7 and/or R8. When homozygous CSN5^quo-1 somatic clones R1-R6 mistargeting phenotypes are seen. Laminal glial cell development is disrupted in the developing mutant larval eye, in CSN5^quo-1 homozygotes and mosaic animals in which the eye is mutant and the target wild-type there is a marked reduction in lamina glial cell number (approximately 46% of wild-type) and the remaining cells form disorganised rows. The phenotype is consistent with a defect in laminal glial cell migration.

(Suh et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

CSN5^quo-1/CSN5[+] is a non-suppressor of abnormal size phenotype of Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC

(Gemmill et al., 2005)

Other

Statement

Reference

CSN5^quo-1/CSN5[+], Scer\GAL4^en.PU, Trc8^UAS.Tag:MYC has lethal phenotype

(Gemmill et al., 2005)

CSN5^quo-1, ago⁴/ago[+] has increased cell death | oogenesis phenotype

(Doronkin et al., 2003)

CSN5^quo-1, ago⁴/ago[+] has abnormal cell number | oogenesis phenotype

(Doronkin et al., 2003)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

CSN5^quo-1/CSN5[+] is a non-suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, Trc8^UAS.Tag:MYC

(Gemmill et al., 2005)

Other

Statement

Reference

CSN5^quo-1, ago⁴ has nurse cell phenotype

(Doronkin et al., 2003)

CSN5^quo-1, ago⁴ has nurse cell & nucleus phenotype

(Doronkin et al., 2003)

CSN5^quo-1, ago⁴/ago[+] has nurse cell phenotype

(Doronkin et al., 2003)

CSN5^quo-1, ago⁴/ago[+] has nurse cell & nucleus phenotype

(Doronkin et al., 2003)

CSN5^quo-1/CSN5[+], ago⁴ has nurse cell phenotype

(Doronkin et al., 2003)

CSN5^quo-1/CSN5[+], ago⁴ has nurse cell & nucleus phenotype

(Doronkin et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Unlike either single heterozygote, ovaries from CSN5^quo-1; ago⁴ transheterozygous females exhibit ectopic cell death and increased or decreased nurse cells per egg chamber. Decreased nurse cell numbers per cyst are accompanied by an increase in nurse cell ploidy, suggesting that the decrease results from a block in cell division without a block in DNA replication. In addition, strongly DAPI staining regions of nurse cell nuclei (which may respond to compact heterochromatin) are enlarged compared to wild-type.

(Doronkin et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

CSN5^quo-1 is rescued by CSN5^UAS.cSa/Scer\GAL4^elav.PLu

(Suh et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CSN5¹

(Gemmill et al., 2005, Suh et al., 2002)

CSN5^quo-1

csn5¹

(Oron et al., 2002)

quo¹

(Suh et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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