FlyBase Allele Report: Dmel\Lsd-2[KG00149]

General Information

Symbol

Dmel\Lsd-2^KG00149

Species

D. melanogaster

Name

FlyBase ID

FBal0131346

Feature type

allele

Associated gene

Dmel\Lsd-2

Associated Insertion(s)

P{SUPor-P}Lsd-2^KG00149

Carried in Construct

Also Known As

Lsd-2^KG, Lsd2^KG00149, plin2^KG00149

Genomic Maps

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Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Welte et al., 2005)

Mutagen

P-element activity

(Gronke, 2003, Gronke et al., 2003, Teixeira et al., 2003, Gene Disruption Project members, 2001-)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

transposable element insertion site

X:15,075,975..15,075,975 [+]

(Zhang et al., 2011, Welte et al., 2005, Gronke, 2003, Gronke et al., 2003, Teixeira et al., 2003, Gene Disruption Project members, 2001-)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

The P{SUPor-P} insertion is within the 5' untranslated leader sequence of Lsd-2.

(Gronke et al., 2003)

P{SUPor-P} insertion in the 5' UTR.

(Teixeira et al., 2003)

Insertion components

P{SUPor-P}Lsd-2^KG00149

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates obesity

modeled by HDAC6^KO

(Yan et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

fertile

(Welte et al., 2005)

viable

(Welte et al., 2005)

Phenotype Manifest In

embryonic/larval fat body | larval stage

(Bi et al., 2012)

fat body

(Gronke et al., 2003)

lipid droplet

(Bi et al., 2012)

lipid droplet | embryonic stage | maternal effect

(Welte et al., 2005)

nurse cell (with Lsd-2¹)

(Teixeira et al., 2003)

Detailed Description

Statement

Reference

Lsd-2^KG00149 knockdown leads to a reduction in phase IIa inward shift of lipid droplets.

(Arora et al., 2016)

Lipid droplets in the larval fat body are smaller than normal in mutant animals. The total glyceride level is reduced compared to wild type.

(Bi et al., 2012)

Lsd-2^KG00149 mutants have no ectopic lipid storage in salivary glands.

(Tian et al., 2011)

The net plus-end (phase II) transport of lipid droplets along microtubules is disrupted in Lsd-2^KG00149 embryos; lipid droplets are distributed throughout the periphery in the mutants during this phase, while droplets are localized basally in wild-type embryos. Quantification of droplet motion shows that both plus-end and minus-end periods of uninterrupted motion are slightly decreased in relation to the wild type in phase II, but the decrease in plus-end motion is larger, resulting in abolishment of net transport. For both directions, motion is slightly slower in the mutants than in wild-type embryos. The parameters of droplet motion in phase II and phase III (net minus-end transport) are essentially the same in Lsd-2^KG00149 embryos. Lsd-2^KG00149/+ embryos only show this phenotype when the Lsd-2^KG00149 allele is inherited from the mother. Embryonic development does not appear to be affected in Lsd-2^KG00149 mutants and the distribution of other organelles is not affected. Many embryos hatch and give rise to fertile adults.

(Welte et al., 2005)

Mutants are lean, containing significantly lower levels of triacylglycerols (37.2% of controls).

(Gronke et al., 2003)

Lsd-2¹/Lsd-2^KG00149 nurse cells show an abnormal pattern of neutral lipid accumulation.

(Teixeira et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Lsd-2^KG00149 has lipid droplet phenotype, enhanceable by Lsd-1³⁸

(Bi et al., 2012)

Lsd-2^KG00149 has embryonic/larval fat body | larval stage phenotype, enhanceable by Lsd-1³⁸

(Bi et al., 2012)

Suppressed by

Statement

Reference

Lsd-2^KG00149 has lipid droplet phenotype, suppressible | partially by Lsd-1^UAS.cBa/Scer\GAL4^ppl.PP

(Bi et al., 2012)

Lsd-2^KG00149 has embryonic/larval fat body | larval stage phenotype, suppressible | partially by Lsd-1^UAS.cBa/Scer\GAL4^ppl.PP

(Bi et al., 2012)

NOT Enhancer of

Statement

Reference

Lsd-2^KG00149 is a non-enhancer of fat body phenotype of Seipin^null

(Tian et al., 2011)

Lsd-2^KG00149 is a non-enhancer of lipid droplet phenotype of Seipin^null

(Tian et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Df(2L)Δhalo^AJ/Df(2L)Δhalo^AJ embryos with Lsd-2^KG00149 knockdown display droplet accumulation below the nuclei, thus droplets are shifted outward (compared to position in embryos with Lsd-2^KG00149 knockdown alone.

(Arora et al., 2016)

Expression of Lsd-1^Scer\UAS.cBa under the control of Scer\GAL4^ppl.PP mildly suppresses the small lipid droplet phenotype seen in Lsd-2^KG00149 larvae.

The reduction in size of the lipid droplets and the reduction in total glyceride level is even more severe in Lsd-1³⁸ Lsd-2^KG00149 double mutant larvae than in Lsd-2^KG00149 single mutants.

(Bi et al., 2012)

Fat body lipid storage in Seipin^null; Lsd-2^KG00149 animals is similar to Seipin^null single mutants.

(Tian et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Lsd-2^KG00149 is rescued by Scer\GAL4^fat/Lsd-2^UAS.cGa

(Gronke et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

13382

y¹ P{SUPor-P}Lsd-2^KG00149; ry⁵⁰⁶

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG9057^KG00149

Lsd-2^KG00149

(Tian et al., 2011, Sieber and Thummel, 2009)

Lsd-2^KG

(Sieber and Spradling, 2015, Welte et al., 2005)

Lsd2^KG00149

(Teixeira et al., 2003)

Lsd2^KG

(Zhang et al., 2011)

Plin2^KG00149

(Yan et al., 2017)

plin2^KG00149

(Bi et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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