The P{SUPor-P} insertion is within the 5' untranslated leader sequence of Lsd-2.
P{SUPor-P} insertion in the 5' UTR.
Lsd-2KG00149 knockdown leads to a reduction in phase IIa inward shift of lipid droplets.
Lipid droplets in the larval fat body are smaller than normal in mutant animals. The total glyceride level is reduced compared to wild type.
Lsd-2KG00149 mutants have no ectopic lipid storage in salivary glands.
The net plus-end (phase II) transport of lipid droplets along microtubules is disrupted in Lsd-2KG00149 embryos; lipid droplets are distributed throughout the periphery in the mutants during this phase, while droplets are localized basally in wild-type embryos. Quantification of droplet motion shows that both plus-end and minus-end periods of uninterrupted motion are slightly decreased in relation to the wild type in phase II, but the decrease in plus-end motion is larger, resulting in abolishment of net transport. For both directions, motion is slightly slower in the mutants than in wild-type embryos. The parameters of droplet motion in phase II and phase III (net minus-end transport) are essentially the same in Lsd-2KG00149 embryos. Lsd-2KG00149/+ embryos only show this phenotype when the Lsd-2KG00149 allele is inherited from the mother. Embryonic development does not appear to be affected in Lsd-2KG00149 mutants and the distribution of other organelles is not affected. Many embryos hatch and give rise to fertile adults.
Mutants are lean, containing significantly lower levels of triacylglycerols (37.2% of controls).
Lsd-21/Lsd-2KG00149 nurse cells show an abnormal pattern of neutral lipid accumulation.
Lsd-2KG00149 has lipid droplet phenotype, enhanceable by Lsd-138
Lsd-2KG00149 has embryonic/larval fat body | larval stage phenotype, enhanceable by Lsd-138
Lsd-2KG00149 has lipid droplet phenotype, suppressible | partially by Lsd-1UAS.cBa/Scer\GAL4ppl.PP
Lsd-2KG00149 has embryonic/larval fat body | larval stage phenotype, suppressible | partially by Lsd-1UAS.cBa/Scer\GAL4ppl.PP
Lsd-2KG00149 is a non-enhancer of fat body phenotype of Seipinnull
Lsd-2KG00149 is a non-enhancer of lipid droplet phenotype of Seipinnull
Df(2L)ΔhaloAJ/Df(2L)ΔhaloAJ embryos with Lsd-2KG00149 knockdown display droplet accumulation below the nuclei, thus droplets are shifted outward (compared to position in embryos with Lsd-2KG00149 knockdown alone.
Expression of Lsd-1Scer\UAS.cBa under the control of Scer\GAL4ppl.PP mildly suppresses the small lipid droplet phenotype seen in Lsd-2KG00149 larvae.
The reduction in size of the lipid droplets and the reduction in total glyceride level is even more severe in Lsd-138 Lsd-2KG00149 double mutant larvae than in Lsd-2KG00149 single mutants.
Fat body lipid storage in Seipinnull; Lsd-2KG00149 animals is similar to Seipinnull single mutants.
Lsd-2KG00149 is rescued by Scer\GAL4fat/Lsd-2UAS.cGa