FlyBase Allele Report: Dmel\sea[EP3364]

General Information

Symbol

Dmel\sea^EP3364

Species

D. melanogaster

Name

FlyBase ID

FBal0131420

Feature type

allele

Associated gene

Dmel\sea

Associated Insertion(s)

P{EP}sea^EP3364

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Morciano et al., 2009)

Mutagen

P-element activity

(FlyBase Genome Annotators, 2002-2003, Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EP}sea^EP3364

Cytology

Description

Insertion 437bp upstream of the putative start codon.

(Morciano et al., 2009)

The P{EP}sea^EP3364 insertion maps within a dicistronic processed transcript that encodes two non-overlapping open reading frames (fabp and sea).

(FlyBase Genome Annotators, 2002-2003)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:11,561,612..11,561,612 [+]

(Tiwari et al., 2020, Morciano et al., 2009, FlyBase Genome Annotators, 2002-2003, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Morciano et al., 2009)

abnormal mitotic cell cycle (with Df(3R)Mx-1)

(Morciano et al., 2009)

abnormal mitotic cell cycle (with sea^Δ24)

(Morciano et al., 2009)

Phenotype Manifest In

chromosome | larval stage

(Morciano et al., 2019)

condensed chromosome

(Morciano et al., 2009)

condensed chromosome (with Df(3R)Mx-1)

(Morciano et al., 2009)

condensed chromosome (with sea^Δ24)

(Morciano et al., 2009)

larval neuroblast

(Morciano et al., 2009)

larval neuroblast (with Df(3R)Mx-1)

(Morciano et al., 2009)

larval neuroblast (with sea^Δ24)

(Morciano et al., 2009)

larval neuroblast | larval stage (with Df(2R)Exel7138)

(Morciano et al., 2019)

Detailed Description

Statement

Reference

sea^EP3364 homozygous larval neuroblasts show a high frequency of chromosome breaks, which are almost absent in wild-type controls.

(Morciano et al., 2019)

Brain neuroblasts of homozygous and sea^EP3364/Df(3R)Mx-1 larvae show defects in mitosis; approximately 40% have chromatid and chromosome breaks, and approximately 3% show chromosome exchanges. In addition, approximately 10% of the mutant cells show a more complex breakage phenotype consisting of multiple chromosome splinters. The mutant neuroblasts show an approximately 3-fold reduction in the mitotic index compared to controls, although the frequency of anaphases is indistinguishable from wild type.

Brain neuroblasts of sea^Δ24/sea^EP3364 larvae show defects in mitosis, with a high frequency of chromosome breaks. Mutant cells with highly fragmented nuclei containing chromosome "splinters" are also seen.

(Morciano et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

sea^EP3364 has FlyBase_internalproperty type:chromosome:chromosome | larval stage phenotype, enhanceable by Df(2R)Exel7138/Acly⁰¹⁴⁶⁶

(Morciano et al., 2019)

sea^EP3364/Df(2R)Exel7138 has larval neuroblast | larval stage phenotype, enhanceable by Df(2R)Exel7138/Acly⁰¹⁴⁶⁶

(Morciano et al., 2019)

Enhancer of

Statement

Reference

sea^EP3364/sea^EP3364 is an enhancer of FlyBase_internalproperty type:chromosome:chromosome | larval stage phenotype of Acly⁰¹⁴⁶⁶

(Morciano et al., 2019)

sea^EP3364/sea^EP3364 is an enhancer of larval neuroblast | larval stage phenotype of Df(2R)Exel7138/Acly⁰¹⁴⁶⁶

(Morciano et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

whd¹/+ sea^EP3364/+ exhibit a decline in the cortical zone of lymph gland primary lobe of larvae when compared to controls.

(Tiwari et al., 2020)

rad50^Δ5.1 does not override the reduced mitotic index of sea^EP3364 single mutants.

mre11^unspecified does not override the reduced mitotic index of sea^EP3364 single mutants.

(Morciano et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sea^EP3364 is rescued by sea^hs.PM

(Morciano et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

17118

w¹¹¹⁸; P{EP}sea^EP3364 fabp^EP3364/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG6782^EP3364

l(3)EP3364

(Morciano et al., 2009)

sea^EP3364

(Tiwari et al., 2020, Morciano et al., 2019, Grice et al., 2015)

sea^EP

(Morciano et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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