Drp1KG03815 clones in fat bodies from well-fed early third instar larvae accumulate lysosomes, as indicated by elevated LysoTracker staining, and show redistribution of the Atg8a product into autophagosomes.
Primary spermatocytes in homozygous germline clones induced in males contain abnormally clustered mitochondria. 52% of early round spermatids derived from these spermatocytes retain mitochondrial material and in all cases the mitochondria appear not to have separated properly during meiosis, remaining connected and appearing to pass through the cytoplasmic bridges between spermatids. The remaining 48% of the mutant spermatids have little or no mitochondrial material. At later stages of flagellar elongation, the mutant spermatids have defects in unfurling and elongation of mitochondria, with the spermatids containing spaghetti-like interconnected masses of mitochondria that fail to elongate properly and are associated with multiple nuclei. The ratio of the observed mitochondrial masses to nuclei is 8:34 , consistent with each mass typically representing the mitochondrial material from all four meiotic products of a single spermatocyte.