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General Information
Symbol
Dmel\Drp1KG03815
Species
D. melanogaster
Name
FlyBase ID
FBal0131515
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
KG03815
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{SUPor-P}Drp1KG03815
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

autophagic vacuole & larval fat body | somatic clone | cell autonomous

lysosome & larval fat body | somatic clone | cell autonomous

mitochondrion & primary spermatocyte | germ-line clone

Detailed Description
Statement
Reference

Drp1KG03815 clones in fat bodies from well-fed early third instar larvae accumulate lysosomes, as indicated by elevated LysoTracker staining, and show redistribution of the Atg8a product into autophagosomes.

Drp1KG03815/+ mutants are fully viable.

Primary spermatocytes in homozygous germline clones induced in males contain abnormally clustered mitochondria. 52% of early round spermatids derived from these spermatocytes retain mitochondrial material and in all cases the mitochondria appear not to have separated properly during meiosis, remaining connected and appearing to pass through the cytoplasmic bridges between spermatids. The remaining 48% of the mutant spermatids have little or no mitochondrial material. At later stages of flagellar elongation, the mutant spermatids have defects in unfurling and elongation of mitochondria, with the spermatids containing spaghetti-like interconnected masses of mitochondria that fail to elongate properly and are associated with multiple nuclei. The ratio of the observed mitochondrial masses to nuclei is 8:34 , consistent with each mass typically representing the mitochondrial material from all four meiotic products of a single spermatocyte.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Drp1KG03815/+ in combination with park25/park25 mutants results in lethality. Viability of flies Drp1KG03815/+ is unaffected in a park25/+ background.

Drp1KG03815/+ in combination with Pink1B9 hemizygous mutations results in almost complete lethality. The few surviving adults are developmentally delayed and are substantially smaller and short lived than single Pink1B9 hemizygous mutants. Viability of Drp1KG03815/+ flies is unaffected in a heterozygous Pink1B9/+ background.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Precise excision of the P{SUPor-P} element reverts the lethal phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (10)