|Feature type||allele||Associated gene||Dmel\mars|
|Map ( GBrowse )||
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Caused by insertion|
|Phenotype Manifest In|
The hatching rate of embryos from parents homozygous for mars[EP2477] is 67%. Viability is also temperature-dependent and the hatching rate is further reduced to 39.2% at 28.5[o]C. Gynandromorphs form approximately 0.05% of the progeny from mars[EP2477] parents. In metaphase mars[EP2477] embryos, the length and the density of mitotic spindles is significantly shorter and less, respectively, than in controls. At anaphase and at telophase, neither the length nor the density of mitotic spindles are substantially affected, indicating that polymerisation of the mitotic spindles in mars[EP2477] embryos is delayed, but not entirely prevented. Consistent with this, progression of mitotic events in patches of nuclei, each of which contain at leat two nuclei, is obviously delayed compared to those in adjacent nuclei in 13% of mars[EP2477] embryos. The frequencies of non-disjunction in male progeny from mars[EP2477] homozygotes at 24[o]C and 28.5[o]C were 0.6% and 1.1% respectively, which are much higher than the gynandromorphy rate in the mars parent. mars[EP2477] overexpression in the eye disc under the control of Scer\GAL4[GMR.PF] results in cells in domain I being retained at interphase and many cells in domains II and III being retained at M-phase. This should be compared to the fact that most M-phase cells appear in domain I in control eye discs.
Eggs laid by mars[EP2477] and mars/mars[EP2477] flies show a greatly reduced ability to hatch. Sperm tails can be visualised in early-arrested embryos laid by mutant females, indicating that sterility is caused by arrest in embryogenesis after fertilization. Approximately 51% of embryos laid by mars[EP2477] mothers fail to develop beyond embryogenesis. Many show terminal phenotypes at or shortly after gastrulation.
marsEP2477 has mitotic cell cycle defective | temperature conditional phenotype, enhanceable by tlk[+]/tlk27-9
marsEP2477 has mitotic cell cycle defective | temperature conditional phenotype, enhanceable by tlk[+]/tlkΔ14
mars1/marsEP2477 has lethal | embryonic stage phenotype, suppressible by taccS863L.Ubi-p63E.T:Avic\GFP
|NOT suppressed by|
marsEP2477 is an enhancer of mitotic cell cycle defective | temperature conditional phenotype of tlk27-9
|Phenotype Manifest In|
The hatching rate from tlk[27-9]/+; mars[EP2477] females is 48%, a further reduction from the 67% for mars[EP2477] embryos, indicating that mars genetically interacts with tlk. The frequencies of non-disjunction in male progenies from tlk[27-9]/+; mars[EP2477] and tlk[Δ14]/+; mars[EP2477] females at 28.5[o]C is 6.1% and 4.4%; these rates are much higher than those from mars[EP2477] (1.1%) or tlk[Δ14]/+ females (0.4%). This increase in frequency of non-disjunction is also temperature dependent, since there is no substantial increases in progeny from tlk[27-9]/+; mars[EP2477] and tlk[Δ14]/+; mars[EP2477] females at 24[o]C when compared to mars[EP2477] or tlk[Δ14]/+ females. All tlk[Δ14]/+; mars[EP2477] embryos at either metaphase or anaphase exhibit asynchrony so severe that it is impossible to distinguish what phase an embryo belongs to. Asynchronous chromosome segregation is observed in embryos where most of the nuclei are likely to be at anaphase. Despite this severe asynchrony during chromosome congression or segregation, the morphology of the mitotic spindles in tlk[Δ14]/+; mars[EP2477] embryos is not significantly different from that in mars[EP2477] embryos. Co-expression of mars[EP2477] and tlk[Scer\UAS.cCa] in the eye disc under the control of Scer\GAL4[GMR.PF] results in the number of M-phase cells in domain III being reduced to a level close to that of wild-type, showing that metaphase arrest induced by mars[EP2477] overexpression is suppressed by tlk[Scer\UAS.cCa] overexpression.
|Complementation & Rescue Data|
|Not rescued by|
Expression of mars[Scer\UAS.P\T.cTa] in the female germline, under the control of Scer\GAL4[arm.PS] restores embryo viability and fertility in mars/mars[EP2477] and mars[EP2477] mutants. Ectopic expression of mars[F839A.Scer\UAS.P\T] under the control of Scer\GAL4[arm.PS] fails to rescue the embryonic lethality of embryos laid by mars/mars[EP2477] mothers.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
|Secondary FlyBase IDs|
|References ( 5 )|
|Personal communication to FlyBase|