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General Information
Symbol
Dmel\Atg1EP3348
Species
D. melanogaster
Name
FlyBase ID
FBal0131706
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
EP(3)3348, EP3348
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion in the first exon, 5bp downstream of the first ATG codon.

Insertion within the first exon, 539bp upstream of the ATG start codon.

P{EP} is inserted into the 5' UTR of Atg1, 541bp upstream of the start of the Atg1 coding region.

Insertion components
P{EP}Atg1EP3348
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Heterozygous embryos have distorted and broken longitudinal connectives.

Homozygous embryos show increased distortion of the commissures and longitudinal connectives.

Expression using Scer\GAL4ey.PU does not induce a rough eye phenotype.

About 30% of Atg1EP3348 homozygous mutants develop into adults. No mitotic defects are evident in the larval brain and imaginal discs of mutant animals.

Atg1EP3348 mutant animals show marked defects in the induction of autophagy and autophagosome formation under conditions of starvation.

Mutants show disruption of autophagy.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Phenotype Manifest In
NOT suppressed by
Statement
Reference

Atg1EP3348 has autophagosome | conditional phenotype, non-suppressible by S6k[+]/S6k07084

Atg1EP3348 has autophagosome | conditional phenotype, non-suppressible by S6k[+]/S6kl-1

Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference

Atg1[+]/Atg1EP3348 is a suppressor of fat body | second instar larval stage phenotype of Tork17004

Atg1[+]/Atg1EP3348 is a suppressor of vesicle phenotype of Tork17004

Atg1[+]/Atg1EP3348 is a suppressor | partially of nucleus phenotype of Tork17004

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Defects in autophagosome formation in starved Atg1EP3348 homozygous larvae are not rescued in a S6k07084 or S6kl-1 heterozygous background. However, eclosion rates are increased in these animals in a dose-dependent manner. Roughly 50% of Atg1EP3348, S6k07084/+ eclose, increased to 70% in a Atg1EP3348, S6kl-1/+ genetic background.

Tork17004 homozygous animals in a heterozygous Atg1EP3348 genetic background grow faster, and extend their developmental stage to mid/late third instar larval stage. Double homozygous animals survive to mid-late third instar larval stage.

Lipid vesicle aggregation in the fat body of Tork17004 homozygous second/early third instar larvae is suppressed in a heterozygous Atg1EP3348 genetic background.

Tork17004 mutant larvae heterozygous for Atg1EP3348 show a partially rescued cell and nuclear salivary gland size.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Precise excisions of the P{EP} result in viability.

Precise excision of the insertion rescues the semi-lethality.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
CG10967EP3348
l(3)00305EP3348
Name Synonyms
Secondary FlyBase IDs
    References (9)