FB2025_01 , released February 20, 2025
Allele: Dmel\witUAS.cMa
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General Information
Symbol
Dmel\witUAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Marques
FlyBase ID
FBal0133961
Feature type
allele
Associated gene
Dmel\wit
Associated Insertion(s)
Carried in Construct
P{UAS-wit.M}
Also Known As
UAS-wit
Key Links
Transgenic product class
cDNA
(Marques et al., 2002)
wild_type
(Marques et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Marques et al., 2002)
wild_type
(Marques et al., 2002)
Mutagen
in vitro construct
(Aberle et al., 2002, Marques et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-wit.M}
Cytology
Description

A 4.3kb HindIII/NotI fragment containing a complete wit cDNA is driven by UASt sequences.

(Marques et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
wit
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  childhood spinal muscular atrophy
      modeled by Smn73Ao
      (Chang et al., 2008)
      modeled by Smnf01109
      (Chang et al., 2008)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of witScer\UAS.cMa under the control of Scer\GAL4elav.PU does not lead to significant changes in the number of synaptic boutons at the third instar larval neuromuscular junction, as compared to controls.

      (Banerjee et al., 2017)

      Expression of witScer\UAS.cMa under the control of Scer\GAL4elav.PLu has no obvious effect on bouton number per muscle are at the third instar larval neuromuscular junction.

      (Chang et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      witUAS.cMa/Scer\GAL4elav.PLu is a suppressor of bouton phenotype of ImpĪ²1170, wit[+]/witHA1

      (Higashi-Kovtun et al., 2010)
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The expression of witScer\UAS.cMa under the control of Scer\GAL4elav.PU does not suppress the decreased number of synaptic boutons at the third instar larval neuromuscular of Nrx-1273 homozygotes.

      (Banerjee et al., 2017)

      Neuronal expression of witScer\UAS.cMa (under the control of Scer\GAL4elav.PLu reverses the reduction in bouton number in Ranbp1170/+ ; witHA1/+ larvae.

      (Higashi-Kovtun et al., 2010)

      Expression of witScer\UAS.cMa under the control of Scer\GAL4elav.PLu in a Smnf01109/+ or Smn73Ao/+ background results in a reduction in bouton number per muscle area at the third larval instar neuromuscular junction compared to wild type.

      (Chang et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav-C155/witUAS.cMa rescues witHA2

      (Aberle et al., 2002)

      Scer\GAL4RapGAP1-OK6/witUAS.cMa rescues witHA2

      (Aberle et al., 2002)

      Scer\GAL4elav-C155/witUAS.cMa rescues witHA2/witHA4

      (Aberle et al., 2002)

      Scer\GAL4BG380/witUAS.cMa rescues witunspecified

      (Marques et al., 2002)

      Scer\GAL4RapGAP1-OK6/witUAS.cMa rescues witunspecified

      (Marques et al., 2002)

      Scer\GAL4elav-C155/witUAS.cMa rescues witunspecified

      (Marques et al., 2002)

      witUAS.cMa/Scer\GAL4nrv2.PS rescues witunspecified

      (Marques et al., 2002)
      Partially rescues

      Scer\GAL4CCAP.PP/witUAS.cMa partially rescues witB11/witA12

      (Veverytsa and Allan, 2011)

      Scer\GAL4elav-C155/witUAS.cMa partially rescues witA12/witC175

      (Marques et al., 2002)
      Fails to rescue

      Scer\GAL4Mhc.PW/witUAS.cMa fails to rescue witunspecified

      (Marques et al., 2002)

      Scer\GAL4twi.2PE/witUAS.cMa fails to rescue witunspecified

      (Marques et al., 2002)
      Comments

      The addition of witScer\UAS.cMa driven by Scer\GAL4elav-C155 rescues the lethality and muscular growth defects seen in witHA2 homozygotes or witHA2/witHA4 mutants. The addition of witScer\UAS.cMa driven by Scer\GAL4OK6 rescues the lethality and synaptic defects seen in witHA2 homozygotes.

      (Aberle et al., 2002)

      The addition of witScer\UAS.cMa driven by Scer\GAL4elav-C155 to witA12/witC175 animals partially rescues the EJC amplitude reduction phenotype seen in mutants.

      (Marques et al., 2002)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      witScer\UAS.cMa
      witUAS.cMa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Marques
      (Aberle et al., 2002, Marques et al., 2002)
      Secondary FlyBase IDs
        References (10)