Allele Dmel\wit^HA3

General Information
Symbol	Dmel\wit^HA3	Species	D. melanogaster
Name		FlyBase ID	FBal0133965
Feature type	allele	Associated gene	Dmel\wit

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Aberle et al., 2002)
Mutagen	ethyl methanesulfonate (Aberle et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:4,066,546..4,066,546 comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G4066546A pr_change=W491@\|wit-PA reported_pr_change=W491@ (Aberle et al., 2002)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: W491@. (Aberle et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective (with wit^A12) (Ball et al., 2010) neurophysiology defective \| third instar larval stage (with wit^A12) (Ball et al., 2010) size defective \| third instar larval stage (with wit^A12) (Ball et al., 2010)
Phenotype Manifest In
	embryonic/larval neuromuscular junction \| third instar larval stage (with wit^A12) (Ball et al., 2010) NMJ bouton \| third instar larval stage (with wit^A12) (Ball et al., 2010)
Detailed Description
	Statement Reference wit[A12]/wit[HA3] third instar larvae exhibit neuromuscular junction overgrowth and a reduced number of boutons per muscle surface area in muscle 4 compared to controls. (Ball et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference wit^B11/wit^HA3 has neuroanatomy defective phenotype, suppressible by tkv::wit^{tkv-EC.wit-IC.Ubi-p63E}/tkv::wit^{wit-EC.tkv-IC.Ubi-p63E} (McCabe et al., 2004) wit^HA3/wit^A12 has neuroanatomy defective \| third instar larval stage phenotype, suppressible \| partially by Scer\GAL4^BG380/trio^Scer\UAS.cBa (Ball et al., 2010)
NOT suppressed by
Statement Reference wit^HA3/wit^A12 has neuroanatomy defective \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^{Scer\UAS.T:Hsap\MYC} (Ball et al., 2010) wit^HA3/wit^A12 has neuroanatomy defective \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^V12.Scer\UAS (Ball et al., 2010) wit^HA3/wit^A12 has neurophysiology defective \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^Scer\UAS.cLa (Ball et al., 2010) wit^HA3/wit^A12 has size defective \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^{Scer\UAS.T:Hsap\MYC} (Ball et al., 2010) wit^HA3/wit^A12 has size defective \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^V12.Scer\UAS (Ball et al., 2010)
Suppressor of
Statement Reference wit^HA3/wit^A12 is a suppressor of neuroanatomy defective \| third instar larval stage phenotype of Rac1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^BG380 (Ball et al., 2010) wit^HA3/wit^A12 is a suppressor of neurophysiology defective \| third instar larval stage phenotype of Rac1^Scer\UAS.cLa, Scer\GAL4^BG380 (Ball et al., 2010) wit^HA3/wit^A12 is a suppressor of size defective \| third instar larval stage phenotype of Rac1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^BG380 (Ball et al., 2010)
NOT Suppressor of
Statement Reference wit^HA3/wit^A12 is a non-suppressor of neuroanatomy defective \| third instar larval stage phenotype of Rac1^V12.Scer\UAS, Scer\GAL4^BG380 (Ball et al., 2010) wit^HA3/wit^A12 is a non-suppressor of size defective \| third instar larval stage phenotype of Rac1^V12.Scer\UAS, Scer\GAL4^BG380 (Ball et al., 2010)
Other
Statement Reference Mad[+]/Mad¹, wit^HA3 has neuroanatomy defective phenotype (McCabe et al., 2004) Mad¹, wit^HA3/wit[+] has neuroanatomy defective \| dominant phenotype (McCabe et al., 2004) Med[+]/Med^C246, wit^HA3 has neuroanatomy defective phenotype (McCabe et al., 2004) Med^C246, wit^HA3/wit[+] has neuroanatomy defective \| dominant phenotype (McCabe et al., 2004) sax⁴, wit^HA3/wit[+] has neuroanatomy defective \| dominant phenotype (McCabe et al., 2004) sax⁴/sax[+], wit^HA3 has neuroanatomy defective phenotype (McCabe et al., 2004) tkv⁸, wit^HA3/wit[+] has neuroanatomy defective \| dominant phenotype (McCabe et al., 2004) tkv⁸/tkv[+], wit^HA3 has neuroanatomy defective phenotype (McCabe et al., 2004)
Phenotype Manifest In
Suppressed by
Statement Reference wit^B11/wit^HA3 has neuromuscular junction phenotype, suppressible by tkv::wit^{tkv-EC.wit-IC.Ubi-p63E}/tkv::wit^{wit-EC.tkv-IC.Ubi-p63E} (McCabe et al., 2004) wit^HA3/wit^A12 has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, suppressible \| partially by Scer\GAL4^BG380/trio^Scer\UAS.cBa (Ball et al., 2010) wit^HA3/wit^A12 has NMJ bouton \| third instar larval stage phenotype, suppressible \| partially by Scer\GAL4^BG380/trio^Scer\UAS.cBa (Ball et al., 2010)
NOT suppressed by
Statement Reference wit^HA3/wit^A12 has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^{Scer\UAS.T:Hsap\MYC} (Ball et al., 2010) wit^HA3/wit^A12 has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^V12.Scer\UAS (Ball et al., 2010) wit^HA3/wit^A12 has NMJ bouton \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^{Scer\UAS.T:Hsap\MYC} (Ball et al., 2010) wit^HA3/wit^A12 has NMJ bouton \| third instar larval stage phenotype, non-suppressible by Scer\GAL4^BG380/Rac1^V12.Scer\UAS (Ball et al., 2010)
Suppressor of
Statement Reference wit^HA3/wit^A12 is a suppressor of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of Rac1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^BG380 (Ball et al., 2010) wit^HA3/wit^A12 is a suppressor of NMJ bouton \| third instar larval stage phenotype of Rac1^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^BG380 (Ball et al., 2010)
NOT Suppressor of
Statement Reference wit^HA3/wit^A12 is a non-suppressor of embryonic/larval neuromuscular junction \| third instar larval stage phenotype of Rac1^V12.Scer\UAS, Scer\GAL4^BG380 (Ball et al., 2010) wit^HA3/wit^A12 is a non-suppressor of NMJ bouton \| third instar larval stage phenotype of Rac1^V12.Scer\UAS, Scer\GAL4^BG380 (Ball et al., 2010)
Other
Statement Reference Mad¹, wit^HA3/wit[+] has bouton phenotype (McCabe et al., 2004) Mad¹, wit^HA3/wit[+] has neuromuscular junction phenotype (McCabe et al., 2004) Mad¹, wit^HA3 has bouton phenotype (McCabe et al., 2004) Mad¹, wit^HA3 has neuromuscular junction phenotype (McCabe et al., 2004) Med^C246, wit^HA3/wit[+] has bouton phenotype (McCabe et al., 2004) Med^C246, wit^HA3/wit[+] has neuromuscular junction phenotype (McCabe et al., 2004) Med^C246, wit^HA3 has bouton phenotype (McCabe et al., 2004) Med^C246, wit^HA3 has neuromuscular junction phenotype (McCabe et al., 2004) sax⁴, wit^HA3/wit[+] has bouton phenotype (McCabe et al., 2004) sax⁴, wit^HA3/wit[+] has neuromuscular junction phenotype (McCabe et al., 2004) sax⁴, wit^HA3 has bouton phenotype (McCabe et al., 2004) sax⁴, wit^HA3 has neuromuscular junction phenotype (McCabe et al., 2004) tkv⁸, wit^HA3/wit[+] has bouton phenotype (McCabe et al., 2004) tkv⁸, wit^HA3/wit[+] has neuromuscular junction phenotype (McCabe et al., 2004) tkv⁸, wit^HA3 has bouton phenotype (McCabe et al., 2004) tkv⁸, wit^HA3 has neuromuscular junction phenotype (McCabe et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Expression of wit[A12]/wit[HA3] suppresses the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1[Scer\UAS.T:Hsap\MYC] is expressed in motor neurons under the control of Scer\GAL4[BG380], instead producing a reduction in bouton number similar to the phenotype seen in wit[A12]/wit[HA3] third instar larvae. Expression of wit[A12]/wit[HA3] fails to suppress the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1[V12.Scer\UAS] is expressed in motor neurons under the control of Scer\GAL4[BG380]. wit[A12]/wit[HA3] suppresses the increase in evoked excitatory junctional potential (EJPs) and quantal content seen when Rac1[Scer\UAS.cLa] is expressed in motor neurons under the control of Scer\GAL4[BG380], with third instar larvae instead displaying the reduction in neurotransmitter release phenotype seen in wit[A12]/wit[HA3] mutants. Expression of trio[Scer\UAS.cBa] in motor neurons under the control of Scer\GAL4[BG380] partially suppresses the reduced bouton number phenotype seen in wit[A12]/wit[HA3] larval neuromuscular junctions. (Ball et al., 2010) The number of synaptic boutons/muscle surface area at muscle 6/7 in Med^C246/wit^HA3 double heterozygotes is 91.3 +/- 2.7 % of wild type. The number of synaptic boutons/muscle surface area at muscle 6/7 in Mad¹/wit^HA3 double heterozygotes is 95.9 +/- 2.2 % of wild type. The number of synaptic boutons/muscle surface area at muscle 6/7 in sax⁴/wit^HA3 double heterozygotes is 85.2 +/- 3.6 % of wild type. The number of synaptic boutons/muscle surface area at muscle 6/7 in tkv⁸/wit^HA3 double heterozygotes is 88.8 +/- 2.4 % of wild type. Co-expression of tkv::wit^{wit-EC.tkv-IC.Ubi-p63E} and tkv::wit^{tkv-EC.wit-IC.Ubi-p63E} rescues the neuromuscular junction size in wit^HA3/wit^B11 animals. (McCabe et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	wit^HA3 (Collins et al., 2006, Xiong et al., 2010, Ball et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Ball et al., 2010, Neuron 66(4): 536--549 Retrograde BMP Signaling Controls Synaptic Growth at the NMJ by Regulating Trio Expression in Motor Neurons. [FBrf0210920] Xiong et al., 2010, J. Cell Biol. 191(1): 211--223 Protein turnover of the Wallenda/DLK kinase regulates a retrograde response to axonal injury. [FBrf0211939] Collins et al., 2006, Neuron 51(1): 57--69 Highwire restrains synaptic growth by attenuating a MAP kinase signal. [FBrf0194919] McCabe et al., 2004, Neuron 41(6): 891--905 Highwire regulates presynaptic BMP signaling essential for synaptic growth. [FBrf0175022] Aberle et al., 2002, Neuron 33(4): 545--558 wishful thinking Encodes a BMP Type II Receptor that Regulates Synaptic Growth in Drosophila. [FBrf0145177]

Allele Dmel\witHA3

Allele Dmel\wit^HA3