Allele Dmel\wit^C175

General Information
Symbol	Dmel\wit^C175	Species	D. melanogaster
Name		FlyBase ID	FBal0133970
Feature type	allele	Associated gene	Dmel\wit

Allele class
Mutagen
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The Df(3L)C175 breakpoint is in the 3' portion of the wit transcription unit. (Marques et al., 2002)
Caused by aberration	Df(3L)C175 (Marques et al., 2002, Aberle et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pharate adult stage (with wit^A12) (Marques et al., 2002) neurophysiology defective (with wit^A12) (Marques et al., 2002)
Phenotype Manifest In
	femur \| pharate adult stage (with wit^A12) (Marques et al., 2002) leg \| pharate adult stage (with wit^A12) (Marques et al., 2002) metathoracic femur \| pharate adult stage (with wit^A12) (Marques et al., 2002) metathoracic tibia \| pharate adult stage (with wit^A12) (Marques et al., 2002) postsynaptic membrane (with wit^A12) (Marques et al., 2002) tarsal segment \| pharate adult stage (with wit^A12) (Marques et al., 2002) tibia \| pharate adult stage (with wit^A12) (Marques et al., 2002)
Detailed Description
	Statement Reference wit^A12/wit^C175 transheterozygous mutants are completely lethal. Approximately 50% of animals survive to the pharate stage. The remaining animals die during larval and early pupal stages. Those animals that do survive to pharate adult stage exhibit the head expansion and abdominal peristaltic movements characteristic of normal pre-eclosion behaviour, but do not rupture nor escape from the pupal case. Many pharate adults show a bend in the femur or tibia, the phenotype being more pronounced in the metathoracic leg. A small fraction of the animals also exhibit truncations of the tarsal segments. The general organisation of the nervous system in these mutants is essentially normal. wit^A12/wit^C175 mutant larvae exhibit dramatically reduced transmitter release from presynaptic terminals when the excitatory junctional current (EJC) of body wall muscles examined. The amplitude of EJCs at 0.8 mM Ca²⁺ is significantly reduced in mutants. Most nerve stimuli fail to evoke transmitter release at low extracellular ^Ca<up>2+</up> in mutants. Cooperativity is unaffected in mutants. The amplitude of miniature excitatory junction potentials (mEJPs) is not significantly altered in mutants. (Marques et al., 2002)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	wit^A12/wit^C175 is partially rescued by Scer\GAL4^elav-C155/wit^Scer\UAS.cMa (Marques et al., 2002)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	wit^C175
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Marques et al., 2003, Development 130(22): 5457--5470 Retrograde Gbb signaling through the Bmp type 2 receptor wishful thinking regulates systemic FMRFa expression in Drosophila. [FBrf0167500] Aberle et al., 2002, Neuron 33(4): 545--558 wishful thinking Encodes a BMP Type II Receptor that Regulates Synaptic Growth in Drosophila. [FBrf0145177] Marques et al., 2002, Neuron 33(4): 529--543 The Drosophila BMP Type II receptor wishful thinking regulates neuromuscular synapse morphology and function. [FBrf0145176]

Allele Dmel\witC175

Allele Dmel\wit^C175