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General Information
Symbol
Dmel\slpr921
Species
D. melanogaster
Name
FlyBase ID
FBal0134000
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:

    G8175198A

    Reported nucleotide change:

    G?A

    Amino acid change:

    E324K | slpr-PA; E324K | slpr-PB; E324K | slpr-PC; E324K | slpr-PD

    Reported amino acid change:

    E324K

    Reported amino acid change:

    E?K

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Nucleotide substitution: G?A.

    Amino acid replacement: E324K.

    Amino acid replacement: E324K. Nucleotide substitution: G?A.

    Amino acid replacement: E?K. Mutation is within the kinase domain.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    100% of mutant embryos have a severe dorsal open phenotype.

    slpr921 displays complete embryonic lethality - dead embryos exhibit a high proportion of severe 'dorsal open' (a hole in the dorsal larval cuticle) phenotypes.

    slpr921/Y mutants (lacking zygotic slpr function) are embryonic lethal with a severe dorsal closure phenotype. Mutant embryos derived from homozygous female germline clones (lacking both zygotic and maternal slpr function) show the same cuticle phenotype as zygotically mutant embryos.

    Eggs laid by females carrying homozygous follicle cell clones have thin, shortened dorsal appendages.

    20% of slpr921/slprBS06 escaper adult females have maxillary palp defects.

    slpr921/Y embryos derived from heterozygous females die during embryogenesis with a severe dorsal open phenotype. The concerted elongation of leading edge cells that is seen during dorsal closure initiates correctly in the embryos, but fails shortly afterwards and eventually the cells round up, the dorsal epidermis slackens laterally and the internal organs herniate. A paternal copy of slpr+ can rescue embryos derived from females containing slpr921 homozygous germ-line clones. Mutant embryos derived from females containing homozygous germ-line clones which do not receive a paternal copy of slpr+ die with a severe cuticle phenotype identical to the that of slpr921 zygotic mutants.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressed by
    Suppressor of
    Statement
    Reference

    slpr921/slpr[+] is a suppressor of visible phenotype of Rac1GMR.PN

    NOT Suppressor of
    Statement
    Reference

    slpr921/slpr[+] is a non-suppressor of visible phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, egrGS9830

    Phenotype Manifest In
    Suppressed by
    NOT suppressed by
    Enhancer of
    Statement
    Reference
    NOT Enhancer of
    Statement
    Reference

    slpr921/slpr[+] is a non-enhancer of eye phenotype of hepCA.sev.Tag:MYC

    Suppressor of
    Statement
    Reference

    slpr921/slpr[+] is a suppressor of eye phenotype of Rac1GMR.PN

    slpr921/slpr[+] is a suppressor of ommatidium phenotype of Rac1GMR.PN

    NOT Suppressor of
    Statement
    Reference

    slpr921/slpr[+] is a non-suppressor of eye phenotype of hepCA.sev.Tag:MYC

    slpr921/slpr[+] is a non-suppressor of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, egrGS9830

    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Expression of slpr::Tak1STCt.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS partially rescues the dorsal closure phenotype seen in slpr921 mutant males. Some embryos exhibit head defects.

    Expression of slpr::Tak1STK.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS partially rescues the dorsal closure phenotype seen in slpr921 mutant males. Some embryos exhibit head defects.

    Expression of slpr::Tak1SAAATCt.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS does not rescue the dorsal closure phenotype seen in slpr921 mutant males. Some embryos exhibit head defects.

    Expression of slpr::Tak1TSK.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS does not rescue the dorsal closure phenotype seen in slpr921 mutant males. Some embryos exhibit head defects.

    Expression of slpr::Tak1TSAAA.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS does not rescue the dorsal closure phenotype seen in slpr921 mutant males. Some embryos exhibit head defects.

    Expression of Tak1Ct.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS does not rescue the dorsal closure phenotype seen in slpr921 mutant males. Some embryos exhibit head defects.

    alphXS-88/alphXS-88 or alphS-331/alphS-331 significantly reduces the number of dead embryos exhibiting a severe 'dorsal open' phenotype, while increasing the proportion the either display a weak 'dorsal open' phenotype or have only head defects.

    The eye ablation phenotype seen in adults expressing egrGS9830 under the control of Scer\GAL4GMR.PF is not suppressed by slpr921/+.

    The severe dorsal open phenotype of slpr921/Y embryos is significantly rescued by expression of JraAsp.hs.sev (using a 30 minute heat shock several hours before the beginning of dorsal closure) and is also significantly rescued by pucE69/+.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments

    Expression of slprScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS rescues the dorsal closure phenotype seen in slpr921 mutant males. Some embryos exhibit head defects.

    Expression of slprSKLC.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS partially rescues the dorsal closure phenotype seen in slpr921 mutant males. Some embryos exhibit head defects.

    Expression of slprAAA.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS does not rescue the dorsal closure phenotype seen in slpr921 mutant males. Some embryos exhibit head defects.

    The lethality of slpr921 mutants is completely rescued by expression of slprScer\UAS.P\T.cPa under the control of Scer\GAL4arm.PS.

    The lethality of slpr921 mutants is partially rescued by expression of slprScer\UAS.P\T.T:Ivir\HA1 or slprSKLC.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS.

    The lethality of slpr921 mutants is not rescued by expression of slprAAA.Scer\UAS.P\T.T:Ivir\HA1, slprTAA.Scer\UAS.P\T.T:Ivir\HA1, slprASA.Scer\UAS.P\T.T:Ivir\HA1, slprSKLC.AAA.Scer\UAS.P\T.T:Ivir\HA1, slprSH3.Scer\UAS.P\T.T:Ivir\HA1, slprAVA.Scer\UAS.P\T.T:Ivir\HA1, slprCterm.Scer\UAS.P\T.T:Ivir\HA1 or slprΔSH3.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS.

    Expression of slprAAT.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS in slpr921 mutants results in the recovery of a few rescued adults and many more rescued animals are observed as pharate adults.

    Expression of slprSH3.Scer\UAS.P\T.T:Ivir\HA1, slprSKLC.AAA.Scer\UAS.P\T.T:Ivir\HA1 or slprCterm.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS fails to rescue the severe dorsal open phenotype of slpr921 embryos.

    Expression of slprSKLC.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS significantly rescues the severe dorsal open phenotype of slpr921 embryos (the penetrance of the phenotype is reduced from 100% to approximately 30%).

    Expression of slprΔSH3.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS significantly rescues the severe dorsal open phenotype of slpr921 embryos (the penetrance of the phenotype is reduced from 100% to approximately 20%).

    Expression of slprScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4arm.PS significantly rescues the severe dorsal open phenotype of slpr921 embryos (the penetrance of the phenotype is reduced from 100% to approximately 10%).

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    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (8)