Allele Dmel\E2f2³²⁹

General Information
Symbol	Dmel\E2f2³²⁹	Species	D. melanogaster
Name		FlyBase ID	FBal0134271
Feature type	allele	Associated gene	Dmel\E2f2

Map ( GBrowse )
Allele class
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	Delta2-3 (Cayirlioglu et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References deletion 2L:21,154,665..21,156,899 evidence=experimental comment=Excision deletion extending from the P{lacW}Mpp6^k16402a insertion site to 11bp after the E2f2 stop codon. (Cayirlioglu et al., 2001)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{lacW}Mpp6^k16402a (Cayirlioglu et al., 2001)
Nature of the lesion	Statement Reference Imprecise excision of the P{lacW} element resulting in a deletion with one breakpoint at the site of the P{lacW} insertion and the other 11bp downstream of the E2f2 stop codon, near the end of the E2f2 transcription unit. (Cayirlioglu et al., 2001)
Caused by aberration	Df(2L)E2f2³²⁹ (Cayirlioglu et al., 2001, Wen et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile \| partially, with Df(2L)DS8, E2f2^1-188 (Cayirlioglu et al., 2001) female sterile \| partially (with Df(2L)DS8), with E2f2^1-188 (Cayirlioglu et al., 2001) female sterile \| partially \| recessive (Cayirlioglu et al., 2001) male fertile (Cayirlioglu et al., 2001) viable (Cayirlioglu et al., 2001) viable, with E2f2^1-188 (Cayirlioglu et al., 2003)
Phenotype Manifest In
	chorion (Cayirlioglu et al., 2001) chorion, with Df(2L)DS8, E2f2^1-188 (Cayirlioglu et al., 2001) chorion (with Df(2L)DS8), with E2f2^1-188 (Cayirlioglu et al., 2001) follicle cell, with E2f2^1-188 (Cayirlioglu et al., 2003) follicle cell & nucleus (Cayirlioglu et al., 2001) follicle cell & nucleus, with Df(2L)DS8, E2f2^1-188 (Cayirlioglu et al., 2001) follicle cell & nucleus (with Df(2L)DS8), with E2f2^1-188 (Cayirlioglu et al., 2001)
Detailed Description
	Statement Reference E2f2^76Q1/E2f2³²⁹ Mpp6^+t4 animals have a pattern of irradiation induced apoptosis in the late third instar wing disc which is identical to that seen in wild-type animals. (Mpp6^+t4 is present to rescued loss of Mpp6 in Df(2L)E2f2³²⁹. (Moon et al., 2005) Nearly all follicle cells in stage 13 E2f2[329]/E2f2[1-188] egg chambers show a phenotype of ectopic genomic replication, as assayed by BrdU incorporation. This phenotype is fully penetrant. (Cayirlioglu et al., 2003) 10% of eggs laid by E2f2³²⁹ females (Df(2L)E2f2³²⁹ homozygotes with Mpp6 function rescued by Mpp6^+t4) hatch. 97% of the eggs laid by E2f2³²⁹ females have chorion defects. The chorions are more translucent than normal, indicating that the eggshell is thin. In stage 10B through 13 egg chambers of E2f2³²⁹ females (Df(2L)E2f2³²⁹ homozygotes with Mpp6 function rescued by Mpp6^+t4), BrdU incorporation is seen throughout the entire follicle cell nuclei, rather than in characteristic subnuclear foci (which is seen in wild type). The follicle cells do not undergo an additional endocycle in these females. The number of BrdU positive follicle cell nuclei in pre-stage 10B egg chambers of these females is similar to wild type. E2f2³²⁹ males (Df(2L)E2f2³²⁹ homozygotes with Mpp6 function rescued by Mpp6^+t4) are fully fertile. 8% of eggs laid by E2f2³²⁹/Df(2L)DS8 females carrying E2f2^1-188 (Df(2L)E2f2³²⁹/Df(2L)DS8 females carrying E2f2^1-188 with Mpp6 function rescued by Mpp6^+t1-188) hatch. 93% of the eggs laid by these females have chorion defects. In stage 10B through 13 egg chambers of E2f2³²⁹/Df(2L)DS8 females carrying E2f2^1-188 (Df(2L)E2f2³²⁹/Df(2L)DS8 females carrying E2f2^1-188 with Mpp6 function rescued by Mpp6^+t1-188), BrdU incorporation is seen throughout the entire follicle cell nuclei, rather than in characteristic subnuclear foci (which is seen in wild type). The follicle cells do not undergo an additional endocycle in these females. (Cayirlioglu et al., 2001)
External Data
Linkouts
Interactions
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Phenotypic Class
Other
Statement Reference E2f2³²⁹/E2f2^1-188, E2fⁱ²/E2f⁹¹ has lethal phenotype (Cayirlioglu et al., 2003)
Phenotype Manifest In
Suppressed by
Statement Reference E2f2^1-188, E2f2³²⁹ has follicle cell phenotype, suppressible \| partially by E2f[+]/E2f⁹¹ (Cayirlioglu et al., 2003) E2f2^1-188, E2f2³²⁹ has follicle cell phenotype, suppressible \| partially by Mcm2^rL074/Mcm2[+] (Cayirlioglu et al., 2003) E2f2^1-188, E2f2³²⁹ has follicle cell phenotype, suppressible \| partially by Orc2[+]/Orc2^γ3 (Cayirlioglu et al., 2003) E2f2^1-188, E2f2³²⁹ has follicle cell phenotype, suppressible \| partially by Orc5¹/Orc5[+] (Cayirlioglu et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference The ectopic genomic replication that is seen in the follicle cells of stage 13 E2f2[329]/E2f2[1-188] egg chambers is partially suppressed by Orc2[γ3]/+ or Mcm2[rL074]/+; there is an approximate 65% reduction in the number of follicle cells within an egg chamber that display ectopic genomic replication and there is an apparent reduction in the intensity of ectopic BrdU incorporation. The ectopic genomic replication that is seen in the follicle cells of stage 13 E2f2[329]/E2f2[1-188] egg chambers is partially suppressed by Orc5[1]; the phenotype is suppressed in 36% of egg chambers. The ectopic genomic replication that is seen in the follicle cells of stage 13 E2f2[329]/E2f2[1-188] egg chambers is partially suppressed by E2f[91]/+; 55% of the double mutant egg chambers show a 50% reduction in the number of follicle cells exhibiting ectopic genomic replication compared to the single mutant egg chambers. (Cayirlioglu et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	E2f2³²⁹ is rescued by E2f2^+t6.4 (Cayirlioglu et al., 2001)
Comments	E2f2^+t6.4 rescues the chorion defects of eggs derived from E2f2³²⁹ females (Df(2L)E2f2³²⁹ homozygotes with Mpp6 function rescued by Mpp6^+t4). The follicle cell BrdU incorporation of these females is also rescued by E2f2^+t6.4. (Cayirlioglu et al., 2001)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	de2f2³²⁹ (Moon et al., 2005) E2f2³²⁹ (Cayirlioglu et al., 2003, Wen et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Wen et al., 2008, Genes Dev. 22(5): 601--614 Epigenetic regulation of gene expression by Drosophila Myb and E2F2-RBF via the Myb-MuvB/dREAM complex. [FBrf0202994] Moon et al., 2005, Dev. Cell 9(4): 463--475 Drosophila E2F1 has context-specific pro- and antiapoptotic properties during development. [FBrf0190216] Cayirlioglu et al., 2003, Mol. Cell. Biol. 23(6): 2123--2134 Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells. [FBrf0159210] Cayirlioglu et al., 2001, Development 128(24): 5085--5098 Drosophila E2f2 promotes the conversion from genomic DNA replication to gene amplification in ovarian follicle cells. [FBrf0141502]

Allele Dmel\E2f2329

Allele Dmel\E2f2³²⁹