CblKG03080 homozygous mutant third instar larvae have significantly increased concentration of circulating hemocytes compared to wild-type controls due to an increase in their mitotic activity (higher percentage of pH3-positive hemocytes). Primary hemocytes from these mutant larvae show strongly reduced internalization of sSpi-GFP protein (spi encodes the principal ligand of the EGFR pathway) at high sSpi-GFP conditions (treatment with 30 ng/ml sSpi-GFP), as compared to controls.
Homozygous CblKG03080 flies show poor survival to adulthood.
Less than half of CblEY11427/CblKG03080 mutant males survive to adulthood.
Pupation is delayed by approximately 24 hours in CblEY11427/CblKG03080 larvae compared with wild-type controls.
CblEY11427/CblKG03080 males show increased sensitivity to starvation, exhibiting an approximately 20% decrease in the median survival time upon food deprivation.
CblEY11427/CblKG03080 males display a dramatically shortened lifespan, with an approximately 67% reduction in the median lifespan compared with wild-type controls.
Heterozygotes show a direct effect on the shape of the wing.