FB2025_01 , released February 20, 2025
Allele: Dmel\wRNAi.Sym.UAS
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General Information
Symbol
Dmel\wRNAi.Sym.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0135571
Feature type
allele
Associated gene
Dmel\w
Associated Insertion(s)
Carried in Construct
P{Sym-UAS-w}
Key Links
Transgenic product class
RNAi_reagent
(Giordano et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Giordano et al., 2002)
Mutagen
in vitro construct
(Giordano et al., 2002)
Progenitor genotype
Carried in construct
P{Sym-UAS-w}
Cytology
Description

Sense-antisense transcription (driven by two convergent arrays of UAS regulatory sequences) of a 1.4kb genomic fragment spanning exons 2-4 of w results in double stranded RNA (dsRNA) expression.

(Giordano et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
w
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Eye colour: expression of wdsRNA.Sym.Scer\UAS (carried in P{Sym-UAS-w}) under the control of Scer\GAL4Act5C.PI (carried in P{Act5C-GAL4}) strongly silences expression of w+mC (carried in both P{Sym-UAS-w} and P{Act5C-GAL4}); flies carrying both P{Sym-UAS-w} and P{Act5C-GAL4} have lighter eye colour than those carrying either P{Sym-UAS-w} or P{Act5C-GAL4} alone in a w1118 background. Silencing is not seen in the absence of Scer\GAL4 expression; flies carrying both P{Sym-UAS-w} and P{AyGAL4} (a transgene in which Scer\GAL4 is not expressed due to Scer\FRT sites between the Act5C promoter and Scer\GAL4 coding region) do not show silencing of w+mC expression.

      Eye colour: expression of wdsRNA.Sym.Scer\UAS (carried in P{Sym-UAS-w}) under the control of Scer\GAL4Act5C.PI in w[+]/w[-] heterozygous females results in an appreciable reduction in eye pigmentation. The red pigment content is about 10% that of control flies.

      Eye colour: expression of wdsRNA.Sym.Scer\UAS (carried in P{Sym-UAS-w}) under the control of Scer\GAL4αTub84B.PL strongly silences expression of w in w[+]/w[-] heterozygous females; there is a dramatic reduction in eye pigment level.

      Eye colour: expression of wdsRNA.Sym.Scer\UAS (carried in P{Sym-UAS-w}) under the control of Scer\GAL4elav-C155 appreciably silences expression of w in w[+]/w[-] heterozygous females.

      Eye colour: expression of wdsRNA.Sym.Scer\UAS (carried in P{Sym-UAS-w}) under the control of Scer\GAL4hs.2sev results in female flies with red/brown mottled eyes in a w[+]/w[-] background.

      (Giordano et al., 2002)

      Eye colour: simultaneous expression of wSym.Scer\UAS and wa.Sym.Scer\UAS (both carried in P{Sym-UAS-w}) under the control of Scer\GAL4Act5C.PI (carried in P{Act5C-GAL4}) strongly silences expression of w+mC (carried in both P{Sym-UAS-w} and P{Act5C-GAL4}); flies carrying both P{Sym-UAS-w} and P{Act5C-GAL4} have lighter eye colour than those carrying either P{Sym-UAS-w} or P{Act5C-GAL4} alone in a w1118 background. Silencing is not seen in the absence of Scer\GAL4 expression; flies carrying both P{Sym-UAS-w} and P{AyGAL4} (a transgene in which Scer\GAL4 is not expressed due to Scer\FRT sites between the Act5C promoter and Scer\GAL4 coding region) do not show silencing of w+mC expression. Eye colour: simultaneous expression of wSym.Scer\UAS and wa.Sym.Scer\UAS (both carried in P{Sym-UAS-w}) under the control of Scer\GAL4Act5C.PI in w+/w- heterozygous females results in an appreciable reduction in eye pigmentation. The red pigment content is about 10% that of control flies. Eye colour: simultaneous expression of wSym.Scer\UAS and wa.Sym.Scer\UAS (both carried in P{Sym-UAS-w}) under the control of Scer\GAL4αTub84B.PL strongly silences expression of w in w+/w- heterozygous females; there is a dramatic reduction in eye pigment level. Eye colour: simultaneous expression of wSym.Scer\UAS and wa.Sym.Scer\UAS (both carried in P{Sym-UAS-w}) under the control of Scer\GAL4elav-C155 appreciably silences expression of w in w+/w- heterozygous females. Eye colour: simultaneous expression of wSym.Scer\UAS and wa.Sym.Scer\UAS (both carried in P{Sym-UAS-w}) under the control of Scer\GAL4hs.2sev results in female flies with red/brown mottled eyes in a w+/w- background.

      (Giordano et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      wRNAi.Sym.UAS
      wSym.Scer\UAS
      wdsRNA.Sym.Scer\UAS
      wdsRNA.Sym.UAS
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0135569
      References (2)