Allele Dmel\w^{dsRNA.Sym.Scer\UAS}

General Information
Symbol	Dmel\w^{dsRNA.Sym.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0135571
Feature type	allele	Associated gene	Dmel\w

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Giordano et al., 2002) in vitro construct - RNAi (Giordano et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Sense-antisense transcription (driven by two convergent arrays of Scer\UAS promoter and regulatory sequences) of a 1.4 kb genomic fragment spanning exons 2-4 of w results in double stranded RNA (dsRNA) expression. (Giordano et al., 2002)
Carried in construct	P{Sym-UAS-w} (Giordano et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	eye color defective, with Scer\GAL4^Act5C.PI (Giordano et al., 2002) eye color defective, with Scer\GAL4^Act5C.PI, w^+mC (Giordano et al., 2002) eye color defective, with Scer\GAL4^elav-C155 (Giordano et al., 2002) eye color defective, with Scer\GAL4^hs.2sev (Giordano et al., 2002) eye color defective, with Scer\GAL4^αTub84B.PL (Giordano et al., 2002)
Phenotype Manifest In
	pigment cell, with Scer\GAL4^Act5C.PI (Giordano et al., 2002) pigment cell, with Scer\GAL4^Act5C.PI, w^+mC (Giordano et al., 2002) pigment cell, with Scer\GAL4^elav-C155 (Giordano et al., 2002) pigment cell, with Scer\GAL4^hs.2sev (Giordano et al., 2002) pigment cell, with Scer\GAL4^αTub84B.PL (Giordano et al., 2002)
Detailed Description
	Statement Reference Eye colour: expression of w[dsRNA.Sym.Scer\UAS] (carried in P{Sym-UAS-w}) under the control of Scer\GAL4[Act5C.PI] (carried in P{Act5C-GAL4}) strongly silences expression of w[+mC] (carried in both P{Sym-UAS-w} and P{Act5C-GAL4}); flies carrying both P{Sym-UAS-w} and P{Act5C-GAL4} have lighter eye colour than those carrying either P{Sym-UAS-w} or P{Act5C-GAL4} alone in a w[1118] background. Silencing is not seen in the absence of Scer\GAL4 expression; flies carrying both P{Sym-UAS-w} and P{AyGAL4} (a transgene in which Scer\GAL4 is not expressed due to Scer\FRT sites between the Act5C promoter and Scer\GAL4 coding region) do not show silencing of w[+mC] expression. Eye colour: expression of w[dsRNA.Sym.Scer\UAS] (carried in P{Sym-UAS-w}) under the control of Scer\GAL4[Act5C.PI] in w[+]/w[-] heterozygous females results in an appreciable reduction in eye pigmentation. The red pigment content is about 10% that of control flies. Eye colour: expression of w[dsRNA.Sym.Scer\UAS] (carried in P{Sym-UAS-w}) under the control of Scer\GAL4[αTub84B.PL] strongly silences expression of w in w[+]/w[-] heterozygous females; there is a dramatic reduction in eye pigment level. Eye colour: expression of w[dsRNA.Sym.Scer\UAS] (carried in P{Sym-UAS-w}) under the control of Scer\GAL4[elav-C155] appreciably silences expression of w in w[+]/w[-] heterozygous females. Eye colour: expression of w[dsRNA.Sym.Scer\UAS] (carried in P{Sym-UAS-w}) under the control of Scer\GAL4[hs.2sev] results in female flies with red/brown mottled eyes in a w[+]/w[-] background. (Giordano et al., 2002)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	w^{dsRNA.Sym.Scer\UAS} w^Sym.Scer\UAS
Name Synonym
Secondary FlyBase IDs
	FBal0135569
References ( 1 )
Research paper	Giordano et al., 2002, Genetics 160(2): 637--648 RNAi triggered by symmetrically transcribed transgenes in Drosophila melanogaster. [FBrf0144904]

Allele Dmel\wdsRNA.Sym.Scer\UAS

Allele Dmel\w^{dsRNA.Sym.Scer\UAS}