Open Close
General Information
Symbol
Dmel\sub1794
Species
D. melanogaster
Name
FlyBase ID
FBal0135614
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G17747524A

Amino acid change:

C152Y | sub-PA; C152Y | sub-PB

Reported amino acid change:

C152Y

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: C152Y.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of subCT.Scer\UAS.P\T.T:Avic-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in sub1794/+ females results in reduced numbers of progeny.

Expression of subCT.Scer\UAS.P\T.T:Avic-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in sub1794/sub1 females results in sterility.

Elevated frequency of X, fourth and second chromosome non-disjunction. Autosomal chromosome loss is significant. Most or all of the non-disjunction involves homologs at meiosis I. The position of the chiasma seems to have little effect on whether the homologs will fail to segregate normally. Achiasmate chromosomes require sub for segregation, whereas chiasmate bivalents can in some instances segregate correctly. Transheterozygotes with sub1 show elevated levels of X chromosome non-disjunction in females. Stage 14 oocytes of sub1794 homozygotes and transheterozygotes with subDub show a defect in spindle pole formation - most often spindles are either monopolar or tripolar, karyosome may be split, spindles broken or misshapen such that they fray, or do not taper at the poles. When heterozygous with Df(2R)PclXM82 a higher frequency of spindle defects results, though the severity of spindle defects when they occur is unchanged. In stage 14 oocytes of sub1794/Df(2R)PC4 females the centromeres are often abnormally arranged: either on the same side of the karyosome or, if the karyosome is split, in the same mass of chromosomes. Eggs derived from sub1794 mothers show some abnormalities in spindle morphology during early mitoses, though later stage embryos develop normally suggesting recovery from the early defect mitotic defect.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

sub1794 has meiotic cell cycle defective phenotype, enhanceable by ncdD/ncd[+]

sub1794 has meiotic cell cycle defective phenotype, enhanceable by nod[+]/nod4

NOT Enhanced by
Statement
Reference

sub1794 has meiotic cell cycle defective phenotype, non-enhanceable by ncd[+]/ncd1

Enhancer of
Statement
Reference

sub[+]/sub1794 is an enhancer of meiotic cell cycle defective phenotype of ncdD

sub[+]/sub1794 is an enhancer of meiotic cell cycle defective phenotype of nod4

NOT Enhancer of
Statement
Reference

sub[+]/sub1794 is a non-enhancer of meiotic cell cycle defective phenotype of ncd1

Phenotype Manifest In
Enhanced by
Statement
Reference

sub1794 has karyosome phenotype, enhanceable by ncdD/ncd[+]

sub1794 has spindle phenotype, enhanceable by ncdD/ncd[+]

NOT Enhanced by
Statement
Reference

sub1794 has spindle phenotype, non-enhanceable by ncd1/ncd1

Enhancer of
Statement
Reference

sub[+]/sub1794 is an enhancer of karyosome phenotype of ncdD

sub[+]/sub1794 is an enhancer of spindle phenotype of ncdD

Additional Comments
Genetic Interactions
Statement
Reference

sub1794 show allele specific interactions with alleles of ncd. sub1794/+, ncdD/+ transheterozygotes show a high frequency of non-disjunction, whereas either mutation alone is completely recessive. sub1794/+, ncd1/+ transheterozygotes do not show this effect. Df(2R)PC4/+, ncdD/+ shows less nondisjunction than sub1794/+, ncdD/+. sub1794 show a less strong, but still significant, interaction with nod4 than with ncdD. Stage 14 oocytes of sub1794/+, ncdD/+ show a defect in spindle pole formation - most often spindles are either monopolar or tripolar, karyosome may be split, spindles broken or misshapen such that they fray, or do not taper at the poles. Double mutants of sub1794 with ncd1 show no greater degree of oocyte spindle pole abnormality that those of ncd1 mutants alone - the effects of the two mutants are simply additive.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Allelic series (strongest to weakest phenotype): sub131/subDub = sub202/subDub = sub218/subDub = sub22/subDub = sub72/subDub = sub104/subDub = sub158/subDub > sub1794/subDub > +/subDub.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (4)