Allele Dmel\sub¹⁷⁹⁴

General Information
Symbol	Dmel\sub¹⁷⁹⁴	Species	D. melanogaster
Name		FlyBase ID	FBal0135614
Feature type	allele	Associated gene	Dmel\sub

Map ( GBrowse )
Allele class	hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Giunta et al., 2002)
Mutagen	ethyl methanesulfonate (Giunta et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:13,635,029..13,635,029 reported_pr_change=C152Y comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G13635029A pr_change=C152Y\|sub-PA (Giunta et al., 2002)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: C152Y. (Giunta et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	female fertile \| recessive \| reduced (Giunta et al., 2002) female fertile \| reduced (with sub^Dub) (Giunta et al., 2002) female sterile (with sub¹), with Scer\GAL4^{nos.UTR.T:Hsim\VP16}, sub^{CT.Scer\UAS.P\T.T:Avic-EGFP} (Jang et al., 2007) meiotic cell cycle defective (with Df(2R)Pcl^XM82) (Giunta et al., 2002) meiotic cell cycle defective (with sub¹) (Giunta et al., 2002) meiotic cell cycle defective (with sub^Dub) (Giunta et al., 2002) meiotic cell cycle defective \| recessive (Giunta et al., 2002) mitotic cell cycle defective \| maternal effect \| recessive (Giunta et al., 2002)
Phenotype Manifest In
	chromosome, centromeric region (with Df(2R)PC4) (Giunta et al., 2002) karyosome (Giunta et al., 2002) karyosome (with Df(2R)Pcl^XM82) (Giunta et al., 2002) karyosome (with sub^Dub) (Giunta et al., 2002) spindle (Giunta et al., 2002) spindle (with Df(2R)Pcl^XM82) (Giunta et al., 2002) spindle (with sub^Dub) (Giunta et al., 2002)
Detailed Description
	Statement Reference Expression of sub^{CT.Scer\UAS.P\T.T:Avic-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in sub¹⁷⁹⁴/+ females results in reduced numbers of progeny. Expression of sub^{CT.Scer\UAS.P\T.T:Avic-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in sub¹⁷⁹⁴/sub¹ females results in sterility. (Jang et al., 2007) Elevated frequency of X, fourth and second chromosome non-disjunction. Autosomal chromosome loss is significant. Most or all of the non-disjunction involves homologs at meiosis I. The position of the chiasma seems to have little effect on whether the homologs will fail to segregate normally. Achiasmate chromosomes require sub for segregation, whereas chiasmate bivalents can in some instances segregate correctly. Transheterozygotes with sub¹ show elevated levels of X chromosome non-disjunction in females. Stage 14 oocytes of sub¹⁷⁹⁴ homozygotes and transheterozygotes with sub^Dub show a defect in spindle pole formation - most often spindles are either monopolar or tripolar, karyosome may be split, spindles broken or misshapen such that they fray, or do not taper at the poles. When heterozygous with Df(2R)Pcl^XM82 a higher frequency of spindle defects results, though the severity of spindle defects when they occur is unchanged. In stage 14 oocytes of sub¹⁷⁹⁴/Df(2R)PC4 females the centromeres are often abnormally arranged: either on the same side of the karyosome or, if the karyosome is split, in the same mass of chromosomes. Eggs derived from sub¹⁷⁹⁴ mothers show some abnormalities in spindle morphology during early mitoses, though later stage embryos develop normally suggesting recovery from the early defect mitotic defect. (Giunta et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference sub¹⁷⁹⁴ has meiotic cell cycle defective phenotype, enhanceable by ncd^D/ncd[+] (Giunta et al., 2002) sub¹⁷⁹⁴ has meiotic cell cycle defective phenotype, enhanceable by nod[+]/nod⁴ (Giunta et al., 2002)
NOT Enhanced by
Statement Reference sub¹⁷⁹⁴ has meiotic cell cycle defective phenotype, non-enhanceable by ncd[+]/ncd¹ (Giunta et al., 2002)
Enhancer of
Statement Reference sub[+]/sub¹⁷⁹⁴ is an enhancer of meiotic cell cycle defective phenotype of ncd^D (Giunta et al., 2002) sub[+]/sub¹⁷⁹⁴ is an enhancer of meiotic cell cycle defective phenotype of nod⁴ (Giunta et al., 2002)
NOT Enhancer of
Statement Reference sub[+]/sub¹⁷⁹⁴ is a non-enhancer of meiotic cell cycle defective phenotype of ncd¹ (Giunta et al., 2002)
Phenotype Manifest In
Enhanced by
Statement Reference sub¹⁷⁹⁴ has karyosome phenotype, enhanceable by ncd^D/ncd[+] (Giunta et al., 2002) sub¹⁷⁹⁴ has spindle phenotype, enhanceable by ncd^D/ncd[+] (Giunta et al., 2002)
NOT Enhanced by
Statement Reference sub¹⁷⁹⁴ has spindle phenotype, non-enhanceable by ncd¹/ncd¹ (Giunta et al., 2002)
Enhancer of
Statement Reference sub[+]/sub¹⁷⁹⁴ is an enhancer of karyosome phenotype of ncd^D (Giunta et al., 2002) sub[+]/sub¹⁷⁹⁴ is an enhancer of spindle phenotype of ncd^D (Giunta et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference sub¹⁷⁹⁴ show allele specific interactions with alleles of ncd. sub¹⁷⁹⁴/+, ncd^D/+ transheterozygotes show a high frequency of non-disjunction, whereas either mutation alone is completely recessive. sub¹⁷⁹⁴/+, ncd¹/+ transheterozygotes do not show this effect. Df(2R)PC4/+, ncd^D/+ shows less nondisjunction than sub¹⁷⁹⁴/+, ncd^D/+. sub¹⁷⁹⁴ show a less strong, but still significant, interaction with nod⁴ than with ncd^D. Stage 14 oocytes of sub¹⁷⁹⁴/+, ncd^D/+ show a defect in spindle pole formation - most often spindles are either monopolar or tripolar, karyosome may be split, spindles broken or misshapen such that they fray, or do not taper at the poles. Double mutants of sub¹⁷⁹⁴ with ncd¹ show no greater degree of oocyte spindle pole abnormality that those of ncd¹ mutants alone - the effects of the two mutants are simply additive. (Giunta et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	sub¹ (Giunta et al., 2002) sub^Dub (Giunta et al., 2002)
Rescued by	sub^unspecified/sub¹⁷⁹⁴ is rescued by sub^{Scer\UAS.P\T.T:Avic-EGFP}/Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Jang et al., 2007)
Not rescued by	sub^unspecified/sub¹⁷⁹⁴ is not rescued by sub^{CT.Scer\UAS.P\T.T:Avic-EGFP}/Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Jang et al., 2007)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Allelic series (strongest to weakest phenotype): sub¹³¹/sub^Dub = sub²⁰²/sub^Dub = sub²¹⁸/sub^Dub = sub²²/sub^Dub = sub⁷²/sub^Dub = sub¹⁰⁴/sub^Dub = sub¹⁵⁸/sub^Dub > sub¹⁷⁹⁴/sub^Dub > +/sub^Dub. (Giunta et al., 2002)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	sub¹⁷⁹⁴ (Jang et al., 2005, Jang et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Jang et al., 2007, Genetics 177(1): 267--280 Misregulation of the kinesin-like protein subito induces meiotic spindle formation in the absence of chromosomes and centrosomes. [FBrf0201205] Jang et al., 2005, Mol. Biol. Cell 16(10): 4684--4694 The kinesinlike protein Subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. [FBrf0188037] Giunta et al., 2002, Genetics 160(4): 1489--1501 subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogaster. [FBrf0147060]
Review	McKim et al., 2002, A. Rev. Genet. 36: 205--232 Meiotic recombination and chromosome segregation in Drosophila females. [FBrf0151729]

Allele Dmel\sub1794

Allele Dmel\sub¹⁷⁹⁴