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General Information
Symbol
Dmel\sub131
Species
D. melanogaster
Name
FlyBase ID
FBal0135617
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion from site of P{EP}subEP616 (located 105bp upstream of the sub ATG) removing P{EP}subEP616 and more than half of the sub transcription unit.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

sub1/sub131 oocytes exhibit frequent monopolar and tripolar spindles as well as mono-orientation of homologous centromeres while they do not exhibit any failure to separate chromosomes or a defect in the movement of centromeres toward a pole present in the spindle as compared to controls.

The percentage of cells in mitosis in sub1/sub131 mutant larval brains is approximately double that of wild-type.

sub1/sub131 larval brains show an increased frequency of spindle assembly defects. These include frayed microtubules, unequal distribution of microtubules in the two half spindles, and disorganised or absent interpolar microtubules. 70% of mutant brains show disorganised metaphase, and 46% show lagging chromosomes at anaphase compared to 11% and 9% respectively in wild-type brains.

sub131 mutant larval brains show a higher percentage of cells in mitosis (2.66) compared to wild-type brains (1.32).

43% of sub131 mutant brains show disorganised metaphase spindles (including frayed microtubules, unequal distribution of microtubules in the two half spindles, and disorganised or absent interpolar microtubules) compared to 11% of metaphase spindles in wild-type brains. 52% show lagging chromosomes at anaphase compared to 9% in wild-type brains.

14/17 sub1/sub131 oocytes have abnormal spindle organisation. Among the abnormal spindles, 3 are monopolar, 9 are tripolar and 2 have other defects such as fraying of microtubules.

Most mutant oocyte spindles are not bipolar. All mutant spindles, even those that are bipolar, lack the prominent bundles of central spindle microtubules that are seen in wild-type oocytes.

Sterile females show rough eyes and clipped wings. Mutant males show elevated levels of X-Y chromosome non-disjunction in male meiosis. Embryos derived from mutant mothers show early mitotic abnormalities including arrest before embryonic mitoses begin, a polar body near one end and the development of one or two spindles in the middle. Structure of spindles in the embryos is abnormal, and their size variable.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference

sub131/sub1 has brain | larval stage phenotype, enhanceable by polo16-1/polo[+]

Suppressed by
Statement
Reference

sub131 has brain | larval stage phenotype, suppressible by BubR1k03113

sub131/sub1 has brain | larval stage phenotype, suppressible by ncd1/ncd1

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

sub131, BubR1k03113 double mutant larval brains show a lower percentage of cells in mitosis (0.65) compared to sub131 mutant brains (2.66) or wild-type brains (1.32).

sub131, BubR1k03113 double mutant larval brains are slow growing, small, and contain fewer cells than BubR1k03113 mutants.

sub1/sub131; ncd1/ncd1 mutant larval brains show a comparable percentage of cells in mitosis compared to wild-type.

sub1/sub131; polo16-1/+ rare survivors are sickly and small with abdominal patterning defects. Late larval developmental arrest is observed. Severe defects in spindle organization are observed, and spindles are unusually short. Larvae show a higher percentage of cells in mitosis compared to sub1/sub131 or polo16-1/+, and nearly four times that of wild-type.

sub1/sub131, IncenpEC3747/+ mutant larval brains display defects in spindle assembly, and short and disorganised metaphase spindles are common. Anaphase spindles usually lack an organised midzone, and 76% show lagging chromosomes at anaphase (compared to 55% in sub1/sub131 mutant brains). Double mutant larvae exhibit a high frequency of polyploid cells.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Allelic series (strongest to weakest phenotype): sub131/subDub = sub202/subDub = sub218/subDub = sub22/subDub = sub72/subDub = sub104/subDub = sub158/subDub > sub1794/subDub > +/subDub.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)