Allele Dmel\homer^R102

General Information
Symbol	Dmel\homer^R102	Species	D. melanogaster
Name		FlyBase ID	FBal0135705
Feature type	allele	Associated gene	Dmel\homer

Allele class	amorphic allele - genetic evidence, loss of function allele
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - genetic evidence (Naidoo et al., 2012) loss of function allele (Diagana et al., 2002)
Mutagen	Delta2-3 (Diagana et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EP}homer^EP2141 (Diagana et al., 2002)
Nature of the lesion	Statement Reference Deletion of 1.5kb removing from site of P{EP}EP2141 insertion, 60bp upstream of first homer exon, to halfway through the third homer exon. First 168 coding exons of homer are deleted. (Diagana et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	chemical sensitive (Urizar et al., 2007) courtship behavior defective \| male \| recessive (Diagana et al., 2002) fertile (Diagana et al., 2002) hyperactive \| recessive (Diagana et al., 2002) sleep defective (Naidoo et al., 2012) sleep defective (with Df(2L)ED7007) (Naidoo et al., 2012) smell perception defective \| recessive (Diagana et al., 2002) stress response defective (Urizar et al., 2007) viable (Diagana et al., 2002)
Phenotype Manifest In

Detailed Description
	Statement Reference Mutant flies have a normal circadian period and are rhythmic under conditions of constant darkness. Homozygous and homer[R102]/Df(2L)ED7007 flies show fragmentation of sleep compared to wild type. The average bout duration of sleep is significantly reduced in the mutant flies during both night and day periods. The total amount of sleep during the day is not significantly different from that of wild type, but is reduced at night in the mutant flies. Mutant and wild-type flies show recovery sleep immediately following the end of sleep deprivation. The immediate response in the first 4 hours after the end of sleep deprivation is similar in both mutant and wild type. However, in the mutant flies, there is continued rebound sleep over the entire 24 hours after the end of sleep deprivation, while in the wild-type flies the recovery sleep is relatively short. The mutant flies show no increase in average sleep bout duration following 4 or 6 hours of sleep deprivation, but increase their sleep by increasing the number of sleep bouts. This contrasts with wild-type flies which show a significant increase in average sleep bout duration following 4 or 6 hours of sleep deprivation. (Naidoo et al., 2012) homer[R102] mutant male flies exposed to ethanol vapor (56%) for 50 minutes exhibit a mean onset of sedation approximately 5 minutes earlier than in control flies. Furthermore, a larger fraction of homer[R102] mutant flies compared with control flies are sedated at each subsequent time point. homer[R102] heterozygous flies display ethanol sensitivity that is indistinguishable from controls. homer[R102] mutant male flies develop significantly less ethanol tolerance than controls. Whereas only 45% of homer[R102] flies develop rapid tolerance, 64% of controls do. homer[R102] heterozygous males display ethanol tolerance that is intermediate between wild-type and homer[R102] homozygous males. homer[R102] mutants do not display differences to wild-type in ethanol absorption or metabolism. (Urizar et al., 2007) Flies do not display obvious uncoordinated phenotype and can respond to visual stimuli, eliciting an escape response. No anatomical defects are evident and the nervous system morphology and development appears normal. Mutant males show behavioral plasticity deficits and fail to form and/or retain conditioning by the non-receptive mated female in courtship conditioning assays. Mutants do suppress courtship behavior during conditioning but show higher levels of courtship than wild type both before and after conditioning. Response to propionic acid (measured by chemosensory jump response) is similar to that of wild type, response to benzaldehyde is elevated, compared to wild type and mutant flies show a higher level of locomotor activity than wild type. (Diagana et al., 2002)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	homer^R102 is rescued by homer^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^c819 (Urizar et al., 2007) homer^R102 is rescued by Scer\GAL4^c42/homer^{Scer\UAS.T:Hsap\MYC} (Urizar et al., 2007) homer^R102 is rescued by Scer\GAL4^elav-C155/homer^{Scer\UAS.T:Hsap\MYC} (Urizar et al., 2007)
Not rescued by	homer^R102 is not rescued by homer^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^103Y (Urizar et al., 2007) homer^R102 is not rescued by homer^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4²⁴⁷ (Urizar et al., 2007) homer^R102 is not rescued by homer^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^c107 (Urizar et al., 2007) homer^R102 is not rescued by homer^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^c302 (Urizar et al., 2007) homer^R102 is not rescued by homer^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^Ilp2.PR (Urizar et al., 2007) homer^R102 is not rescued by homer^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^lilli-189Y (Urizar et al., 2007) homer^R102 is not rescued by Scer\GAL4^078Y/homer^{Scer\UAS.T:Hsap\MYC} (Urizar et al., 2007) homer^R102 is not rescued by Scer\GAL4^121Y/homer^{Scer\UAS.T:Hsap\MYC} (Urizar et al., 2007) homer^R102 is not rescued by Scer\GAL4^Aph-4-c232/homer^{Scer\UAS.T:Hsap\MYC} (Urizar et al., 2007) homer^R102 is not rescued by Scer\GAL4^c105/homer^{Scer\UAS.T:Hsap\MYC} (Urizar et al., 2007) homer^R102 is not rescued by Scer\GAL4^c481/homer^{Scer\UAS.T:Hsap\MYC} (Urizar et al., 2007) homer^R102 is not rescued by Scer\GAL4^c522/homer^{Scer\UAS.T:Hsap\MYC} (Urizar et al., 2007)
Comments	Pan-neuronal expression of homer[Scer\UAS.T:Hsap\MYC] restores the ethanol sensitivity and rapid tolerance phenotypes of homer[R102] homozygous males to control levels. Expression of homer[Scer\UAS.T:Hsap\MYC] in various subsections of the brain under the control of Scer\GAL4[c522], Scer\GAL4[121Y], Scer\GAL4[247], Scer\GAL4[103Y], Scer\GAL4[c107], Scer\GAL4[Ilp2.PR], Scer\GAL4[078Y], Scer\GAL4[Aph-4-c232], Scer\GAL4[c105], Scer\GAL4[c481], Scer\GAL4[lilli-189Y] or Scer\GAL4[c302] does not affect ethanol sensitivity in homer[R102] mutant male flies. Expression of homer[Scer\UAS.T:Hsap\MYC] in the R2 and R4m Ring neurons of the ellipsoid body under the control of Scer\GAL4[c819] or Scer\GAL4[c42] results in significant rescue of the homer[R102] ethanol sensitivity and rapid tolerance phenotype. (Urizar et al., 2007)
Stocks ( 1 )
Bloomington	9564 homer^R102
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	homer^R102 (Urizar et al., 2007, Naidoo et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Naidoo et al., 2012, PLoS ONE 7(4): e35174 Role of homer proteins in the maintenance of sleep-wake States. [FBrf0218096] Urizar et al., 2007, J. Neurosci. 27(17): 4541--4551 Drosophila Homer is required in a small set of neurons including the ellipsoid body for normal ethanol sensitivity and tolerance. [FBrf0201158] Diagana et al., 2002, J. Neurosci. 22(2): 428--436 Mutation of Drosophila homer disrupts control of locomotor activity and behavioral plasticity. [FBrf0141617]
Abstract	Urizar et al., 2006, A. Dros. Res. Conf. 47: 42 Drosophila Homer Is Required in Neurons of the Ellipsoid Body and Pars Intercerebralis for Normal Ethanol Sensitivity and Tolerance. [FBrf0189089]

Allele Dmel\homerR102

Allele Dmel\homer^R102