FB2025_01 , released February 20, 2025
Allele: Dmel\WASpH242D.UAS
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General Information
Symbol
Dmel\WASpH242D.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0135763
Feature type
allele
Associated gene
Dmel\WASp
Associated Insertion(s)
Carried in Construct
P{UAS-WASp.H242D}
Also Known As
WSPH242D
Key Links
Transgenic product class
missense_variant
(Tal et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Tal et al., 2002)
Mutagen
in vitro construct
(Tal et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-WASp.H242D}
Cytology
Description

UASt regulatory sequences drive expression of full-length WASp in which a crucial His residue within the GTPase-binding domain has been replaced with an Asp residue.

(Tal et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
WASp
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing WASpH242D.Scer\UAS under the control of Scer\GAL4arm.PS in a WASp1/Df(3R)3450 mutant background display similarly elongated cysts to wild type, but no sperm is released into the seminal vesicle. Coiling of the cysts does not take place, and the extreme basal end of the testis tube, lined by the terminal epithelium and leading up to the seminal vesicle, is free of cysts altogether. Sperm individualisation in these mutants proceeds normally. The microfilaments and nuclei of late-stage cysts show significant structural alterations. There is a consistent failure to achieve the intertwined and tightly packed organisation of head cyst cell F-actin arrays and associated spermatid nuclei. The microfilament arrays appear sparse and misshapen, and the normally tight and uniformly oriented bundles of nuclei are loosely packed and partially split.

      (Rotkopf et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      WASpH242D.UAS/Scer\GAL4nSyb.PS rescues Df(3R)3450/WASp1

      (Khuong et al., 2010)

      Scer\GAL4arm.PS/WASpH242D.UAS rescues Df(3R)3450/WASp1

      (Tal et al., 2002)
      Comments

      Expression of WASpH242D.Scer\UAS under the control of Scer\GAL4n-syb.PS rescues the neuromuscular junction overgrowth seen in WASp1/Df(3R)3450 third instar larvae.

      (Khuong et al., 2010)

      The addition of WASpH242D.Scer\UAS driven by Scer\GAL4arm.PS to WASp1/Df(3R)3450 flies, rescues the bristle phenotype seen in these animals.

      (Tal et al., 2002)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      WASpH242D.Scer\UAS
      WASpH242D.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)