FlyBase Allele Report: Dmel\Trxr1[mito.UAS]

General Information

Symbol

Dmel\Trxr1^mito.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0135773

Feature type

allele

Associated gene

Dmel\Trxr1

Associated Insertion(s)

Carried in Construct

P{UAS-Trxr1.mito}

Key Links

Transgenic product class

wild_type

(Missirlis et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Missirlis et al., 2002)

Mutagen

in vitro construct

(Missirlis et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Trxr1.mito}

Cytology

Description

UASt regulatory sequences drive expression of the mitochondrial isoform of Trxr-1.

(Missirlis et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Trxr1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^VGlut1-OK371

(Smith et al., 2019)

Phenotype Manifest In

axon | adult stage, with Scer\GAL4^VGlut1-OK371

(Smith et al., 2019)

mitochondrion | adult stage, with Scer\GAL4^VGlut1-OK371

(Smith et al., 2019)

Detailed Description

Statement

Reference

Expression of Trxr-1^mito.UAS under the control of Scer\GAL4^VGlut-OK371 significantly reduces mitochondrial number in axons of adult neurons, compared to controls, with no effect on mitochondrial length.

(Smith et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^VGlut1-OK371, Trxr1^mito.UAS has abnormal neuroanatomy | adult stage phenotype, non-enhanceable | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

NOT suppressed by

Statement

Reference

Scer\GAL4^VGlut1-OK371, Trxr1^mito.UAS has abnormal neuroanatomy | adult stage phenotype, non-suppressible | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Suppressor of

Statement

Reference

Scer\GAL4^VGlut1-OK371/Trxr1^mito.UAS is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^VGlut1-OK371, Trxr1^mito.UAS has mitochondrion | adult stage phenotype, non-enhanceable | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Scer\GAL4^VGlut1-OK371, Trxr1^mito.UAS has axon | adult stage phenotype, non-enhanceable | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

NOT suppressed by

Statement

Reference

Scer\GAL4^VGlut1-OK371, Trxr1^mito.UAS has mitochondrion | adult stage phenotype, non-suppressible | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Scer\GAL4^VGlut1-OK371, Trxr1^mito.UAS has axon | adult stage phenotype, non-suppressible | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Suppressor of

Statement

Reference

Scer\GAL4^VGlut1-OK371/Trxr1^mito.UAS is a suppressor of mitochondrion | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Scer\GAL4^VGlut1-OK371/Trxr1^mito.UAS is a suppressor of axon | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Trxr1^cyto.UAS, Scer\GAL4^Act5C.PI, Trxr1^mito.UAS rescues Trxr1^Δ1

(Missirlis et al., 2002)

Scer\GAL4^Act5C.PI/Trxr1^mito.UAS rescues Trxr1^Δ2

(Missirlis et al., 2002)

Scer\GAL4^Act5C.PI/Trxr1^mito.UAS rescues Trxr1^G0477

(Missirlis et al., 2002)

Partially rescues

Scer\GAL4^Act5C.PI/Trxr1^mito.UAS partially rescues Trxr1^Δ1

(Missirlis et al., 2002)

Fails to rescue

Scer\GAL4^Act5C.PI/Trxr1^mito.UAS fails to rescue Trxr1^G0481

(Missirlis et al., 2002)

Comments

Expression of Trxr-1^{mito.Scer\UAS} under the control of Scer\GAL4^Act5C.PI restores the lifespan of Trxr-1^G0477 hemizygous males from a few days up to the range of the wild-type lifespan. Expression of Trxr-1^{mito.Scer\UAS} under the control of Scer\GAL4^Act5C.PI restores the lifespan of Trxr-1^Δ2 hemizygous males from a few days up to the range of the wild-type lifespan. Expression of Trxr-1^{mito.Scer\UAS} under the control of Scer\GAL4^Act5C.PI partially rescues the pre-adult lethality of Trxr-1^Δ1 flies and also increases the lifespan of the rescued adults to about half that of the lifespan of wild-type flies. Expression of both Trxr-1^{mito.Scer\UAS} and Trxr-1^{cyto.Scer\UAS} under the control of Scer\GAL4^Act5C.PI rescues the lifespan of Trxr-1^Δ1 flies to normal.

(Missirlis et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Trxr-1^{mito.Scer\UAS}

Trxr-1^mito.UAS

Trxr1^mito.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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