Allele Dmel\Rac1^J11

General Information
Symbol	Dmel\Rac1^J11	Species	D. melanogaster
Name		FlyBase ID	FBal0135835
Feature type	allele	Associated gene	Dmel\Rac1

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms male semi-sterile
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Hakeda-Suzuki et al., 2002, Ng et al., 2002, Stramer et al., 2005, Woolner et al., 2005)
Mutagen	ethyl methanesulfonate (Ng et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:1,302,168..1,302,168 pr_change=G60E\|Rac1-PA evidence=experimental comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. reported_pr_change=G?E na_change=G1302168A (Ng et al., 2002)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: G60E. (Ng et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective \| larval stage (Sampson et al., 2012) male semi-sterile (Raymond et al., 2004) neuroanatomy defective (Ng et al., 2002) neuroanatomy defective \| dominant (Reeve et al., 2005, Higuchi et al., 2009) neuroanatomy defective \| somatic clone (Ng et al., 2002, Srahna et al., 2006)
Phenotype Manifest In
	adult mushroom body (Ng et al., 2002) adult mushroom body (with Df(3L)Rac1) (Ng et al., 2002) adult mushroom body \| somatic clone (Ng et al., 2002) axon & dorsal cluster neuron \| somatic clone (Srahna et al., 2006) dendritic arborizing neuron (Lee et al., 2003) dorsal multidendritic neuron ddaC (Lee et al., 2003) LNv neuron (Reeve et al., 2005) lobe system of mushroom body (Higuchi et al., 2009) peripheral glial cell (Sepp and Auld, 2003) presumptive embryonic salivary gland (Pirraglia et al., 2006) ventral adult lateral neuron & commissure (Reeve et al., 2005)
Detailed Description
	Statement Reference Homozygous larvae show a strongly reduced rate of encapsulation (11%) of eggs of the avirulent wasp strain L. boulardi G486 compared to the rate seen in wild-type larvae. (Sampson et al., 2012) 65% of heterozygotes have normal alpha/beta mushroom body lobes and 35% have branching defects in the lobes. (Higuchi et al., 2009) Heterozygous and Rac1[J11]/Rac1[J10] flies show normal resistance to infection with P.aeruginosa by septic injury. (Avet-Rochex et al., 2007) The salivary gland cells invaginate in Rac1[J11] mutant embryos, but the gland does not complete its posterior migration, but instead is abnormally shaped and appears attached to the ventral surface. (Pirraglia et al., 2006) In contrast to wild-type animals, where only 38% of dorsal cluster neuron axons innervate the medulla, 87% of axons from Rac1^J11 neuron clones innervate this region. (Srahna et al., 2006) An ectopic collateral branch is observed on the small LNv projections in 17% of Rac1^J11/+ adult brains. The posterior tract of the LNv neurons shows a defasciculation phenotype in 33% of brains. (Reeve et al., 2005) 46% of homozygous males are sterile in individual crosses with wild-type females, although the testes appear normal and the seminal vesicle is full of motile sperm. (Raymond et al., 2004) No gross defects are seen in dendritic branching patterns are seen in later embryogenesis stages. however, when single cell mutant clones are made during larval stages, dendritic arborising neurons (DA neurons) exhibit fewer dendritic branches than wild-type. ddaC neurons exhibit a 23% reduction in the number of dendritic branches. (Lee et al., 2003) Homozygous mutant embryos show lack of peripheral glial coverage of lateral axon tracts and abnormal glial profiles are seen in the ventral region. The motor neuron projections and the lateral chordotonal sensory neurons are sometimes seen to be stalled. (Sepp and Auld, 2003) Heterozygous adults show significant defects in branching of the mushroom body axons and some guidance defects in the mushroom body axons. Homozygotes show mainly guidance defects in the mushroom body axons. In mosaic animals, 21% of neuroblast clones show defective guidance. (Ng et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference DAAM^Ex68, Rac1^J11, Rac2^Δ has neuroanatomy defective phenotype, enhanceable by Mtl^Δ (Matusek et al., 2008) DAAM^Ex68, Rac1^J11 has neuroanatomy defective phenotype, enhanceable by Rac2^Δ (Matusek et al., 2008) Mtl^Δ, Rac1^J11, Rac2^Δ has planar polarity defective \| somatic clone phenotype, enhanceable by Cdc42⁵ (Muñoz-Descalzo et al., 2007) Rac1^J11 has male semi-sterile phenotype, enhanceable by cdi^R47/cdi[+] (Raymond et al., 2004) Rac1^J11 has neuroanatomy defective phenotype, enhanceable by Dp(2;2)C619/+ (Reeve et al., 2005) Rac1^J11 has neuroanatomy defective phenotype, enhanceable by Fmr1^Δ113M/Fmr1[+] (Reeve et al., 2005)
NOT Enhanced by
Statement Reference GluRIIA^SP16, Rac1^J11/Rac1[+] has neurophysiology defective \| recessive \| third instar larval stage phenotype, non-enhanceable by Exn^EY01953/Exn[+] (Frank et al., 2009)
Suppressed by
Statement Reference Mtl^Δ, Rac1^J11, Rac2^Δ has lethal \| embryonic stage phenotype, suppressible \| partially by hep^CA.Scer\UAS/Scer\GAL4[-] (Woolner et al., 2005)
Enhancer of
Statement Reference Rac1^J11, Rac2^Δ, Mtl[+], Mtl^Δ, Rac2[+], Rac1[+] is an enhancer of planar polarity defective phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, cindr^{dsRNA.PC.PD.Scer\UAS} (Johnson et al., 2008) Rac1^J11, Rac2^Δ, Mtl^Δ is an enhancer of neuroanatomy defective phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11/Rac1[+] is an enhancer of neuroanatomy defective phenotype of Fmr1^Δ113M (Reeve et al., 2005) Rac1^J11/Rac1[+] is an enhancer of neuroanatomy defective phenotype of LIMK1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^ey-OK107 (Ng and Luo, 2004) Rac1^J11/Rac1[+] is an enhancer of neuroanatomy defective phenotype of PsGEF^Δ21 (Higuchi et al., 2009) Rac1^J11/Rac1[+] is an enhancer of planar polarity defective phenotype of Mtl^Scer\UAS.cMa, Scer\GAL4^hs.2sev (Muñoz-Descalzo et al., 2007) Rac1^J11/Rac2^Δ is an enhancer of neuroanatomy defective phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11 is an enhancer of neuroanatomy defective phenotype of DAAM^Ex68 (Matusek et al., 2008)
NOT Enhancer of
Statement Reference Rac1^J11, Rac2^Δ, Mtl[+], Mtl^Δ, Rac2[+], Rac1[+] is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) Rac1^J11, Rac2^Δ, Mtl^Δ is a non-enhancer of neuroanatomy defective phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11, Rac2^Δ, Mtl^Δ is a non-enhancer of neuroanatomy defective phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, msn^EP549 (Ruan et al., 2002) Rac1^J11, Rac2^Δ, Rac2[+], Rac1[+] is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) Rac1^J11/Rac1[+] is a non-enhancer of visible \| dominant phenotype of br¹ (Chen et al., 2004) Rac1^J11/Rac1[+] is a non-enhancer of visible \| dominant phenotype of Sb^63b (Chen et al., 2004) Rac1^J11/Rac1[+] is a non-enhancer of visible \| dominant phenotype of Sb⁷⁰ (Chen et al., 2004) Rac1^J11/Rac1^J10 is a non-enhancer of neuroanatomy defective phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11/Rac2^Δ is a non-enhancer of neuroanatomy defective phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11 is a non-enhancer of neuroanatomy defective phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008)
NOT Suppressor of
Statement Reference Rac1^J11, Rac2^Δ, Mtl[+], Mtl^Δ, Rac2[+], Rac1[+] is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) Rac1^J11, Rac2^Δ, Mtl^Δ is a non-suppressor of neuroanatomy defective phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, msn^EP549 (Ruan et al., 2002) Rac1^J11, Rac2^Δ, Rac2[+], Rac1[+] is a non-suppressor of visible phenotype of HIV-1\Vpu^Scer\UAS.cLa, Scer\GAL4^dpp.blk1 (Marchal et al., 2012) Rac1^J11, Rac2^Δ, Rac2[+], Rac1[+] is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) Rac1^J11/Rac1[+] is a non-suppressor of visible phenotype of HIV-1\Vpu^Scer\UAS.cLa, Scer\GAL4^dpp.blk1 (Marchal et al., 2012)
Other
Statement Reference GluRIIA^SP16, Rac1^J11/Rac1[+], cac^S/cac[+] has neurophysiology defective \| recessive \| third instar larval stage phenotype (Frank et al., 2009) GluRIIA^SP16, Rac1^J11/Rac1[+] has neurophysiology defective \| recessive \| third instar larval stage phenotype (Frank et al., 2009) Mtl[+]/Mtl^Δ, Rac1^J11, Rac2^Δ/Rac2[+] has neuroanatomy defective phenotype (Ng et al., 2002) Mtl[+]/Mtl^Δ, Rac1^J11, Rac2^Δ has neuroanatomy defective \| somatic clone phenotype (Ng et al., 2002, Ng et al., 2002, Ng et al., 2002) Mtl[+]/Mtl^Δ, Rac1^J11/Rac1[+], Rac2^Δ has neuroanatomy defective phenotype (Ng et al., 2002) Mtl^Δ, Rac1^J11, Rac2^Δ/Rac2[+] has neuroanatomy defective phenotype (Ng et al., 2002) Mtl^Δ, Rac1^J11, Rac2^Δ has lethal \| embryonic stage phenotype (Woolner et al., 2005, Woolner et al., 2005, Woolner et al., 2005) Mtl^Δ, Rac1^J11, Rac2^Δ has lethal \| germline clone \| embryonic stage phenotype (Woolner et al., 2005, Woolner et al., 2005, Woolner et al., 2005) Mtl^Δ, Rac1^J11, Rac2^Δ has neuroanatomy defective \| somatic clone phenotype (Ng et al., 2002, Ng et al., 2002, Ng et al., 2002, Ng et al., 2002, Ng et al., 2002) Mtl^Δ, Rac1^J11, Rac2^Δ has neuroanatomy defective phenotype (Pinal and Pichaud, 2011) Mtl^Δ, Rac1^J11, Rac2^Δ has planar polarity defective \| somatic clone phenotype (Muñoz-Descalzo et al., 2007) Mtl^Δ, Rac1^J11/Rac1[+], Rac2^Δ/Rac2[+] has neuroanatomy defective phenotype (Ng et al., 2002) Mtl^Δ, Rac1^J11/Rac1[+], Rac2^Δ has neuroanatomy defective phenotype (Ng et al., 2002) Rac1^J11, Rac2^Δ/Rac2[+] has neuroanatomy defective phenotype (Rodriguez Moncalvo and Campos, 2005, Ng et al., 2002) Rac1^J11, Rac2^Δ has neuroanatomy defective \| somatic clone phenotype (Ng et al., 2002, Ng et al., 2002) Rac1^J11, Rac2^Δ has neuroanatomy defective phenotype (Rodriguez Moncalvo and Campos, 2005, Rodriguez Moncalvo and Campos, 2005, Ng et al., 2002) Rac1^J11, sli² has neuroanatomy defective phenotype (Fan et al., 2003) Rac1^J11/Df(3L)Rac1, Rac2^Δ/Rac2[+] has viable phenotype (Ng et al., 2002) Rac1^J11/Df(3L)Rac1, Rac2^Δ has lethal phenotype (Ng et al., 2002, Ng et al., 2002) Rac1^J11/Df(3L)Rac1, Rac2^Δ has viable phenotype (Ng et al., 2002) Rac1^J11/Rac1[+], Rac2^Δ has neuroanatomy defective phenotype (Rodriguez Moncalvo and Campos, 2005)
Phenotype Manifest In
Enhanced by
Statement Reference DAAM^Ex68, Rac1^J11, Rac2^Δ has connective phenotype, enhanceable by Mtl^Δ (Matusek et al., 2008) DAAM^Ex68, Rac1^J11, Rac2^Δ has embryo phenotype, enhanceable by Mtl^Δ (Matusek et al., 2008) DAAM^Ex68, Rac1^J11, Rac2^Δ has fascicle phenotype, enhanceable by Mtl^Δ (Matusek et al., 2008) DAAM^Ex68, Rac1^J11, Rac2^Δ has lateral longitudinal fascicle phenotype, enhanceable by Mtl^Δ (Matusek et al., 2008) DAAM^Ex68, Rac1^J11, Rac2^Δ has neuropil phenotype, enhanceable by Mtl^Δ (Matusek et al., 2008) DAAM^Ex68, Rac1^J11 has connective phenotype, enhanceable by Rac2^Δ (Matusek et al., 2008) DAAM^Ex68, Rac1^J11 has embryo phenotype, enhanceable by Rac2^Δ (Matusek et al., 2008) DAAM^Ex68, Rac1^J11 has fascicle phenotype, enhanceable by Rac2^Δ (Matusek et al., 2008) DAAM^Ex68, Rac1^J11 has lateral longitudinal fascicle phenotype, enhanceable by Rac2^Δ (Matusek et al., 2008) DAAM^Ex68, Rac1^J11 has neuropil phenotype, enhanceable by Rac2^Δ (Matusek et al., 2008) Mtl^Δ, Rac1^J11, Rac2^Δ has ommatidium \| somatic clone phenotype, enhanceable by Cdc42⁵ (Muñoz-Descalzo et al., 2007) Rac1^J11, Rac2^Δ/Rac2[+] has dorsal group branch precursor phenotype, enhanceable by bnl[+]/bnl⁰⁰⁸⁵⁷ (Chihara et al., 2003) Rac1^J11, Rac2^Δ/Rac2[+] has dorsal group branch precursor phenotype, enhanceable by btl[+]/btl^ΔOh10 (Chihara et al., 2003) Rac1^J11, Rac2^Δ/Rac2[+] has dorsal group branch precursor phenotype, enhanceable by stumps^09904b/stumps[+] (Chihara et al., 2003) Rac1^J11, Rac2^Δ/Rac2[+] has dorsal trunk primordium phenotype, enhanceable by bnl[+]/bnl⁰⁰⁸⁵⁷ (Chihara et al., 2003) Rac1^J11, Rac2^Δ/Rac2[+] has dorsal trunk primordium phenotype, enhanceable by btl[+]/btl^ΔOh10 (Chihara et al., 2003) Rac1^J11, Rac2^Δ/Rac2[+] has dorsal trunk primordium phenotype, enhanceable by stumps^09904b/stumps[+] (Chihara et al., 2003) Rac1^J11, Rac2^Δ/Rac2[+] has tracheal branch primordium phenotype, enhanceable by bnl[+]/bnl⁰⁰⁸⁵⁷ (Chihara et al., 2003) Rac1^J11, Rac2^Δ/Rac2[+] has tracheal branch primordium phenotype, enhanceable by btl[+]/btl^ΔOh10 (Chihara et al., 2003) Rac1^J11, Rac2^Δ/Rac2[+] has tracheal branch primordium phenotype, enhanceable by stumps^09904b/stumps[+] (Chihara et al., 2003) Rac1^J11 has LNv neuron phenotype, enhanceable by Dp(2;2)C619/+ (Reeve et al., 2005) Rac1^J11 has LNv neuron phenotype, enhanceable by Fmr1^Δ113M/Fmr1[+] (Reeve et al., 2005) Rac1^J11 has presumptive embryonic salivary gland phenotype, enhanceable by Rac2^Δ (Pirraglia et al., 2006) Rac1^J11 has ventral adult lateral neuron & commissure phenotype, enhanceable by Dp(2;2)C619/+ (Reeve et al., 2005) Rac1^J11 has ventral adult lateral neuron & commissure phenotype, enhanceable by Fmr1^Δ113M/Fmr1[+] (Reeve et al., 2005)
NOT Enhanced by
Statement Reference GluRIIA^SP16, Rac1^J11/Rac1[+] has embryonic/larval neuromuscular junction phenotype, non-enhanceable by Exn^EY01953/Exn[+] (Frank et al., 2009)
Suppressed by
Statement Reference Mtl^Δ, Rac1^J11, Rac2^Δ has dorsal closure embryo phenotype, suppressible \| partially by hep^CA.Scer\UAS/Scer\GAL4[-] (Woolner et al., 2005) Mtl^Δ, Rac1^J11, Rac2^Δ has embryonic/first instar larval cuticle phenotype, suppressible \| partially by hep^CA.Scer\UAS/Scer\GAL4[-] (Woolner et al., 2005) Rac1^J11, Rac2^Δ has dorsal closure embryo phenotype, suppressible by cv-c^M62 (Denholm et al., 2005) Rac1^J11, Rac2^Δ has ganglionic branch primordium phenotype, suppressible \| partially by RhoGAP93B¹ (Lundstrom et al., 2004) Rac1^J11, Rac2^Δ has ganglionic branch primordium phenotype, suppressible \| partially by robo⁴ (Lundstrom et al., 2004)
Enhancer of
Statement Reference Rac1^J11, Rac2^Δ, Mtl[+], Mtl^Δ, Rac2[+], Rac1[+] is an enhancer of cone cell phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, cindr^{dsRNA.PC.PD.Scer\UAS} (Johnson et al., 2008) Rac1^J11, Rac2^Δ, Mtl[+], Mtl^Δ, Rac2[+], Rac1[+] is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, cindr^{dsRNA.PC.PD.Scer\UAS} (Johnson et al., 2008) Rac1^J11, Rac2^Δ, Mtl[+], Mtl^Δ, Rac2[+], Rac1[+] is an enhancer of pigment cell phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, cindr^{dsRNA.PC.PD.Scer\UAS} (Johnson et al., 2008) Rac1^J11, Rac2^Δ, Mtl^Δ is an enhancer of connective phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11, Rac2^Δ, Mtl^Δ is an enhancer of embryo phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11, Rac2^Δ, Mtl^Δ is an enhancer of fascicle phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11, Rac2^Δ, Mtl^Δ is an enhancer of lateral longitudinal fascicle phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11, Rac2^Δ, Mtl^Δ is an enhancer of neuropil phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11/Rac1[+] is an enhancer of adult mushroom body phenotype of LIMK1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^ey-OK107 (Ng and Luo, 2004) Rac1^J11/Rac1[+] is an enhancer of LNv neuron phenotype of Fmr1^Δ113M (Reeve et al., 2005) Rac1^J11/Rac1[+] is an enhancer of mushroom body phenotype of PsGEF^Δ21 (Higuchi et al., 2009) Rac1^J11/Rac1[+] is an enhancer of ommatidium phenotype of Mtl^Scer\UAS.cMa, Scer\GAL4^hs.2sev (Muñoz-Descalzo et al., 2007) Rac1^J11/Rac1[+] is an enhancer of ventral adult lateral neuron & commissure phenotype of Fmr1^Δ113M (Reeve et al., 2005) Rac1^J11/Rac1[+] is an enhancer of wing hair \| supernumerary phenotype of Scer\GAL4^ap-md544, trc^{T453A.Scer\UAS} (He et al., 2005) Rac1^J11/Rac2^Δ is an enhancer of connective phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11/Rac2^Δ is an enhancer of embryo phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11/Rac2^Δ is an enhancer of fascicle phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11/Rac2^Δ is an enhancer of lateral longitudinal fascicle phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11/Rac2^Δ is an enhancer of neuropil phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11/Rac2^Δ is an enhancer of posterior spiracle primordium phenotype of cv-c^M62 (Denholm et al., 2005) Rac1^J11 is an enhancer of connective phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11 is an enhancer of embryo phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11 is an enhancer of fascicle phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11 is an enhancer of lateral longitudinal fascicle phenotype of DAAM^Ex68 (Matusek et al., 2008) Rac1^J11 is an enhancer of neuropil phenotype of DAAM^Ex68 (Matusek et al., 2008)
NOT Enhancer of
Statement Reference Rac1^J11, Mtl^Δ, Rac1^J10 is a non-enhancer of fascicle \| embryonic stage phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11, Rac2^Δ, Mtl[+], Mtl^Δ, Rac2[+], Rac1[+] is a non-enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) Rac1^J11, Rac2^Δ, Mtl^Δ is a non-enhancer of commissure \| embryonic stage phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11, Rac2^Δ, Mtl^Δ is a non-enhancer of fascicle \| embryonic stage phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11, Rac2^Δ, Mtl^Δ is a non-enhancer of neuropil \| embryonic stage phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11, Rac2^Δ, Mtl^Δ is a non-enhancer of photoreceptor cell & axon phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, msn^EP549 (Ruan et al., 2002) Rac1^J11, Rac2^Δ, Rac2[+], Rac1[+] is a non-enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) Rac1^J11/Mtl^Δ is a non-enhancer of fascicle \| embryonic stage phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11/Rac1[+] is a non-enhancer of leg phenotype of br¹ (Chen et al., 2004) Rac1^J11/Rac1[+] is a non-enhancer of leg phenotype of Sb^63b (Chen et al., 2004) Rac1^J11/Rac1[+] is a non-enhancer of leg phenotype of Sb⁷⁰ (Chen et al., 2004) Rac1^J11/Rac1^J10, Scer\GAL4^elav-C155 is a non-enhancer of neuropil \| embryonic stage phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11/Rac1^J10 is a non-enhancer of commissure \| embryonic stage phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11/Rac2^Δ is a non-enhancer of commissure \| embryonic stage phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11/Rac2^Δ is a non-enhancer of fascicle \| embryonic stage phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11/Rac2^Δ is a non-enhancer of neuropil \| embryonic stage phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11 is a non-enhancer of commissure \| embryonic stage phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008) Rac1^J11 is a non-enhancer of neuropil \| embryonic stage phenotype of DAAM^{C.Scer\UAS.P\T}, Scer\GAL4^elav-C155 (Matusek et al., 2008)
Suppressor of
Statement Reference Rac1^J11, Rac2^Δ, Rac2[+], Rac1[+] is a suppressor of eye phenotype of Ced-12^Scer\UAS.cGa, Scer\GAL4^Mef2.PR, mbc^Scer\UAS.cBa (Geisbrecht et al., 2008) Rac1^J11/Rac1[+] is a suppressor of NMJ bouton phenotype of Vps35^EY14200 (Korolchuk et al., 2007)
NOT Suppressor of
Statement Reference Rac1^J11, Rac2^Δ, Mtl[+], Mtl^Δ, Rac2[+], Rac1[+] is a non-suppressor of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) Rac1^J11, Rac2^Δ, Mtl^Δ is a non-suppressor of photoreceptor cell & axon phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, msn^EP549 (Ruan et al., 2002) Rac1^J11, Rac2^Δ, Rac2[+], Rac1[+] is a non-suppressor of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053 (Djiane and Mlodzik, 2010) Rac1^J11, Rac2^Δ, Rac2[+], Rac1[+] is a non-suppressor of wing phenotype of HIV-1\Vpu^Scer\UAS.cLa, Scer\GAL4^dpp.blk1 (Marchal et al., 2012) Rac1^J11/Rac1[+] is a non-suppressor of wing phenotype of HIV-1\Vpu^Scer\UAS.cLa, Scer\GAL4^dpp.blk1 (Marchal et al., 2012)
Other
Statement Reference GluRIIA^SP16, Rac1^J11/Rac1[+], cac^S/cac[+] has embryonic/larval neuromuscular junction phenotype (Frank et al., 2009) GluRIIA^SP16, Rac1^J11/Rac1[+] has embryonic/larval neuromuscular junction phenotype (Frank et al., 2009) Mtl[+]/Mtl^Δ, Rac1^J11, Rac2^Δ/Rac2[+] has adult mushroom body phenotype (Ng et al., 2002) Mtl[+]/Mtl^Δ, Rac1^J11, Rac2^Δ has adult mushroom body \| somatic clone phenotype (Ng et al., 2002, Ng et al., 2002, Ng et al., 2002) Mtl[+]/Mtl^Δ, Rac1^J11/Rac1[+], Rac2^Δ/Rac2[+], shi² has presumptive embryonic salivary gland \| heat sensitive phenotype (Pirraglia et al., 2006) Mtl[+]/Mtl^Δ, Rac1^J11/Rac1[+], Rac2^Δ has adult mushroom body phenotype (Ng et al., 2002) Mtl^Δ, Rac1^J11, Rac2^Δ/Rac2[+] has adult mushroom body phenotype (Ng et al., 2002) Mtl^Δ, Rac1^J11, Rac2^Δ has adult mushroom body \| somatic clone phenotype (Ng et al., 2002, Ng et al., 2002, Ng et al., 2002, Ng et al., 2002, Ng et al., 2002) Mtl^Δ, Rac1^J11, Rac2^Δ has amnioserosa phenotype (Woolner et al., 2005, Woolner et al., 2005, Woolner et al., 2005) Mtl^Δ, Rac1^J11, Rac2^Δ has dorsal closure embryo phenotype (Woolner et al., 2005, Woolner et al., 2005, Woolner et al., 2005) Mtl^Δ, Rac1^J11, Rac2^Δ has embryonic/first instar larval cuticle phenotype (Woolner et al., 2005, Woolner et al., 2005, Woolner et al., 2005) Mtl^Δ, Rac1^J11, Rac2^Δ has embryonic hemocyte phenotype (Stramer et al., 2005, Stramer et al., 2005, Stramer et al., 2005) Mtl^Δ, Rac1^J11, Rac2^Δ has embryonic leading edge cell & actin filament phenotype (Woolner et al., 2005, Woolner et al., 2005, Woolner et al., 2005) Mtl^Δ, Rac1^J11, Rac2^Δ has embryonic leading edge cell phenotype (Woolner et al., 2005, Woolner et al., 2005, Woolner et al., 2005) Mtl^Δ, Rac1^J11, Rac2^Δ has embryonic myoblast phenotype (Richardson et al., 2007) Mtl^Δ, Rac1^J11, Rac2^Δ has ommatidium \| somatic clone phenotype (Muñoz-Descalzo et al., 2007) Mtl^Δ, Rac1^J11, Rac2^Δ has optic lobe phenotype (Pinal and Pichaud, 2011) Mtl^Δ, Rac1^J11, Rac2^Δ has photoreceptor cell & axon phenotype (Hakeda-Suzuki et al., 2002, Hakeda-Suzuki et al., 2002, Hakeda-Suzuki et al., 2002) Mtl^Δ, Rac1^J11/Rac1[+], Rac2^Δ/Rac2[+] has adult mushroom body phenotype (Ng et al., 2002) Mtl^Δ, Rac1^J11/Rac1[+], Rac2^Δ has adult mushroom body phenotype (Ng et al., 2002) Rac1^J11, cdi^R47/cdi[+] has testis phenotype (Raymond et al., 2004) Rac1^J11, Rac2^Δ/Rac2[+] has adult mushroom body phenotype (Ng et al., 2002) Rac1^J11, Rac2^Δ/Rac2[+] has serotonergic neuron phenotype (Rodriguez Moncalvo and Campos, 2005) Rac1^J11, Rac2^Δ has adult mushroom body \| somatic clone phenotype (Ng et al., 2002, Ng et al., 2002) Rac1^J11, Rac2^Δ has adult mushroom body phenotype (Ng et al., 2002) Rac1^J11, Rac2^Δ has dorsal closure embryo phenotype (Denholm et al., 2005, Denholm et al., 2005) Rac1^J11, Rac2^Δ has dorsal group branch precursor phenotype (Chihara et al., 2003, Chihara et al., 2003) Rac1^J11, Rac2^Δ has dorsal trunk primordium phenotype (Chihara et al., 2003, Chihara et al., 2003) Rac1^J11, Rac2^Δ has embryonic anterior Malpighian tubule phenotype (Puetz et al., 2005, Puetz et al., 2005) Rac1^J11, Rac2^Δ has embryonic epidermis phenotype (Chihara et al., 2003, Chihara et al., 2003) Rac1^J11, Rac2^Δ has embryonic Malpighian tubule phenotype (Puetz et al., 2005, Puetz et al., 2005) Rac1^J11, Rac2^Δ has embryonic myoblast phenotype (Haralalka et al., 2011) Rac1^J11, Rac2^Δ has ganglionic branch primordium phenotype (Lundstrom et al., 2004, Lundstrom et al., 2004) Rac1^J11, Rac2^Δ has Malpighian tubule main body primordium phenotype (Puetz et al., 2005, Puetz et al., 2005) Rac1^J11, Rac2^Δ has photoreceptor cell & axon phenotype (Hakeda-Suzuki et al., 2002, Hakeda-Suzuki et al., 2002) Rac1^J11, Rac2^Δ has posterior spiracle primordium phenotype (Denholm et al., 2005, Denholm et al., 2005) Rac1^J11, Rac2^Δ has serotonergic neuron phenotype (Rodriguez Moncalvo and Campos, 2005, Rodriguez Moncalvo and Campos, 2005) Rac1^J11, Rac2^Δ has tracheal branch primordium phenotype (Chihara et al., 2003, Chihara et al., 2003) Rac1^J11, sli² has longitudinal connective phenotype (Fan et al., 2003, Fan et al., 2003) Rac1^J11/Rac1[+], Rac2^Δ/Rac2[+], prtp^Δ1 has embryonic hemocyte phenotype (Kuraishi et al., 2009) Rac1^J11/Rac1[+], Rac2^Δ has serotonergic neuron phenotype (Rodriguez Moncalvo and Campos, 2005)
Additional Comments
Genetic Interactions
Statement Reference Myoblast fusion is defective in Rac1[J11] Rac2[Δ] double mutant embryos, although some fusion does occur. Binucleate muscle precursors that have undergone a single fusion event between a founder cell and a fusion competent myoblast are seen in the mutant embryos as follows: DA1 (64.2% of segments), DO1 (68.3% of segments), LO1 (26.3% of segments), VT1 (66.7%) of segments. (Haralalka et al., 2011) A mild, though not significant enhancement of the ommatidium-phenotype resulting from the co-expression of Arf51F[GD13822] with Dcr-2[Scer\UAS.cDa] under the control of Scer\GAL4[GMR.PF] is observed in a Rac1[J11], Rac2[Δ], Mtl[Δ] heterozygous background. (Johnson et al., 2011) Expression of psidin[Scer\UAS.cKa] under the control of Scer\GAL4[slbo.2.6] in a Rac1[J11] Rac2[Δ] double heterozygous background has little or no effect on border cell migration. (Kim et al., 2011) No rhabdomeric defect is seen at the base of the rhabdomere at eclosion in flies in which the eyes are triply mutant for Rac1[J11] Rac2[Δ] Mtl[Δ], although there is an axon guidance defect in the optic lobe. (Pinal and Pichaud, 2011) The Rac1[J11]/+ heterozygous mutation causes a mild suppression of the GluRIIA[SP16] NMJ synaptic homeostatic response. The Exn[EY01953]/+ heterozygous mutation does not enhance the Rac1[J11]/+, GluRIIA[SP16] double mutant NMJ synaptic homeostasis phenotype. (Frank et al., 2009) Larval hemocytes prepared from flies simultaneously heterozygous for drpr[Δ5], Rac1[J11] and Rac2[Δ] show a significant reduction in the level of phagocytosis of S. aureus. (Hashimoto et al., 2009) Heterozygosity for Rac1[J11] enhances the phenotype in the alpha lobes of the mushroom bodies that is seen in PsGEF[Δ21] animals; a pair of alpha/beta lobes is missing in 90% of the double mutants. (Higuchi et al., 2009) prtp[Δ1]/+ ; Rac1[J11] Rac2[Δ]/+ triple heterozygotes show a reduced level of phagocytosis in embryonic hemocytes (neither prtp[Δ1]/+ single heterozygotes nor Rac1[J11] Rac2[Δ]/+ double heterozygotes show a reduction in phagocytosis compared to wild type). (Kuraishi et al., 2009) The rough eye phenotype resulting from Scer\GAL4[GMR.PU]-mediated expression of Ced-12[Scer\UAS.cGa] and mbc[Scer\UAS.cBa] is suppressed by heterozygosity for Rac1[J11], Rac2[Δ]. (Geisbrecht et al., 2008) A Rac1[J11] Rac2[Δ] Mtl[Δ] heterozygous background enhances the patterning defects found in Scer\GAL4[GMR.PF]>cindr[dsRNA.PC.PD.Scer\UAS] mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants. (Johnson et al., 2008) The Scer\GAL4[elav-C155]/DAAM[C.Scer\UAS.P\T] gain-of-function phenotype (i.e the appearance of thicker commissures and nerve roots) is not affected by Rac1 gene dose (i.e. a Rac1[J11]/+ or Rac1[J11]/Rac1[J10] background), a Rac1[J11], Rac2[Δ] background or a Rac1[J10], Rac2[Δ], Mtl[Δ] background. A Rac1[J11] background enhances the zygotic DAAM[Ex68] CNS phenotype, with the severity of the phenotype correlating with the number of Rac1 copies removed. A DAAM[Ex68] heterozygous background strongly enhances the axonal growth defects exhibited by Rac1[J11] mutants. A Rac1[J11]/+; Rac2[Δ]/+ background enhances both the zygotic DAAM[Ex68] and DAAM[Ex68]; Rac1[J11]/+ CNS phenotypes. A Rac1[J11]; Rac2[Δ], Mtl[Δ] heterozygous background enhances the zygotic DAAM[Ex68], DAAM[Ex68]; Rac1[J11]/+ and DAAM[Ex68]; Rac1[J11]/+; Rac2[Δ]/+CNS phenotypes. (Matusek et al., 2008) The increased bouton number at the neuromuscular junction that is seen in Vps35[EY14200]/Df(2R)ED3952 larvae is suppressed by Rac1[J11]/+. (Korolchuk et al., 2007) Rac1[J11], Rac2[Δ], Mtl[Δ] triple mutant clones in the eye result in a low frequency (approximately 5%) of planar polarity defects (such as achiral or misrotated ommatidia). Cdc42[5], Rac1[J11], Rac2[Δ], Mtl[Δ] quadruple mutant clones in the eye result in a higher frequency of (14.2%) of planar polarity defects. (Muñoz-Descalzo et al., 2007) Rac1[J11] Rac2[Δ] Mtl[Δ] triple mutant myoblasts show enlarged foci, as well as increased numbers of actin foci. Enlarged foci are seen in these mutants from the earliest stages of fusion and foci persist after fusion would be complete in wild-type. (Richardson et al., 2007) The salivary gland defects seen in Rac1[J11] embryos are enhanced in Rac1[J11] Rac2[Δ] mutants; more cells remain at the ventral surface of the embryo and the gland fails to migrate posteriorly. In addition, the lumen of the salivary gland is disrupted, with breaks in the lumen being seen during posterior migration of the gland, and cyst-like lumena being seen in the mature gland. shi[2] Rac1[J11] Rac2[Δ] Mtl[Δ] heterozygous embryos form normal salivary glands at the permissive temperature of 25[o]C. However, at the restrictive temperature of 30[o]C, posterior migration of the salivary gland is disrupted. (Pirraglia et al., 2006) The Rac1^J11 mutation fails to modify the cv-c^M62 phenotype in the Malpighian tubules. Dorsal closure defects occur in 82% of Rac1^J11, Rac2^Δ double mutant embryos. If these mutants also carry the cv-c^M62 mutation , 37 % of these embryos are rescued. In cv-c^M62, Rac1^J11, Rac2^Δ triple mutants, the posterior spiracle phenotype is enhanced compared to cv-c^M62 mutants. Posterior spiracle phenotypes are seen in Rac1^J11, Rac2^Δ embryos, but with low penetrance. (Denholm et al., 2005) A Rac1^J11/+ background enhances the splitting wing hair phenotype of flies that express trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ap-md544. (He et al., 2005) In Rac1^J11; Rac2^Δ double homozygous embryos, rearrangement of tubule cells to produce elongated, 2 cell wide tubules is partially disrupted and tubule migration is defective. The anterior tubules follow an erratic path, doubling back on themselves to form loops and knots. (Puetz et al., 2005) The penetrance of the LNv ectopic branch phenotype is increased to 100% and the posterior tract defasciculation phenotype is increased to 93% when Fmr1^Δ113M/+ flies are also heterozygous for Rac1^J11. Likewise, the LNv ectopic branch phenotype is increased to 100% and the posterior tract defasciculation phenotype is increased to 93% when Rac1^J11/+ flies are also heterozygous for Fmr1^Δ113M. (Reeve et al., 2005) Both Rac1^J11, Rac2^Δ/Rac2^Δ mutants and Rac2^Δ, Rac1^J11/Rac1^J11 mutants show a reduction of 5-HT arborization in the larval brain. (Rodriguez Moncalvo and Campos, 2005) Developmental dispersal of hemocytes is abnormal in embryos triply mutant for Rac1^J11, Rac2^Δ and Mtl^Δ. One hour after laser-induced wounding, approximately half the number of hemocytes are recruited to the wound in embryos triply mutant for Rac1^J11, Rac2^Δ and Mtl^Δ compared to wild type embryos. Hemocytes that are recruited in the triple mutant embryos have significantly reduced lamellar protrusions. (Stramer et al., 2005) Germ line clones of the Rac1^J11 Rac2^Δ Mtl^Δ triple mutant fail to produce embryos. Zygotic Rac1^J11 Rac2^Δ Mtl^Δ triple mutants, that have wild-type maternal contribution of Rac1, survive beyond early dorsal closure, but still show 100% embryonic lethality. These embryos achieve dorsal closure, but show puckering along the dorsal side. The dorsal hole becomes a long slit-like shape as it closes in the triple mutants, while the hole has an oval shape in wild-type embryos. Although amnioserosa cells are significantly larger in the mutants than in wild type, these cells are able to contract at a similar rate to wild type. The leading edge of zygotic Rac1^J11 Rac2^Δ Mtl^Δ triple mutants is disorganized and, unlike in wild-type embryos, is not taut. Many of the triple mutant leading edge cells are polygonal in shape, instead of being dorsally-ventrally elongated, like in wild type. Some of the cells in the mutant edge assemble the actin cable and actin projections, while other cells fail to do so. Leaky expression of hep^CA.Scer\UAS, with no GAL4 driver, partially rescues the lethality and dorsal puckering phenotype of Rac1^J11 Rac2^Δ Mtl^Δ triple mutants. (Woolner et al., 2005) Rac1^J11/+ has very little effect on the mutant wing phenotype caused by expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E (the % of wings with normal morphology at 18^oC is 23% compared to 9% for control flies expressing LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E in an otherwise wild-type background). The frequency of the malformed leg phenotype seen in Sb^63b/+ heterozygotes (8%) is not increased if the flies are also heterozygous for Rac1^J11/+ (3%). The frequency of the malformed leg phenotype seen in Sb⁷⁰/+ heterozygotes (7%) is not increased if the flies are also heterozygous for Rac1^J11/+ (2%). The frequency of the malformed leg phenotype seen in br¹/Y males (1%) is not increased if the flies are also heterozygous for Rac1^J11/+ (0%). (Chen et al., 2004) Rac1^J11, Rac2^Δ mutant embryos exhibit arrested ganglionic branches (GBs), and GBs turning prematurely away from the midline. robo⁴ partially suppresses the ganglionic branch phenotype seen in Rac1^J11, Rac2^Δ embryos. (Lundstrom et al., 2004) 74% of Rac1[J11] cdi[R47]/Rac1[J11] males are sterile in individual crosses with females. The males have an abnormal accumulation of dense material in the distal part of the testis. (Raymond et al., 2004) Double mutant clones of Rac1^J11 and Rac2^Δ do not affect F-actin enrichment in rhabdomeres. (Zelhof and Hardy, 2004) In a subset of the epidermis of stage 15 Rac1^J11, Rac2^Δ double homozygous embryos the distinction between apical and basolateral domains is compromised. At stage 16 the columnar epithelial cells are shorter than wild-type and parts of the epidermis become multilayered. Reducing the maternal and zygotic gene dosage of Rac1^J11 and Rac2^Δ together produces embryos exhibiting tracheal defects of increasing severity: The mildest phenotype is a misrouting of the dorsal branches toward the anteroposterior direction, whereas more severely effected embryos also exhibit truncations of the dorsal trunk. Order of severity: maternal(M) +/+ zygotic(Z) +/- =WT. M -/+ Z +/+ < M -/+, Z -/+ < M +/-, Z -/-. The proportion of M -/+, Z -/+ embryos with severely effected tracheal systems is enhanceable by heterozygosity for bnl⁰⁰⁸⁵⁷, btl^ΔOh10, or stumps^09904b. (Chihara et al., 2003) In a subset of the epidermal cells of stage 15 Rac1^J11, Rac2^Δ double homozygous embryos, the distinction between apical and basolateral domains is compromised. At stage 16 the columnar epithelial cells are shorter than wild-type and parts of the epidermis become multilayered. Reducing the maternal and zygotic gene dosage of Rac1^J11 and Rac2^Δ together produces embryos exhibiting tracheal defects of increasing severity: the mildest phenotype is a mis-routing of the dorsal branches toward the anteroposterior direction, whereas more severely affected embryos also exhibit truncations of the dorsal trunk. Order of severity: maternal(M) +/+ zygotic(Z) +/- =WT. M -/+ Z +/+ < M -/+, Z -/+ < M +/-, Z -/-. The proportion of M -/+, Z -/+ embryos with severely affected tracheal systems is enhanceable by heterozygosity for bnl⁰⁰⁸⁵⁷, btl^ΔOh10, or stumps^09904b. (Chihara et al., 2003) The combination of heterozygous sli² and Rac1^J11 leads to an enhancement of the longitudinal axon ectopic midline crossing defects. An average of 4.8 defects are seen per animal, and an average of 44% of segments have defects. (Fan et al., 2003) Rac1^J11 Rac2^Δ Mtl^Δ triple mutant clones in the wing and eye do not show planar cell polarity defects. Mosaic flies in which the eye is doubly mutant for Rac1^J11 and Rac2^Δ show mild defects in the projection pattern of photoreceptor cell axons. Mosaic flies in which the eye is triply mutant for Mtl^Δ, Rac1^J11 and Rac2^Δ show severe defects in the projection pattern of photoreceptor cell axons, showing a medulla bypass phenotype. Specification of photoreceptor cell fate appears to be normal. (Hakeda-Suzuki et al., 2002) 55% of Rac1^J11 Rac2^Δ double mutant neuroblast clones show defective guidance. 55% of Rac1^J11 Rac2^Δ Mtl^Δ single-cell γ neuron clones in the mushroom body show axon-stalling defects, mostly at the peduncle. There is a significant reduction in total dendritic length and number of dendritic segments per neuron compared to wild type. Mushroom body axon growth defects in single-cell Rac1^J11 Rac2^Δ Mtl^Δ γ neuron clones are largely rescued by expression of Rac1^{Scer\UAS.T:Hsap\MYC} or Rac1^{Y40C.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^OK107. Mushroom body axon growth defects in single-cell Rac1^J11 Rac2^Δ Mtl^Δ γ neuron clones are not rescued by expression of Rac1^{F37A.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^OK107. 81% of axons in Rac1^J11 Rac2^Δ mushroom body clones show mutant phenotypes, predominantly guidance (55%) and branching (24%) defects. Expression of Rac1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^OK107 markedly rescues these defects. Expression of Rac1^{Y40C.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^OK107 does not reduce the total percentage of axonal defects in Rac1^J11 Rac2^Δ mushroom body clones, but results in a marked shift in the distribution of defects, with most showing branching (45%) rather than guidance (31%) defects. Analysis of Rac1^J11 Rac2^Δ mushroom body clones indicates cell non-autonomous effects in axon guidance and branching caused by defective "Rac" activity. (Ng et al., 2002) The phenotype caused by expression of msn^EP549 under the control of Scer\GAL4^GMR.PF is not modified by the addition of the triple mutant combination Rac1^J11 Rac2^Δ Mtl^Δ. (Ruan et al., 2002)
Xenogenetic Interactions
Statement Reference Rac1[J11]/+ does not suppress the wing defects caused by expression of HIV-1\Vpu[Scer\UAS.cLa] under the control of Scer\GAL4[dpp.blk1]. The presence of Rac1[J11]/+ and Rac2[Δ]/+ in double heterozygous combination does not suppress the wing defects caused by expression of HIV-1\Vpu[Scer\UAS.cLa] under the control of Scer\GAL4[dpp.blk1]. (Marchal et al., 2012)
Complementation & Rescue Data
Rescued by	Rac1^J11/Df(3L)Rac1 is rescued by Rac1^+t3.6 (Ng et al., 2002)
Comments	Expression of Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[sns.PK] strongly rescues the myoblast fusion defects of Rac1[J11] Rac2[Δ] double mutant embryos, resulting in a near normal somatic muscle pattern. In contrast, expression of Rac1[Scer\UAS.cLa] under the control of Scer\GAL4[kirre-rP298] rescues the myoblast fusion defects of Rac1[J11] Rac2[Δ] double mutant embryos much less efficiently. (Haralalka et al., 2011)
Stocks ( 3 )
Bloomington	6674 y¹ w^; Rac1^J11 P{FRT(w^hs)}2A/TM6B, Tb⁺ 6677 y¹ w^; Rac1^J11 Rac2^Δ P{FRT(w^hs)}2A/TM6B, Tb⁺ 6678 y¹ w^*; Rac1^J11 Rac2^Δ P{FRT(w^hs)}2A Mtl^Δ/TM6B, Tb¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
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Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Drac1^J11 (He et al., 2005) rac1^J11 (Fan et al., 2003, Lundstrom et al., 2004, Chihara et al., 2003, Kim et al., 2011, Pinal and Pichaud, 2011, Haralalka et al., 2011, Higuchi et al., 2009, Johnson et al., 2008) Rac1^J11 (Stramer et al., 2005, Puetz et al., 2005, Johnson et al., 2011, Pirraglia et al., 2006, Avet-Rochex et al., 2007, Korolchuk et al., 2007, Sampson et al., 2012, Rothenfluh et al., 2006, Frank et al., 2009, Geisbrecht et al., 2008, Kuraishi et al., 2009, Paladi and Tepass, 2004, Djiane and Mlodzik, 2010, Matusek et al., 2008, Richardson et al., 2007, Muñoz-Descalzo et al., 2007, Hashimoto et al., 2009) Rac1^j11 (Srahna et al., 2006, Neisch et al., 2010, Raymond et al., 2004, Marchal et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 45 )

Generate a list of	All references relating to this allele ( 45 )

List References by type	Research paper ( 45 )
Recent research papers ( 6 )
	Marchal et al., 2012, PLoS ONE 7(3): e34310 The HIV-1 Vpu Protein Induces Apoptosis in Drosophila via Activation of JNK Signaling. [FBrf0218002] Sampson et al., 2012, Dev. Comp. Immunol. 38(1): 160--168 The RhoGEF Zizimin-related acts in the Drosophila cellular immune response via the Rho GTPases Rac2 and Cdc42. [FBrf0219019] Haralalka et al., 2011, Development 138(8): 1551--1562 Asymmetric Mbc, active Rac1 and F-actin foci in the fusion-competent myoblasts during myoblast fusion in Drosophila. [FBrf0213340] Johnson et al., 2011, Mol. Biol. Cell 22(23): 4513--4526 Role for a Cindr-Arf6 axis in patterning emerging epithelia. [FBrf0216708] Kim et al., 2011, Genes Dev. 25(7): 730--741 Psidin, a conserved protein that regulates protrusion dynamics and cell migration. [FBrf0213388] Pinal and Pichaud, 2011, J. Cell Sci. 124(9): 1564--1570 Dynamin- and Rab5-dependent endocytosis is required to prevent Drosophila photoreceptor degeneration. [FBrf0213465] Show all research papers ...

Allele Dmel\Rac1J11

Allele Dmel\Rac1^J11