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General Information
Symbol
Dmel\Ork1Δ-C.UAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0135891
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dORK, UAS-orkΔc
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a truncated form of Ork1 (lacking amino acids 299 to 1001) that is tagged between amino acid residues 288 and 289 with EGFP. Encodes a conducting form of Ork1.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Hsap\RELAAD.R92A10.T:Zzzz\Zip+ Scer\GAL4DBD.R17D06.T:Zzzz\Zip--mediated expression of Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP does not trigger basal rhythmic depolarization in electroretinograms.

Single v'ada class IV dendritic arborization neuron cells expressing Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4ppk.PU show a ventral shift in the position of their presynaptic terminals compared to controls. The topography of the presynaptic terminals of ddaC or vdaB class IV da neurons is unaffected if they express Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4ppk.PU as single cell clones.

Flies expressing Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4104Y show reduced night-time sleep compared to controls.

Expression of Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP under the control of any one of Scer\GAL4NP423, Scer\GAL4NP867 or Scer\GAL4NP394 leads to positive larval phototaxis, whereas controls show negative phototaxis.

Whole-cell current-clamp recordings from the somata in isolated, intact brain preparations reveals that expression of Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Mz19 efficiently silences projection neurons. The silencing of the Scer\GAL4Mz19-positive projection neurons induces increased active zone density. The microglomeruli formed by the clawed dendritic endings of mushroom body Kenyon cells associated with silenced projection neurons are more numerous compared with controls.

Electrical silencing of mdIV neurons through expression of Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4ppk.1.9 results in larvae that fail to display intermediate surfacing transition (IST) behavior (emerging from the food transiently to to roam the food surface, prior to complete food exit) seen in wild type. Eventual food exit is slightly delayed compared to controls. the resulting adult flies are smaller than their wild type counterparts.

Expression of Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP in the whole cardiac tube during larval stages, driven by Scer\GAL4NP1029, results in the arrest of heart beating.

Expression of Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4ato.3.6 does not affect dorsal cluster neuron axon extension across the optic chiasm in adult flies.

Most embryos expressing Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4elav-C155 fail to hatch and the rest show very sluggish movement as first instar larvae and fail to reach the second instar. Expression of Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4P2.4.Pdf does not alter the number of adult lateral pacemaker neurons, and they show their normal dorso-medial projection into the central brain. 72% of flies expressing P{UAS-Ork1.Δ-C}1 under the control of Scer\GAL4P2.4.Pdf are arrhythmic with respect to free-running locomotor activity. In LD conditions, flies expressing Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4P2.4.Pdf begin to increase their activity about 2 hours before lights-off (as do wild-type flies).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Neuronal silencing of mdIV neurons through expression of Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP suppresses the reduction in adult body size seen when Bhal\NaChBacScer\UAS.T:Avic\GFP-EGFP is expressed under the control of Scer\GAL4ppk.1.9.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP
Ork1Δ-C.Scer\UAS.T:Avic\GFP-F64L.S65T
Ork1Δ-C.UAS.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (22)