Whole-cell current-clamp recordings from the somata in isolated, intact brain preparations reveals that expression of Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Mz19 efficiently silences projection neurons. The silencing of the Scer\GAL4Mz19-positive projection neurons induces increased active zone density. The microglomeruli formed by the clawed dendritic endings of mushroom body Kenyon cells associated with silenced projection neurons are more numerous compared with controls.
Electrical silencing of mdIV neurons through expression of Ork1Δ-C.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4ppk.1.9 results in larvae that fail to display intermediate surfacing transition (IST) behavior (emerging from the food transiently to to roam the food surface, prior to complete food exit) seen in wild type. Eventual food exit is slightly delayed compared to controls. the resulting adult flies are smaller than their wild type counterparts.