FB2025_01 , released February 20, 2025
Allele: Dmel\Nop60BRNAi.Sym.UAS
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General Information
Symbol
Dmel\Nop60BRNAi.Sym.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0135895
Feature type
allele
Associated gene
Dmel\Nop60B
Associated Insertion(s)
Carried in Construct
P{Sym-UAS-Nop60B}
Key Links
Transgenic product class
RNAi_reagent
(Giordano et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Giordano et al., 2002)
Mutagen
in vitro construct
(Giordano et al., 2002)
Progenitor genotype
Carried in construct
P{Sym-UAS-Nop60B}
Cytology
Description

Sense-antisense transcription (driven by two convergent arrays of UAS regulatory sequences) of a 1.7kb fragment corresponding to nucleotides +619 to +2327 of Nop60B (coordinates according to AF097634) results in double stranded RNA (dsRNA) expression.

(Giordano et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Nop60B
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Simultaneous expression of Nop60BSym.Scer\UAS and Nop60Ba.Sym.Scer\UAS (both carried in P{Sym-UAS-Nop60B}) under the control of Scer\GAL4Act5C.PI results in developmental delay and reduced female fertility. 90% of the flies have hatched abdominal cuticle and a reduction in the number, length and thickness of bristles. Simultaneous expression of Nop60BSym.Scer\UAS and Nop60Ba.Sym.Scer\UAS (both carried in P{Sym-UAS-Nop60B}) under the control of Scer\GAL4pnr-MD237 results in a reduction in the number, length and thickness of bristles only along the dorsal midline (the domain of Scer\GAL4pnr-MD237 expression).

      Expression of Nop60B[dsRNA.Sym.ScerUAS] under the control of ScerGAL4[Act5C.PI] results in developmental delay and reduced female fertility. 90% of the flies have hatched abdominal cuticle and a reduction in the number, length and thickness of bristles. Expression of Nop60B[dsRNA.Sym.ScerUAS] under the control of ScerGAL4[pnr-MD237] results in a reduction in the number, length and thickness of bristles only along the dorsal midline (the domain of ScerGAL4[pnr-MD237] expression).

      (Giordano et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Nop60BRNAi.Sym.UAS
      Nop60BSym.Scer\UAS
      Nop60BdsRNA.Sym.Scer\UAS
      Nop60BdsRNA.Sym.UAS
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0135894
      References (2)