FlyBase Allele Report: Dmel\Eph[DN.UAS]

General Information

Symbol

Dmel\Eph^DN.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0135987

Feature type

allele

Associated gene

Dmel\Eph

Associated Insertion(s)

Carried in Construct

P{UAS-eph.DN}

Key Links

Transgenic product class

inframe_deletion

(Dearborn et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Dearborn et al., 2002)

Mutagen

in vitro construct

(Dearborn et al., 2002)

Progenitor genotype

Eph^UAS.cDa

(Dearborn et al., 2002)

Carried in construct

P{UAS-eph.DN}

Cytology

Description

Lacks residues 675-1035 i.e. consists of normal extracellular and transmembrane domains but lacks most of the intracellular domain including the kinase, SAM and PDZ-binding domains.

(Dearborn et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Eph

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ap-md544

(Dearborn et al., 2002)

abnormal neuroanatomy, with Scer\GAL4^ey.PH

(Dearborn et al., 2002)

abnormal neuroanatomy, with Scer\GAL4^αTub84B.PP

(Dearborn et al., 2002)

visible, with Scer\GAL4^ap-md544

(Dearborn et al., 2002)

Phenotype Manifest In

cell body rind of adult lobula plate, with Scer\GAL4^ey.PH

(Dearborn et al., 2002)

cell body rind of adult medulla, with Scer\GAL4^ap-md544

(Dearborn et al., 2002)

cell body rind of adult medulla, with Scer\GAL4^ey.PH

(Dearborn et al., 2002)

cell body rind of adult medulla | somatic clone, with Scer\GAL4^αTub84B.PP

(Dearborn et al., 2002)

embryonic/larval optic lobe, with Scer\GAL4^ey.PH

(Dearborn et al., 2002)

medulla anlage, with Scer\GAL4^ey.PH

(Dearborn et al., 2002)

photoreceptor cell & axon, with Scer\GAL4^ey.PH

(Dearborn et al., 2002)

photoreceptor cell R7 & axon | somatic clone, with Scer\GAL4^αTub84B.PP

(Dearborn et al., 2002)

photoreceptor cell R8 & axon | somatic clone, with Scer\GAL4^αTub84B.PP

(Dearborn et al., 2002)

Detailed Description

Statement

Reference

Expression of Eph^DN.Scer\UAS with Scer\GAL4^elav-C155 or Scer\GAL4^C57 has no significant effect on spontaneous miniature release event (mepsp) amplitudes or quantal content at the neuromuscular junction (NMJ), as compared to wild-type.

(Frank et al., 2009)

When expression is driven by Scer\GAL4^ey.PH severe defects are produced in the midline projections of photoreceptor and medulla cortical axon projections. Separation into dorsal and ventral fascicles at the midline of the lamina fails. The large fascicle often projects to ectopic locations. At the midline of the medulla the optic ganglion is disrupted - medulla neuropil is primarily absent and the area devoid of neurons fills with glia that would normally border the neuropil. When expression is driven by Scer\GAL4^ap-md544, a visible phenotype results. Axons of midline cortical cells project abnormally, though those in dorsal and ventral positions project normally. When expression is driven in somatic cell clones in the developing eye, by Scer\GAL4^αTub84B.PP, R7 and R8 axons are abnormally fasciculated when the clone is large. When the clone includes the cortex the defects map largely to the midline. Cortical cell axon projection defects are enhanced at the border between clone and non-clone tissue. Projections dorsoventral to the clone are generally wild type.

(Dearborn et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^C57/Eph^DN.UAS is a non-enhancer of abnormal neurophysiology | third instar larval stage | recessive phenotype of GluRIIA^SP16

(Frank et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Eph^DN.UAS is a suppressor of abnormal neurophysiology | recessive | third instar larval stage phenotype of GluRIIA^SP16

(Frank et al., 2009)

Eph^DN.UAS, Scer\GAL4^unspecified is a suppressor of visible phenotype of Ephrin^unspecified, Scer\GAL4^unspecified

(Dearborn et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^C57/Eph^DN.UAS is a non-suppressor of abnormal neurophysiology | recessive | third instar larval stage phenotype of GluRIIA^SP16

(Frank et al., 2009)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Scer\GAL4^C57/Eph^DN.UAS is a non-enhancer of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

Suppressor of

Statement

Reference

Eph^DN.UAS, Scer\GAL4^hs.2sev is a suppressor of eye phenotype of Ephrin^UAS.cDa, Scer\GAL4^hs.2sev

(Dearborn et al., 2012)

Scer\GAL4^elav-C155/Eph^DN.UAS is a suppressor of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

Eph^DN.UAS, Scer\GAL4^unspecified is a suppressor of eye phenotype of Ephrin^unspecified, Scer\GAL4^unspecified

(Dearborn et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^C57/Eph^DN.UAS is a non-suppressor of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype of Scer\GAL4^hs.2sev, Ephrin^Scer\UAS.cDa flies is suppressed by co-expression of Eph^DN.Scer\UAS.

(Dearborn et al., 2012)

Expression of Eph^DN.Scer\UAS with Scer\GAL4^C57 does not significantly change the GluRIIA^SP16 phenotype involving spontaneous miniature release event (mepsp) amplitudes or quantal content at the neuromuscular junction (NMJ).

Expression of Eph^DN.Scer\UAS with Scer\GAL4^elav-C155 does not significantly change the GluRIIA^SP16 phenotype involving spontaneous miniature release event (mepsp) amplitudes, while it does partially suppress the GluRIIA^SP16 quantal content phenotype at the neuromuscular junction (NMJ).

(Frank et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Eph^DN.UAS is rescued by Eph^UAS.cDa/Scer\GAL4^ap-md544

(Dearborn et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Eph^DN.Scer\UAS

Eph^DN.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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