FlyBase Allele Report: Dmel\Atx2[EP3145]

General Information

Symbol

Dmel\Atx2^EP3145

Species

D. melanogaster

Name

FlyBase ID

FBal0136144

Feature type

allele

Associated gene

Dmel\Atx2

Associated Insertion(s)

P{EP}Atx2^EP3145

Carried in Construct

Also Known As

EP(3)3145

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Pena-Rangel et al., 2002, Satterfield et al., 2002)

Progenitor genotype

Associated Insertion(s)

P{EP}Atx2^EP3145

Cytology

Description

P{EP} insertion upstream of Atx2.

(Sun et al., 2006)

The orientation of the P{EP} element is the same as the direction of Atx2 transcription.

(Shulman and Feany, 2003)

P{EP} insertion in the 5' untranslated region, 667bp upstream of the putative start codon. The P{EP} insertion is oriented such that overexpression from the element can drive overexpression of Atx2.

(Satterfield et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:15,408,455..15,408,455 [+]

(Rumpf et al., 2014, Elden et al., 2010, Al-Ramahi et al., 2007, Sofola et al., 2007, Sun et al., 2006, Ghosh and Feany, 2004, Shulman and Feany, 2003, Pena-Rangel et al., 2002, Satterfield et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Shulman and Feany, 2003)

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82.UAS

(Ghosh and Feany, 2004, Shulman and Feany, 2003)

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Ghosh and Feany, 2004)

exacerbates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Ghosh and Feany, 2004)

exacerbates amyotrophic lateral sclerosis

modeled by Hsap\TARDBP^UAS.cEa

(Elden et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Satterfield et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy phenotype of Hsap\TARDBP^UAS.cEa, Scer\GAL4^GMR.PF

(Elden et al., 2010)

Atx2^EP3145, Scer\GAL4^elav-C155 is an enhancer of short lived phenotype of Hsap\TARDBP^UAS.cEa, Scer\GAL4^elav-C155

(Elden et al., 2010)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, lap^EP3060

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, kay^EP3084

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of abnormal eye color phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Scer\GAL4^pnr-MD237/Atx2^EP3145 is an enhancer of visible phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

Other

Statement

Reference

Atx2^EP3145, Scer\GAL4^GMR.PF, XNP^EP635 has visible phenotype

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF, jigr1^EP3354 has visible phenotype

(Sun et al., 2006)

Atx2^EP3145, BicDR^EP3705, Scer\GAL4^GMR.PF has visible phenotype

(Sun et al., 2006)

Atx2^EP3145, D1^EP473, Scer\GAL4^GMR.PF has visible phenotype

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF, jing^UAS.cSa has visible phenotype

(Sun et al., 2006)

Atx2^EP3145, EP893^EP893, Scer\GAL4^GMR.PF has visible phenotype

(Sun et al., 2006)

Atx2^EP3145, Hsap\ATXN1^82.UAS, Scer\GAL4^ChAT.7.4 has lethal phenotype

(Ghosh and Feany, 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\TARDBP^UAS.cEa, Scer\GAL4^GMR.PF

(Elden et al., 2010)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, kay^EP3084

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, kay^EP3084

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of pigment cell phenotype of Scer\GAL4^GMR.PF, kay^EP3084

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, lap^EP3060

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Ghosh and Feany, 2004)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Ghosh and Feany, 2004)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Ghosh and Feany, 2004)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Atx2^EP3145, Scer\GAL4^GMR.PF is an enhancer of pigment cell phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

NOT Enhancer of

Statement

Reference

Atx2^EP3145, Scer\GAL4^30Y is a non-enhancer of adult brain phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^30Y

(Ghosh and Feany, 2004)

NOT Suppressor of

Statement

Reference

Atx2^EP3145, Scer\GAL4^30Y is a non-suppressor of adult brain phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^30Y

(Ghosh and Feany, 2004)

Other

Statement

Reference

Atx2^EP3145, Scer\GAL4^GMR.PF, XNP^EP635 has eye phenotype

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF, jigr1^EP3354 has pigment cell phenotype

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF, jigr1^EP3354 has eye phenotype

(Sun et al., 2006)

Atx2^EP3145, BicDR^EP3705, Scer\GAL4^GMR.PF has eye phenotype

(Sun et al., 2006)

Atx2^EP3145, BicDR^EP3705, Scer\GAL4^GMR.PF has ommatidium phenotype

(Sun et al., 2006)

Atx2^EP3145, D1^EP473, Scer\GAL4^GMR.PF has eye phenotype

(Sun et al., 2006)

Atx2^EP3145, D1^EP473, Scer\GAL4^GMR.PF has ommatidium phenotype

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF, lap^EP3060 has pigment cell phenotype

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF, jing^UAS.cSa has eye phenotype

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF, jing^UAS.cSa has ommatidium phenotype

(Sun et al., 2006)

Atx2^EP3145, EP893^EP893, Scer\GAL4^GMR.PF has eye phenotype

(Sun et al., 2006)

Atx2^EP3145, EP893^EP893, Scer\GAL4^GMR.PF has ommatidium phenotype

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^GMR.PF, XNP^EP635 has pigment cell phenotype

(Sun et al., 2006)

Atx2^EP3145, Scer\GAL4^pnr-MD237, pnr^MD237 has scutellum phenotype

(Pena-Rangel et al., 2002)

Atx2^EP3145, Scer\GAL4^pnr-MD237, pnr^MD237/pnr[+] has scutellum phenotype

(Pena-Rangel et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by Atx2^EP3145. Atx2^EP3145 enhances the eye phenotype caused by expression of Hsap\ATX1^82.Scer\UAS under the control of Scer\GAL4^GMR.PF; loss of eye pigmentation is enhanced and necrotic black spots are induced.

(Shulman and Feany, 2003)

pnr^MD237/+ adults expressing Atx2^EP3145 under the control of Scer\GAL4^pnr-MD237 have a deformed scutellum.

(Pena-Rangel et al., 2002)

Xenogenetic Interactions

Statement

Reference

Overexpression of Atx2^EP3145 via Scer\GAL4^GMR.PF enhances the toxicity of Hsap\TARDBP^Scer\UAS.cEa in a markedly dose-dependent manner resulting in more severe retinal degeneration.

Overexpression of Atx2^EP3145 via Scer\GAL4^elav-C155 enhances the toxicity of Hsap\TARDBP^Scer\UAS.cEa in a markedly dose-dependent manner resulting in a further reduction of lifespan.

(Elden et al., 2010)

Complementation and Rescue Data

Complements

Atx2^EP3022

(Satterfield et al., 2002)

Atx2⁰⁶⁴⁹⁰

(Satterfield et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237.

(Pena-Rangel et al., 2002)

Separable from: a recessive lethal mutation unrelated to the P{EP}Atx2^EP3145 insertion.

(Satterfield et al., 2002)

Comments

Precise excision of the P{EP} element reverts the modifying effect of Atx2^EP3145 on the rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Shulman and Feany, 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Atx2^EP3145

(Sofola et al., 2007)

BEST:GH27029^EP3145

(Pena-Rangel et al., 2002)

EP(3)3145

(Lessing and Bonini, 2008)

EP(3)3145V

(Satterfield et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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