Allele Dmel\Syt1^Scer\UAS.cMa

General Information
Symbol	Dmel\Syt1^Scer\UAS.cMa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Mackler	FlyBase ID	FBal0137072
Feature type	allele	Associated gene	Dmel\Syt1

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Mackler and Reist, 2001, Mackler et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of wild-type syt. (Mackler and Reist, 2001)
Carried in construct	P{UAS-syt.M} (Mackler et al., 2002, Mackler et al., 2002, Mackler and Reist, 2001, Mace et al., 2009, Poskanzer et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	neurophysiology defective, with Scer\GAL4^elav.PLu, Syt1^AD4 (Mackler and Reist, 2001) uncoordinated, with Scer\GAL4^elav.PLu, Syt1^AD4 (Mackler and Reist, 2001)
Phenotype Manifest In

Detailed Description
	Statement Reference There is no difference in τ values in Syt1[AD4]/Syt1[N13]; Syt1[Scer\UAS.cMa] Scer\GAL4[elav.PLu] flies in response to a 50Hz, 10s stimulus. (Poskanzer et al., 2006) The Ca²⁺ dependence of neurotransmitter release in larvae expressing syt^{B-D3.4N.Scer\UAS} under the control of Scer\GAL4^elav.PLu in a syt^AD4 background is significantly shifted compared to the Ca²⁺ dependence of neurotransmitter release in larvae expressing syt^Scer\UAS.cMa under the control of Scer\GAL4^elav.PLu in a syt^AD4 background. (Mackler et al., 2002)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Syt1^Scer\UAS.cMa/Scer\GAL4^elav.PLu rescues Syt1^AD4/Syt1^N13 (Poskanzer et al., 2006)
Partially rescues	Syt1^Scer\UAS.cMa/Scer\GAL4^elav.PLu partially rescues Syt1^AD4 (Mackler and Reist, 2001)
Comments	Expression of syt^Scer\UAS.cMa under the control of Scer\GAL4^elav.PLu rescues the lethality of syt^AD4 flies; 85 +/- 6% of the expected number survive. The rescued adults are slightly uncoordinated. syt^AD4 homozygous larvae which are also expressing syt^Scer\UAS.cMa under the control of Scer\GAL4^elav.PLu have a mean excitatory junction potential (EJP) amplitude at the neuromuscular junction which is 67% of that of control larvae. The frequency of spontaneous miniature EJPs (mEJPs) in these larvae is slightly increased compared to controls (the difference is not statistically significant). The distribution of quantal sizes and the mean quantal size of the mEJPs is not different from controls. The mean excitatory junction potential (EJP) amplitude at the neuromuscular junction of syt^AD4 homozygous larvae which are also expressing syt^KQ.Scer\UAS under the control of Scer\GAL4^elav.PLu is significantly reduced compared to the mean EJP amplitude of syt^AD4 homozygous larvae which are also expressing syt^Scer\UAS.cMa under the control of Scer\GAL4^elav.PLu. The mean miniature EJP (mEJP) amplitude at the neuromuscular junction of syt^AD4 homozygous larvae which are also expressing syt^KQ.Scer\UAS under the control of Scer\GAL4^elav.PLu is not significantly different from the mean mEJP amplitude of syt^AD4 homozygous larvae which are also expressing syt^Scer\UAS.cMa under the control of Scer\GAL4^elav.PLu. The mean mEJP frequency at the neuromuscular junction of syt^AD4 homozygous larvae which are also expressing syt^KQ.Scer\UAS under the control of Scer\GAL4^elav.PLu is approximately twice that of syt^AD4 homozygous larvae which are also expressing syt^Scer\UAS.cMa under the control of Scer\GAL4^elav.PLu. (Mackler and Reist, 2001)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Syt1^Scer\UAS.cMa syt^Scer\UAS.cMa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Mackler (Mackler and Reist, 2001)
Secondary FlyBase IDs

References ( 5 )
Research paper	Mace et al., 2009, genesis 47(5): 337--345 Synaptotagmin I stabilizes synaptic vesicles via its C(2)A polylysine motif. [FBrf0215317] Poskanzer et al., 2006, Neuron 50(1): 49--62 Discrete residues in the c(2)b domain of synaptotagmin I independently specify endocytic rate and synaptic vesicle size. [FBrf0195323] Mackler et al., 2002, Nature 418(6895): 340--344 The C(2)B Ca(2+)-binding motif of synaptotagmin is required for synaptic transmission in vivo. [FBrf0151437] Mackler and Reist, 2001, J. Comp. Neurol. 436(1): 4--16 Mutations in the second C[2] domain of synaptotagmin disrupt synaptic transmission at Drosophila neuromuscular junctions. [FBrf0136999]
Supplementary material	Mackler et al., 2002, Nature 418(6895): The C(2)B Ca(2+)-binding motif of synaptotagmin is required for synaptic transmission in vivo. [FBrf0150845]

Allele Dmel\Syt1Scer\UAS.cMa

Allele Dmel\Syt1^Scer\UAS.cMa