|Feature type||allele||Associated gene||Dmel\Syt1|
|Mutagen||in vitro construct - amino acid replacement, in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Construct: Amino acid replacement: D416N. Amino acid replacement: D418N. Scer\UAS regulatory sequences drive expression of a mutated form of syt.
Construct: Amino acid replacement: D416N. Amino acid replacement: D418N.
|Carried in construct|
|Phenotype Manifest In|
Expression of Syt1[B-D3.4N.Scer\UAS] under the control of Scer\GAL4[elav.PLu] largely suppresses the asynchronous component of release seen in Syt1[N13]/Syt1[AD4] embryos when evoked release properties are recorded from muscle 6 following motor nerve stimulation in 4mM extracellular Ca[2+]. Only a small amount of residual synchronous release is seen in these embryos and the total charge transfer is dramatically reduced, similar to Syt1[N13]/Syt1[AD4] embryos. Syt1[N13]/Syt1[AD4] embryos expressing Syt1[B-D3.4N.Scer\UAS] under the control of Scer\GAL4[elav.PLu] show an enhanced rate of spontaneous release compared to controls. Embryos expressing Syt1[B-D3.4N.Scer\UAS] under the control of Scer\GAL4[elav.PLu] in a Syt1[N13]/Syt1[AD4] background show a similar level of hypertonic stimulated release as controls.
Following a 50 Hz, 10 s stimulus, the peak ΔF/F SpH change in Syt1[AD4]/Syt1[N13]; Syt1[B-D3.4N.Scer\UAS] Scer\GAL4[elav.PLu]flies is reduced compared to controls, which agrees with prior electrophysiological demonstration of a decrease in evoked release. A slowed endocytosis rate constant is found at Syt1[B-D3.4N.Scer\UAS] rescue synapses, with an average endocytic value that is more than 2-fold slower than controls. Increasing the Ca[2+] concentration speeds the rate of endocytosis, although not to control levels.
Larvae expressing sytB-D3.4N.Scer\UAS under the control of Scer\GAL4elav.PLu in a sytAD4 background show a reduced mean excitatory junction potential (EJP) amplitude at the neuromuscular junction compared to sytAD4 mutant larvae. The Ca2+ dependence of neurotransmitter release in larvae expressing sytB-D3.4N.Scer\UAS under the control of Scer\GAL4elav.PLu in a sytAD4 background is significantly shifted compared to the Ca2+ dependence of neurotransmitter release in larvae expressing sytScer\UAS.cMa under the control of Scer\GAL4elav.PLu in a sytAD4 background. Expression of sytB-D3.4N.Scer\UAS under the control of Scer\GAL4elav.PLu in an otherwise wild-type background reduces the mean EJP amplitude at the neuromuscular junction by 53% compared to wild type.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Syt1 <up>D416N, D418N</up>
|Secondary FlyBase IDs|
|References ( 4 )|