Allele Dmel\Syt1^{B-D3.4N.Scer\UAS}

General Information
Symbol	Dmel\Syt1^{B-D3.4N.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0137076
Feature type	allele	Associated gene	Dmel\Syt1

Allele class
Mutagen	in vitro construct - amino acid replacement, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Mackler et al., 2002, Mackler et al., 2002) in vitro construct - regulatory fusion (Mackler et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Amino acid replacement: D416N. Amino acid replacement: D418N. Scer\UAS regulatory sequences drive expression of a mutated form of syt. (Mackler et al., 2002) Construct: Amino acid replacement: D416N. Amino acid replacement: D418N. (Mackler et al., 2002)
Carried in construct	P{UAS-syt.B-D3,4N} (Mackler et al., 2002, Mackler et al., 2002, Poskanzer et al., 2006, Yoshihara et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	neurophysiology defective, with Scer\GAL4^elav.PLu (Mackler et al., 2002) neurophysiology defective, with Scer\GAL4^elav.PLu, Syt1^AD4 (Mackler et al., 2002) neurophysiology defective, with Scer\GAL4^elav.PLu, Syt1^AD4, Syt1^N13 (Poskanzer et al., 2006) neurophysiology defective, with Scer\GAL4^elav.PLu, Syt1^AD4/Syt1^N13 (Poskanzer et al., 2006) neurophysiology defective \| embryonic stage, with Scer\GAL4^elav.PLu, Syt1^AD4/Syt1^N13 (Yoshihara et al., 2010)
Phenotype Manifest In

Detailed Description
	Statement Reference Expression of Syt1[B-D3.4N.Scer\UAS] under the control of Scer\GAL4[elav.PLu] largely suppresses the asynchronous component of release seen in Syt1[N13]/Syt1[AD4] embryos when evoked release properties are recorded from muscle 6 following motor nerve stimulation in 4mM extracellular Ca[2+]. Only a small amount of residual synchronous release is seen in these embryos and the total charge transfer is dramatically reduced, similar to Syt1[N13]/Syt1[AD4] embryos. Syt1[N13]/Syt1[AD4] embryos expressing Syt1[B-D3.4N.Scer\UAS] under the control of Scer\GAL4[elav.PLu] show an enhanced rate of spontaneous release compared to controls. Embryos expressing Syt1[B-D3.4N.Scer\UAS] under the control of Scer\GAL4[elav.PLu] in a Syt1[N13]/Syt1[AD4] background show a similar level of hypertonic stimulated release as controls. (Yoshihara et al., 2010) Following a 50 Hz, 10 s stimulus, the peak ΔF/F SpH change in Syt1[AD4]/Syt1[N13]; Syt1[B-D3.4N.Scer\UAS] Scer\GAL4[elav.PLu]flies is reduced compared to controls, which agrees with prior electrophysiological demonstration of a decrease in evoked release. A slowed endocytosis rate constant is found at Syt1[B-D3.4N.Scer\UAS] rescue synapses, with an average endocytic value that is more than 2-fold slower than controls. Increasing the Ca[2+] concentration speeds the rate of endocytosis, although not to control levels. (Poskanzer et al., 2006) Larvae expressing syt^{B-D3.4N.Scer\UAS} under the control of Scer\GAL4^elav.PLu in a syt^AD4 background show a reduced mean excitatory junction potential (EJP) amplitude at the neuromuscular junction compared to syt^AD4 mutant larvae. The Ca²⁺ dependence of neurotransmitter release in larvae expressing syt^{B-D3.4N.Scer\UAS} under the control of Scer\GAL4^elav.PLu in a syt^AD4 background is significantly shifted compared to the Ca²⁺ dependence of neurotransmitter release in larvae expressing syt^Scer\UAS.cMa under the control of Scer\GAL4^elav.PLu in a syt^AD4 background. Expression of syt^{B-D3.4N.Scer\UAS} under the control of Scer\GAL4^elav.PLu in an otherwise wild-type background reduces the mean EJP amplitude at the neuromuscular junction by 53% compared to wild type. (Mackler et al., 2002)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Syt1^{B-D3.4N.Scer\UAS}/Scer\GAL4^elav.PLu partially rescues Syt1^AD4/Syt1^N13 (Yoshihara et al., 2010)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Syt1^{B-D3.4N.Scer\UAS} Syt1 <up>D416N, D418N</up> (Yoshihara et al., 2010) syt^{B-D3.4N.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Yoshihara et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(33): 14869--14874 Differential regulation of synchronous versus asynchronous neurotransmitter release by the C2 domains of synaptotagmin 1. [FBrf0211569] Poskanzer et al., 2006, Neuron 50(1): 49--62 Discrete residues in the c(2)b domain of synaptotagmin I independently specify endocytic rate and synaptic vesicle size. [FBrf0195323] Mackler et al., 2002, Nature 418(6895): 340--344 The C(2)B Ca(2+)-binding motif of synaptotagmin is required for synaptic transmission in vivo. [FBrf0151437]
Supplementary material	Mackler et al., 2002, Nature 418(6895): The C(2)B Ca(2+)-binding motif of synaptotagmin is required for synaptic transmission in vivo. [FBrf0150845]

Allele Dmel\Syt1B-D3.4N.Scer\UAS

Allele Dmel\Syt1^{B-D3.4N.Scer\UAS}