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General Information
Symbol
Dmel\shotLC.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0137087
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ΔCalp1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of a form of shot that lacks the first Calponin domain.
Lacks the F-actin-binding first Calponin domain of the shot protein.
UAS regulatory sequences drive expression of the shot L(C) isoform tagged with GFP.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of shotLC.Scer\UAS.T:Avic\GFP in embryonic motorneurons or tendon cells in a wild-type background does not engender any obvious dominant negative effects.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
The expression of shotLC.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4pnr.PU fails to rescue the increased length:width ratio of the dorsal hole during dorsal closure of shotSF20 homozygous embryos; expression under the control of Scer\GAL4en.PU rescues the long and bent microtubules at the apical of the dorsal-most epithelial cells during dorsal closure of shotSF20 homozygous embryos, but the cell body microtubules appear bundled, as compared to mutant and control embryos.
Expression of shotLC.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4elav.PU fails to rescue the increase in bouton number seen at the neuromuscular junction in shot3/shotVV third instar larvae.
Expression of shotLC.Scer\UAS.T:Avic\GFP in motorneurons fails to rescue the dendritic morphology in shotunspecified mutant embryos. Scer\GAL4sr-md710 driven expression of shotLC.Scer\UAS.T:Avic\GFP almost completely rescues the shotunspecified tendon cell phenotype.
Scer\GAL4sca-537.4-mediated expression of shotLC.Scer\UAS.T:Avic\GFP fails to restore the microtubule unbundling and axonal length phenotypes of shot3/Df(2R)MK1 primary neuronal cultures. Scer\GAL4sca-537.4-mediated expression of shotLC.Scer\UAS.T:Avic\GFP fully rescues the reduced filopodia phenotype of shot3/Df(2R)MK1 primary neuronal cultures.
(with shot<up>LA-ΔGAS2.Scer\UAS. T:Avic \GFP</up>) Fails to rescue: shot3 { Scer\GAL41407 } Expression of shotLC.Scer\UAS.T:Avic\GFP under the control of Scer\GAL41407 fails to rescue the motor and sensory axon extension defects seen in shot3 mutants. Coexpression of shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP and shotLC.Scer\UAS.T:Avic\GFP under the control of Scer\GAL41407 fails to rescue the motor and sensory axon extension defects seen in shot3 mutants.
Expression of shotLC.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4btl.PS rescues lumen formation in the tracheal dorsal trunk and at the dorsal midline in shot3 embryos.
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
shotLC.Scer\UAS.T:Avic\GFP
shotLC.UAS.GFP
Name Synonyms
Secondary FlyBase IDs
    References (8)