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General Information
Symbol
Dmel\shotLA.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0137090
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Shot L(A)-GFP, UAS-Shot L(A)-GFP, Shot::GFP, UAS-shot-GFP
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of the shot L(A) isoform tagged with GFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The expression of shotLA.Scer\UAS.T:Avic\GFP rescues both the increased length:width ratio of the dorsal hole (expression under the control of Scer\GAL4pnr.PU) and the abnormal microtubules of the dorsal-most epithelial cells (expression under the control of Scer\GAL4en.PU) observed during dorsal closure of shotSF20 homozygous embryos.

Expression of shotLA.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4elav.PU rescues the increase in bouton number seen at the neuromuscular junction in shot3/shotVV third instar larvae, while expression under the control of Scer\GAL4how-24B fails to rescue the phenotype.

Expression of shotLA.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4eve.RN2O significantly rescues the motor neuron stall phenotype seen in shotunspecified embryos in vivo while expression under the control of Scer\GAL4sca-537.4 completely rescues the axon extension and microtubule organisation defects of shotSF20/Df(2R)MK1 embryonic neurons in primary culture.

Expression of shotLA.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4sr-md710 restores tendon cell integrity in shotunspecified embryos.

Expression of shotLA.Scer\UAS.T:Avic\GFP in motorneurons rescues the severe dendritic growth phenotypes of shotunspecified embryos.

Scer\GAL4sr-md710 driven expression of shotLA.Scer\UAS.T:Avic\GFP completely rescues the shotunspecified tendon cell phenotype.

Scer\GAL4sca-537.4-mediated expression of shotLA.Scer\UAS.T:Avic\GFP fully restores the microtubule unbundling and axonal length phenotypes of shotSF20/Df(2R)MK1 primary neuronal cultures.

Scer\GAL4sca-537.4-mediated expression of shotLA.Scer\UAS.T:Avic\GFP fully rescues the reduced filopodia phenotype of shotSF20/Df(2R)MK1 primary neuronal cultures.

Expression of shotLA.Scer\UAS.T:Avic\GFP under the control of Scer\GAL41407 fully rescues the motor and sensory axon extension defects seen in shot3 mutants.

Expression of shotLA.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4btl.PS or Scer\GAL4esg-NP5130 rescues the anastomosis defects in the dorsal trunk, lateral trunk and dorsal midline seen in shot3 embryos.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
shotLA.Scer\UAS.T:Avic\GFP
shotLA.UAS.GFP
Name Synonyms
Secondary FlyBase IDs
    References (13)