FB2025_01 , released February 20, 2025
Allele: Dmel\shotLA-ΔGAS2.UAS.GFP
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General Information
Symbol
Dmel\shotLA-ΔGAS2.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0137092
Feature type
allele
Associated gene
Dmel\shot
Associated Insertion(s)
Carried in Construct
P{UAS-shot.L(A)-ΔGAS2-GFP}
Also Known As
ΔGAS2
Key Links
Transgenic product class
inframe_deletion
(Lee and Kolodziej, 2002)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Lee and Kolodziej, 2002)
Mutagen
in vitro construct
(Lee and Kolodziej, 2002, Lee and Kolodziej, 2002)
Progenitor genotype
shotLA.UAS.GFP
(Lee and Kolodziej, 2002)
Carried in construct
P{UAS-shot.L(A)-ΔGAS2-GFP}
Cytology
Description

Lacks the microtubule-binding 'Gas2' domain of the shot protein.

(Sanchez-Soriano et al., 2009)

Deletion of sequences encoding shot amino acids 4859 to 4905 (coordinates according to accession number AAF24343). This removes most of the GAS2 motif.

(Lee and Kolodziej, 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
shot
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP in embryonic motorneurons or tendon cells in a wild-type background does not engender any obvious dominant negative effects.

      (Bottenberg et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4en.PU/shotLA-ΔGAS2.UAS.GFP rescues shotSF20

      (Takács et al., 2017)

      shotLA-ΔGAS2.UAS.GFP/Scer\GAL4sca-537.4 rescues Df(2R)MK1/shotSF20

      (Sanchez-Soriano et al., 2009)

      Scer\GAL4insc-Mz1407/shotLA-ΔGAS2.UAS.GFP rescues shot3

      (Lee et al., 2007)

      shotLA-ΔGAS2.UAS.GFP/Scer\GAL4btl.PS rescues shot3

      (Lee and Kolodziej, 2002)
      Fails to rescue

      shotLA-ΔGAS2.UAS.GFP/Scer\GAL4pnr.PU fails to rescue shotSF20

      (Takács et al., 2017)

      Scer\GAL4elav.PU/shotLA-ΔGAS2.UAS.GFP fails to rescue shotVV/shot3

      (Valakh et al., 2013)

      Scer\GAL4how-24B/shotLA-ΔGAS2.UAS.GFP fails to rescue shotVV/shot3

      (Valakh et al., 2013)

      shotLA-ΔGAS2.UAS.GFP/Scer\GAL4unspecified fails to rescue shotunspecified

      (Bottenberg et al., 2009)

      Scer\GAL4sr-md710/shotLA-ΔGAS2.UAS.GFP fails to rescue shotunspecified

      (Bottenberg et al., 2009)

      shotLA-ΔGAS2.UAS.GFP/Scer\GAL4sca-537.4 fails to rescue Df(2R)MK1/shotSF20

      (Sanchez-Soriano et al., 2009)

      Scer\GAL4insc-Mz1407/shotLA-ΔGAS2.UAS.GFP fails to rescue shot3

      (Lee and Kolodziej, 2002)
      Comments

      The expression of shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP rescues the abnormal microtubules of the dorsal-most epithelial cells (expression under the control of Scer\GAL4en.PU), but not the increased length:width ratio of the dorsal hole (expression under the control of Scer\GAL4pnr.PU), observed during dorsal closure of shotSF20 homozygous embryos.

      (Takács et al., 2017)

      Expression of shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4elav.PU fails to rescue the increase in bouton number seen at the neuromuscular junction in shot3/shotVV third instar larvae.

      (Valakh et al., 2013)

      Expression of shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP in motorneurons fails to rescue the dendritic morphology in shotunspecified mutant embryos.

      Scer\GAL4sr-md710 driven expression of shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP fails to rescue the shotunspecified tendon cell phenotype.

      (Bottenberg et al., 2009)

      Scer\GAL4sca-537.4-mediated expression of shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP fails to restore the microtubule unbundling and axonal length phenotypes of shotSF20/Df(2R)MK1 primary neuronal cultures.

      Scer\GAL4sca-537.4-mediated expression of shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP rescues the reduced filopodia phenotype of shotSF20/Df(2R)MK1 primary neuronal cultures.

      (Sanchez-Soriano et al., 2009)

      (with shot<up>LC.Scer\UAS. T:Avic \GFP</up>) Fails to rescue: shot3 { Scer\GAL41407 } Expression of shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP under the control of Scer\GAL41407 fails to rescue the motor and sensory axon extension defects seen in shot3 mutants. Coexpression of shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP and shotLC.Scer\UAS.T:Avic\GFP under the control of Scer\GAL41407 fails to rescue the motor and sensory axon extension defects seen in shot3 mutants.

      (Lee and Kolodziej, 2002)

      Expression of shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4btl.PS rescues lumen formation in the tracheal dorsal trunk and at the dorsal midline in shot3 embryos.

      (Lee and Kolodziej, 2002)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      shotLA-ΔGAS2.Scer\UAS.T:Avic\GFP
      shotLA-ΔGAS2.UAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (8)