Allele Dmel\plexA^{Scer\UAS.T:Ivir\HA1}

General Information
Symbol	Dmel\plexA^{Scer\UAS.T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0137117
Feature type	allele	Associated gene	Dmel\plexA

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Terman et al., 2002, Terman et al., 2002) in vitro construct - regulatory fusion (Terman et al., 2002, Terman et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of plexA tagged with T:Ivir\HA1. (Terman et al., 2002, Terman et al., 2002)
Carried in construct	P{UAS-plexA.HA} (Terman et al., 2002, Terman et al., 2002, Ayoob et al., 2006, Sweeney et al., 2011, Hung et al., 2010, Yang and Terman, 2012)
Tagged with	T:Ivir\HA1 (Terman et al., 2002, Terman et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4^elav.PU (Yang and Terman, 2012) visible, with Scer\GAL4^B11-98 (Hung et al., 2010)
Phenotype Manifest In
	longitudinal connective, with Scer\GAL4^elav.PU (Yang and Terman, 2012) macrochaeta, with Scer\GAL4^B11-98 (Hung et al., 2010) wing disc, with Scer\GAL4^en-e16E (Sweeney et al., 2011)
Detailed Description
	Statement Reference Expression of plexA[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[elav.PU] results in axon guidance defects in the central nervous system (longitudinal connectives are disrupted). (Yang and Terman, 2012) Expression of plexA[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[en-e16E] disrupts wing disc compartment morphology. (Sweeney et al., 2011) Expression of two copies of plexA[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[B11-98] results in branched bristles. (Hung et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has neuroanatomy defective phenotype, enhanceable by 14-3-3ε^j2B10 (Yang and Terman, 2012) Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has neuroanatomy defective phenotype, enhanceable by Pka-C1^DN (Yang and Terman, 2012)
Suppressed by
Statement Reference Scer\GAL4^B11-98, plexA^{Scer\UAS.T:Ivir\HA1} has visible phenotype, suppressible by Mical^unspecified/Mical^unspecified (Hung et al., 2010) Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has neuroanatomy defective phenotype, suppressible by 14-3-3ε^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^elav.PU (Yang and Terman, 2012) Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has neuroanatomy defective phenotype, suppressible by Ras64B^{Val14.Scer\UAS}, Scer\GAL4^elav.PU (Yang and Terman, 2012) Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has neuroanatomy defective phenotype, suppressible by Scer\GAL4^elav.PU/if^Scer\UAS.cUa (Yang and Terman, 2012)
NOT suppressed by
Statement Reference Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has neuroanatomy defective phenotype, non-suppressible by Ras85D^V12.Scer\UAS, Scer\GAL4^elav.PU (Yang and Terman, 2012) Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has neuroanatomy defective phenotype, non-suppressible by Scer\GAL4^elav.PU/mew^Scer\UAS.cWa (Yang and Terman, 2012)
Suppressor of
Statement Reference plexA^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^elav-C155 is a suppressor of neuroanatomy defective phenotype of plexB^KG00878 (Ayoob et al., 2006) plexA^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^elav-C155 is a suppressor of partially lethal - majority die phenotype of plexB^KG00878 (Ayoob et al., 2006)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has embryonic/larval central nervous system phenotype, enhanceable by 14-3-3ε^j2B10 (Yang and Terman, 2012) Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has embryonic/larval central nervous system phenotype, enhanceable by Pka-C1^DN (Yang and Terman, 2012)
Suppressed by
Statement Reference Scer\GAL4^B11-98, plexA^{Scer\UAS.T:Ivir\HA1} has macrochaeta phenotype, suppressible by Mical^unspecified/Mical^unspecified (Hung et al., 2010) Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has embryonic/larval central nervous system phenotype, suppressible by 14-3-3ε^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^elav.PU (Yang and Terman, 2012) Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has embryonic/larval central nervous system phenotype, suppressible by Ras64B^{Val14.Scer\UAS}, Scer\GAL4^elav.PU (Yang and Terman, 2012) Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has embryonic/larval central nervous system phenotype, suppressible by Scer\GAL4^elav.PU/if^Scer\UAS.cUa (Yang and Terman, 2012)
NOT suppressed by
Statement Reference Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has embryonic/larval central nervous system phenotype, non-suppressible by Ras85D^V12.Scer\UAS, Scer\GAL4^elav.PU (Yang and Terman, 2012) Scer\GAL4^elav.PU, plexA^{Scer\UAS.T:Ivir\HA1} has embryonic/larval central nervous system phenotype, non-suppressible by Scer\GAL4^elav.PU/mew^Scer\UAS.cWa (Yang and Terman, 2012)
Suppressor of
Statement Reference plexA^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^elav-C155 is a suppressor of intersegmental nerve phenotype of plexB^KG00878 (Ayoob et al., 2006) plexA^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^elav-C155 is a suppressor of medial longitudinal fascicle phenotype of plexB^KG00878 (Ayoob et al., 2006) plexA^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^elav-C155 is a suppressor of segmental nerve branch SNa of A1-7 phenotype of plexB^KG00878 (Ayoob et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference The axon guidance defects in the central nervous system which are caused by expression of plexA[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[elav.PU] are enhanced if the embryos carry 14-3-3ε[j2B10] and are suppressed if the embryos also co-express 14-3-3ε[Scer\UAS.T:Zzzz\FLAG]. The axon guidance defects in the central nervous system which are caused by expression of plexA[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[elav.PU] are enhanced if the embryos carry Pka-C1[DN]. The axon guidance defects in the central nervous system which are caused by expression of plexA[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[elav.PU] are suppressed if the embryos also co-express either Ras64B[Val14.Scer\UAS] or if[Scer\UAS.cUa], but are unaffected if the embryos also co-express either Ras85D[V12.Scer\UAS] or mew[Scer\UAS.cWa]. (Yang and Terman, 2012) The branched bristle phenotype caused by expression of two copies of plexA[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[B11-98] is suppressed if the flies are also homozygous for Mical[unspecified]. (Hung et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	plexA^{Scer\UAS.T:Ivir\HA1}
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Yang and Terman, 2012, Neuron 74(1): 108--121 14-3-3ε couples protein kinase A to semaphorin signaling and silences plexin RasGAP-mediated axonal repulsion. [FBrf0218615] Sweeney et al., 2011, Neuron 72(5): 734--747 Secreted Semaphorins from Degenerating Larval ORN Axons Direct Adult Projection Neuron Dendrite Targeting. [FBrf0216932] Hung et al., 2010, Nature 463(7282): 823--827 Mical links semaphorins to F-actin disassembly. [FBrf0214846] Ayoob et al., 2006, Development 133(11): 2125--2135 Drosophila Plexin B is a Sema-2a receptor required for axon guidance. [FBrf0190270] Terman et al., 2002, Cell 109(7): 887--900 MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion. [FBrf0151231]
Supplementary material	Hung et al., 2010, Nature 463(823--827): Full Experimental Procedures. [FBrf0216820] Terman et al., 2002, Cell 109(7): MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion. [FBrf0150847]

Allele Dmel\plexAScer\UAS.T:Ivir\HA1

Allele Dmel\plexA^{Scer\UAS.T:Ivir\HA1}