FB2025_05 , released December 11, 2025
Allele: Dmel\ctUAS.cPa
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General Information
Symbol
Dmel\ctUAS.cPa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Park
FlyBase ID
FBal0137283
Feature type
allele
Associated gene
Dmel\ct
Associated Insertion(s)
Carried in Construct
P{UAS-ct.P}
Also Known As
UAS-cut, UAS-ct
Key Links
Transgenic product class
cDNA
(Hardiman et al., 2002)
wild_type
(Hardiman et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Hardiman et al., 2002)
wild_type
(Hardiman et al., 2002)
Mutagen
in vitro construct
(Hardiman et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-ct.P}
Cytology
Description

UASt regulatory sequences drive expression of the 3.4kb ct rb10 cDNA.

(Hardiman et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
ct
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal dorsal multidendritic neuron ddaD, with Scer\GAL498b

      (Lin et al., 2015)

      abdominal dorsal multidendritic neuron ddaD, with Scer\GAL4221

      (Lin et al., 2015)

      abdominal dorsal multidendritic neuron ddaE, with Scer\GAL498b

      (Lin et al., 2015)

      abdominal dorsal multidendritic neuron ddaE, with Scer\GAL4221

      (Lin et al., 2015)

      abdominal posterior ventral multidendritic neuron vdap, with Scer\GAL498b

      (Lin et al., 2015)

      abdominal posterior ventral multidendritic neuron vdap, with Scer\GAL4221

      (Lin et al., 2015, Li et al., 2004)

      abdominal posterior ventral multidendritic neuron vdap | third instar larval stage, with Scer\GAL4221

      (Nagel et al., 2012)

      actin cytoskeleton | ectopic, with Scer\GAL4salm-459.2

      (Becam et al., 2011)

      adult Malpighian tubule principal cell | ectopic | somatic clone, with Scer\GAL4NP5130

      (Xu et al., 2018)

      adult midgut | somatic clone, with Scer\GAL4NP5130

      (Xu et al., 2018)

      antennal lobe, with Scer\GAL4GH146

      (Komiyama and Luo, 2007)

      antennal lobe glomerulus DM1, with Scer\GAL4GH146

      (Komiyama and Luo, 2007)

      antennal lobe glomerulus DM2, with Scer\GAL4GH146

      (Komiyama and Luo, 2007)

      antennal lobe projection neuron, with Scer\GAL4GH146

      (Komiyama and Luo, 2007)

      dendrite, with Scer\GAL4221

      (Jinushi-Nakao et al., 2007)

      dendrite, with Scer\GAL4ppk.PG

      (Jinushi-Nakao et al., 2007)

      dendrite | larval stage, with Scer\GAL4221

      (Bhattacharjee et al., 2023)

      dorsal-ventral compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4salm-459.2

      (Becam et al., 2011)

      egg chamber | oogenesis stage S14, with Scer\GAL4GMR44E10

      (Knapp et al., 2019)

      eye, with Scer\GAL4GMR.PU

      (Javeed et al., 2015)

      larval dorsal multidendritic neuron ddaB | ectopic, with Scer\GAL4109(2)80

      (Grueber et al., 2003)

      larval dorsal multidendritic neuron ddaC, with Scer\GAL4109(2)80

      (Grueber et al., 2003)

      larval dorsal multidendritic neuron ddaC, with Scer\GAL4221

      (Jinushi-Nakao et al., 2007)

      larval dorsal multidendritic neuron ddaE, with Scer\GAL4109(2)80, Scer\GAL4IG1-2

      (Sugimura et al., 2004)

      larval multidendritic class I neuron, with Scer\GAL4221

      (Bhattacharjee et al., 2023)

      larval multidendritic class IV neuron, with Scer\GAL4ppk.PG

      (Jinushi-Nakao et al., 2007)

      multidendritic dendrite | ectopic, with Scer\GAL4109(2)80

      (Grueber et al., 2003)

      multidendritic dendrite | ectopic, with Scer\GAL4221

      (Grueber et al., 2003)

      wing, with Scer\GAL4Bx-MS1096

      (Javeed et al., 2015)

      wing margin bristle, with Scer\GAL4bbg-C96

      (Krupp et al., 2005)
      Detailed Description
      Statement
      Reference

      Females expressing ctUAS.cPa under the control of Scer\GAL4GMR44E10 lay much fewer eggs and retain significantly more mature follicles in the ovary, as compared to controls. Consistently, follicles rupture much less frequently upon induction by OA or ionomycin, as compared to controls.

      (Knapp et al., 2019)

      Midgut clones expressing ctUAS.cPa under the control of Scer\GAL4esg-NP5130 mainly contain renal progenitor/principal-like cells (i.e. Pdm1-negative, Cut-positive, polyploid cells), as compared to controls.

      (Xu et al., 2018)

      Expression of ctScer\UAS.cPa under the control of Scer\GAL4GMR.PU at 19[o]C produces flies that have pale/mottled and glazed eyes (lacking clear separation of ommatidia).

      Expression of ctScer\UAS.cPa under the control of Scer\GAL4Bx-MS1096 results in a small-wing phenotype.

      (Javeed et al., 2015)

      Scer\GAL4221 or Scer\GAL498b-mediated expression of ctScer\UAS.cPa induces dendrite overgrowth in each of the C1da neurons (vpda, ddaD, and ddaE). Scer\GAL4221-mediated expression results in 4.8 mm dendrite growth, and Scer\GAL498b results in 3.0 mm dendrite growth.

      (Lin et al., 2015)

      Expression of ctScer\UAS.cPa under the control of Scer\GAL4221 results in an increase in dendrite complexity and in the formation of spiked protrusions in class I vpda neurons.

      (Nagel et al., 2012)

      Expression of ctScer\UAS.cPa under the control of Scer\GAL4salm-459.2 induces an ectopic actin cable at the interface of the ct expressing and ct non-expressing cells.

      (Becam et al., 2011)

      Expression of ctScer\UAS.cPa in the mds neurons under the control of Scer\GAL4clh201 increases dendritic branching and causes crawling defets.

      (Hughes and Thomas, 2007)

      Ectopic expression of ctScer\UAS.cPa under the control of Scer\GAL4221 significantly increases the number of dendrite termini by 291%, total arbor length by 106%, and arbor area by 35% over wild-type levels.

      Expression of ctScer\UAS.cPa under the control of Scer\GAL4ppk.PG in class IV neurons results in the formation of only a small number of filopodia/spikes.

      (Jinushi-Nakao et al., 2007)

      In ctScer\UAS.cPa; Scer\GAL4GH146 animals there is a medial shift in targeting of both antero-dorsal (adPN) and lateral (lPN) projection neuron dendrites within the antennal lobe. adPN dendrites in these animals avoid antennal glomeruli DM1 and DM2. DL1 adPN have an abnormally diffuse dendritic arbour which targets the medial part of DL1 and adjacent glomeruli.

      (Komiyama and Luo, 2007)

      Expression of ctScer\UAS.cPa under the control of Scer\GAL4C96 disrupts wing margin bristle development.

      (Krupp et al., 2005)

      Mis-expression of ctScer\UAS.cPa, in clones under the regulation of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C during oogenesis does not direct follicle cells into either the polar-cell fate or the stalk-cell fate; instead, they appear to maintain an immature main-body follicle-cell fate. Continuous expression of ctScer\UAS.cPa under the regulation of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C during mid-oogenesis causes the follicle cells to be more densely distributed and their nuclei to be slightly smaller than those of the wild-type.

      (Sun and Deng, 2005)

      Expression of ctScer\UAS.cPa in vpda neurons, under the control of Scer\GAL4221, increases the dendritic growth and branching of these neurons.

      (Li et al., 2004)

      Expression of ctScer\UAS.cPa in ddaE neurons, under the control of Scer\GAL4109(2)80 and Scer\GAL4IG1-2, induces dendritic overgrowth and overbranching.

      (Sugimura et al., 2004)

      When ctScer\UAS.cPa is driven by Scer\GAL4221 a completely penetrant acquisition of exuberant short dendritic branches is seen in the vpda neuron. Ectopic branching is apparent by the first instar stage and persists throughout larval development. These terminal branches are highly dynamic. When ctScer\UAS.cPa is driven by Scer\GAL4109(2)80 an increase in dendritic branches is seen in ddaB neurons. When ctScer\UAS.cPa is driven by Scer\GAL4477 an decrease in dendritic branches is seen in ddaC neurons, significantly shorter terminals with both spiked and curved morphologies are seen.

      (Grueber et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Statement
      Reference

      Scer\GAL4dpp.blk1/ctUAS.cPa is a suppressor of visible | adult stage phenotype of Scer\GAL4dpp.blk1, eyUAS.cHa

      (Wang and Sun, 2012)
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of ctScer\UAS.cPa under the control of Scer\GAL4221 in a sn36a background in class I vpda neurons results in an expansion of the dendrite tree. In addition, the formation of ectopic spiked protrusions is reduced by more than 40% compared to the expression of ctScer\UAS.cPa under the control of Scer\GAL4221 in a wild-type background.

      (Nagel et al., 2012)

      Expression of ctScer\UAS.cPa almost completely suppresses the ectopic eye phenotype seen in adult flies expressing eyScer\UAS.cHa under the control of Scer\GAL4dpp.blk1. The photoreceptor induction seen in the wing discs is also suppressed.

      (Wang and Sun, 2012)

      Ectopic expression of Rac1Scer\UAS.cLa along with ctScer\UAS.cPa in class I neurons under the control of Scer\GAL4221 leads to the formation of an arbor with many filpodia/spikes, which shows a significant enhancement of branching as compared to either Rac1Scer\UAS.cLa or ctScer\UAS.cPa expression alone.

      Co-expression of knScer\UAS.cMa and ctScer\UAS.cPa under the control of Scer\GAL4221 increases the number of dendritic branching points to 409% of wild-type. Similarly, the total length is increased by 130% and the arbor area by 89%.

      Expression of ctScer\UAS.cPa under the control of Scer\GAL4ppk.PG in class IV neurons in a kn1/knKN2 results in the formation of many additional filpodia/spikes that closely resemble those of class III neurons. The overall level of branching in class IV neuron dendritic arbors appears similar to those of class III neurons.

      (Jinushi-Nakao et al., 2007)

      In acj66 homozygous somatic clones in animals carrying ctScer\UAS.cPa; Scer\GAL4GH146, DL1 adPN have diffuse dendrites which fail to innervate antennal lobe glomerulus DL1 and instead occupy the medial to dorso-medial antennal lobe: typically VM2, DM6 and DC1.

      (Komiyama and Luo, 2007)

      Coexpression of ctScer\UAS.cPa and abScer\UAS.cBa, under the control of Scer\GAL4221, does not suppress the increased dendritic branching of vpda neurons, seen when ctScer\UAS.cPa is expressed, but did partially rescue the total dendritic length phenotype.

      (Li et al., 2004)

      Coexpression of ctScer\UAS.cPa with abScer\UAS.S.cSa, under the control of Scer\GAL4109(2)80 and Scer\GAL4IG1-2, partially suppresses the reduction of field size and terminal number of ddA neurons seen when abScer\UAS.L.cSa is expressed alone. This coexpression also leads to a partial suppression of the dendritic overgrowth and overbranching of ddaE neurons, seen when ctScer\UAS.cPa is expressed under the control of Scer\GAL4109(2)80 and Scer\GAL4IG1-2.

      (Sugimura et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      ctUAS.cPa is partially rescued by ctJF03304/Scer\GAL4Bx-MS1096

      (Javeed et al., 2015)
      Partially rescues

      ctUAS.cPa/Scer\GAL4221 partially rescues ctJF03304

      (Bhattacharjee et al., 2023)

      ctUAS.cPa/Scer\GAL4GH146 partially rescues ctC145

      (Komiyama and Luo, 2007)

      ctUAS.cPa/Scer\GAL4GH146 partially rescues ctdb3

      (Komiyama and Luo, 2007)

      Scer\GAL4bbg-C96/ctUAS.cPa partially rescues ct2s

      (Krupp et al., 2005)

      Scer\GAL4bbg-C96/ctUAS.cPa partially rescues ct6

      (Krupp et al., 2005)

      Scer\GAL4bbg-C96/ctUAS.cPa partially rescues ctK

      (Krupp et al., 2005)
      Comments

      Expression of ctJF03304 partially rescues the wing phenotypes seen when ctScer\UAS.cPa is expressed under the control of Scer\GAL4Bx-MS1096, although the posterior region remains mispatterned.

      (Javeed et al., 2015)

      ctScer\UAS.cPa; Scer\GAL4GH146 completely rescues the Targeting of DMA, DM2 and VA5 antennal glomeruli by lateral projection neurons (lPN) in ctdb3 or ctC145 homozygous clones, but fails to rescue the decrease in vPN cell number and only partially rescues ventral projection neuron (vPN) Targeting of VA1lm but not of DA1.

      (Komiyama and Luo, 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      M. Park R. Bodmer

      (Hardiman et al., 2002)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      ctScer\UAS.cPa
      ctUAS.cPa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Park
      (Hardiman et al., 2002)
      Secondary FlyBase IDs
        References (27)