Allele Dmel\ct^Scer\UAS.cPa

General Information
Symbol	Dmel\ct^Scer\UAS.cPa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Park	FlyBase ID	FBal0137283
Feature type	allele	Associated gene	Dmel\ct

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Hardiman et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of the 3.4kb ct rb10 cDNA. (Hardiman et al., 2002)
Carried in construct	P{UAS-ct.P} (Krupp et al., 2005, Sun and Deng, 2005, Grueber et al., 2003, Hardiman et al., 2002, Hughes and Thomas, 2007, Sugimura et al., 2004, Li et al., 2004, Zhai et al., 2012, Zhai et al., 2011, Komiyama and Luo, 2007, Jinushi-Nakao et al., 2007, Becam et al., 2011, Wang and Sun, 2012, Nagel et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	locomotor rhythm defective, with Scer\GAL4^clh201 (Hughes and Thomas, 2007) mitotic cell cycle defective, with Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} (Sun and Deng, 2005) neuroanatomy defective, with Scer\GAL4^109(2)80, Scer\GAL4^IG1-2 (Sugimura et al., 2004) neuroanatomy defective, with Scer\GAL4²²¹ (Li et al., 2004, Jinushi-Nakao et al., 2007) neuroanatomy defective, with Scer\GAL4^clh201 (Hughes and Thomas, 2007) neuroanatomy defective, with Scer\GAL4^GH146 (Komiyama and Luo, 2007) neuroanatomy defective, with Scer\GAL4^ppk.PG (Jinushi-Nakao et al., 2007) neuroanatomy defective \| third instar larval stage, with Scer\GAL4²²¹ (Nagel et al., 2012) visible, with Scer\GAL4^bbg-C96 (Krupp et al., 2005)
Phenotype Manifest In
	abdominal posterior ventral multidendritic neuron vdap, with Scer\GAL4²²¹ (Li et al., 2004) abdominal posterior ventral multidendritic neuron vdap \| third instar larval stage, with Scer\GAL4²²¹ (Nagel et al., 2012) actin cytoskeleton \| ectopic, with Scer\GAL4^salm-459.2 (Becam et al., 2011) antennal lobe, with Scer\GAL4^GH146 (Komiyama and Luo, 2007) antennal lobe glomerulus DM1, with Scer\GAL4^GH146 (Komiyama and Luo, 2007) antennal lobe glomerulus DM2, with Scer\GAL4^GH146 (Komiyama and Luo, 2007) antennal lobe projection neuron, with Scer\GAL4^GH146 (Komiyama and Luo, 2007) class IV dendritic arborizing neuron, with Scer\GAL4^ppk.PG (Jinushi-Nakao et al., 2007) dendrite, with Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) dendrite, with Scer\GAL4^ppk.PG (Jinushi-Nakao et al., 2007) dorsal multidendritic neuron ddaB \| ectopic, with Scer\GAL4^109(2)80 (Grueber et al., 2003) dorsal multidendritic neuron ddaC, with Scer\GAL4^109(2)80 (Grueber et al., 2003) dorsal multidendritic neuron ddaC, with Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) dorsal multidendritic neuron ddaE, with Scer\GAL4^109(2)80, Scer\GAL4^IG1-2 (Sugimura et al., 2004) dorsal-ventral compartment boundary of the wing \| third instar larval stage, with Scer\GAL4^salm-459.2 (Becam et al., 2011) multidendritic dendrite \| ectopic, with Scer\GAL4^109(2)80 (Grueber et al., 2003) multidendritic dendrite \| ectopic, with Scer\GAL4²²¹ (Grueber et al., 2003) wing margin bristle, with Scer\GAL4^bbg-C96 (Krupp et al., 2005)
Detailed Description
	Statement Reference Expression of ct[Scer\UAS.cPa] under the control of Scer\GAL4[221] results in an increase in dendrite complexity and in the formation of spiked protrusions in class I vpda neurons. (Nagel et al., 2012) Expression of ct[Scer\UAS.cPa] under the control of Scer\GAL4[salm-459.2] induces an ectopic actin cable at the interface of the ct expressing and ct non-expressing cells. (Becam et al., 2011) Expression of ct[Scer\UAS.cPa] in the mds neurons under the control of Scer\GAL4[clh201] increases dendritic branching and causes crawling defets. (Hughes and Thomas, 2007) Ectopic expression of ct[Scer\UAS.cPa] under the control of Scer\GAL4[221] significantly increases the number of dendrite termini by 291%, total arbor length by 106%, and arbor area by 35% over wild-type levels. Expression of ct[Scer\UAS.cPa] under the control of Scer\GAL4[ppk.PG] in class IV neurons results in the formation of only a small number of filopodia/spikes. (Jinushi-Nakao et al., 2007) In ct[Scer\UAS.cPa]; Scer\GAL4[GH146] animals there is a medial shift in targeting of both antero-dorsal (adPN) and lateral (lPN) projection neuron dendrites within the antennal lobe. adPN dendrites in these animals avoid antennal glomeruli DM1 and DM2. DL1 adPN have an abnormally diffuse dendritic arbour which targets the medial part of DL1 and adjacent glomeruli. (Komiyama and Luo, 2007) Expression of ct^Scer\UAS.cPa under the control of Scer\GAL4^C96 disrupts wing margin bristle development. (Krupp et al., 2005) Mis-expression of ct^Scer\UAS.cPa, in clones under the regulation of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} during oogenesis does not direct follicle cells into either the polar-cell fate or the stalk-cell fate; instead, they appear to maintain an immature main-body follicle-cell fate. Continuous expression of ct^Scer\UAS.cPa under the regulation of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} during mid-oogenesis causes the follicle cells to be more densely distributed and their nuclei to be slightly smaller than those of the wild-type. (Sun and Deng, 2005) Expression of ct^Scer\UAS.cPa in vpda neurons, under the control of Scer\GAL4²²¹, increases the dendritic growth and branching of these neurons. (Li et al., 2004) Expression of ct^Scer\UAS.cPa in ddaE neurons, under the control of Scer\GAL4^109(2)80 and Scer\GAL4^IG1-2, induces dendritic overgrowth and overbranching. (Sugimura et al., 2004) When ct^Scer\UAS.cPa is driven by Scer\GAL4²²¹ a completely penetrant acquisition of exuberant short dendritic branches is seen in the vpda neuron. Ectopic branching is apparent by the first instar stage and persists throughout larval development. These terminal branches are highly dynamic. When ct^Scer\UAS.cPa is driven by Scer\GAL4^109(2)80 an increase in dendritic branches is seen in ddaB neurons. When ct^Scer\UAS.cPa is driven by Scer\GAL4⁴⁷⁷ an decrease in dendritic branches is seen in ddaC neurons, significantly shorter terminals with both spiked and curved morphologies are seen. (Grueber et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4²²¹, ct^Scer\UAS.cPa has neuroanatomy defective phenotype, enhanceable by kn^Scer\UAS.cMa/Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) Scer\GAL4²²¹, ct^Scer\UAS.cPa has neuroanatomy defective phenotype, enhanceable by Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007)
Suppressed by
Statement Reference Scer\GAL4^109(2)80, Scer\GAL4^IG1-2, ct^Scer\UAS.cPa has neuroanatomy defective phenotype, suppressible \| partially by Scer\GAL4^109(2)80/ab^{Scer\UAS.S.cSa}, Scer\GAL4^IG1-2 (Sugimura et al., 2004)
Enhancer of
Statement Reference ct^Scer\UAS.cPa, Scer\GAL4²²¹ is an enhancer of neuroanatomy defective phenotype of Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) ct^Scer\UAS.cPa/Scer\GAL4²²¹ is an enhancer of neuroanatomy defective phenotype of Scer\GAL4²²¹, kn^Scer\UAS.cMa (Jinushi-Nakao et al., 2007) ct^Scer\UAS.cPa/Scer\GAL4^ppk.PG is an enhancer of neuroanatomy defective \| somatic clone phenotype of kn^KN2/kn¹ (Jinushi-Nakao et al., 2007)
Suppressor of
Statement Reference Scer\GAL4^dpp.blk1/ct^Scer\UAS.cPa is a suppressor of visible \| adult stage phenotype of Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa (Wang and Sun, 2012)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4²²¹, ct^Scer\UAS.cPa has class I dendritic arborizing neuron phenotype, enhanceable by kn^Scer\UAS.cMa/Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) Scer\GAL4²²¹, ct^Scer\UAS.cPa has class I dendritic arborizing neuron phenotype, enhanceable by Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) Scer\GAL4²²¹, ct^Scer\UAS.cPa has dendrite phenotype, enhanceable by kn^Scer\UAS.cMa/Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) Scer\GAL4²²¹, ct^Scer\UAS.cPa has dendrite phenotype, enhanceable by Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007)
Suppressed by
Statement Reference Scer\GAL4^109(2)80, Scer\GAL4^IG1-2, ct^Scer\UAS.cPa has dorsal multidendritic neuron ddaE phenotype, suppressible \| partially by Scer\GAL4^109(2)80/ab^{Scer\UAS.S.cSa}, Scer\GAL4^IG1-2 (Sugimura et al., 2004) Scer\GAL4²²¹, ct^Scer\UAS.cPa has abdominal posterior ventral multidendritic neuron vdap phenotype, suppressible \| partially by ab^Scer\UAS.cBa/Scer\GAL4²²¹ (Li et al., 2004)
Enhancer of
Statement Reference ct^Scer\UAS.cPa, Scer\GAL4²²¹ is an enhancer of class I dendritic arborizing neuron phenotype of Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) ct^Scer\UAS.cPa, Scer\GAL4²²¹ is an enhancer of dendrite phenotype of Rac1^Scer\UAS.cLa, Scer\GAL4²²¹ (Jinushi-Nakao et al., 2007) ct^Scer\UAS.cPa/Scer\GAL4²²¹ is an enhancer of class I dendritic arborizing neuron phenotype of Scer\GAL4²²¹, kn^Scer\UAS.cMa (Jinushi-Nakao et al., 2007) ct^Scer\UAS.cPa/Scer\GAL4²²¹ is an enhancer of dendrite phenotype of Scer\GAL4²²¹, kn^Scer\UAS.cMa (Jinushi-Nakao et al., 2007) ct^Scer\UAS.cPa/Scer\GAL4^GH146 is an enhancer of adult antennal lobe projection neuron DL1 adPN \| somatic clone phenotype of acj6⁶ (Komiyama and Luo, 2007) ct^Scer\UAS.cPa/Scer\GAL4^GH146 is an enhancer of antennal lobe glomerulus DL1 \| somatic clone phenotype of acj6⁶ (Komiyama and Luo, 2007) ct^Scer\UAS.cPa/Scer\GAL4^ppk.PG is an enhancer of class IV dendritic arborizing neuron \| somatic clone phenotype of kn^KN2/kn¹ (Jinushi-Nakao et al., 2007) ct^Scer\UAS.cPa/Scer\GAL4^ppk.PG is an enhancer of dendrite \| somatic clone phenotype of kn^KN2/kn¹ (Jinushi-Nakao et al., 2007) ct^Scer\UAS.cPa/Scer\GAL4^ppk.PG is an enhancer of dorsal multidendritic neuron ddaC \| somatic clone phenotype of kn^KN2/kn¹ (Jinushi-Nakao et al., 2007)
Suppressor of
Statement Reference Scer\GAL4^109(2)80/ct^Scer\UAS.cPa, Scer\GAL4^IG1-2 is a suppressor of dorsal multidendritic neuron ddaA phenotype of Scer\GAL4^109(2)80, Scer\GAL4^IG1-2, ab^{Scer\UAS.S.cSa} (Sugimura et al., 2004) Scer\GAL4^dpp.blk1/ct^Scer\UAS.cPa is a suppressor of eye \| ectopic phenotype of Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa (Wang and Sun, 2012) Scer\GAL4^dpp.blk1/ct^Scer\UAS.cPa is a suppressor of photoreceptor \| ectopic \| third instar larval stage phenotype of Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa (Wang and Sun, 2012)
Other
Statement Reference Scer\GAL4^GH146, acj6⁶, ct^Scer\UAS.cPa, vvl^Scer\UAS.cCa has antennal lobe glomerulus DA2 \| somatic clone phenotype (Komiyama and Luo, 2007) Scer\GAL4^GH146, acj6⁶, ct^Scer\UAS.cPa, vvl^Scer\UAS.cCa has antennal lobe glomerulus DM2 \| somatic clone phenotype (Komiyama and Luo, 2007) Scer\GAL4^GH146, acj6⁶, ct^Scer\UAS.cPa has antennal lobe glomerulus DC1 \| somatic clone phenotype (Komiyama and Luo, 2007) Scer\GAL4^GH146, acj6⁶, ct^Scer\UAS.cPa has antennal lobe glomerulus DM6 \| somatic clone phenotype (Komiyama and Luo, 2007) Scer\GAL4^GH146, acj6⁶, ct^Scer\UAS.cPa has antennal lobe glomerulus VM2 \| somatic clone phenotype (Komiyama and Luo, 2007)
Additional Comments
Genetic Interactions
Statement Reference Expression of ct[Scer\UAS.cPa] under the control of Scer\GAL4[221] in a sn[36a] background in class I vpda neurons results in an expansion of the dendrite tree. In addition, the formation of ectopic spiked protrusions is reduced by more than 40% compared to the expression of ct[Scer\UAS.cPa] under the control of Scer\GAL4[221] in a wild-type background. (Nagel et al., 2012) Expression of ct[Scer\UAS.cPa] almost completely suppresses the ectopic eye phenotype seen in adult flies expressing ey[Scer\UAS.cHa] under the control of Scer\GAL4[dpp.blk1]. The photoreceptor induction seen in the wing discs is also suppressed. (Wang and Sun, 2012) Ectopic expression of Rac1[Scer\UAS.cLa] along with ct[Scer\UAS.cPa] in class I neurons under the control of Scer\GAL4[221] leads to the formation of an arbor with many filpodia/spikes, which shows a significant enhancement of branching as compared to either Rac1[Scer\UAS.cLa] or ct[Scer\UAS.cPa] expression alone. Co-expression of kn[Scer\UAS.cMa] and ct[Scer\UAS.cPa] under the control of Scer\GAL4[221] increases the number of dendritic branching points to 409% of wild-type. Similarly, the total length is increased by 130% and the arbor area by 89%. Expression of ct[Scer\UAS.cPa] under the control of Scer\GAL4[ppk.PG] in class IV neurons in a kn[1]/kn[KN2] results in the formation of many additional filpodia/spikes that closely resemble those of class III neurons. The overall level of branching in class IV neuron dendritic arbors appears similar to those of class III neurons. (Jinushi-Nakao et al., 2007) In acj6[6] homozygous somatic clones in animals carrying ct[Scer\UAS.cPa]; Scer\GAL4[GH146], DL1 adPN have diffuse dendrites which fail to innervate antennal lobe glomerulus DL1 and instead occupy the medial to dorso-medial antennal lobe: typically VM2, DM6 and DC1. (Komiyama and Luo, 2007) Coexpression of ct^Scer\UAS.cPa and ab^Scer\UAS.cBa, under the control of Scer\GAL4²²¹, does not suppress the increased dendritic branching of vpda neurons, seen when ct^Scer\UAS.cPa is expressed, but did partially rescue the total dendritic length phenotype. (Li et al., 2004) Coexpression of ct^Scer\UAS.cPa with ab^{Scer\UAS.S.cSa}, under the control of Scer\GAL4^109(2)80 and Scer\GAL4^IG1-2, partially suppresses the reduction of field size and terminal number of ddA neurons seen when ab^{Scer\UAS.L.cSa} is expressed alone. This coexpression also leads to a partial suppression of the dendritic overgrowth and overbranching of ddaE neurons, seen when ct^Scer\UAS.cPa is expressed under the control of Scer\GAL4^109(2)80 and Scer\GAL4^IG1-2. (Sugimura et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	ct^Scer\UAS.cPa/Scer\GAL4^GH146 partially rescues ct^C145 (Komiyama and Luo, 2007) ct^Scer\UAS.cPa/Scer\GAL4^GH146 partially rescues ct^db3 (Komiyama and Luo, 2007) Scer\GAL4^bbg-C96/ct^Scer\UAS.cPa partially rescues ct^2s (Krupp et al., 2005) Scer\GAL4^bbg-C96/ct^Scer\UAS.cPa partially rescues ct⁶ (Krupp et al., 2005) Scer\GAL4^bbg-C96/ct^Scer\UAS.cPa partially rescues ct^K (Krupp et al., 2005)
Comments	ct[Scer\UAS.cPa]; Scer\GAL4[GH146] completely rescues the Targeting of DMA, DM2 and VA5 antennal glomeruli by lateral projection neurons (lPN) in ct[db3] or ct[C145] homozygous clones, but fails to rescue the decrease in vPN cell number and only partially rescues ventral projection neuron (vPN) Targeting of VA1lm but not of DA1. (Komiyama and Luo, 2007)
Stocks ( 1 )
Bloomington	36496 w^* ct^C145 P{neoFRT}19A/FM7c; P{UAS-ct.P}2, P{UAS-mCD8::GFP.L}LL5/CyO
Notes on Origin
Discoverer	M. Park R. Bodmer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ct^Scer\UAS.cPa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Park (Hardiman et al., 2002)
Secondary FlyBase IDs

References ( 14 )
Research paper	Nagel et al., 2012, Development 139(16): 2999--3009 Fascin controls neuronal class-specific dendrite arbor morphology. [FBrf0219030] Wang and Sun, 2012, Development 139(18): 3413--3421 Segregation of eye and antenna fates maintained by mutual antagonism in Drosophila. [FBrf0219201] Zhai et al., 2012, PLoS Genet. 8(3): e1002582 Antagonistic regulation of apoptosis and differentiation by the cut transcription factor represents a tumor-suppressing mechanism in Drosophila. [FBrf0217859] Becam et al., 2011, Development 138(17): 3781--3789 Notch-mediated repression of bantam miRNA contributes to boundary formation in the Drosophila wing. [FBrf0214603] Zhai et al., 2011, Biochem. Biophys. Res. Commun. 414(4): 761--766 Functional dissection of the Hox protein Abdominal-B in Drosophila cell culture. [FBrf0216579] Hughes and Thomas, 2007, Mol. Cell. Neurosci. 35(2): 383--396 A sensory feedback circuit coordinates muscle activity in Drosophila. [FBrf0201151] Jinushi-Nakao et al., 2007, Neuron 56(6): 963--978 Knot/Collier and cut control different aspects of dendrite cytoskeleton and synergize to define final arbor shape. [FBrf0202055] Komiyama and Luo, 2007, Curr. Biol. 17(3): 278--285 Intrinsic control of precise dendritic targeting by an ensemble of transcription factors. [FBrf0195086] Krupp et al., 2005, Genetics 170(4): 1775--1795 Identification of genetic loci that interact with cut during Drosophila wing-margin development. [FBrf0187644] Sun and Deng, 2005, Development 132(19): 4299--4308 Notch-dependent downregulation of the homeodomain gene cut is required for the mitotic cycle/endocycle switch and cell differentiation in Drosophila follicle cells. [FBrf0187484] Li et al., 2004, Neuron 43(6): 823--834 BTB/POZ-zinc finger protein abrupt suppresses dendritic branching in a neuronal subtype-specific and dosage-dependent manner. [FBrf0180577] Sugimura et al., 2004, Neuron 43(6): 809--822 Development of morphological diversity of dendrites in Drosophila by the BTB-zinc finger protein abrupt. [FBrf0180576] Grueber et al., 2003, Cell 112(6): 805--818 Different levels of the homeodomain protein Cut regulate distinct dendrite branching patterns of Drosophila multidendritic neurons. [FBrf0157166] Hardiman et al., 2002, Genetics 161(1): 231--247 The bereft gene, a potential target of the neural selector gene cut, contributes to bristle morphogenesis. [FBrf0149016]

Allele Dmel\ctScer\UAS.cPa

Allele Dmel\ct^Scer\UAS.cPa