Allele Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}

General Information
Symbol	Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}	Species	a. artificial
Name		FlyBase ID	FBal0137335
Feature type	allele	Associated gene	Zzzz\CAG

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Marsh et al., 2000) in vitro construct - regulatory fusion (Kazantsev et al., 2002, Marsh et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a polyglutamine repeat tagged with T:Hsap\MYC and T:Zzzz\FLAG. (Kazantsev et al., 2002) Scer\UAS regulatory sequences drive expression of a 108 amino acid polyglutamine repeat (the repeat is flanked by the amino acid sequence MRSRKL at the N-terminal end and by KLRS at the C-terminal end). The T:Hsap\MYC and T:Zzzz\FLAG tags are present at the C-terminal end of the open reading frame (immediately after the KLRS sequence). (Marsh et al., 2000)
Carried in construct	P{UAS-Q108.myc.flag} (Kazantsev et al., 2002, Marsh et al., 2000, Berger et al., 2005, Iijima-Ando et al., 2010)
Tagged with	T:Hsap\MYC (Marsh et al., 2000, Kazantsev et al., 2002) T:Zzzz\FLAG (Marsh et al., 2000, Kazantsev et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	eye color defective, with Scer\GAL4^GMR.PF (Marsh et al., 2000) partially lethal - majority die, with Scer\GAL4^elav-C155 (Berger et al., 2005) partially lethal - majority die, with Scer\GAL4^GMR.PF (Marsh et al., 2000) partially lethal - majority die, with Scer\GAL4^hs.2sev (Marsh et al., 2000) short lived, with Scer\GAL4^elav.PU (Marsh et al., 2000) viable, with Scer\GAL4^dpp.blk1 (Marsh et al., 2000) viable, with Scer\GAL4^elav.PU (Marsh et al., 2000) visible, with Scer\GAL4^dpp.blk1 (Marsh et al., 2000) visible, with Scer\GAL4^GMR.PF (Marsh et al., 2000) visible, with Scer\GAL4^hs.2sev (Marsh et al., 2000) visible \| adult stage, with Scer\GAL4^GMR.PF (Iijima-Ando et al., 2010)
Phenotype Manifest In
	adult thorax, with Scer\GAL4^dpp.blk1 (Marsh et al., 2000) eye, with Scer\GAL4^GMR.PF (Marsh et al., 2000, Iijima-Ando et al., 2010) macrochaeta, with Scer\GAL4^hs.2sev (Marsh et al., 2000) retina, with Scer\GAL4^elav.PU (Marsh et al., 2000) rhabdomere, with Scer\GAL4^elav.PLu (Kazantsev et al., 2002) rhabdomere, with Scer\GAL4^elav.PU (Marsh et al., 2000)
Detailed Description
	Statement Reference Expression of Zzzz\CAG[Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] in the eye under the control of Scer\GAL4[GMR.PF] causes depigmentation. (Iijima-Ando et al., 2010) Expression of Zzzz\CAG[Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[elav-C155] results in dramatic pre-adult lethality, which is partially rescued by Li[+]. (Berger et al., 2005) Expression of Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PLu results in degeneration of neurons in the eye, as indicated by large gaps in the eye tissue and loss of rhabdomeres. Each ommatidium contains on average only 3 neurons in these eyes (compared to 7 in wild type). (Kazantsev et al., 2002) Adult survivors expressing Zzzz\CAG[Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[dpp.blk1] often have a split thorax phenotype. Adult survivors expressing Zzzz\CAG[Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[GMR.PF] have a rough eye with no pigment. Adult survivors expressing Zzzz\CAG[Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[hs.2sev] show a loss of large sensory bristles. External eye morphology is normal in adults expressing Zzzz\CAG[Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[elav.PU]. However, many of the ommatidia contain only four to six rhabdomeres. Gaps are evident in the retinal tissue. Adults expressing Zzzz\CAG[Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[elav.PU] have a shortened lifespan compared to wild type. (Marsh et al., 2000)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PF, Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has visible \| adult stage phenotype, non-enhanceable by Scer\GAL4^GMR.PF/lok^Scer\UAS.cPa (Iijima-Ando et al., 2010)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PF, Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye phenotype, non-enhanceable by Scer\GAL4^GMR.PF/lok^Scer\UAS.cPa (Iijima-Ando et al., 2010)
Suppressed by
Statement Reference Scer\GAL4^elav.PLu, Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has rhabdomere phenotype, suppressible by Hsap\HTT^{Su3.Scer\UAS.T:Zzzz\CAG,T:Hsap\MYC}, Scer\GAL4^elav.PLu (Kazantsev et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of lok[Scer\UAS.cPa] does not enhance the eye depigmentation phenotype seen when Zzzz\CAG[Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] is expressed under the control of Scer\GAL4[GMR.PF]. (Iijima-Ando et al., 2010) Co-expression of Hsap\HD^{Su3.Scer\UAS.T:Zzzz\CAG,T:Hsap\MYC} significantly suppresses the loss of photoreceptor neurons seen in flies expressing Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PLu. (Kazantsev et al., 2002)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Iijima-Ando et al., 2010, Hum. Mol. Genet. 19(10): 1930--1938 A DNA damage-activated checkpoint kinase phosphorylates tau and enhances tau-induced neurodegeneration. [FBrf0210684] Berger et al., 2005, Hum. Mol. Genet. 14(20): 3003--3011 Lithium rescues toxicity of aggregate-prone proteins in Drosophila by perturbing Wnt pathway. [FBrf0190825] Kazantsev et al., 2002, Nat. Genet. 30(4): 367--376 A bivalent Huntingtin binding peptide suppresses polyglutamine aggregation and pathogenesis in Drosophila. [FBrf0147171] Marsh et al., 2000, Hum. Mol. Genet. 9(1): 13--25 Expanded polyglutamine peptides alone are intrinsically cytotoxic and cause neurodegeneration in Drosophila. [FBrf0123123]

Allele Zzzz\CAGQ108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG

Allele Zzzz\CAG^{Q108.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}