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General Information
Symbol
Dmel\Rap1P5709
Species
D. melanogaster
Name
FlyBase ID
FBal0137389
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P-element insertion in the R coding region.

Insertion components
P{}Rap1P5709
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Zygotic RP5709 mutant embryos show a very slight macrophage migration phenotype. After germ band retraction, the migration along the midline of the ventral nerve cord is slightly disturbed, resulting in a small area that is free of macrophages at stage 14. The size of macrophage protrusions is not affected.

Homozygous clones in the pupal wing intermingle with surrounding wild-type cells (in contrast to wild-type clones which have discrete borders). The cells are often dispersed into wild-type tissue in pairs or groups of 4 cells. The mutant cells still respect the lineage restriction at the anterior-posterior boundary. The mutant cells are not the normal hexagonal shape and the area of the apical, but not the basal, surface of the cells is reduced relative to wild-type wing cells. 82% of mutant cells in clones in the pupal wing have adherens junctions condensed into a contact with just one neighbouring cell, in contrast to wild type where there is an even distribution of adherens junction components around the apical circumference of the cell. Clusters of adherens junctions are seen only at interfaces between mutant cells, and not between a mutant and a wild-type cell. Homozygous clones in late third larval instar wing discs are not dispersed, but adherens junctions components are mislocalised, indicating adherens junction mislocalisation precedes dispersal. Dispersal of the mutant cells is first seen 2 hours after pupariation and is initially more pronounced at the distal end of the evaginating wing.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement
Reference
Phenotype Manifest In
Suppressor of
Statement
Reference
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The proportion of hemisegments with abnormal number of neurons in the asymmetrically dividing RP2 neural lineage is significantly increased in wtsx1/+;Rap1P5709/+ double heterozygous embryos compared to either of the single heterozygotes or wild type.

The cell shape changes and protrusion network formation phenotype of macrophages expressing Gef26EP388 under the control of Scer\GAL4srp is substantially rescued in RP5709 mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (4)