Zygotic RP5709 mutant embryos show a very slight macrophage migration phenotype. After germ band retraction, the migration along the midline of the ventral nerve cord is slightly disturbed, resulting in a small area that is free of macrophages at stage 14. The size of macrophage protrusions is not affected.
Homozygous clones in the pupal wing intermingle with surrounding wild-type cells (in contrast to wild-type clones which have discrete borders). The cells are often dispersed into wild-type tissue in pairs or groups of 4 cells. The mutant cells still respect the lineage restriction at the anterior-posterior boundary. The mutant cells are not the normal hexagonal shape and the area of the apical, but not the basal, surface of the cells is reduced relative to wild-type wing cells. 82% of mutant cells in clones in the pupal wing have adherens junctions condensed into a contact with just one neighbouring cell, in contrast to wild type where there is an even distribution of adherens junction components around the apical circumference of the cell. Clusters of adherens junctions are seen only at interfaces between mutant cells, and not between a mutant and a wild-type cell. Homozygous clones in late third larval instar wing discs are not dispersed, but adherens junctions components are mislocalised, indicating adherens junction mislocalisation precedes dispersal. Dispersal of the mutant cells is first seen 2 hours after pupariation and is initially more pronounced at the distal end of the evaginating wing.