Allele Dmel\Liprin-α^F3ex15

General Information
Symbol	Dmel\Liprin-α^F3ex15	Species	D. melanogaster
Name		FlyBase ID	FBal0137468
Feature type	allele	Associated gene	Dmel\Liprin-α
Also Known As	dliprin-α^F3ex15

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Kaufmann et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{lacW}Liprin-α^F3 (Kaufmann et al., 2002)
Nature of the lesion	Statement Reference Imprecise excision of the P{lacW} element, removing the Liprin-α coding region. Some of the Liprin-α 5' UTR and some P-element sequences are still present. (Kaufmann et al., 2002)
Caused by insertion	P{?lacW}Liprin-α^F3ex15 (Kaufmann et al., 2002, Miller, 2005)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective (with Liprin-α^R60) (Kaufmann et al., 2002) neuroanatomy defective \| third instar larval stage (with Liprin-α^R60) (Owald et al., 2010) neurophysiology defective (with Liprin-α^1J-0) (Miller et al., 2005) neurophysiology defective (with Liprin-α^R117) (Kaufmann et al., 2002)
Phenotype Manifest In
	bouton (with Liprin-α^R60) (Kaufmann et al., 2002) embryonic/larval neuromuscular junction \| third instar larval stage (with Liprin-α^R60) (Owald et al., 2010) neuromuscular junction (with Liprin-α^R60) (Kaufmann et al., 2002) neuromuscular junction (with Liprin-α^R117) (Kaufmann et al., 2002) synaptic vesicle (with Liprin-α^1J-0) (Miller et al., 2005)
Detailed Description
	Statement Reference Liprin-α[F3ex15]/Liprin-α[R60] adults do not show defects in locomotion (assayed both by walking ability on a flat surface and in a negative geotaxis assay). Evoked excitatory junctional currents (EJCs) are significantly reduced in amplitude in Liprin-α[F3ex15]/Liprin-α[R60] third instar larvae compared to controls, while the amplitude of spontaneous miniature EJCs is normal. The overall size of the neuromuscular junction is reduced in Liprin-α[F3ex15]/Liprin-α[R60] third instar larvae compared to controls. (Owald et al., 2010) Mutant neuromuscular junctions show reliable homeostatic compensation (increase in quantal content) after treatment with philanthotoxin-433 for 10 minutes. (Dickman and Davis, 2009) Liprin-α^F3ex15/Liprin-α^1J-0 mutant peripheral nerves have an accumulation of clear-core vesicles along the axon, compared to wild-type axons. Visualization of synaptic vesicle precursor transport along motor axons in intact third instar larva shows that the flux of cargo in the anterograde direction is significantly decreased in the mutants, while the flux is increased in the retrograde direction. However, the total flux is unchanged. There are no ectopic presynaptic specializations, breakdowns in the plasma membrane, or obvious changes in microtubule density or morphology along motor axons of Liprin-α^F3ex15/Liprin-α^1J-0 mutants. (Miller et al., 2005) Liprin-α^F3ex15/Df(2L)Liprin-α-77ex63 embryos do not show defects in motor axon pathfinding. Liprin-α^F3ex15/Liprin-α^R60 larval neuromuscular junctions frequently lack small nascent boutons ("buds") surrounding a large end bouton (which are seen in wild-type animals). Overall neuromuscular junction ultrastructure looks relatively normal in Liprin-α^F3ex15/Liprin-α^R60 larvae, but active zones are always abnormal in either total size or shape, with the mean maximum dimension being nearly double that of wild type. Excitatory junctional potentials (EJPs) are reduced 36% in Liprin-α^F3ex15/Liprin-α^R117 larvae compared to controls. The mean amplitude and the frequency of unitary mRJPs is normal. The resting potential of the muscle is normal. The quantal content of evoked release is reduced by over 50%. (Kaufmann et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Df(3R)RhoGAP100F^ex1.2/Df(3R)RhoGAP100F^ex3.4, Liprin-α^R60/Liprin-α^F3ex15 has lethal \| embryonic stage phenotype (Owald et al., 2010)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	8563 Liprin-α^F3ex15/In(2LR)Gla, wg^Gla-1 Bc¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	dliprin-α^F3ex15 (Fouquet et al., 2009) DLiprin-α^F3ex15 (Owald et al., 2010) Liprin-α^F3ex15 lip-α^F3ex5 (Johnson et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Owald et al., 2010, J. Cell Biol. 188(4): 565--579 A Syd-1 homologue regulates pre- and postsynaptic maturation in Drosophila. [FBrf0210057] Dickman and Davis, 2009, Science 326(5956): 1127--1130 The schizophrenia susceptibility gene dysbindin controls synaptic homeostasis. [FBrf0209454] Fouquet et al., 2009, J. Cell Biol. 186(1): 129--145 Maturation of active zone assembly by Drosophila Bruchpilot. [FBrf0208461] Johnson et al., 2009, PLoS Biol. 7(9): e1000193 Negative regulation of active zone assembly by a newly identified SR protein kinase. [FBrf0208771] Miller et al., 2005, Curr. Biol. 15(7): 684--689 Direct observation demonstrates that Liprin-alpha is required for trafficking of synaptic vesicles. [FBrf0187322] Kaufmann et al., 2002, Neuron 34(1): 27--38 Drosophila liprin-alpha and the receptor phosphatase Dlar control synapse morphogenesis. [FBrf0147174]
Supplementary material	Miller, 2005, Curr. Biol. 15(7): Document S1. Supplemental Experimental Procedures. [FBrf0191731]

Allele Dmel\Liprin-αF3ex15

Allele Dmel\Liprin-α^F3ex15