|Feature type||allele||Associated gene||Dmel\Liprin-α|
|Also Known As||dliprin-αF3ex15|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Caused by insertion|
|Phenotype Manifest In|
Liprin-α[F3ex15]/Liprin-α[R60] adults do not show defects in locomotion (assayed both by walking ability on a flat surface and in a negative geotaxis assay). Evoked excitatory junctional currents (EJCs) are significantly reduced in amplitude in Liprin-α[F3ex15]/Liprin-α[R60] third instar larvae compared to controls, while the amplitude of spontaneous miniature EJCs is normal. The overall size of the neuromuscular junction is reduced in Liprin-α[F3ex15]/Liprin-α[R60] third instar larvae compared to controls.
Mutant neuromuscular junctions show reliable homeostatic compensation (increase in quantal content) after treatment with philanthotoxin-433 for 10 minutes.
Liprin-αF3ex15/Liprin-α1J-0 mutant peripheral nerves have an accumulation of clear-core vesicles along the axon, compared to wild-type axons. Visualization of synaptic vesicle precursor transport along motor axons in intact third instar larva shows that the flux of cargo in the anterograde direction is significantly decreased in the mutants, while the flux is increased in the retrograde direction. However, the total flux is unchanged. There are no ectopic presynaptic specializations, breakdowns in the plasma membrane, or obvious changes in microtubule density or morphology along motor axons of Liprin-αF3ex15/Liprin-α1J-0 mutants.
Liprin-αF3ex15/Df(2L)Liprin-α-77ex63 embryos do not show defects in motor axon pathfinding. Liprin-αF3ex15/Liprin-αR60 larval neuromuscular junctions frequently lack small nascent boutons ("buds") surrounding a large end bouton (which are seen in wild-type animals). Overall neuromuscular junction ultrastructure looks relatively normal in Liprin-αF3ex15/Liprin-αR60 larvae, but active zones are always abnormal in either total size or shape, with the mean maximum dimension being nearly double that of wild type. Excitatory junctional potentials (EJPs) are reduced 36% in Liprin-αF3ex15/Liprin-αR117 larvae compared to controls. The mean amplitude and the frequency of unitary mRJPs is normal. The resting potential of the muscle is normal. The quantal content of evoked release is reduced by over 50%.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 1 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 4 )|
|Secondary FlyBase IDs|
|References ( 7 )|