FlyBase Allele Report: Dmel\Liprin-α[1J-0]

General Information

Symbol

Dmel\Liprin-α^1J-0

Species

D. melanogaster

Name

FlyBase ID

FBal0137470

Feature type

allele

Associated gene

Dmel\Liprin-α

Associated Insertion(s)

P{lacW}Liprin-α^1J-0

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Kaufmann et al., 2002)

Progenitor genotype

Associated Insertion(s)

P{lacW}Liprin-α^1J-0

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Liprin-α^R117)

(Kaufmann et al., 2002)

abnormal neurophysiology (with Liprin-α^F3ex15)

(Miller et al., 2005)

Phenotype Manifest In

bouton (with Liprin-α^R117)

(Kaufmann et al., 2002)

neuromuscular junction (with Liprin-α^R117)

(Kaufmann et al., 2002)

synaptic vesicle (with Liprin-α^F3ex15)

(Miller et al., 2005)

Detailed Description

Statement

Reference

Liprin-α^F3ex15/Liprin-α^1J-0 mutant peripheral nerves have an accumulation of clear-core vesicles along the axon, compared to wild-type axons. Visualization of synaptic vesicle precursor transport along motor axons in intact third instar larva shows that the flux of cargo in the anterograde direction is significantly decreased in the mutants, while the flux is increased in the retrograde direction. However, the total flux is unchanged. There are no ectopic presynaptic specializations, breakdowns in the plasma membrane, or obvious changes in microtubule density or morphology along motor axons of Liprin-α^F3ex15/Liprin-α^1J-0 mutants.

(Miller et al., 2005)

Liprin-α^1J-0/Liprin-α^R117 larvae have fewer boutons (both type Ib and type Is) at the muscle 6/7 neuromuscular junction than wild type. The branch complexity of the terminal arbor is also decreased compared to wild type.

(Kaufmann et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Liprin-α^1J-0/Liprin-α^1J-0 is a suppressor of abnormal neuroanatomy phenotype of mr³

(van Roessel et al., 2004)

Liprin-α^1J-0/Liprin-alpha[+] is a suppressor of abnormal neuroanatomy phenotype of mr³

(van Roessel et al., 2004)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Liprin-α^1J-0/Liprin-α^R117 has bouton phenotype, non-suppressible by Lar^UAS.cKa/Scer\GAL4^elav.PLu

(Kaufmann et al., 2002)

Suppressor of

Statement

Reference

Liprin-α^1J-0/Liprin-α^1J-0 is a suppressor of NMJ bouton | increased number phenotype of mr³

(van Roessel et al., 2004)

Liprin-α^1J-0/Liprin-alpha[+] is a suppressor of NMJ bouton | increased number phenotype of mr³

(van Roessel et al., 2004)

Liprin-α^1J-0/Liprin-α^1J-0 is a suppressor of embryonic/larval neuromuscular junction phenotype of mr³

(van Roessel et al., 2004)

Liprin-α^1J-0/Liprin-alpha[+] is a suppressor of embryonic/larval neuromuscular junction phenotype of mr³

(van Roessel et al., 2004)

Liprin-α^1J-0/Liprin-α^R117 is a suppressor of bouton | increased number phenotype of Lar^UAS.cKa, Scer\GAL4^elav.PLu

(Kaufmann et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of boutons per synapse seen at the neuromuscular junction of mr³ homozygous third instar larvae is strongly suppressed by Liprin-α^1J-0/+ and is completely suppressed by Liprin-α^1J-0/Liprin-α^1J-0.

(van Roessel et al., 2004)

Liprin-α^1J-0/Liprin-α^R117 larvae expressing Lar^Scer\UAS.cKa under the control of Scer\GAL4^elav.PLu show a reduction in bouton number at the muscle 6/7 neuromuscular junction equivalent to that seen in Liprin-α^1J-0/Liprin-α^R117 single mutants.

(Kaufmann et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

P{lacW} insertion into an intron in the 5' UTR.

(Kaufmann et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Dliprin-α^J1

(van Roessel et al., 2004)

Liprin-α^1J-0

Liprin-α^1J0

(Miller, 2005)

Liprin-α^1J

(Kaufmann et al., 2002)

Liprin-α^J1

(Kaufmann et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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