FlyBase Allele Report: Dmel\Sk2[KG05894]

General Information

Symbol

Dmel\Sk2^KG05894

Species

D. melanogaster

Name

FlyBase ID

FBal0137611

Feature type

allele

Associated gene

Dmel\Sk2

Associated Insertion(s)

P{SUPor-P}Sk2^KG05894

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}Sk2^KG05894

Cytology

Description

P{SUPor-P} insertion in the 5' untranslated region.

(Herr et al., 2004)

Allele components

Component

Use(s)

Inserted element

P{SUPor-P}

insertional mutagenesis tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:3,047,135..3,047,135 [+]

(Yonamine et al., 2011, Herr et al., 2004, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of obesity

CEA

(Villanueva et al., 2019, Walls et al., 2013)

model of cardiomyopathy

CEA

(Walls et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of cardiomyopathy

is ameliorated by lace^GD11234

(Walls et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult fat body

(Walls et al., 2013)

embryonic/larval neuromuscular junction (with Df(3L)BSC671)

(Huang et al., 2018)

lipid droplet | adult stage

(Walls et al., 2013)

terminal bouton | larval stage (with Df(3L)BSC671)

(Huang et al., 2018)

Detailed Description

Statement

Reference

Sk2^KG05894/Df(3L)BSC671 larvae have significantly increased satellite bouton number at the neuromuscular junction of muscle 4, compared to controls, with no effect on bouton size or number.

(Huang et al., 2018)

Sk2^KG05894 homozygous mutant adults present significantly increased survival to starvation, increased body weight, increased adiposity in abdominal sections, and increased sizes of fat body cells and lipid droplets, as compared to controls.

Sk2^KG05894 homozygous mutant adults present increased feeding behavior, as assessed by increased starvation-induced appetite, increased postprandial meal volume, increased average meal frequency and volume, and increased daily food intake, as compared to controls.

(Walls et al., 2013)

Sk2^KG05894 flies appear morphologically normal at room temperature.

(Yonamine et al., 2011)

Mutant animals show a marked increase in sphingolipid content at all stages of the life cycle compared to wild-type animals. Homozygotes show no reduction in viability at any stage of the life cycle and adult longevity is normal. Adults show impaired flight ability (20% reduction in flight performance), although the morphology and patterning of the indirect flight muscles appears normal. Homozygous females produce only 43% of the number of eggs that wild-type females produce. The ovaries do not show morphological defects and no abnormal masses other than an increase in fatty tissue that normally surrounds the gonads. The ovaries are consistently enlarged however, due to an increase in the number of mature eggs.

(Herr et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Sk2^KG05894 has abnormal feeding behavior | adult stage phenotype, enhanceable by ifc⁴/ifc⁴

(Walls et al., 2013)

Sk2^KG05894 has abnormal starvation stress response | adult stage phenotype, enhanceable by ifc⁴/ifc⁴

(Walls et al., 2013)

NOT Enhanced by

Statement

Reference

Sk2^KG05894 has increased cell size | adult stage phenotype, non-enhanceable by ifc⁴/ifc⁴

(Walls et al., 2013)

Enhancer of

Statement

Reference

Sk2^KG05894/Sk2^KG05894 is an enhancer of abnormal starvation stress response | adult stage phenotype of ifc⁴

(Walls et al., 2013)

NOT Enhancer of

Statement

Reference

Sk2^KG05894/Sk2^KG05894 is a non-enhancer of increased cell size | adult stage phenotype of ifc⁴

(Walls et al., 2013)

NOT Suppressor of

Statement

Reference

Sk2^KG05894/Sk2[+] is a non-suppressor of lethal | maternal effect phenotype of Scer\GAL4^Act.PU, wntD^UASp.cEa

(McElwain et al., 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Sk2^KG05894 has adult fat body phenotype, non-enhanceable by ifc⁴/ifc⁴

(Walls et al., 2013)

Sk2^KG05894 has lipid droplet | adult stage phenotype, non-enhanceable by ifc⁴/ifc⁴

(Walls et al., 2013)

NOT Enhancer of

Statement

Reference

Sk2^KG05894/Sk2^KG05894 is a non-enhancer of adult fat body phenotype of ifc⁴

(Walls et al., 2013)

Sk2^KG05894/Sk2^KG05894 is a non-enhancer of lipid droplet | adult stage phenotype of ifc⁴

(Walls et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Sk2^KG05894, ifc⁴ double homozygous adults present a more severe increase in survival in response to starvation as compared to either Sk2^KG05894 or ifc⁴ homozygous mutants.

The increased body weight, increased adiposity in abdominal sections, and increased sizes of fat body cells and lipid droplets observed in either ifc⁴ or Sk2^KG05894 homozygous mutant adults are not exacerbated in ifc⁴, Sk2^KG05894 double homozygous adults.

The increased starvation-induced appetite, but not the increased postprandial meal volume, the increased average meal frequency and volume, or the increased daily food intake, observed in Sk2^KG05894 homozygous mutant adults is exacerbated by ifc⁴ homozygosity.

(Walls et al., 2013)

One copy of Sk2^KG05894 fails to suppress the maternal effect lethality caused by expression of wntD^{Scer\UAS.P\T.cEa} under the control of Scer\GAL4^Act.PU.

(McElwain et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

14133

y¹ w^67c23; P{SUPor-P}Sk2^KG05894 ry⁵⁰⁶

Notes on Origin

Discoverer

Comments

Precise excision of the P{SUPor-P} element fully rescues the impaired flight ability of Sk2^KG05894 flies.

(Herr et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG2159^KG05894

CG32484^KG05894

Sk2^KG05894

(Huang et al., 2018)

Sphk2^KG05894

sk2^KG05894

(McElwain et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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