Allele Dmel\thScer\UAS.cHa
| General Information | |||
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| Symbol | Dmel\thScer\UAS.cHa | Species | D. melanogaster |
| Name | Saccharomyces cerevisiae UAS construct a of Hay | FlyBase ID | FBal0138121 |
| Feature type | allele | Associated gene | Dmel\th |
| Allele class | |||
| Mutagen | in vitro construct - regulatory fusion | ||
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| Description |
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| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
Nature of the Allele
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| Allele class | |||
| Mutagen | |||
| Mutations Mapped to the Genome | |||
Type Location Additional Notes References | |||
| Associated Sequence Data | |||
| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | ||
| UniProtKB/Swiss-Prot | |||
| UniProtKB/TrEMBL | |||
| Progenitor genotype | |||
| Nature of the lesion | Statement Reference | ||
| Carried in construct | (Kanuka et al., 2005, Kanuka et al., 2005, Lohmann et al., 2002, Song et al., 2007, Ditzel et al., 2008, Cullen and McCall, 2004, Choi et al., 2006, Thompson and Cohen, 2006, Janody and Treisman, 2006, Udan et al., 2003, Nolo et al., 2006, Wang et al., 2006, Ribaya et al., 2009, Mathieu et al., 2007, Ganguly et al., 2008, Brun et al., 2006, Kanuka et al., 2005, Florentin and Arama, 2012, Hamaratoglu et al., 2006, Scott et al., 2007, Jang et al., 2008, Lee et al., 2006, Grieder et al., 2009, Wang and Kalderon, 2009, Szuplewski et al., 2009, Mallik and Lakhotia, 2009, Leulier et al., 2006, Bejarano et al., 2010, Sekyrova et al., 2010, Arya et al., 2010, Williams et al., 2006, Rhiner et al., 2010, Malzer et al., 2010, Shen et al., 2009, Hilgers et al., 2010, Tao and Rolls, 2011, Resnik-Docampo and de Celis, 2011, Awasaki et al., 2006, Marchal et al., 2012) | ||
| Cytology | |||
Phenotypic Data
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Phenotypic Class
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Phenotype Manifest In
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Detailed Description
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Statement Reference Pruning of ddaC dendrites is delayed upon expression of th[Scer\UAS.cHa] under the control of Scer\GAL4[ppk.1.9]. The degeneration of dendrites after severing is unaffected however. Expression of thScer\UAS.cHa in Crz-expressing neurons in the ventral nerve cord under the control of Scer\GAL4Crz has no affect on the levels of programmed cell death in these neurons. Expression of thScer\UAS.cHa in the developing eye, driven by Scer\GAL4GMR.PF, results in a slight increase in the number of ommatidial cells. Overexpression of th[Scer\UAS.cHa] under the control of Scer\GAL4[ppk.1.9] suppresses dendritic branch removal. Ectopic macrochaetae form on the scutellum in 60.8% of cases when thScer\UAS.cHa is expressed under the control of Scer\GAL4sca-P309. When thScer\UAS.cHa is driven by Scer\GAL4T80, ectopic branches are seen in the arista. These are of intermediate length along the central core. When thScer\UAS.cHa is driven by Scer\GAL4wg.PM the segment boundary between the embryonic mandibular and maxillary segments is abolished. Also a fusion of the mandibular segment with the procephalic segment is seen. | |||
External Data
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| Linkouts | |||
Interactions
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Phenotypic Class
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Suppressor of | |||
Statement Reference Scer\GAL4bft-Δ263a-G4/thScer\UAS.cHa is a suppressor of visible phenotype of bftunspecified/bftΔ263a-G4 Scer\GAL4C5, Scer\GAL4C5, thScer\UAS.cHa is a suppressor | partially of increased cell death phenotype of Scer\GAL4C5/Scer\GAL4C5, hpoScer\UAS.cUa Scer\GAL4GMR.PF, Scer\GAL4GMR.PF, thScer\UAS.cHa is a suppressor | partially of neuroanatomy defective phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, UbqnScer\UAS.cGa Scer\GAL4GMR.PF, Scer\GAL4GMR.PF, thScer\UAS.cHa is a suppressor of increased cell death phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, lkb1Scer\UAS.cLa Scer\GAL4GMR.PF, Scer\GAL4GMR.PF, thScer\UAS.cHa is a suppressor of increased cell death phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, SlnEP2245, lkb1Scer\UAS.cLa Scer\GAL4GMR.PF, Scer\GAL4GMR.PF, thScer\UAS.cHa is a suppressor of lethal | heat sensitive phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, scnyGS13668 Scer\GAL4GMR.PF, Scer\GAL4GMR.PF, thScer\UAS.cHa is a suppressor of visible | heat sensitive phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, scnyGS13668 Scer\GAL4nub-AC-62/thScer\UAS.cHa is a suppressor of increased cell death | cell autonomous phenotype of Scer\GAL4nub-AC-62, hppyScer\UAS.cMa Scer\GAL4tub.PU/thScer\UAS.cHa is a suppressor of increased cell death | somatic clone phenotype of Vps25N55 thScer\UAS.cHa, Scer\GAL4dpp.blk1 is a suppressor | partially of visible phenotype of HIV-1\VpuScer\UAS.cLa, Scer\GAL4dpp.blk1 thScer\UAS.cHa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is a suppressor of lethal | partially phenotype of Atg1Scer\UAS.cSa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF thScer\UAS.cHa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is a suppressor of visible phenotype of Atg1Scer\UAS.cSa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF | |||
NOT Suppressor of | |||
Statement Reference Scer\GAL4elav-C155, Top1JS, thScer\UAS.cHa is a non-suppressor of bang sensitive | recessive phenotype of eas2 Scer\GAL4nub-AC-62/thScer\UAS.cHa is a non-suppressor of decreased cell size phenotype of Scer\GAL4nub-AC-62, hppyScer\UAS.cMa Scer\GAL4nub-AC-62/thScer\UAS.cHa is a non-suppressor of size defective phenotype of Scer\GAL4nub-AC-62, hppyScer\UAS.cMa Scer\GAL4vg.PM/thScer\UAS.cHa is a non-suppressor of lethal | pupal stage phenotype of Scer\GAL4vg.PM, stwlUY823 thScer\UAS.cHa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is a non-suppressor of visible phenotype of Pink1dsRNA.Scer\UAS.cWa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF | |||
Other | |||
Statement Reference Scer\GAL4tub.PU, Vps25N55, thScer\UAS.cHa has cell growth defective | somatic clone | cell non-autonomous phenotype | |||
Phenotype Manifest In
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Suppressed by | |||
Statement Reference Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, egrScer\UAS.cIa, thScer\UAS.cHa has eye phenotype, suppressible by Hsrωa.Sym.Scer\UAS/HsrωdsRNA.Sym.Scer\UAS, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, rprGMR.PW, thScer\UAS.cHa has eye phenotype, suppressible by Hsrωa.Sym.Scer\UAS/HsrωdsRNA.Sym.Scer\UAS, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF | |||
Suppressor of | |||
Statement Reference Scer\GAL4bft-Δ263a-G4/thScer\UAS.cHa is a suppressor of interommatidial bristle phenotype of bftunspecified/bftΔ263a-G4 Scer\GAL4C5, Scer\GAL4C5, thScer\UAS.cHa is a suppressor | partially of adult head phenotype of Scer\GAL4C5/Scer\GAL4C5, hpoScer\UAS.cUa Scer\GAL4C5, Scer\GAL4C5, thScer\UAS.cHa is a suppressor | partially of wing phenotype of Scer\GAL4C5/Scer\GAL4C5, hpoScer\UAS.cUa Scer\GAL4GMR.PF, Scer\GAL4GMR.PF, thScer\UAS.cHa is a suppressor | partially of eye phenotype of rprGMR.PW Scer\GAL4GMR.PF, Scer\GAL4GMR.PF, thScer\UAS.cHa is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, UbqnScer\UAS.cGa Scer\GAL4GMR.PF, Scer\GAL4GMR.PF, thScer\UAS.cHa is a suppressor of eye | heat sensitive phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, scnyGS13668 Scer\GAL4GMR.PF, Scer\GAL4GMR.PF, thScer\UAS.cHa is a suppressor of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, egrScer\UAS.cIa Scer\GAL4GMR.PF, Scer\GAL4GMR.PF, thScer\UAS.cHa is a suppressor of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, lkb1Scer\UAS.cLa Scer\GAL4αTub84B.PL/thScer\UAS.cHa is a suppressor | partially of wing disc | somatic clone phenotype of cpa107E thScer\UAS.cHa, Scer\GAL4dpp.blk1 is a suppressor | partially of wing phenotype of HIV-1\VpuScer\UAS.cLa, Scer\GAL4dpp.blk1 thScer\UAS.cHa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is a suppressor of eye phenotype of Atg1Scer\UAS.cSa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF thScer\UAS.cHa, Scer\GAL4slbo.2.6 is a suppressor | partially of border follicle cell phenotype of PvrDN.Scer\UAS, Scer\GAL4slbo.2.6, hepAct.Scer\UAS | |||
NOT Suppressor of | |||
Statement Reference Scer\GAL4Act5C.PI, Scer\GAL4Act5C.PI, Scer\GAL4tub.PU, thScer\UAS.cHa is a non-suppressor of follicle stem cell | somatic clone phenotype of CycEWX thScer\UAS.cHa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is a non-suppressor of eye phenotype of Pink1dsRNA.Scer\UAS.cWa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF thScer\UAS.cHa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is a non-suppressor of ommatidium phenotype of Pink1dsRNA.Scer\UAS.cWa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF | |||
Other | |||
Statement Reference CycEScer\UAS.cRa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, banEP3622, thScer\UAS.cHa has cone cell | supernumerary phenotype | |||
Additional Comments
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Genetic Interactions
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Statement Reference Co-expression of th[Scer\UAS.cHa] suppresses the increased apoptosis and increased fraction of mitotic cells phenotypes seen in Scer\GAL4[nub-AC-62], hppy[Scer\UAS.cMa] discs. Expression of th[Scer\UAS.cHa] under the control of Scer\GAL4[ap-md544] completely rescues the wing notching phenotype of mir-9a[J22]/mir-9a[E39] animals. Expression of th[Scer\UAS.cHa] under the control of Scer\GAL4[bft-Δ263a-G4] suppresses the loss of interommatidial bristles which is seen in bft[Δ263a-G4]/bft[unspecified] flies. Expression of th[Scer\UAS.cHa] under the control of Scer\GAL4[tub] in clones in the wing disc does not allow the recovery of large homozygous Df(2L)32FP-5 clones in the notum region. Expression of th[Scer\UAS.cHa] using Scer\GAL4[GMR.PF] rescues the rpr[GMR.PW] small eye phenotype.
Co-expression of th[Scer\UAS.cHa] suppresses the eye phenotype of Scer\GAL4[GMR.PF], egr[Scer\UAS.cIa] flies. Co-expression of th[Scer\UAS.cHa] completely suppresses the lethality caused by expression of scny[GS13668] under the control of Scer\GAL4[GMR.PF] at 25[o]C, and partially suppresses the mutant eye phenotype. Scer\GAL4[tub]- or Scer\GAL4[Act5C.PI]-driven expression of th[Scer\UAS.cHa] does not suppress the loss of follicle stem cells in CycE[WX] mutant clones. Co-expression of thScer\UAS.cHa results in a marked rescue of the degeneration phenotype seen in the eyes of flies expressing UbqnScer\UAS.cGa under the control of Scer\GAL4GMR.PF. Co-expression of th[Scer\UAS.cHa] with both lkb1[Scer\UAS.cLa] and Sln[EP2245] considerably suppresses the apoptotic cell death induced by lkb1[Scer\UAS.cLa] and Sln[EP2245] co-expression in the eye (all under the control of Scer\GAL4[GMR.PF]). The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are partially suppressed by the co-expression of thScer\UAS.cHa. The partial lethality and reduced eye size caused by expression of Atg1[Scer\UAS.cSa] under the control of Scer\GAL4[GMR.PF] is rescued by co-expression of th[Scer\UAS.cHa]. The partial suppression of the bang sensitive phenotype and decreased seizure threshold* of eas[2] homozygotes is itself suppressed by th[Scer\UAS.cHa], Scer\GAL4[elav-C155]. Blocking cell death, through expression of thScer\UAS.cHa in Vps25N55 mutant clones does not affect proliferation or Stat92E activity.
Vps25N55/thScer\UAS.cHa mosaics exhibit non-autonomous cell proliferation. Eye-antennal discs of Vps25N55/thScer\UAS.cHa mosaics are extremely overgrown and can be 5-times as large as wild-type discs. Cell death is completely blocked in Vps25N55/thScer\UAS.cHa mosaics. Vps25N55/thScer\UAS.cHa clones occupy a large fraction of the eye discs, suggesting that these clones have no intrinsic growth disadvantage over wild-type tissue if cell death is blocked. Thus, inhibiting cell death in Vps25N55 clones can give rise to an even stronger overgrowth phenotype. lkb1[Scer\UAS.cLa]-induced apoptosis is completely suppressed by expression of th[Scer\UAS.cHa], both under the control of Scer\GAL4[GMR.PF]. Interestingly, even when lkb1-indueced apoptosis is completely blocked by th, the BrdU and His3 staining patterns do not deviate from the wild-type patterns. Coexpression of thScer\UAS.cHa and CycEScer\UAS.cRa in the developing eye, under the control of Scer\GAL4GMR.PF, results in the generation of many small ommatidial cells.
The extra ommatidial cell phenotype seen in Scer\GAL4GMR.PF>banEP3622 pupal retinae is not enhanced by thScer\UAS.cHa.
Coexpression of banEP3622, CycEScer\UAS.cRa and thScer\UAS.cHa, under the control of Scer\GAL4GMR.PF, causes a large increase in interommatidial, cone and photoreceptor cells. Coexpression of thScer\UAS.cHa and hpoScer\UAS.cUa, under the control of Scer\GAL4ptc-559.1, in wing discs leads to a folding of the epithelium in the ptc expression region.
Expression of thScer\UAS.cHa, under the control of Scer\GAL4tub, in ykiΔ.w mutant clones generated in the wing disc, rescues the survival of these clones but results in clones that are smaller than those generated in wild-type controls.
Clones in the wing disc that express hpoScer\UAS.cUa, under the control of Scer\GAL4tub, (rescued for apoptosis by expression of thScer\UAS.cHa) cause nuclei to move basally, inducing cells to change shape and causing a basally-directed folding of the epithelium. The small head and wing phenotypes, caused by an increase in apoptosis, seen in flies that express hpoScer\UAS.cUa under the control of Scer\GAL4C5 is partially rescued by coexpression of thScer\UAS.cHa. | |||
Xenogenetic Interactions
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Statement Reference Co-expression of th[Scer\UAS.cHa] partially suppresses the wing defects caused by expression of HIV-1\Vpu[Scer\UAS.cLa] under the control of Scer\GAL4[dpp.blk1]. | |||
Complementation & Rescue Data
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| Rescues | |||
| Partially rescues | |||
| Comments | Expression of thScer\UAS.cHa in the salivary glands under the control of Scer\GAL434B rescues programmed cell death in salivary glands expressing thdsRNA.Scer\UAS. The eye phenotype in animals expressing th[dsRNA.Scer\UAS.cLb] under the control of Scer\GAL4[GMR.PF] is fully rescued by co-expression of th[Scer\UAS.cHa]. When thScer\UAS.cHa is driven by Scer\GAL4T80 is th1 animals, a partial rescue of the arista phenotype is seen. Rescued aristae are very short and located only along the posterior of the arista. | ||
Stocks
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| Bloomington | |||
Notes on Origin
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External Crossreferences & Linkouts
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Synonyms & Secondary IDs
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| Reported As | |||
| Symbol Synonym | thScer\UAS.cHa | ||
| Name Synonym | Saccharomyces cerevisiae UAS construct a of Hay | ||
| Secondary FlyBase IDs | |||
References
( 40 ) | |||
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Recent research papers ( 4 ) | |||
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Recent Updates
External Crossreferences & Linkouts