A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Allele Dmel\thScer\UAS.cHa

General Information
SymbolDmel\thScer\UAS.cHaSpeciesD. melanogaster
NameSaccharomyces cerevisiae UAS construct a of HayFlyBase IDFBal0138121
Feature typealleleAssociated geneDmel\th
Allele class
Mutagenin vitro construct - regulatory fusion
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Description
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FB2013_03
FB2013_02
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Allele class
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Statement
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Construct: Scer\UAS sequences drive expression of th.
Carried in construct
Cytology
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Statement
Reference
Pruning of ddaC dendrites is delayed upon expression of th[Scer\UAS.cHa] under the control of Scer\GAL4[ppk.1.9]. The degeneration of dendrites after severing is unaffected however.
Expression of thScer\UAS.cHa in Crz-expressing neurons in the ventral nerve cord under the control of Scer\GAL4Crz has no affect on the levels of programmed cell death in these neurons.
Expression of thScer\UAS.cHa in the developing eye, driven by Scer\GAL4GMR.PF, results in a slight increase in the number of ommatidial cells.
Overexpression of th[Scer\UAS.cHa] under the control of Scer\GAL4[ppk.1.9] suppresses dendritic branch removal.
Ectopic macrochaetae form on the scutellum in 60.8% of cases when thScer\UAS.cHa is expressed under the control of Scer\GAL4sca-P309.
When thScer\UAS.cHa is driven by Scer\GAL4T80, ectopic branches are seen in the arista. These are of intermediate length along the central core.
When thScer\UAS.cHa is driven by Scer\GAL4wg.PM the segment boundary between the embryonic mandibular and maxillary segments is abolished. Also a fusion of the mandibular segment with the procephalic segment is seen.
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Reference
Co-expression of th[Scer\UAS.cHa] suppresses the increased apoptosis and increased fraction of mitotic cells phenotypes seen in Scer\GAL4[nub-AC-62], hppy[Scer\UAS.cMa] discs.
Expression of th[Scer\UAS.cHa] under the control of Scer\GAL4[ap-md544] completely rescues the wing notching phenotype of mir-9a[J22]/mir-9a[E39] animals.
Expression of th[Scer\UAS.cHa] under the control of Scer\GAL4[bft-Δ263a-G4] suppresses the loss of interommatidial bristles which is seen in bft[Δ263a-G4]/bft[unspecified] flies.
Expression of th[Scer\UAS.cHa] under the control of Scer\GAL4[tub] in clones in the wing disc does not allow the recovery of large homozygous Df(2L)32FP-5 clones in the notum region.
Expression of th[Scer\UAS.cHa] using Scer\GAL4[GMR.PF] rescues the rpr[GMR.PW] small eye phenotype. Co-expression of th[Scer\UAS.cHa] suppresses the eye phenotype of Scer\GAL4[GMR.PF], egr[Scer\UAS.cIa] flies.
Co-expression of th[Scer\UAS.cHa] completely suppresses the lethality caused by expression of scny[GS13668] under the control of Scer\GAL4[GMR.PF] at 25[o]C, and partially suppresses the mutant eye phenotype.
Scer\GAL4[tub]- or Scer\GAL4[Act5C.PI]-driven expression of th[Scer\UAS.cHa] does not suppress the loss of follicle stem cells in CycE[WX] mutant clones.
Co-expression of thScer\UAS.cHa results in a marked rescue of the degeneration phenotype seen in the eyes of flies expressing UbqnScer\UAS.cGa under the control of Scer\GAL4GMR.PF.
Co-expression of th[Scer\UAS.cHa] with both lkb1[Scer\UAS.cLa] and Sln[EP2245] considerably suppresses the apoptotic cell death induced by lkb1[Scer\UAS.cLa] and Sln[EP2245] co-expression in the eye (all under the control of Scer\GAL4[GMR.PF]).
The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are partially suppressed by the co-expression of thScer\UAS.cHa.
The partial lethality and reduced eye size caused by expression of Atg1[Scer\UAS.cSa] under the control of Scer\GAL4[GMR.PF] is rescued by co-expression of th[Scer\UAS.cHa].
The partial suppression of the bang sensitive phenotype and decreased seizure threshold* of eas[2] homozygotes is itself suppressed by th[Scer\UAS.cHa], Scer\GAL4[elav-C155].
Blocking cell death, through expression of thScer\UAS.cHa in Vps25N55 mutant clones does not affect proliferation or Stat92E activity. Vps25N55/thScer\UAS.cHa mosaics exhibit non-autonomous cell proliferation. Eye-antennal discs of Vps25N55/thScer\UAS.cHa mosaics are extremely overgrown and can be 5-times as large as wild-type discs. Cell death is completely blocked in Vps25N55/thScer\UAS.cHa mosaics. Vps25N55/thScer\UAS.cHa clones occupy a large fraction of the eye discs, suggesting that these clones have no intrinsic growth disadvantage over wild-type tissue if cell death is blocked. Thus, inhibiting cell death in Vps25N55 clones can give rise to an even stronger overgrowth phenotype.
lkb1[Scer\UAS.cLa]-induced apoptosis is completely suppressed by expression of th[Scer\UAS.cHa], both under the control of Scer\GAL4[GMR.PF]. Interestingly, even when lkb1-indueced apoptosis is completely blocked by th, the BrdU and His3 staining patterns do not deviate from the wild-type patterns.
Coexpression of thScer\UAS.cHa and CycEScer\UAS.cRa in the developing eye, under the control of Scer\GAL4GMR.PF, results in the generation of many small ommatidial cells. The extra ommatidial cell phenotype seen in Scer\GAL4GMR.PF>banEP3622 pupal retinae is not enhanced by thScer\UAS.cHa. Coexpression of banEP3622, CycEScer\UAS.cRa and thScer\UAS.cHa, under the control of Scer\GAL4GMR.PF, causes a large increase in interommatidial, cone and photoreceptor cells.
Coexpression of thScer\UAS.cHa and hpoScer\UAS.cUa, under the control of Scer\GAL4ptc-559.1, in wing discs leads to a folding of the epithelium in the ptc expression region. Expression of thScer\UAS.cHa, under the control of Scer\GAL4tub, in ykiΔ.w mutant clones generated in the wing disc, rescues the survival of these clones but results in clones that are smaller than those generated in wild-type controls. Clones in the wing disc that express hpoScer\UAS.cUa, under the control of Scer\GAL4tub, (rescued for apoptosis by expression of thScer\UAS.cHa) cause nuclei to move basally, inducing cells to change shape and causing a basally-directed folding of the epithelium.
The small head and wing phenotypes, caused by an increase in apoptosis, seen in flies that express hpoScer\UAS.cUa under the control of Scer\GAL4C5 is partially rescued by coexpression of thScer\UAS.cHa.
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Statement
Reference
Co-expression of th[Scer\UAS.cHa] partially suppresses the wing defects caused by expression of HIV-1\Vpu[Scer\UAS.cLa] under the control of Scer\GAL4[dpp.blk1].
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Rescues
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Comments
Expression of thScer\UAS.cHa in the salivary glands under the control of Scer\GAL434B rescues programmed cell death in salivary glands expressing thdsRNA.Scer\UAS.
The eye phenotype in animals expressing th[dsRNA.Scer\UAS.cLb] under the control of Scer\GAL4[GMR.PF] is fully rescued by co-expression of th[Scer\UAS.cHa].
When thScer\UAS.cHa is driven by Scer\GAL4T80 is th1 animals, a partial rescue of the arista phenotype is seen. Rescued aristae are very short and located only along the posterior of the arista.
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Bloomington
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Reported As
Symbol Synonym
thScer\UAS.cHa
 
Name Synonym
Saccharomyces cerevisiae UAS construct a of Hay
Secondary FlyBase IDs
hide References ( 40 )
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hide Recent research papers ( 4 )
Florentin and Arama, 2012, J. Cell Biol. 196(4): 513--527
Caspase levels and execution efficiencies determine the apoptotic potential of the cell. [FBrf0217486]
Marchal et al., 2012, PLoS ONE 7(3): e34310
The HIV-1 Vpu Protein Induces Apoptosis in Drosophila via Activation of JNK Signaling. [FBrf0218002]
Resnik-Docampo and de Celis, 2011, PLoS ONE 6(1): e14528
MAP4K3 Is a Component of the TORC1 Signalling Complex that Modulates Cell Growth and Viability in Drosophila melanogaster. [FBrf0212911]
Tao and Rolls, 2011, J. Neurosci. 31(14): 5398--5405
Dendrites have a rapid program of injury-induced degeneration that is molecularly distinct from developmental pruning. [FBrf0213404]