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General Information
Symbol
Dmel\Diap1UAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Hay
FlyBase ID
FBal0138121
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-DIAP1, UAS-DIAP, UAS- DIAP1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS sequences drive expression of Diap1.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Class IV dendritic arborization neurons expressing Diap1UAS.cHa under the control of Scer\GAL4DcG exhibit a similar proportion of dendrite fragmentation/clearing following dendrite injury, as compared to controls.
Third instar larvae expressing Diap1Scer\UAS.cHa under the control of either Scer\GAL4477 or Scer\GAL4ppk.PG show no defects in the dendritic arborization pattern of class IV dendritic arborizing neurons compared to controls, but the dendritic pruning of these neurons during pupariation is significantly delayed in pupae expressing Diap1Scer\UAS.cHa under Scer\GAL4ppk.PG.
Expression of Diap1Scer\UAS.cHa under the control of Scer\GAL4GMR.PU does not cause significant rough-eye phenotype compared to controls.
Expression of Diap1Scer\UAS.cHa under the control of Scer\GAL4bun-GSG5961 and Scer\GAL4GSG5966 (along with RU486 to induce expression via the GeneSwitch system) does not affect the growth of wild type clones in the adult posterior midgut.
Wing discs from third instar larvae expressing Diap1Scer\UAS.cHa under the control of Scer\GAL469B (using tub-Gal80[ts] to limit the time of expression) are significantly bigger compared to controls.
Compared to controls, Diap1Scer\UAS.cHa Scer\GAL4ple.PF flies exposed to paraquat (to induce oxidative stress) show less degeneration of dopaminergic neurons compared to controls.
Pruning of ddaC dendrites is delayed upon expression of thScer\UAS.cHa under the control of Scer\GAL4ppk.1.9. The degeneration of dendrites after severing is unaffected however.
Expression of thScer\UAS.cHa in Crz-expressing neurons in the ventral nerve cord under the control of Scer\GAL4Crz has no affect on the levels of programmed cell death in these neurons.
Expression of thScer\UAS.cHa in the developing eye, driven by Scer\GAL4GMR.PF, results in a slight increase in the number of ommatidial cells.
Overexpression of thScer\UAS.cHa under the control of Scer\GAL4ppk.1.9 suppresses dendritic branch removal.
Ectopic macrochaetae form on the scutellum in 60.8% of cases when thScer\UAS.cHa is expressed under the control of Scer\GAL4sca-P309.
When thScer\UAS.cHa is driven by Scer\GAL4T80, ectopic branches are seen in the arista. These are of intermediate length along the central core.
When thScer\UAS.cHa is driven by Scer\GAL4wg.PM the segment boundary between the embryonic mandibular and maxillary segments is abolished. Also a fusion of the mandibular segment with the procephalic segment is seen.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Suppressed by
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
The photoreceptor depigmentation phenotype characteristic for adult flies expressing DroncUAS.cSa under the control of Scer\GAL4GMR.PFa is fully suppressed by co-expression of Diap1Scer\UAS.cHa.
The dendritic field coverage defects and loss of terminal branches observed in class IV dendritic arborizing neurons of third instar larvae expressing nosdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4477 is suppressed by co-expression of Diap1Scer\UAS.cHa. The dendritic field coverage and branching defects observed in class IV dendritic arborizing neurons of third instar larvae expressing pumHMS01685 under the control of Scer\GAL4477 is suppressed by co-expression of Diap1Scer\UAS.cHa.
The mild rough-eye phenotype characteristic for flies expressing sraEY07182 under the control of Scer\GAL4GMR.PU is suppressed by co-expression of Diap1Scer\UAS.cHa.
Expression of Diap1Scer\UAS.cHa under the control of Scer\GAL4bun-GSG5961 and Scer\GAL4GSG5966 (along with RU486 to induce expression via the GeneSwitch system) in the gut fully restores growth of wild type clones in the presence of ApcQ8/ApcQ8, Apc2g10/Apc2g10 mutant clones in the posterior midgut, and suppresses the growth of ApcQ8/ApcQ8, Apc2g10/Apc2g10 mutant clones; however, ApcQ8/ApcQ8, Apc2g10/Apc2g10 mutant clones expressing Diap1Scer\UAS.cHa under the control of Scer\GAL4tub.PU do not have suppressed growth.
Inhibition of apoptosis using (Diap1Scer\UAS.cHa under the control of Scer\GAL4αTub84B.PBb) suppresses the contact-dependent delamination seen in wild type 'loser' cells when cell competition is induced in the pupal notum through generation of wild type clones in super-competitive MycαTub84B.PBb-expressing tissue. Inhibiting apoptosis does not suppress the increase in clone splitting (used as a readout for winner-loser cell mixing) or reduction in clone compactness seen in wild type 'loser' clones in the wing disc.
The increased level of cell death (detected by Casp3 antibody staining) observed in wing discs of third instar larvae expressing REGKK102083 RNAi under the control of Scer\GAL4ap.PU is suppressed by co-expression of Diap1Scer\UAS.cHa.
The reduced size of the third larval instar imaginal wing disc characteristic for pcm14 homozygotes is strongly rescued by expression of Diap1Scer\UAS.cHa under the control of Scer\GAL469B (using tub-Gal80[ts] to limit the time of expression) in the mutant background. The resulting disc size is intermediate between that of pcm14 alone and that of Diap1Scer\UAS.cHa-expressing discs (which are bigger than wild-type) and smaller than wild-type.
Co-expression of thScer\UAS.cHa suppresses the eye defects caused by expression of DUBAIGD14040 under the control of Scer\GAL4GMR.PFa.
Expression of thScer\UAS.cHa enhances the eye phenotype seen when Sin3AKK100700 is expressed under the control of Scer\GAL4GMR.PF; approximately a third of all adults examined show enhanced overgrowth and secondary tumors that grow stably at distant sites.
Co-expression of thScer\UAS.cHa almost completely suppresses the reduction in wing size caused by expression of DLPEY09290 under the control of Scer\GAL4Bx-MS1096.
Expression of two copies of thScer\UAS.cHa under the control of Scer\GAL4ey-OK107 fails to suppress the axon degeneration seen in bskflp147E mutant mushroom body neuron clones.
Expression of thScer\UAS.cHa under the control of Scer\GAL4elav.PU does not rescue the climbing defects of Syx17LL06330/Df(3L)Exel8098 flies.
Expression of Diap1Scer\UAS.cHa suppresses the reduction in CCAP/bursicon neuron number seen in newly eclosed flies expressing TBPHKK108354 under the control of Scer\GAL4CCAP.PP. Expression of Diap1Scer\UAS.cHa suppresses the reduction in CCAP/bursicon neuron number seen in newly eclosed flies expressing TBPHScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4CCAP.PP. The wing inflation defects are also rescued.
Co-expression of thScer\UAS.cHa strongly suppresses the small, rough eye phenotype found upon expression of nopoScer\UAS.cUa under the control of Scer\GAL4GMR.PF. Expression of thScer\UAS.cHa suppresses the small eye phenotype in flies expressing egrScer\UAS.cIa under the control of Scer\GAL4GMR.PF.
Co-expression of thScer\UAS.cHa suppresses the increased apoptosis and increased fraction of mitotic cells phenotypes seen in Scer\GAL4nub-AC-62, hppyScer\UAS.cMa discs.
Expression of thScer\UAS.cHa under the control of Scer\GAL4ap-md544 completely rescues the wing notching phenotype of mir-9aJ22/mir-9aE39 animals.
Expression of thScer\UAS.cHa under the control of Scer\GAL4bft-Δ263a-G4 suppresses the loss of interommatidial bristles which is seen in bftΔ263a-G4/bftunspecified flies.
Overexpression of thScer\UAS.cHa under the control of Scer\GAL4ap-md544 suppresses the increased apoptosis in apmd544/+ wing discs. Co-expression of thScer\UAS.cHa partially suppresses the eye-size defect resulting from the ectopic expression of egrScer\UAS.cMa using Scer\GAL4GMR.PF.
The increased apoptosis seen in Scer\GAL4nub-AC-62 RYBPScer\UAS.cBa wing discs is completely inhibited by coexpression of thScer\UAS.cHa. The small wing size seen in Scer\GAL4unspecified RYBPScer\UAS.cBa wings is rescued by coexpression of thScer\UAS.cHa.
Expression of thScer\UAS.cHa under the control of Scer\GAL4tub in clones in the wing disc does not allow the recovery of large homozygous Df(2L)32FP-5 clones in the notum region.
Expression of thScer\UAS.cHa using Scer\GAL4GMR.PF rescues the rprGMR.PW small eye phenotype. Co-expression of thScer\UAS.cHa suppresses the eye phenotype of Scer\GAL4GMR.PF, egrScer\UAS.cIa flies.
Co-expression of thScer\UAS.cHa completely suppresses the lethality caused by expression of scnyGS13668 under the control of Scer\GAL4GMR.PF at 25[o]C, and partially suppresses the mutant eye phenotype.
Scer\GAL4tub- or Scer\GAL4Act5C.PI-driven expression of thScer\UAS.cHa does not suppress the loss of follicle stem cells in CycEWX mutant clones.
Co-expression of thScer\UAS.cHa results in a marked rescue of the degeneration phenotype seen in the eyes of flies expressing UbqnScer\UAS.cGa under the control of Scer\GAL4GMR.PF.
Co-expression of thScer\UAS.cHa suppresses the defective eye phenotype caused by expression of Sir2Scer\UAS.cGa under the control of Scer\GAL4GMR.PU.
Co-expression of thScer\UAS.cHa with both lkb1Scer\UAS.cLa and SlnEP2245 considerably suppresses the apoptotic cell death induced by lkb1Scer\UAS.cLa and SlnEP2245 co-expression in the eye (all under the control of Scer\GAL4GMR.PF).
The border follicle cell migration defects that are seen in flies co-expressing both PvrDN.Scer\UAS and hepAct.Scer\UAS under the control of Scer\GAL4slbo.2.6 are partially suppressed by the co-expression of thScer\UAS.cHa.
The partial lethality and reduced eye size caused by expression of Atg1Scer\UAS.cSa under the control of Scer\GAL4GMR.PF is rescued by co-expression of thScer\UAS.cHa.
The partial suppression of the bang sensitive phenotype and decreased seizure threshold* of eas2 homozygotes is itself suppressed by thScer\UAS.cHa, Scer\GAL4elav-C155.
Blocking cell death, through expression of thScer\UAS.cHa in Vps25N55 mutant clones does not affect proliferation or Stat92E activity. Vps25N55/thScer\UAS.cHa mosaics exhibit non-autonomous cell proliferation. Eye-antennal discs of Vps25N55/thScer\UAS.cHa mosaics are extremely overgrown and can be 5-times as large as wild-type discs. Cell death is completely blocked in Vps25N55/thScer\UAS.cHa mosaics. Vps25N55/thScer\UAS.cHa clones occupy a large fraction of the eye discs, suggesting that these clones have no intrinsic growth disadvantage over wild-type tissue if cell death is blocked. Thus, inhibiting cell death in Vps25N55 clones can give rise to an even stronger overgrowth phenotype.
lkb1Scer\UAS.cLa-induced apoptosis is completely suppressed by expression of thScer\UAS.cHa, both under the control of Scer\GAL4GMR.PF. Interestingly, even when lkb1-indueced apoptosis is completely blocked by th, the BrdU and His3 staining patterns do not deviate from the wild-type patterns.
Coexpression of thScer\UAS.cHa and CycEScer\UAS.cRa in the developing eye, under the control of Scer\GAL4GMR.PF, results in the generation of many small ommatidial cells. The extra ommatidial cell phenotype seen in Scer\GAL4GMR.PF>banEP3622 pupal retinae is not enhanced by thScer\UAS.cHa. Coexpression of banEP3622, CycEScer\UAS.cRa and thScer\UAS.cHa, under the control of Scer\GAL4GMR.PF, causes a large increase in interommatidial, cone and photoreceptor cells.
Coexpression of thScer\UAS.cHa and hpoScer\UAS.cUa, under the control of Scer\GAL4ptc-559.1, in wing discs leads to a folding of the epithelium in the ptc expression region. Expression of thScer\UAS.cHa, under the control of Scer\GAL4tub, in ykiΔ.w mutant clones generated in the wing disc, rescues the survival of these clones but results in clones that are smaller than those generated in wild-type controls. Clones in the wing disc that express hpoScer\UAS.cUa, under the control of Scer\GAL4tub, (rescued for apoptosis by expression of thScer\UAS.cHa) cause nuclei to move basally, inducing cells to change shape and causing a basally-directed folding of the epithelium.
The small head and wing phenotypes, caused by an increase in apoptosis, seen in flies that express hpoScer\UAS.cUa under the control of Scer\GAL4C5 is partially rescued by coexpression of thScer\UAS.cHa.
Xenogenetic Interactions
Statement
Reference
The eye degeneration and the associated rough eye appearance, ommatidial fusion and pigmentation loss characteristic for adult flies expressing Hsap\FUSScer\UAS.cIa under the control of Scer\GAL4GMR.PS cannot be rescued by co-expression of Diap1Scer\UAS.cHa.
Co-expression of thScer\UAS.cHa partially suppresses the wing defects caused by expression of HIV-1\VpuScer\UAS.cLa under the control of Scer\GAL4dpp.blk1.
Complementation and Rescue Data
Partially rescues
Comments
Expression of thScer\UAS.cHa in the salivary glands under the control of Scer\GAL434B rescues programmed cell death in salivary glands expressing thdsRNA.Scer\UAS.
The eye phenotype in animals expressing thdsRNA.Scer\UAS.cLb under the control of Scer\GAL4GMR.PF is fully rescued by co-expression of thScer\UAS.cHa.
When thScer\UAS.cHa is driven by Scer\GAL4T80 is th1 animals, a partial rescue of the arista phenotype is seen. Rescued aristae are very short and located only along the posterior of the arista.
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Diap1Scer\UAS.cHa
Diap1UAS.cHa
thScer\UAS.cHa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Hay
Secondary FlyBase IDs
    References (76)