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General Information
Symbol
Dmel\sav3
Species
D. melanogaster
Name
FlyBase ID
FBal0138135
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Contains a frameshift mutation and generates a protein consisting of 406 sav encoded amino acids and a C terminal portion of 84 amino acids derived from the use of an alternative open reading frame.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    sav3 homozygous mutant embryos display a significant increase in the frequency of hemisegments with abnormal number of neurons in the asymmetrically dividing RP2 neural lineage.

    sav3 homozygous embryos from mothers whose germline consisted entirely of sav3 mutant cells (generated by the Ovo[D] germline clone method, which thus are both zygotically and maternally mutant for sav3, display defects in the asymmetric neuroblast (NB) division: disturbed asymmetric localization of polarity and cell-fate determinants as well as high frequency of mitotic spindle orientation defects.

    Cells in homozygous clones in the wing disc accumulate F-actin near the apical surface.

    Homozygous clones in the eye show progressive degeneration of photoreceptor cells. Autophagic vesicles with partially degraded content accumulate in the mutant photoreceptor cells compared to wild-type cells.

    sav3 mutant adult Malpighian tubule clones are significantly enlarged compared to wild-type clones. The mitotic index is increased compared to controls. Mutant clusters typically contain proportionally more renal and nephric stem cells (RNSCs) and renablasts (RBs) compared to control clones. Disrupted cell polarity is observed in mutant cells.

    Animals containing mutant eye discs generated using the eyFLP-cell lethal system eclose, and have overgrown eyes.

    Mutant eye discs (generated using the eyFLP-cell lethal system) do not show defects in epithelial organisation or neuronal differentiation.

    Heterozygous larvae show no significant defects in dendrite morphology of ddaC neurons.

    Apoptosis is reduced by up to 3-fold in sav3 clones of wing or eye discs in response to γ-rays compared to wild-type tissue.

    sav3 mutant ddaC MARCM clones exhibit dendritic defects, including highly penetrant simplifcation of dendritic trees. In severely affected clones (one fifth), most of the high-order branches are missing, whereas moderately affected clones (approximately 75% of the clones) exhibit a partial loss of their fine branches and major branches.

    sav3 heterozygotes do not exhibit an obvious dendritic phenotype.

    In sav3 mutants, ddaC dendrites are indistinguishable from wild-type controls at 24-28 hours after egg-laying. By 48-52 hours after egg laying, sav3 mutant dendrites tile the body wall as in wild-type. During the following 24 hours, however, sav3 dendrites disappear from the body wall, implying sav is required for maintenance of the already established tiling of dendrites.

    When somatic clones of sav3 homozygous cells are generated throughout the eye disc using Scer\FLP1ey.PN, nearly all of the resulting animals survive to eclosion.

    Mosaic flies with eyes containing clones homozygous for sav3 (clones generated using the "eyFLP" system) have large eyes, with an increased representation of mutant tissue over wild-type tissue. Clones made in other parts of the fly including the notum and haltere also display outgrowths. In mutant clones in the adult retina, half of all ommatidia lack one or more photoreceptor cells. However there is increased spacing between adjacent ommatidia - there are many additional interommatidial cells. When mutant clones are made in eye imaginal discs, mutant cells continue to proliferate for 12-24 hours after wild-type cells stop dividing, but are eventually able to exit from the cell cycle and undergo terminal differentiation. When examined by flow cytometry, the cycling cells in the anterior portion of the eye disc have a distribution very similar to that of wild-type cells. The mutant cells are slightly smaller than their wild-type counterparts. Posterior to the morphogenetic furrow, mutant cells have an increased proportion of cells in S2 and G2, indicating that the cells continue to cycle.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhancer of
    Statement
    Reference

    sav[+]/sav3 is an enhancer of partially lethal - majority die phenotype of hpo+t4.0, hpo42-20

    NOT Enhancer of
    Statement
    Reference

    sav3 is a non-enhancer of neuroanatomy defective phenotype of wtsx1

    Suppressor of
    NOT Suppressor of
    Statement
    Reference
    Other
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference
    Enhancer of
    Statement
    Reference
    NOT Enhancer of
    Statement
    Reference

    sav3 is a non-enhancer of dorsal multidendritic neuron ddaC | somatic clone & dendritic tree phenotype of wtsx1

    NOT Suppressor of
    Statement
    Reference
    Other
    Statement
    Reference

    Pc3, sav[+]/sav3 has dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype

    sav3, wtsx1/wts[+] has dorsal multidendritic neuron ddaC | somatic clone & dendritic tree phenotype

    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The proportion of hemisegments with abnormal number of neurons in the asymmetrically dividing RP2 neural lineage is significantly increased in cno2/+;sav3/+ double heterozygous embryos compared to either of the single heterozygotes or wild type.

    The rough eye phenotype in adult flies induced by expression of MagiScer\UAS.T:Avic\GFP under the control of Scer\GAL4GMR.PU is slightly worsened by combination with single copy of sav3/+.

    Mer4/sav3 double mutant eyes exhibit a massive increase in the number of supernumerary interommatidial cells, compared to either single mutant.

    Ras85DΔC40B, sav3 double mutant adult Malpighian tubule clones display only a few differentiated renalcytes (RCs), like the single Ras85DΔC40B mutant phenotype.

    Pc3/sav3 double heterozygous larvae show dendritic defects in ddaC neurons; there is a significant reduction in the number of dendritic branchpoints.

    sav3 allows recovery of Df(1)su(s)R194/+ clones in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals). sav3 Df(1)su(s)R194/+ cells are present in the wing disc 72 hours after induction and show significantly reduced numbers of dying cells compared to Df(1)su(s)R194/+ clones.

    The p53GMR.Ex-mediated induction of apoptosis in the posterior portion of late larval eye discs is reduced in sav3 clones.

    sav3/wtsx1 transheterozygotes exhibit simplified dendrites similar to moderately affected sav3 MARCM clones. These double mutants show a severe dendrite effect comparable to wtsx1 MARCM clones.

    Scer\GAL4GMR.PF-driven hpoScer\UAS.cPa expression-induced apoptosis is largely unimpaired in sav3 mutant clones.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments

    Expression of savScer\UAS.cEa, under the control of Scer\GAL4ppk.PG largely rescues the dendritic phenotype of sav3.

    sav3 fails to complement the lethality of Df(3R)hh.

    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    References (26)