FlyBase Allele Report: Dmel\p53[5A-1-4]

Under normal conditions p53^5A-1-4 adults do not exhibit an increased cell death at stage 7-10 egg chambers or in germarium compared to controls. 6 hours after 3 Gy radiation they do not exhibit an increased cell death in germarium, different than controls. After 40 Gy radiation, they exhibit a decreased and delayed cell death in germarium while they exhibit normal cell death at stage 7-10 egg chambers compared to controls. Under starvation conditions they exhibit increased cell death in germarium and stage 7-10 egg chambers, similar to controls.

Under normal conditions p53^5A-1-4 females, p53^5A-1-4 females expressing p53^UASp.A (one or two copies) under the control of Scer\GAL4^nos.PG or p53^5A-1-4 females expressing p53^UASp.B under the control of Scer\GAL4^VP16.nos.UTR do not exhibit any changes in the number of germline stem cells compared to control adults. After 40 Gy irradiation, p53^5A-1-4 females exhibit a continuous decrease (by day 6) in the number of germline stem cells compared to untreated adults.

Under normal conditions p53^5A-1-4 females' daily egg production is similar to controls. 3 days after 40 Gy but not 3 Gy irradiation, they exhibit a reduction in the daily egg production compared to untreated adults as in the case of controls, however they never recover from it different than controls.

Expression of p53^UASp.A under the control of Scer\GAL4^VP16.nos.UTR in p53^5A-1-4 background leads to severe defects in the daily egg production and severe morphological defects in germline cells in females when compared to control adults.

Under normal conditions, p53^5A-1-4 female adults do not exhibit any changes in fertility (number of pupa produced per day) compared to controls. After 40 Gy irradiation, they exhibit decreased fertility at 0-4 days similar to controls however they do not exhibit an increase/recovery in fertility at 4-15 days different than controls.

Under normal conditions p53^5A-1-4 females expressing two copies of p53^UASp.A under the control of Scer\GAL4^nos.PG exhibit severely decreased fertility compared to control adults.

(Park et al., 2019)

p53^5A-1-4 adults are show a severely increased sensitivity to paraquat-induced oxidative stress, as compared to controls. In agreement, whole body homogenates show higher levels of apoptosis markers (cleaved Dcp1 and cleaved Casp3) than controls only upon paraquat treatment. However, the adult brain of p53^5A-1-4 mutants show increased levels of apoptosis (cleaved casp3) under both paraquat treatment and basal conditions, although higher in the paraquat treatment condition.

(Robin et al., 2019)

p53^5A-1-4 homozygotes exhibit a severe increase in the proportion of hyperplastic testes as compared to controls; the apical tip of these testes exhibit a significant decrease in necrosis (shown by the number of spermatogonial cysts bearing Propidium Iodine-positive or TUNEL-positive cells), but no changes in the frequency of mitotic cells, as compared to controls.

(Napoletano et al., 2017)

p53^5A-1-4 homozygous adults have increased mortality rate following oral infection with Drosophila C virus; they also have significantly increased levels of virus (qRT-PCR) at 3 days post-infection.

(Wang et al., 2017)

p53^5A-1-4 suppresses the eye ablation phenotype resulting from an irreparable DNA double-stranded break in the BARTL (Bar and Telomere Loss) assay, such that the flies have wild-type eyes.

(Chakraborty et al., 2015)

Within 4 h after irradiation, the imaginal discs of homozygous p53^5A-1-4 larvae have very few apoptotic cells compared with wild-type.

(Zhang et al., 2015)

p53^5A-1-4 mutant flies exhibit a reduction in survival following acute starvation during early adulthood (1-2 days).

(Barrio et al., 2014)

p53^5A-1-4/p53^5A-1-4 flies do not show any visible wing defects compared to wild type controls.

(Simón et al., 2014)

p53^5A-1-4 mutant wing disc clones grow to a much larger size than control cell clones.

p53^5A-1-4 mutant wing disc cells or fat body cells exhibit disrupted mitochondrial morphology, with a variety of shapes including globules and donut shapes as well as long filaments, as seen in wild-type.

(de la Cova et al., 2014)

Expression of p53^{Scer\UAS.Dp53} under the control of Scer\GAL4^Bx-MS1096 induces apoptosis in wing discs. A small increase in cell proliferation is seen compared to controls, but less than is seen when p53^{Scer\UAS.DΔNp53} is expressed. An increase in apoptosis is also seen when p53^{Scer\UAS.Dp53} is expressed in a p53^5A-1-4 mutant background.

Expression of p53^{Scer\UAS.DΔNp53} under the control of Scer\GAL4^Bx-MS1096 induces apoptosis in wing discs. Increased cell proliferation is seen compared to controls. An increase in apoptosis is also seen when p53^{Scer\UAS.Dp53} is expressed in a p53^5A-1-4 mutant background.

(Dichtel-Danjoy et al., 2013)

Mitotic chromosomes from the brains of p53^5A-1-4/p53^5A-1-4 third instar larvae treated with radiation show significant increases in the number of breaks per nucleus compared to wild type (radiation sensitive). Wild type larval wing discs show two phases of cell death in response to irradiation: first signs of cell death are clear after 4h and plateau around 8h, with cell death again increasing from 16-24h; irradiated p53^5A-1-4/p53^5A-1-4 discs do not show early induction of cell death but do show an elevation between 16-24h.

(Wang et al., 2013)

Homozygous wing discs show little, if any, apoptosis 4 hours after exposure to 4000 rads of X-ray irradiation, in contrast to the high level of apoptosis seen in wild-type discs. At 20 hours after irradiation, apoptosis levels in the mutant discs are comparable to those seen in wild-type discs.

(Gowda et al., 2012)

Mutant larvae show greater sensitivity to 0.5 or 1μM maytansinol than wild-type controls.

(Edwards et al., 2011)

In contrast to wild-type controls, virtually no apoptotic cells are observed in homozygous p53^5A-1-4 mutant eye discs after treatment with ionising irradiation.

(Moon et al., 2008)

The presence of p53^5A-1-4 largely abolishes the apoptotic response seen in cells containing dicentric chromosomes (generated using Scer\FLP1^hs.PP-induced recombination in chromosomes containing inverted Scer\FRT sites). At 12-14 hours after heat shock eye disc apoptosis is suppressed when dicentrics are generated using any of DcY-FrTr4B1A, DcYy⁺, P{FRT(w^hs)}(8F)105, P{inv.FRT}Dc2-127, P{inv.FRT}Dc2-FrTr1B or P{inv.FRT}Dc3-FrTr1D. Wing disc apoptosis is also suppressed 12-14 hours after heat shock, and this can be seen using any of DcY-FrTr4B1A, DcYy⁺, DcY-H1, DcY-H2, DcY-H3, P{FRT(w^hs)}(8F)105, P{inv.FRT}Dc2-127, P{inv.FRT}Dc2-FrTr1B or P{inv.FRT}Dc3-FrTr1D. However the wing disc apoptosis seen 24 hours after dicentric formation is only suppressed in Y chromosome dicentrics; apoptosis is still seen when dicentric chromosomes are induced on the X or an autosome. Some cell death is still observed when dicentrics are generated using DcYy⁺. When dicentrics are generated in larval neuroblast cells, fewer than 40% of p53^5A-1-4 mutant larval neuroblast cells have normal karyotypes 96 hours after heat shock of P{inv.FRT}Dc2-FrTr1B or P{FRT(w^hs)}(8F)105, whereas 80-90% appear normal in the dicentric controls. Phenotypes observed in cells with abnormal karyotypes include the presence of multiple acentric chromatids, chromosome fusions and tetraploidy.

(Titen and Golic, 2008)

Programmed cell death of primordial germ cells (PGCs), which is essentially complete by stage 12 of embryogenesis in wild type, is disrupted in mutant embryos; the total average number of PGCs is normal in the mutants at stage 10, but although there is a gradual reduction in the number of PGCs, it is decreased compared to wild type, such that at stage 14 the average number of PGCs is higher in the mutant embryos compared to wild type.

The average number of PGCs per embryo in p53^5A-1-4/Dp(3;3)p53^-ns mutants is similar to wild type at stage 10, but by stage 14 the average number of PGCs in the mutant embryo is significantly higher than in wild type, as programmed cell is defective in the mutant embryos.

Although PGCs are able to incorporate into the gonad in p53^5A-1-4 embryos, an average of 9.1 germ cells ectopic to the gonad are seen at stage 14 (compared to the wild-type average of 0.4 ectopic germ cells at this stage).

Although PGCs are able to incorporate into the gonad in p53^5A-1-4/Dp(3;3)p53^-ns embryos, an average of 6.5 germ cells ectopic to the gonad are seen at stage 14 (compared to the wild-type average of 0.4 ectopic germ cells at this stage).

(Yamada et al., 2008)

Mutant third instar larvae show a dramatic reduction in survival after irradiation with 2krad X rays compared to control larvae.

(Bodai et al., 2007)

p53^5A-1-4 mutants display a reduction in radiation-induced cell death, with only approximately 50% eclosing after irradiation with 4,000R.

(Wichmann et al., 2006)

p53^5A-1-4 flies do not show age-dependent degeneration of rhabdomeres or photoreceptor cells.

(Bae et al., 2005)

Homozygous mutant animals have compromised viability, with 83.3% of third instar larvae eclosing to adults (compared to 98.1% in wild-type). These mutants show a consistent, fully penetrant lack of cell death at times it is readily detectable in other backgrounds. When irradiated, 71% of animals eclose into adults.

(Jaklevic and Su, 2004)

No apoptosis is observed in response to irradiation in mutant third instar wing disc (in contrast to wild-type discs, which show an increase in apoptosis after irradiation).

(Qi et al., 2004)

Shows no obvious phenotype.

(Golic, 2002.10.25)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

p53^5A-1-4 has visible | maternal effect phenotype, enhanceable by okr^AA/okr^RU

(Chakravarti et al., 2022)

NOT Enhanced by

Statement

Reference

p53^5A-1-4 has radiation sensitive | third instar larval stage phenotype, non-enhanceable by Clbn^1Q/Clbn^1Q

(Wang et al., 2013)

p53^5A-1-4 has decreased cell death phenotype, non-enhanceable by lok^p6

(Wichmann et al., 2006)

Suppressed by

Statement

Reference

Myc^αTub84B.PBb, p53^5A-1-4 has increased cell growth rate | somatic clone phenotype, suppressible by BacA\p35^GMR.PH

(de la Cova et al., 2014)

NOT suppressed by

Statement

Reference

p53^5A-1-4 has increased cell size | somatic clone phenotype, non-suppressible by BacA\p35^GMR.PH

(de la Cova et al., 2014)

p53^5A-1-4 has radiation sensitive | third instar larval stage phenotype, non-suppressible by Clbn^1Q/Clbn^1Q

(Wang et al., 2013)

Enhancer of

Statement

Reference

p53^5A-1-4/p53^5A-1-4 is an enhancer of decreased cell number | adult stage | female | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

p53^5A-1-4 is an enhancer of increased cell death phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Merlo et al., 2014)

p53^5A-1-4 is an enhancer of abnormal mitotic cell cycle phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Merlo et al., 2014)

p53^5A-1-4/p53[+] is an enhancer of visible | adult stage | dominant phenotype of N^55e11

(Simón et al., 2014)

p53^5A-1-4/p53[+] is an enhancer of visible | adult stage | dominant phenotype of wg¹

(Simón et al., 2014)

p53^5A-1-4/p53^5A-1-4 is an enhancer of melanotic mass phenotype | third instar larval stage phenotype of tefu^stg

(Wang et al., 2013)

NOT Enhancer of

Statement

Reference

p53^5A-1-4/p53^5A-1-4 is a non-enhancer of short lived | RU486 conditional phenotype of Hsap\HTT^{N548.Q128.UAS}, Scer\GAL4^{repo.Switch.2301}

(Martin et al., 2021)

p53^5A-1-4/Dp(3;3)M95A⁺13 is a non-enhancer of visible | adult stage | dominant phenotype of N^55e11

(Simón et al., 2014)

p53^5A-1-4/p53^5A-1-4 is a non-enhancer of radiation sensitive | larval stage phenotype of Clbn^1Q

(Wang et al., 2013)

p53^5A-1-4 is a non-enhancer of increased cell death phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PF

(Moon et al., 2008)

p53^5A-1-4 is a non-enhancer of increased cell death phenotype of Rbf^120a

(Moon et al., 2008)

p53^5A-1-4 is a non-enhancer of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Suppressor of

Statement

Reference

p53^5A-1-4 is a suppressor | partially of decreased cell number | oogenesis phenotype of Non1^HMS05872, Scer\GAL4^nanos.PG

(Martin et al., 2022)

p53^5A-1-4 is a suppressor | partially of decreased cell number | oogenesis phenotype of Scer\GAL4^nanos.PG, pths^GL00549

(Martin et al., 2022)

p53^5A-1-4 is a suppressor | partially of decreased cell number | oogenesis phenotype of Scer\GAL4^nanos.PG, ais^HMS00325

(Martin et al., 2022)

nej^GD8975, p53^5A-1-4, p53^5A-1-4, Scer\GAL4^{repo.Switch.2301} is a suppressor | partially of short lived | RU486 conditional phenotype of Hsap\HTT^{N548.Q128.UAS}, Scer\GAL4^{repo.Switch.2301}

(Martin et al., 2021)

p53^5A-1-4/nej^GD8975, Scer\GAL4^{repo.Switch.2301} is a suppressor | partially of short lived | RU486 conditional phenotype of Hsap\HTT^{N548.Q128.UAS}, Scer\GAL4^{repo.Switch.2301}

(Martin et al., 2021)

p53^5A-1-4/p53[+] is a suppressor of decreased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

p53^5A-1-4 is a suppressor of visible phenotype of hid^GMR.PG

(Chakraborty et al., 2015)

p53^5A-1-4/p53^11-1B-1 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Fen1^EY12786

(Kuang et al., 2014)

p53^5A-1-4 is a suppressor of increased cell growth rate | somatic clone phenotype of Myc^αTub84B.PBb

(de la Cova et al., 2014)

p53^5A-1-4/p53^5A-1-4 is a suppressor of visible phenotype of Fcp1^RNAi.UAS.WIZ, Scer\GAL4^vg.PU

(Schauer et al., 2009)

p53^5A-1-4 is a suppressor | partially of increased cell death phenotype of dup^{hsp70.Tag:MYC}

(Mehrotra et al., 2008)

p53^5A-1-4 is a suppressor of increased cell death phenotype of Rbf^120a

(Moon et al., 2008)

p53^5A-1-4/p53^5A-1-4 is a suppressor of visible phenotype of E(mus304)[+]/PolA1^Emus304, mei-41^29D

(LaRocque et al., 2007)

p53^5A-1-4/p53^5A-1-4 is a suppressor of increased cell death phenotype of E(mus304)[+]/PolA1^Emus304, mei-41^29D

(LaRocque et al., 2007)

p53^5A-1-4/p53^5A-1-4 is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\HTT^GMR.Q120

(Bae et al., 2005)

NOT Suppressor of

Statement

Reference

p53^5A-1-4 is a non-suppressor of female sterile phenotype of okr^AA/okr^RU

(Chakravarti et al., 2022)

p53^5A-1-4 is a non-suppressor of lethal - all die during embryonic stage | maternal effect phenotype of okr^AA/okr^RU

(Chakravarti et al., 2022)

p53^5A-1-4/p53^5A-1-4 is a non-suppressor of short lived | RU486 conditional phenotype of Hsap\HTT^{N548.Q128.UAS}, Scer\GAL4^{repo.Switch.2301}

(Martin et al., 2021)

p53^5A-1-4/p53^5A-1-4 is a non-suppressor of increased cell death | third instar larval stage phenotype of Scer\GAL4^ap-md544, pnut^GD1512

(Gerlach et al., 2018)

p53^5A-1-4/p53^11-1B-1 is a non-suppressor of increased cell death | somatic clone phenotype of M(2)56F^H

(Kale et al., 2015)

p53^5A-1-4 is a non-suppressor of FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype of Df(1)su(s)R194, RpL36^+t4

(Kale et al., 2015)

p53^5A-1-4/p53^5A-1-4 is a non-suppressor of increased cell death | third instar larval stage phenotype of Ipk2^20B

(Seeds et al., 2015)

p53^5A-1-4/p53^5A-1-4 is a non-suppressor of radiation sensitive | larval stage phenotype of Clbn^1Q

(Wang et al., 2013)

p53^5A-1-4/p53[+] is a non-suppressor of melanotic necrosis | pupal stage P5 phenotype of Pfas^1-6

(Holland et al., 2011)

p53^5A-1-4/p53^5A-1-4 is a non-suppressor of abnormal size | third instar larval stage phenotype of Scer\GAL4^ey.PU, vito^GD10890

(Marinho et al., 2011)

p53^5A-1-4/p53^5A-1-4 is a non-suppressor of increased cell death phenotype of Scer\GAL4^ey.PU, vito^GD10890

(Marinho et al., 2011)

p53^5A-1-4/p53^11-1B-1 is a non-suppressor of increased cell death phenotype of Myc^UAS.cZa, Scer\GAL4^ap-md544

(Montero et al., 2008)

p53^5A-1-4 is a non-suppressor of increased cell death phenotype of Rbf^120a

(Moon et al., 2008)

p53^5A-1-4 is a non-suppressor of increased cell death phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PF

(Moon et al., 2008)

p53^5A-1-4 is a non-suppressor of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Other

Statement

Reference

okr^AA/okr^RU, p53^5A-1-4 has abnormal DNA repair | oogenesis phenotype

(Chakravarti et al., 2022)

Clbn^1Q, p53^5A-1-4 has decreased cell death | third instar larval stage | conditional phenotype

(Wang et al., 2013)

Ada2b², p53^5A-1-4 has abnormal cell death | conditional phenotype

(Qi et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

p53^5A-1-4 has egg | maternal effect phenotype, enhanceable by okr^AA/okr^RU

(Chakravarti et al., 2022)

NOT suppressed by

Statement

Reference

p53^5A-1-4 has wing disc | somatic clone phenotype, non-suppressible by BacA\p35^GMR.PH

(de la Cova et al., 2014)

Enhancer of

Statement

Reference

p53^5A-1-4/p53^5A-1-4 is an enhancer of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

p53^5A-1-4 is an enhancer of brain phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Merlo et al., 2014)

p53^5A-1-4/p53[+] is an enhancer of wing phenotype of N^55e11

(Simón et al., 2014)

p53^5A-1-4/p53[+] is an enhancer of wing phenotype of wg¹

(Simón et al., 2014)

p53^5A-1-4/p53^5A-1-4 is an enhancer of melanotic mass | third instar larval stage phenotype of tefu^stg

(Wang et al., 2013)

NOT Enhancer of

Statement

Reference

p53^5A-1-4/Dp(3;3)M95A⁺13 is a non-enhancer of wing phenotype of N^55e11

(Simón et al., 2014)

p53^5A-1-4 is a non-enhancer of eye disc posterior to the morphogenetic furrow phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PF

(Moon et al., 2008)

p53^5A-1-4 is a non-enhancer of eye disc phenotype of Rbf^120a

(Moon et al., 2008)

p53^5A-1-4 is a non-enhancer of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Suppressor of

Statement

Reference

p53^5A-1-4 is a suppressor | partially of karyosome phenotype of okr^AA/okr^RU

(Chakravarti et al., 2022)

p53^5A-1-4 is a suppressor | partially of female germline cyst phenotype of Non1^HMS05872, Scer\GAL4^nanos.PG

(Martin et al., 2022)

p53^5A-1-4 is a suppressor | partially of female germline cell | decreased number phenotype of Non1^HMS05872, Scer\GAL4^nanos.PG

(Martin et al., 2022)

p53^5A-1-4 is a suppressor | partially of germarium phenotype of Non1^HMS05872, Scer\GAL4^nanos.PG

(Martin et al., 2022)

p53^5A-1-4 is a suppressor | partially of germarium phenotype of Scer\GAL4^nanos.PG, ath^HMC04417

(Martin et al., 2022)

p53^5A-1-4 is a suppressor | partially of female germline cyst phenotype of Scer\GAL4^nanos.PG, ath^HMC04417

(Martin et al., 2022)

p53^5A-1-4 is a suppressor | partially of female germline cell | decreased number phenotype of Scer\GAL4^nanos.PG, pths^GL00549

(Martin et al., 2022)

p53^5A-1-4 is a suppressor | partially of female germline cyst phenotype of Scer\GAL4^nanos.PG, pths^GL00549

(Martin et al., 2022)

p53^5A-1-4 is a suppressor | partially of germarium phenotype of Scer\GAL4^nanos.PG, pths^GL00549

(Martin et al., 2022)

p53^5A-1-4 is a suppressor | partially of germarium phenotype of Scer\GAL4^nanos.PG, ais^HMS00325

(Martin et al., 2022)

p53^5A-1-4 is a suppressor | partially of female germline cyst phenotype of Scer\GAL4^nanos.PG, ais^HMS00325

(Martin et al., 2022)

p53^5A-1-4 is a suppressor | partially of female germline cell | decreased number phenotype of Scer\GAL4^nanos.PG, ais^HMS00325

(Martin et al., 2022)

p53^5A-1-4/p53[+] is a suppressor of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreI^hs.PR

(Ma et al., 2016)

p53^5A-1-4 is a suppressor of eye phenotype of hid^GMR.PG

(Chakraborty et al., 2015)

p53^5A-1-4/p53^5A-1-4 is a suppressor of adult heart phenotype of Scer\GAL4^tin.CΔ4, Scox^GD898

(Martínez-Morentin et al., 2015)

p53^5A-1-4/p53^5A-1-4 is a suppressor of adult heart muscle cell phenotype of Scer\GAL4^tin.CΔ4, Scox^GD898

(Martínez-Morentin et al., 2015)

p53^5A-1-4/p53^5A-1-4 is a suppressor of cardiac myofibril | adult stage phenotype of Scer\GAL4^tin.CΔ4, Scox^GD898

(Martínez-Morentin et al., 2015)

p53^5A-1-4/p53^11-1B-1 is a suppressor | partially of neuroblast | larval stage phenotype of Fen1^EY12786

(Kuang et al., 2014)

p53^5A-1-4 is a suppressor of wing disc | somatic clone phenotype of Myc^αTub84B.PBb

(de la Cova et al., 2014)

p53^5A-1-4/p53^5A-1-4 is a suppressor of wing phenotype of Fcp1^RNAi.UAS.WIZ, Scer\GAL4^vg.PU

(Schauer et al., 2009)

p53^5A-1-4 is a suppressor | partially of wing disc phenotype of dup^{hsp70.Tag:MYC}

(Mehrotra et al., 2008)

p53^5A-1-4 is a suppressor | partially of follicle cell | oogenesis stage S5 phenotype of dup^{hsp70.Tag:MYC}

(Mehrotra et al., 2008)

p53^5A-1-4 is a suppressor of eye disc phenotype of Rbf^120a

(Moon et al., 2008)

p53^5A-1-4/p53^5A-1-4 is a suppressor of eye phenotype of E(mus304)[+]/PolA1^Emus304, mei-41^29D

(LaRocque et al., 2007)

p53^5A-1-4 is a suppressor of prothoracic leg disc phenotype of Scer\GAL4^Act5C.PI, wg^UAS.cLa

(Wells et al., 2006)

p53^5A-1-4/p53^5A-1-4 is a suppressor of rhabdomere of eye photoreceptor cell | progressive phenotype of Hsap\HTT^GMR.Q120

(Bae et al., 2005)

p53^5A-1-4/p53^5A-1-4 is a suppressor of eye photoreceptor cell | progressive phenotype of Hsap\HTT^GMR.Q120

(Bae et al., 2005)

NOT Suppressor of

Statement

Reference

p53^5A-1-4/p53^5A-1-4 is a non-suppressor of wing disc dorsal compartment | third instar larval stage phenotype of Scer\GAL4^ap-md544, pnut^GD1512

(Gerlach et al., 2018)

p53^5A-1-4/p53^5A-1-4 is a non-suppressor of wing disc | third instar larval stage phenotype of Ipk2^20B

(Seeds et al., 2015)

p53^5A-1-4 is a non-suppressor of mitochondrion | somatic clone phenotype of Myc^αTub84B.PBb

(de la Cova et al., 2014)

p53^5A-1-4/p53^5A-1-4 is a non-suppressor of eye disc phenotype of Scer\GAL4^ey.PU, vito^GD10890

(Marinho et al., 2011)

p53^5A-1-4/p53^11-1B-1 is a non-suppressor of wing disc phenotype of Myc^UAS.cZa, Scer\GAL4^ap-md544

(Montero et al., 2008)

p53^5A-1-4 is a non-suppressor of eye disc phenotype of Rbf^120a

(Moon et al., 2008)

p53^5A-1-4 is a non-suppressor of eye disc posterior to the morphogenetic furrow phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.PF

(Moon et al., 2008)

p53^5A-1-4 is a non-suppressor of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Other

Statement

Reference

okr^AA/okr^RU, p53^5A-1-4 has oocyte phenotype

(Chakravarti et al., 2022)

okr^AA/okr^RU, p53^5A-1-4 has nurse cell phenotype

(Chakravarti et al., 2022)

Clbn^1Q, p53^5A-1-4 has wing disc | third instar larval stage | conditional phenotype

(Wang et al., 2013)

BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4, hid^UAS.cYa, p53^5A-1-4 has wing disc phenotype

(Wells et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

p53^5A-1-4 homozygosity does not suppress the apoptosis in the third instar larval wing disc dorsal compartment induced by the expression of pnut^GD1512 from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts].

(Gerlach et al., 2018)

p53^5A-1-4 is epistatic to Corp^95B in the BARTL (Bar and Telomere Loss) assay; double mutant flies have almost wild-type eyes in this assay, indicating suppression of the eye ablation phenotype resulting from an irreparable DNA double-stranded break, as occurs in p53^5A-1-4 single mutants.

p53^5A-1-4 suppresses the increase in irradiation-induced apoptosis seen in Corp^95B mutant flies, with the double mutant phenotype being indistinguishable from that of p53^5A-1-4 single mutants.

p53^5A-1-4 suppresses the reduced eye phenotypes caused by expression of hid^GMR.PG.

(Chakraborty et al., 2015)

The apoptosis that occurs at the interface of wild type and M(2)56F^H/+ cells in M(2)56F^H/+ wing discs in which wild type clones have been induced is not significantly lowered in discs that are also mutant for p53^11-1B-1/p53^5A-1-4.

(Kale et al., 2015)

Presence of p53^5A-1-4/p53^5A-1-4 suppresses heart structural defects seen in Scer\GAL4^tin.CΔ4>Scox^GD898 flies: there are no obvious morphological heart defects (including correct alignment of cardiomyocyte myofibrils).

(Martínez-Morentin et al., 2015)

p53^5A-1-4/p53^5A-1-4 does not suppress increased cell death seen in Ipk2^20B/Ipk2^20B third instar larval wing discs.

(Seeds et al., 2015)

One copy of p53^5A-1-4 does not suppress the eye roughness seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU.

(Fan et al., 2014)

p53^5A-1-4/p53^11-1B-1 partially suppresses the neuroblast loss seen in Fen1^EY12786 mutant larval brains.

(Kuang et al., 2014)

Clbn^1Q/Clbn^1Q p53^5A-1-4/p53^5A-1-4 double mutants show the same degree of radiation sensitivity (increase in the number of breaks per nucleus in mitotic chromosomes in third instar larval brains compared to wild type) as Clbn^1Q/Clbn^1Q or p53^5A-1-4/p53^5A-1-4 mutants alone. Clbn^1Q/Clbn^1Q p53^5A-1-4/p53^5A-1-4 double mutant third instar larval wing discs appear to have almost a complete block of cell death (apoptosis) in response to irradiation. p53^5A-1-4/p53^5A-1-4 significantly enhances melanotic tumor formation in tefu^stg/tefu^stg third instar larvae (29.5%).

(Wang et al., 2013)

p53^5A-1-4/+ does not suppress the necrosis seen in stage P5 ade2^1-6 pupae.

(Holland et al., 2011)

Homozygosity for p53^5A-1-4 in the Scer\GAL4^ey.PU, vito^GD10890 background does not rescue the increased apoptosis phenotype or eye disc size.

(Marinho et al., 2011)

A loss of heterozygosity assay, using mwh¹ as a marker, reveals that a Su(var)2-10⁰³⁶⁹⁷, lwr⁰⁵⁴⁸⁶, or smt3^k06307 heterozygous background increases the number of mwh¹ clones after irradiation with 250 rad of X-rays, in p53^5A-1-4 mutants.

(Pardi et al., 2011)

p53^5A-1-4 partially suppresses the increased levels of apoptosis seen in mitotically cycling wing disc cells and ovarian follicle cells following expression of dup^{hsp70.T:Hsap\MYC}.

(Mehrotra et al., 2008)

The increased apoptosis that is seen in wing discs expressing dm^Scer\UAS.cZa under the control of Scer\GAL4^ap-md544 is not reduced by p53^5A-1-4/p53^11-1B-1.

(Montero et al., 2008)

The apoptosis in the eye disc induced by the co-expression of E2f^Scer\UAS.cNa and Dp^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PF is unaffected by homozygous p53^5A-1-4.

The wave of apoptosis that appears in the absence of irradiation in Rbf^120a mutant eye discs is unaffected by p53^5A-1-4.

The elevated level of DNA-damage induced apoptosis in Rbf^120a mutant eye discs is suppressed by p53^5A-1-4.

(Moon et al., 2008)

p53^5A-1-4/p53^5A-1-4 completely suppresses the rough eye phenotype of DNApol-α180^Emus304/+ ; mei-41^29D/Y flies. The increased level of apoptosis seen in DNApol-α180^Emus304/+ ; mei-41^29D/Y wing discs is restored by p53^5A-1-4/p53^5A-1-4 to the level seen in mei-41^29D/Y single mutants.

(LaRocque et al., 2007)

The cell cycle arrest and undergrowth phenotype of 96 hour wing discs with Scer\GAL4^hh-Gal4>W^Scer\UAS.cYa, BacA\p35^Scer\UAS.cHa cells in the posterior is completely alleviated in a p53^5A-1-4 background.

While 59% of Scer\GAL4^Act5C.PI>wg^Scer\UAS.cLa prothoracic leg discs form a blastema, only 22% of Scer\GAL4^Act5C.PI>wg^Scer\UAS.cLa, p53^5A-1-4 legs form a blastema.

(Wells et al., 2006)

Double mutants of p53^5A-1-4 and lok^p6 display similar levels of acridine orange staining (indicating cell death) after radiation (visualised in third instar imaginal discs irradiated with 4,000 R).

(Wichmann et al., 2006)

p53^5A-1-4 Ada2b² double mutant third instar wing discs show a small amount of apoptosis in the absence of irradiation, but there is no increase in apoptosis in these discs following irradiation.

(Qi et al., 2004)

Xenogenetic Interactions

Statement

Reference

The loss of germline stem cells (GSCs) observed upon heat-shock induced expression of Crei\I-CreI^hs.PR (to introduce DNA damage) is slightly alleviated by combination with a single copy of p53^5A-1-4 but is actually made worse by p53^5A-1-4 homozygosity.

(Ma et al., 2016)

p53^5A-1-4 enhances the neurodegeneration phenotype induced by expression of Scer\GAL4^elav.PLu>Hsap\MAPT^{R406W.Scer\UAS}.

(Merlo et al., 2014)

Expression of BacA\p35^GMR.PH in either wild-type or p53^5A-1-4 mutant cell clones does not affect clonal growth in controls.

Preventing apoptosis, through the presence of BacA\p35^GMR.PH, allows p53^5A-1-4, dm^αTub84B.PBb mutant clones to grow to the same size, and at the same rate, as dm^αTub84B.PBb-expressing super-competitor clones. Thus, the inability of the p53^5A-1-4, dm^αTub84B.PBb super-competitor population to expand is due to cell death that requires the presence of nearby wild-type cells.

(de la Cova et al., 2014)

p53^5A-1-4/p53^5A-1-4 significantly partially suppresses age-dependent degeneration of rhabdomeres and photoreceptor cells in Hsap\HTT^GMR.Q120 flies.

(Bae et al., 2005)

Complementation and Rescue Data

Rescued by

p53^5A-1-4 is rescued by Scer\GAL4^{VP16.nanos.UTR}/p53^UASp.A

(Park et al., 2019)

p53^5A-1-4 is rescued by p53^UASp.B/Scer\GAL4^{VP16.nanos.UTR}

(Park et al., 2019)

p53^5A-1-4 is rescued by p53^+t21

(Robin et al., 2019)

p53^5A-1-4 is rescued by p53^{+tCH322-178C12}

(Zhang et al., 2015)

p53^5A-1-4 is rescued by p53^{+tCH322-178C12}

(Zhang et al., 2014)

p53^5A-1-4 is rescued by p53^mCherry

(Zhang et al., 2014)

p53^5A-1-4 is rescued by p53^+tTa

(Titen and Golic, 2008)

Partially rescued by

p53^5A-1-4 is partially rescued by Scer\GAL4^nanos.PG/p53^UASp.A

(Park et al., 2019)

Not rescued by

p53^5A-1-4 is not rescued by p53^ASTOP.GFP

(Zhang et al., 2015)

Comments

Expression of p53^UASp.B under the control of Scer\GAL4^VP16.nos.UTR rescues the cell death in germarium after 3 Gy irradiation phenotype and the daily egg production 3 days after 40 Gy irradiation phenotype; it partially rescues the number of germline stem cells after 40 Gy irradiation phenotype; and it fails to rescue the fertility (number of pupa produced per day) after 40 Gy irradiation phenotype of p53^5A-1-4 females.

Expression one copy of p53^UASp.A under the control one copy of Scer\GAL4^nos.PG partially rescues while expression of two copies of p53^UASp.A under the control of Scer\GAL4^nos.PG (one or two copies) fails to recue the fertility (number of pupa produced per day) after 40 Gy irradiation phenotype of p53^5A-1-4 females.

Expression of p53^UASp.A (one or two copies) under the control of Scer\GAL4^nos.PG (one or two copies) partially rescues the number of germline stem cells after 40 Gy irradiation phenotype of p53^5A-1-4 females.

(Park et al., 2019)

Expression of p53^+tTa rescues the partial suppression of apoptosis seen in p53^5A-1-4 mutant cells containing dicentric chromosomes (generated using Scer\FLP1^hs.PP-induced recombination in chromosomes containing inverted Scer\FRT sites).

(Titen and Golic, 2008)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6815

y¹ w¹¹¹⁸; p53^5A-1-4

99703

Dp(1;Y)Bar^S; dsx¹ p^p p53^5A-1-4/TM3, Sb¹

Notes on Origin

Discoverer

Comments

Generated in screen described in FBrf0149003.

(Golic, 2002.10.25)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

5A-1-4

(Mukherjee et al., 2006)

Dmp53^5A-1-4

(Gowda et al., 2012, Pardi et al., 2011, Schauer et al., 2009, Bodai et al., 2007)

Dp53^5A-1-4

(Simón et al., 2014)

P53^5A-1-4

(Ji et al., 2021, Bae et al., 2005)

dp53^-4

(Moon et al., 2008)

dp53^4-

(Morris et al., 2006)

dp53^5A-1-4

(D'Brot et al., 2017, Montero et al., 2008)

dp53^5a-1-4

(Wells et al., 2006)

p53 5a-1-4

(Gerlach et al., 2018)

p53 ^5A-1-4

(Dichtel-Danjoy et al., 2013)

p53-LOF

(Martin et al., 2021)

p53^5A-1-4

(Jansen et al., 2024, Vasavan et al., 2024, Kim et al., 2023, Chakravarti et al., 2022, Easwaran et al., 2022, Martin et al., 2022, Chang et al., 2021, Dong et al., 2021, Li et al., 2021, Tarayrah-Ibraheim et al., 2021, Miller et al., 2020, Park et al., 2019, Robin et al., 2019, Wei et al., 2019, Ma et al., 2017, Napoletano et al., 2017, Wang et al., 2017, Flegel et al., 2016, Ma et al., 2016, Chakraborty et al., 2015, Kale et al., 2015, Martínez-Morentin et al., 2015, Molla-Herman et al., 2015, Seeds et al., 2015, Zhang et al., 2015, Fan et al., 2014, Kuang et al., 2014, Merlo et al., 2014, Zhang et al., 2014, Wang et al., 2013, Edwards et al., 2011, Holland et al., 2011, Kurzhals et al., 2011, Mehrotra et al., 2008, Titen and Golic, 2008, Yamada et al., 2008, LaRocque et al., 2007, Colombani et al., 2006, Wichmann et al., 2006, McEwen and Peifer, 2005, Gorski et al., 2004)

p53^5A-14

(Beaucher et al., 2012)

p53^5A1-4

(Robin et al., 2019)

p53^5A14

(Barrio et al., 2014)

p53^5A

(Ma et al., 2017)

p53^5a-1-4

(de la Cova et al., 2014)

p53^5a1.4

(Martin et al., 2021)

p53^<up>5A-1-4</up>

(Marinho et al., 2011)

p53^A-1-4

(Iampietro et al., 2014)

p53[5A-1-4]

(Shcherbata et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (70)

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