FlyBase Allele Report: Dmel\p53[11-1B-1]

General Information

Symbol

Dmel\p53^11-1B-1

Species

D. melanogaster

Name

FlyBase ID

FBal0138189

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

Also Known As

p53¹, dp53^11-1B-1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Golic, 2002.10.25)

ends-in gene targeting

(Rong et al., 2002)

FLPase

(Rong et al., 2002)

I-CreI

(Rong et al., 2002)

I-SceI

(Rong et al., 2002)

Progenitor genotype

Cytology

Description

A stop codon has been inserted into the p53 coding region.

(Rong et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:23,051,088..23,051,088 [-]

Nucleotide change:

C23051088T

Reported nucleotide change:

C?T

Amino acid change:

Q74term | p53-PA; Q184term | p53-PB; Q74term | p53-PC; Q23term | p53-PE

Reported amino acid change:

??term

Comment:

Site of nonsense mutation deduced from primer sequences provided in reference.

(Golic, 2002.10.25)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alexander disease

modeled by Hsap\GFAP^R79H.UAS

(Wang et al., 2015)

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Merlo et al., 2014)

ameliorates brain ischemia

modeled by Mmus\Gria1^Lc.UAS

(Lei et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Brodsky et al., 2004)

increased cell death | conditional

(Jassim et al., 2003)

viable

(Mandal et al., 2005, Brodsky et al., 2004, Rong et al., 2002)

Phenotype Manifest In

eye | conditional

(Jassim et al., 2003)

oocyte | oogenesis stage S2 (with p53²)

(Lu et al., 2010)

oocyte | oogenesis stage S2 (with p53^-ns)

(Lu et al., 2010)

primordial germ cell

(Yamada et al., 2008)

Detailed Description

Statement

Reference

p53^11-1B-1 mutant flies show no evidence of neurodegeneration.

(Merlo et al., 2014)

p53^11-1B-1/+ flies do not show any visible wing defects compared to wild type controls.

(Simón et al., 2014)

Meiotic recombination frequency is reduced in p53^11-1B-1/p53^-ns mutants compared with wild type.

Meiotic recombination frequency is reduced in p53^11-1B-1/p53² mutants compared with wild type.

(Lu et al., 2010)

Programmed cell death of primordial germ cells (PGCs), which is essentially complete by stage 12 of embryogenesis in wild type, is disrupted in mutant embryos; the total average number of PGCs is normal in the mutants at stage 10, but although there is a gradual reduction in the number of PGCs, it is decreased compared to wild type, such that at stage 14 the average number of PGCs is higher in the mutant embryos compared to wild type.

Although PGCs are able to incorporate into the gonad in p53^11-1B-1 embryos, an average of 9.1 germ cells ectopic to the gonad are seen at stage 14 (compared to the wild-type average of 0.4 ectopic germ cells at this stage).

(Yamada et al., 2008)

The wing discs of p53^11-1B-1 mutant third instar larvae exhibit reduced levels of apoptosis in response to X-ray induced irradiation with 4000 rads compared to controls. As in controls, very little apoptosis is seen in the absence of irradiation and cell cycle arrest occurs at G2.

(Brodsky et al., 2004)

Homozygous mutants exhibit an enhanced retinal sensitivity to UV light and an increase in apoptotic cell death. Whilst 30 minutes of light-induced photorepair is enough to rescue wild-type animals, it is not enough for mutants.

(Jassim et al., 2003)

Shows no obvious phenotype.

(Golic, 2002.10.25)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

p53^11-1B-1 has increased cell death | conditional phenotype, enhanceable by mus201^D1

(Jassim et al., 2003)

Enhancer of

Statement

Reference

p53^11-1B-1 is an enhancer of increased cell death phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Merlo et al., 2014)

p53^11-1B-1 is an enhancer of abnormal mitotic cell cycle phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Merlo et al., 2014)

p53^11-1B-1 is an enhancer of abnormal locomotor behavior phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Merlo et al., 2014)

p53[+]/p53^11-1B-1 is an enhancer of visible | adult stage | dominant phenotype of wg¹

(Simón et al., 2014)

p53[+]/p53^11-1B-1 is an enhancer of visible | adult stage | dominant phenotype of N^55e11

(Simón et al., 2014)

p53^11-1B-1 is an enhancer of increased cell death | conditional phenotype of mus201^D1

(Jassim et al., 2003)

Suppressor of

Statement

Reference

p53^11-1B-1 is a suppressor of increased cell death | adult stage phenotype of Hsap\GFAP^R79H.UAS, Scer\GAL4^repo.PU

(Wang et al., 2015)

p53^5A-1-4/p53^11-1B-1 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Fen1^EY12786

(Kuang et al., 2014)

p53^11-1B-1/p53^11-1B-1 is a suppressor of decreased occurrence of cell division | somatic clone | larval stage phenotype of COX5A^tend

(Mandal et al., 2010)

p53^11-1B-1 is a suppressor of abnormal mitotic cell cycle phenotype of COX5A^tend

(Mandal et al., 2005)

p53^11-1B-1 is a suppressor of increased cell death phenotype of tefu^Δ356

(Oikemus et al., 2004)

NOT Suppressor of

Statement

Reference

p53^11-1B-1 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PU, mir-285^{UAS.DsRed(Unk)}

(Li et al., 2017)

p53^5A-1-4/p53^11-1B-1 is a non-suppressor of increased cell death | somatic clone phenotype of M(2)56F^H

(Kale et al., 2015)

p53^11-1B-1 is a non-suppressor of sterile phenotype of Rpp30^18.2

(Molla-Herman et al., 2015)

p53^5A-1-4/p53^11-1B-1 is a non-suppressor of increased cell death phenotype of Myc^UAS.cZa, Scer\GAL4^ap-md544

(Montero et al., 2008)

p53^11-1B-1 is a non-suppressor of lethal | recessive | pupal stage phenotype of tefu^Δ356

(Oikemus et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

p53^11-1B-1 has eye | conditional phenotype, enhanceable by mus201^D1

(Jassim et al., 2003)

Enhancer of

Statement

Reference

p53^11-1B-1 is an enhancer of brain phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Merlo et al., 2014)

p53[+]/p53^11-1B-1 is an enhancer of wing phenotype of wg¹

(Simón et al., 2014)

p53[+]/p53^11-1B-1 is an enhancer of wing phenotype of N^55e11

(Simón et al., 2014)

p53^11-1B-1 is an enhancer of eye | conditional phenotype of mus201^D1

(Jassim et al., 2003)

Suppressor of

Statement

Reference

p53^11-1B-1 is a suppressor of egg chamber phenotype of Rpp30^18.2

(Molla-Herman et al., 2015)

p53^11-1B-1 is a suppressor of adult brain phenotype of Hsap\GFAP^R79H.UAS, Scer\GAL4^repo.PU

(Wang et al., 2015)

p53^5A-1-4/p53^11-1B-1 is a suppressor | partially of neuroblast | larval stage phenotype of Fen1^EY12786

(Kuang et al., 2014)

p53[+]/p53^11-1B-1 is a suppressor of eye | somatic clone phenotype of COX5A^tend

(Mandal et al., 2010)

p53^11-1B-1 is a suppressor of eye | somatic clone phenotype of COX5A^tend

(Mandal et al., 2005)

p53^11-1B-1 is a suppressor of lens | somatic clone phenotype of COX5A^tend

(Mandal et al., 2005)

p53^11-1B-1 is a suppressor of wing disc phenotype of tefu^Δ356

(Oikemus et al., 2004)

NOT Suppressor of

Statement

Reference

p53^11-1B-1 is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, mir-285^{UAS.DsRed(Unk)}

(Li et al., 2017)

p53^5A-1-4/p53^11-1B-1 is a non-suppressor of wing disc phenotype of Myc^UAS.cZa, Scer\GAL4^ap-md544

(Montero et al., 2008)

p53^11-1B-1 is a non-suppressor of egg chorion phenotype of okr^AA/okr^RU

(Abdu et al., 2002)

p53^11-1B-1 is a non-suppressor of dorsal appendage phenotype of okr^AA/okr^RU

(Abdu et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The apoptosis that occurs at the interface of wild type and M(2)56F^H/+ cells in M(2)56F^H/+ wing discs in which wild type clones have been induced is not significantly lowered in discs that are also mutant for p53^11-1B-1/p53^5A-1-4.

(Kale et al., 2015)

Some egg chambers are found developing to late stages of oogenesis or even forming eggs in Rpp30^18.2 ; p53^11-1B-1 double mutant females. This rescue is partial, as the majority of egg chambers are still arrested early and the flies are still sterile.

(Molla-Herman et al., 2015)

One copy of p53^11-1B-1 does not suppress the eye roughness seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU.

(Fan et al., 2014)

p53^5A-1-4/p53^11-1B-1 partially suppresses the neuroblast loss seen in Fen1^EY12786 mutant larval brains.

(Kuang et al., 2014)

A p53^11-1B-1 background suppresses the cell division disruption found in CoVa^tend mutant eye disc clones.

(Mandal et al., 2010)

The increased apoptosis that is seen in wing discs expressing dm^Scer\UAS.cZa under the control of Scer\GAL4^ap-md544 is not reduced by p53^5A-1-4/p53^11-1B-1.

(Montero et al., 2008)

The 'glossy' eye phenotype due to CoVa^tend homozygous clones is largely suppressed if the clones are generated in a p53^11-1B-1 homozygous background. The cell cycle defects seen in CoVa^tend homozygous clones in the eye disc are also suppressed by in this background.

(Mandal et al., 2005)

p53^11-1B-1, mus201^D1 double heterozygotes exhibit an enhanced retinal sensitivity to UV light and an increase in apoptotic cell death.

(Jassim et al., 2003)

Xenogenetic Interactions

Statement

Reference

p53^11-1B-1 significantly suppresses cell death in flies with expression of Hsap\GFAP^{R79H.Scer\UAS} driven by Scer\GAL4^repo.PU.

(Wang et al., 2015)

p53^11-1B-1 enhances the neurodegeneration phenotype induced by expression of Scer\GAL4^elav.PLu>Hsap\MAPT^{R406W.Scer\UAS}.

(Merlo et al., 2014)

Complementation and Rescue Data

Rescued by

p53^11-1B-1 is rescued by p53^{+tCH322-178C12}

(Zhang et al., 2014)

p53^11-1B-1 is rescued by p53^mCherry

(Zhang et al., 2014)

p53^11-1B-1 is rescued by p53^GUS.PB

(Brodsky et al., 2004)

Comments

Expression of p53^GUS.PB rescues the reduction in X-ray induced radiation seen in p53^11-1B-1 mutant third instar larvae. This rescue is accomplished in the absence of a Scer\GAL4 driver.

(Brodsky et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6816

y¹ w¹¹¹⁸; p53^11-1B-1

Notes on Origin

Discoverer

Comments

Generated in screen described in FBrf0149003.

(Golic, 2002.10.25)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Dp53^11-1B-1

(Simón et al., 2014)

dp53^11-1B-1

(Montero et al., 2008)

p53^11-1B-1

(Jansen et al., 2024, Martin et al., 2022, Li et al., 2017, Ma et al., 2017, Flegel et al., 2016, Kale et al., 2015, Molla-Herman et al., 2015, Wang et al., 2015, Fan et al., 2014, Kuang et al., 2014, Merlo et al., 2014, Zhang et al., 2014, Wang et al., 2013, Kurzhals et al., 2011, Mandal et al., 2010, Yamada et al., 2008)

p53¹

(Lei et al., 2017, Papaconstantinou et al., 2010, Brodsky et al., 2004, Oikemus et al., 2004, Abdu et al., 2002)

p53^1B

(Ma et al., 2017)

p53^<up>1</up>

(Lu et al., 2010)

p53[11-1B-1]

(Shcherbata et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (30)

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