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General Information
Symbol
Dmel\p5311-1B-1
Species
D. melanogaster
Name
FlyBase ID
FBal0138189
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
p531, dp5311-1B-1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C23051088T

Reported nucleotide change:

C?T

Amino acid change:

Q74term | p53-PA; Q184term | p53-PB; Q74term | p53-PC; Q23term | p53-PE

Reported amino acid change:

??term

Comment:

Site of nonsense mutation deduced from primer sequences provided in reference.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A stop codon has been inserted into the p53 coding region.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

p5311-1B-1 mutant flies show no evidence of neurodegeneration.

p5311-1B-1/+ flies do not show any visible wing defects compared to wild type controls.

Meiotic recombination frequency is reduced in p5311-1B-1/p53-ns mutants compared with wild type.

Meiotic recombination frequency is reduced in p5311-1B-1/p532 mutants compared with wild type.

Programmed cell death of primordial germ cells (PGCs), which is essentially complete by stage 12 of embryogenesis in wild type, is disrupted in mutant embryos; the total average number of PGCs is normal in the mutants at stage 10, but although there is a gradual reduction in the number of PGCs, it is decreased compared to wild type, such that at stage 14 the average number of PGCs is higher in the mutant embryos compared to wild type.

Although PGCs are able to incorporate into the gonad in p5311-1B-1 embryos, an average of 9.1 germ cells ectopic to the gonad are seen at stage 14 (compared to the wild-type average of 0.4 ectopic germ cells at this stage).

The wing discs of p5311-1B-1 mutant third instar larvae exhibit reduced levels of apoptosis in response to X-ray induced irradiation with 4000 rads compared to controls. As in controls, very little apoptosis is seen in the absence of irradiation and cell cycle arrest occurs at G2.

Homozygous mutants exhibit an enhanced retinal sensitivity to UV light and an increase in apoptotic cell death. Whilst 30 minutes of light-induced photorepair is enough to rescue wild-type animals, it is not enough for mutants.

Shows no obvious phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference

p5311-1B-1 has eye | conditional phenotype, enhanceable by mus201D1

Enhancer of
Statement
Reference

p53[+]/p5311-1B-1 is an enhancer of wing phenotype of wg1

p53[+]/p5311-1B-1 is an enhancer of wing phenotype of N55e11

p5311-1B-1 is an enhancer of eye | conditional phenotype of mus201D1

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

p5311-1B-1 is a non-suppressor of egg chorion phenotype of okrAA/okrRU

p5311-1B-1 is a non-suppressor of dorsal appendage phenotype of okrAA/okrRU

Additional Comments
Genetic Interactions
Statement
Reference

The apoptosis that occurs at the interface of wild type and M(2)56FH/+ cells in M(2)56FH/+ wing discs in which wild type clones have been induced is not significantly lowered in discs that are also mutant for p5311-1B-1/p535A-1-4.

Some egg chambers are found developing to late stages of oogenesis or even forming eggs in Rpp3018.2 ; p5311-1B-1 double mutant females. This rescue is partial, as the majority of egg chambers are still arrested early and the flies are still sterile.

One copy of p5311-1B-1 does not suppress the eye roughness seen when SRm160GE25979 is expressed under the control of Scer\GAL4GMR.PU.

p535A-1-4/p5311-1B-1 partially suppresses the neuroblast loss seen in Fen1EY12786 mutant larval brains.

A p5311-1B-1 background suppresses the cell division disruption found in CoVatend mutant eye disc clones.

The increased apoptosis that is seen in wing discs expressing dmScer\UAS.cZa under the control of Scer\GAL4ap-md544 is not reduced by p535A-1-4/p5311-1B-1.

The 'glossy' eye phenotype due to CoVatend homozygous clones is largely suppressed if the clones are generated in a p5311-1B-1 homozygous background. The cell cycle defects seen in CoVatend homozygous clones in the eye disc are also suppressed by in this background.

p5311-1B-1, mus201D1 double heterozygotes exhibit an enhanced retinal sensitivity to UV light and an increase in apoptotic cell death.

Xenogenetic Interactions
Statement
Reference

p5311-1B-1 significantly suppresses cell death in flies with expression of Hsap\GFAPR79H.Scer\UAS driven by Scer\GAL4repo.PU.

p5311-1B-1 enhances the neurodegeneration phenotype induced by expression of Scer\GAL4elav.PLu>Hsap\MAPTR406W.Scer\UAS.

Complementation and Rescue Data
Comments

Expression of p53GUS.PB rescues the reduction in X-ray induced radiation seen in p5311-1B-1 mutant third instar larvae. This rescue is accomplished in the absence of a Scer\GAL4 driver.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Generated in screen described in FBrf0149003.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (25)