Allele Dmel\imd^Scer\UAS.cGa

General Information
Symbol	Dmel\imd^Scer\UAS.cGa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Georgel	FlyBase ID	FBal0138219
Feature type	allele	Associated gene	Dmel\imd

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Leulier et al., 2002, Georgel et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of imd. (Leulier et al., 2002) Construct: Expression of the complete imd cDNA (GH20785) is under the control of Scer\UAS sequences. (Georgel et al., 2001)
Carried in construct	P{UAS-imd.G} (Kleino et al., 2005, Georgel et al., 2001, Kim et al., 2006, Leulier et al., 2002, Foley and O'Farrell, 2003, Agaisse et al., 2003, Pal et al., 2007, Akhouayri et al., 2011, Wu et al., 2007, Buchon et al., 2009, Leulier et al., 2006, Thevenon et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death, with Scer\GAL4^hs.PB (Georgel et al., 2001) lethal \| larval stage, with Scer\GAL4^da.G32 (Georgel et al., 2001) radiation sensitive, with Scer\GAL4^hs.PB (Georgel et al., 2001) viable, with Scer\GAL4^hs.PB (Georgel et al., 2001)
Phenotype Manifest In
	embryonic/larval fat body, with Scer\GAL4^hs.PB (Georgel et al., 2001)
Detailed Description
	Statement Reference Expression of imd^Scer\UAS.cGa under the control of Scer\GAL4^c564 increases the survival of flies that have been injected with both E.coli and the fungal peptide Destruxin A. (Pal et al., 2007) The fat bodies of imd^Scer\UAS.cGa; Scer\GAL4^hs.PB flies have increased levels of cell death compared to wild-type. The proportion of imd^Scer\UAS.cGa; Scer\GAL4^hs.PB pupae (24 hours APF) surviving to adulthood after UV irradiation (50,000 μJ/cm²) is significantly lower than for wild-type. (Georgel et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^da.G32, imd^Scer\UAS.cGa has lethal \| larval stage phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^da.G32 (Georgel et al., 2001) Scer\GAL4^hs.PB, imd^Scer\UAS.cGa has increased cell death phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^hs.PB (Georgel et al., 2001)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^da.G32, imd^Scer\UAS.cGa has embryonic/larval fat body phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^da.G32 (Georgel et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference The larval lethality of imd^Scer\UAS.cGa; Scer\GAL4^da.G32 is rescued by BacA\p35^Scer\UAS.cHa. Increased levels of cell death seen in the fact bodies of imd^Scer\UAS.cGa; Scer\GAL4^hs.PB flies are suppressed by BacA\p35^Scer\UAS.cHa. (Georgel et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	imd^Scer\UAS.cGa/Scer\GAL4^hs.PB rescues imd¹ (Georgel et al., 2001)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	imd^Scer\UAS.cGa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Georgel (Leulier et al., 2002)
Secondary FlyBase IDs

References ( 12 )
Research paper	Akhouayri et al., 2011, PLoS Pathog. 7(10): e1002319 Toll-8/tollo negatively regulates antimicrobial response in the Drosophila respiratory epithelium. [FBrf0216452] Buchon et al., 2009, Cell Host Microbe 5(2): 200--211 Drosophila intestinal response to bacterial infection: activation of host defense and stem cell proliferation. [FBrf0206694] Thevenon et al., 2009, Cell Host Microbe 6(4): 309--320 The Drosophila ubiquitin-specific protease dUSP36/Scny targets IMD to prevent constitutive immune signaling. [FBrf0208983] Pal et al., 2007, J. Biol. Chem. 282(12): 8969--8977 Fungal peptide destruxin a plays a specific role in suppressing the innate immune response in Drosophila melanogaster. [FBrf0200357] Wu et al., 2007, Cell. Microbiol. 9(4): 1073--1085 ird1 is a Vps15 homologue important for antibacterial immune responses in Drosophila. [FBrf0201559] Kim et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103(44): 16358--16363 Caspar, a suppressor of antibacterial immunity in Drosophila. [FBrf0194794] Leulier et al., 2006, Mol. Cell. Biol. 26(21): 7821--7831 The Drosophila inhibitor of apoptosis protein DIAP2 functions in innate immunity and is essential to resist gram-negative bacterial infection. [FBrf0192381] Kleino et al., 2005, EMBO J. 24(19): 3423--3434 Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Drosophila Imd pathway. [FBrf0190362] Agaisse et al., 2003, Dev. Cell 5(3): 441--450 Signaling role of hemocytes in Drosophila JAK/STAT-dependent response to septic injury. [FBrf0162064] Foley and O'Farrell, 2003, Genes Dev. 17(1): 115--125 Nitric oxide contributes to induction of innate immune responses to gram-negative bacteria in Drosophila. [FBrf0155482] Leulier et al., 2002, Curr. Biol. 12(12): 996--1000 Inducible expression of double-stranded RNA reveals a role for dFADD in the regulation of the antibacterial response in Drosophila adults. [FBrf0151243] Georgel et al., 2001, Dev. Cell 1(4): 503--514 Drosophila immune deficiency (IMD) is a death domain protein that activates antibacterial defense and can promote apoptosis. [FBrf0141455]

Allele Dmel\imdScer\UAS.cGa

Allele Dmel\imd^Scer\UAS.cGa