Allele Dmel\e^Scer\UAS.cWa

General Information
Symbol	Dmel\e^Scer\UAS.cWa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Wittkopp	FlyBase ID	FBal0138233
Feature type	allele	Associated gene	Dmel\e

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Wittkopp et al., 2002)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A full length 1.9kb e cDNa is driven by Scer\UAS sequences. (Wittkopp et al., 2002)
Carried in construct	P{UAS-e.W} (Wittkopp et al., 2002, Wittkopp et al., 2009, Sekine et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	body color defective, with Scer\GAL4^pnr-MD237 (Wittkopp et al., 2002, Wittkopp et al., 2009)
Phenotype Manifest In
	adult cuticle, with Scer\GAL4^pnr-MD237 (Wittkopp et al., 2009)
Detailed Description
	Statement Reference Expression of e[Scer\UAS.cWa] under the control of Scer\GAL4[pnr-MD237] causes increased yellow pigmentation in the abdominal cuticle. (Wittkopp et al., 2009) e^Scer\UAS.cWa when driven by Scer\GAL4^pnr-MD237, suppresses black pigment formation in all epidermal cells. In the thorax the cuticle lightens slightly relative to wild-type. In the abdomen a striking inhibition of melanin formation is seen, which is replaced with a tan pigment. (Wittkopp et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^pnr-MD237, e^Scer\UAS.cWa has body color defective phenotype, suppressible \| partially by y^Scer\UAS.cCa/Scer\GAL4^pnr-MD237 (Wittkopp et al., 2002)
Suppressor of
Statement Reference e^Scer\UAS.cWa, Scer\GAL4^pnr-MD237 is a suppressor of body color defective phenotype of Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 (Sekine et al., 2011)
Phenotype Manifest In
Suppressor of
Statement Reference e^Scer\UAS.cWa, Scer\GAL4^pnr-MD237 is a suppressor of adult thorax phenotype of Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 (Sekine et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of e[Scer\UAS.cWa] abolishes the thorax pigmentation defects seen in Pk92B[ΔN.Scer\UAS.T:Zzzz\FLAG], Scer\GAL4[pnr-MD237] flies. (Sekine et al., 2011) Simultaneous co-expression of e[Scer\UAS.cWa] and t[Scer\UAS.cTa] under the control of Scer\GAL4[pnr-MD237] results in a pigmentation phenotype in the abdominal cuticle that is intermediate to that caused by expression of either e[Scer\UAS.cWa] or t[Scer\UAS.cTa] alone. (Wittkopp et al., 2009) When y^Scer\UAS.cCa and e^Scer\UAS.cWa are driven together by Scer\GAL4^pnr-MD237 induces the formation of more black melanin than e^Scer\UAS.cWa alone, though the wild-type level of pigmentation is not restored. (Wittkopp et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	37297 w^*; P{UAS-e.W}88f2/TM3, Sb¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	e^Scer\UAS.cWa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Wittkopp (Wittkopp et al., 2002)
Secondary FlyBase IDs

References ( 3 )
Research paper	Sekine et al., 2011, J. Cell Sci. 124(17): 3006--3016 p38 MAPKs regulate the expression of genes in the dopamine synthesis pathway through phosphorylation of NR4A nuclear receptors. [FBrf0215011] Wittkopp et al., 2009, Science 326(5952): 540--544 Intraspecific polymorphism to interspecific divergence: genetics of pigmentation in Drosophila. [FBrf0209245] Wittkopp et al., 2002, Development 129(8): 1849--1858 Reciprocal functions of the Drosophila yellow and ebony proteins in the development and evolution of pigment patterns. [FBrf0146983]

Allele Dmel\eScer\UAS.cWa

Allele Dmel\e^Scer\UAS.cWa