FlyBase Allele Report: Dmel\Tak1[NIG.1388R]

General Information

Symbol

Dmel\Tak1^NIG.1388R

Species

D. melanogaster

Name

FlyBase ID

FBal0138376

Feature type

allele

Associated gene

Dmel\Tak1

Associated Insertion(s)

Carried in Construct

P{NIG.1388R}

Also Known As

UAS-dTAK1-IR

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Leulier et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Leulier et al., 2002)

Mutagen

in vitro construct

(Leulier et al., 2002)

Progenitor genotype

Carried in construct

P{NIG.1388R}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat of nucleotides 1-500 of the Tak1 cDNA.

(Leulier et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-1388R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates carcinoma

modeled by Ras85D^V12.UAS, l(2)gl⁴

(Igaki et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^hs.PB

(Leulier et al., 2002)

abnormal immune response, with Scer\GAL4^yolk

(Leulier et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

RNAi knockdown of Tak1, through expression of Tak1^NIG.1388R in the posterior compartment of the wing disc, under the control of either Scer\GAL4^en.PU or Scer\GAL4^hh.PU, does not affect levels of cell death.

(Wong et al., 2014)

Tak1^{dsRNA.Scer\UAS} when driven by Scer\GAL4^yolk or Scer\GAL4^hs.PB produces an immune deficiency phenotype. Mutant animals are highly susceptible to Gram-negative bacterial infection, exhibiting a dramatic loss of survival rate after infection, with all animals dying within 4 days. Expression of Tak1^{dsRNA.Scer\UAS} when driven by Scer\GAL4^da.G32 does not lead to any detectable developmental defects.

(Leulier et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, Tak1^NIG.1388R, mad2^GD16427 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, Tak1^NIG.1388R, mad2^GD16427 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

Suppressor of

Statement

Reference

Tak1^NIG.1388R/Scer\GAL4^ey.PU is a suppressor of visible phenotype of Scer\GAL4^ey.PU, ben^d09936

(Ma et al., 2014)

Tak1^NIG.1388R/Scer\GAL4^ey.PU is a suppressor of visible phenotype of Scer\GAL4^pnr.PU, ben^EP1651

(Ma et al., 2014)

Scer\GAL4^ptc-559.1/Tak1^NIG.1388R is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, Uev1A^UAS.cMa, ben^UAS.cUa

(Ma et al., 2013)

Tak1^NIG.1388R, Scer\GAL4^dpp.blk1 is a suppressor of visible phenotype of Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+]

(Neisch et al., 2010)

slpr^NIG.2272R/Tak1^NIG.1388R, Scer\GAL4^dpp.blk1 is a suppressor of increased cell death phenotype of Rho1^UAS.cMa, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Tak1^NIG.1388R/Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Ras85D^KP

(Wu et al., 2009)

Tak1^NIG.1388R, Scer\GAL4^Act5C.PI is a suppressor of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, l(2)gl⁴

(Igaki et al., 2006)

Other

Statement

Reference

Scer\GAL4^hh.PU, Tak1^NIG.1388R, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, Tak1^NIG.1388R, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, Tak1^NIG.1388R, mad2^GD16427 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, Tak1^NIG.1388R, mad2^GD16427 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

Suppressor of

Statement

Reference

Tak1^NIG.1388R/Scer\GAL4^pnr.PU is a suppressor of scutellum phenotype of Scer\GAL4^ey.PU, ben^EP1651

(Ma et al., 2014)

Tak1^NIG.1388R/Scer\GAL4^ey.PU is a suppressor of eye phenotype of Scer\GAL4^ey.PU, ben^d09936

(Ma et al., 2014)

Scer\GAL4^ptc-559.1/Tak1^NIG.1388R is a suppressor of anterior crossvein phenotype of Scer\GAL4^ptc-559.1, Uev1A^UAS.cMa, ben^UAS.cUa

(Ma et al., 2013)

Tak1^NIG.1388R, Scer\GAL4^dpp.blk1 is a suppressor of wing blade phenotype of Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+]

(Neisch et al., 2010)

slpr^NIG.2272R/Tak1^NIG.1388R, Scer\GAL4^dpp.blk1 is a suppressor of wing disc | third instar larval stage phenotype of Rho1^UAS.cMa, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Tak1^NIG.1388R/Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Ras85D^KP

(Wu et al., 2009)

Other

Statement

Reference

Scer\GAL4^hh.PU, Tak1^NIG.1388R, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, Tak1^NIG.1388R, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Tak1^NIG.1388R suppresses the rough eye phenotype seen when ben^d09936 is expressed under the control of Scer\GAL4^ey.PU.

Expression of Tak1^NIG.1388R suppresses the small scutellum phenotype seen when ben^EP1651 is expressed under the control of Scer\GAL4^pnr.PU.

(Ma et al., 2014)

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when Tak1^NIG.1388R is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. There is also a concomitant increase in cell death in these double mutants.

(Wong et al., 2014)

Expression of Tak1^NIG.1388R suppresses the anterior crossvein loss seen when Uev1A^Scer\UAS.cMa and ben^Scer\UAS.cUa are expressed under the control of Scer\GAL4^ptc-559.1.

(Ma et al., 2013)

Co-expression of both slpr^{dsRNA.Scer\UAS.NIG} and Tak1^{dsRNA.Scer\UAS} significantly suppresses the ectopic apoptosis in cells also expressing Rho1^Scer\UAS.cMa under the control of Scer\GAL4^dpp.blk1.

(Neisch et al., 2010)

The eye phenotype in Ras85D^KP mutant flies is partially suppressed by ectopic expression of Tak1^{dsRNA.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PF.

(Wu et al., 2009)

Expression of Tak1^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^Act5C.PI) using the FLP/FRT system strongly suppresses metastatic behaviour in l(2)gl⁴ mutants expressing Ras85D^V12.Scer\UAS.

(Igaki et al., 2006)

Xenogenetic Interactions

Statement

Reference

(Wong et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

1388R-1

P{NIG.1388R}1

1388R-2

P{NIG.1388R}2

Notes on Origin

Discoverer

Comments

Transcription from the P{UAS-Tak1-dsRNA} construct should produce Tak1 dsRNA, resulting in dsRNA interference (RNAi) of the Tak1 gene.

(Leulier et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tak1^NIG.1388R

Tak1^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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