FlyBase Allele Report: Dmel\Parp1[CH1]

General Information

Symbol

Dmel\Parp1^CH1

Species

D. melanogaster

Name

FlyBase ID

FBal0138435

Feature type

allele

Associated gene

Dmel\Parp1

Associated Insertion(s)

P{PZ}Parp1^CH1

Carried in Construct

Also Known As

parp^CH1, CH(3)1

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Tulin et al., 2002)

Progenitor genotype

Associated Insertion(s)

P{PZ}Parp1^CH1

Cytology

Description

P{PZ} insertion near the upstream promoter of the Parp e isoform.

(Tulin et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Pink1^B9

(Lehmann et al., 2017)

modeled by park²⁵

(Lehmann et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Tulin et al., 2002)

abnormal heat stress response

(Tulin et al., 2006)

abnormal immune response

(Tulin and Spradling, 2003)

lethal - all die before end of larval stage | recessive

(Tulin et al., 2002)

non-enhancer of variegation, with In(1)w^m4

(Tulin et al., 2002)

non-suppressor of variegation, with In(1)w^m4

(Tulin et al., 2002)

some die during larval stage | recessive

(Tulin et al., 2002)

Phenotype Manifest In

cephalopharyngeal skeleton

(Tulin et al., 2002)

nucleolus

(Tulin et al., 2006, Tulin et al., 2002)

nucleus

(Tulin et al., 2002)

polytene chromosome chromocenter

(Tulin et al., 2002)

polytene chromosome puff

(Tulin and Spradling, 2003)

Detailed Description

Statement

Reference

Parp^CH1 heterozygous mutant adults do not present altered oxygen consumption and do not present brain mitochondria with altered potential, as compared to controls.

(Lehmann et al., 2017)

Parp^CH1/+ mutants display no reduction in dopaminergic PPL1 neuron number as compared to controls.

(Lehmann et al., 2016)

Parp^CH1 cells lack nucleoli. Only 10% of Parp^CH1 third instar larvae survive a 40-min heat shock, while 99% of wild-type larvae survive.

(Tulin et al., 2006)

The average size of the heat shock puffs at 87A and 87C on the polytene chromosomes is reduced threefold in mutant larvae compared to wild type. Mutant larvae often develop intracellular bacterial infections, which is rarely seen in wild type. The larvae are also sensitive to experimental infection; 95% die after a dose of injected E.coli that kills less than 7% of controls.

(Tulin and Spradling, 2003)

Homozygotes develop slowly and usually die during the second instar stage after 6-9 days. The brain is of normal size. Up to 50% of the larvae are arrested at the onset of ecdysis II (as seen by the phenotype of the mouth hooks). Nuclear morphology is dramatically altered in mutants; the nuclei appear more uniform than wild type, have a less distinct chromocenter and lack a nucleolar region of low DNA density.

(Tulin et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Parp[+]/Parp1^CH1 is a suppressor of decreased cell number | adult stage phenotype of Pink1^B9

(Lehmann et al., 2017)

Parp[+]/Parp1^CH1 is a suppressor | partially of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Lehmann et al., 2017)

Parp[+]/Parp1^CH1 is a suppressor | partially of short lived phenotype of Pink1^B9

(Lehmann et al., 2017)

Parp[+]/Parp1^CH1 is a suppressor of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(Lehmann et al., 2017)

Parp[+]/Parp1^CH1 is a suppressor of short lived phenotype of park²⁵

(Lehmann et al., 2016)

Parp[+]/Parp1^CH1 is a suppressor of visible phenotype of park²⁵

(Lehmann et al., 2016)

Parp[+]/Parp1^CH1 is a suppressor of abnormal neuroanatomy | adult stage phenotype of park²⁵

(Lehmann et al., 2016)

Parp[+]/Parp1^CH1 is a suppressor of abnormal locomotor behavior | adult stage phenotype of park²⁵

(Lehmann et al., 2016)

Phenotype Manifest In

Suppressor of

Statement

Reference

Parp[+]/Parp1^CH1 is a suppressor | partially of adult thorax phenotype of Pink1^B9

(Lehmann et al., 2017)

Parp[+]/Parp1^CH1 is a suppressor | partially of adult brain phenotype of Pink1^B9

(Lehmann et al., 2017)

Parp[+]/Parp1^CH1 is a suppressor | partially of neuropil | adult stage phenotype of Pink1^B9

(Lehmann et al., 2017)

Parp[+]/Parp1^CH1 is a suppressor | partially of mitochondrial crista | adult stage phenotype of Pink1^B9

(Lehmann et al., 2017)

Parp[+]/Parp1^CH1 is a suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Pink1^B9

(Lehmann et al., 2017)

Parp[+]/Parp1^CH1 is a suppressor of dopaminergic PPL1 neuron phenotype of park²⁵

(Lehmann et al., 2016)

Parp[+]/Parp1^CH1 is a suppressor of adult thorax phenotype of park²⁵

(Lehmann et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Parp^CH1 heterozygosity leads to a significant suppression of the defects in lifespan, climbing capacity, thorax morphology, number of dopaminergic PPL1 neurons, mitochondria cristae morphology in the neuropil and oxygen consumption, observed in Pink1^B9 homozygous mutant adults; Parp^CH1 also rescues the decreased mitochodria potential observed in the brains of Pink1^B9 homozygous mutant adults.

(Lehmann et al., 2017)

park²⁵, Parp^CH1/+ double mutants display a reduced thoracic defect, and partially rescued climbing performance and survival, as compared to park²⁵ mutants. The dopaminergic neuron phenotype of park²⁵ is fully rescued in park²⁵, Parp^CH1/+ double mutants.

(Lehmann et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Parp1^CH4

(Tulin et al., 2002)

Rescued by

Parp1^CH1 is rescued by Scer\GAL4^arm.PS/Parp1^e.UAS

(Tulin et al., 2002)

Partially rescued by

Parp1^CH1 is partially rescued by Scer\GAL4^arm.PS/Parp1^I.UAS

(Tulin et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

81887

P{PZ}Parp1^CH1 ry⁵⁰⁶/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Excision of the P{PZ} element can revert the lethal phenotype.

(Tulin et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Parp1^CH1

Parp^CH1

(Goupil et al., 2022, Lehmann et al., 2017, Lehmann et al., 2016, Ong et al., 2013, Boamah et al., 2012, Pinnola et al., 2007)

parp^CH1

(Yu et al., 2021, Kotova et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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